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1. 发明授权

US08110406B2 Analytical method and analytical apparatus 失效
标题翻译：分析方法和分析仪器
公开(公告)号：US08110406B2
公开(公告)日：2012-02-07
申请号：US12085466
申请日：2006-11-27
申请人： Eiichi Tamiya , Naoki Nagatani , Teruko Yui , Tatsurou Endou , Ryou Tanaka
发明人： Eiichi Tamiya , Naoki Nagatani , Teruko Yui , Tatsurou Endou , Ryou Tanaka
IPC分类号： G01N33/53
CPC分类号： G01N33/54393 , Y10S435/97
摘要： [PROBLEMS] To reduce the amount of, for example, a capturing antibody to be employed without lowering detection sensitivity. At the same time, to enable the achievement of intense color development or light emission in a determination area even in the case where only a small amount of a labeled antibody is accumulated. To lower the detection limit in the sandwich method. To enlarge the dynamic range in the competition method. [MEANS FOR SOLVING PROBLEMS] A method of analyzing a test substance by an immunological analysis method by using the test substance, a support having a determination area, on which one member selected from a capturing antibody capable of binding specifically to the test substance and a capturing antigen capable of binding specifically to the test substance has been immobilized, and a labeled antibody capable of binding specifically to the test substance, wherein a label having a sensitizing effect has been immobilized on the determination area of the support.
摘要翻译： [问题]减少例如所用的捕获抗体的量而不降低检测灵敏度。同时，即使在只有少量的标记抗体被累积的情况下，也可以在确定区域中实现强烈的显色或发光。降低夹心法的检测限。在比赛方式中扩大动态范围。用于解决问题的手段通过使用被检物质的免疫学分析方法分析检测物质的方法，具有测定区域的载体，其中选自能够特异性结合测试物质的捕获抗体中的一个和已经固定了能够特异性结合测试物质的捕获抗原，并且能够特异性结合测试物质的标记抗体，其中具有敏化作用的标签已被固定在载体的确定区域上。

2. 发明申请

US20090047746A1 Analytical Method and Analytical Apparatus 失效
标题翻译：分析方法和分析仪器
公开(公告)号：US20090047746A1
公开(公告)日：2009-02-19
申请号：US12085466
申请日：2006-11-27
申请人： Eiichi Tamiya , Naoki Nagatani , Teruko Yui , Tatsurou Endou , Ryou Tanaka
发明人： Eiichi Tamiya , Naoki Nagatani , Teruko Yui , Tatsurou Endou , Ryou Tanaka
IPC分类号： G01N33/543 , G01N21/00
CPC分类号： G01N33/54393 , Y10S435/97
摘要： [PROBLEMS] To reduce the amount of, for example, a capturing antibody to be employed without lowering detection sensitivity. At the same time, to enable the achievement of intense color development or light emission in a determination area even in the case where only a small amount of a labeled antibody is accumulated. To lower the detection limit in the sandwich method. To enlarge the dynamic range in the competition method. [MEANS FOR SOLVING PROBLEMS] A method of analyzing a test substance by an immunological analysis method by using the test substance, a support having a determination area, on which one member selected from a capturing antibody capable of binding specifically to the test substance and a capturing antigen capable of binding specifically to the test substance has been immobilized, and a labeled antibody capable of binding specifically to the test substance, wherein a label having a sensitizing effect has been immobilized on the determination area of the support.
摘要翻译： [问题]减少例如所用的捕获抗体的量而不降低检测灵敏度。同时，即使在只有少量的标记抗体被累积的情况下，也可以在确定区域中实现强烈的显色或发光。降低夹心法的检测限。在比赛方式中扩大动态范围。用于解决问题的手段通过使用被检物质的免疫学分析方法分析检测物质的方法，具有测定区域的载体，其中选自能够特异性结合测试物质的捕获抗体中的一个和已经固定了能够特异性结合测试物质的捕获抗原，并且能够特异性结合测试物质的标记抗体，其中具有敏化作用的标签已被固定在载体的确定区域上。

3. 发明申请

US20090159458A1 Method for Determination of Test Substance 审中-公开
标题翻译：测定物质的测定方法
公开(公告)号：US20090159458A1
公开(公告)日：2009-06-25
申请号：US12226060
申请日：2007-03-29
申请人： Eiichi Tamiya , Naoki Nagatani , Teruko Yuhi , Kagan Kerman , Koutarou Idegami , Miyuki Chikae
发明人： Eiichi Tamiya , Naoki Nagatani , Teruko Yuhi , Kagan Kerman , Koutarou Idegami , Miyuki Chikae
IPC分类号： G01N27/416 , G01N33/543
CPC分类号： G01N33/5438
摘要： [Problems] A highly sensitive and accurate determination of a test substance is achieved without the need of complicated procedures.[Means for solving the Problems] The presence or absence or the concentration of a test substance 3 can be determined by gathering around the surface of a working electrode 1 metal microparticles 5 in an amount corresponding to the amount of the test substance 3 contained in a test solution, oxidizing the metal microparticles 5 electrochemically, measuring the value of a current produced by electrochemically reducing the oxidized metal, and determining the presence or absence or the concentration of the substance 3 based on the current value. It is preferred that the electrochemical oxidation of the metal microparticles 5 be conducted while maintaining the potential of the working electrode 1 at a value equal to a potential employed for the electrochemical oxidization of the metal microparticles 5.
摘要翻译： [问题]在不需要复杂程序的情况下实现对测试物质的高度灵敏和准确的测定。 [解决问题的方法]测试物质3的存在或不存在或浓度可以通过将工作电极1的金属微粒5的表面聚集在与金属微粒5中包含的测试物质3的量相当的量测试溶液，电化学氧化金属微粒5，测量通过电化学还原氧化金属产生的电流的值，并根据当前值确定物质3的存在或不存在或浓度。优选的是，在将工作电极1的电位保持在与金属微粒5的电化学氧化的电位相等的值的情况下，进行金属微粒5的电化学氧化。

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