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    • 4. 发明授权
    • Ion-detecting microspheres
    • 离子检测微球及其使用方法
    • US07651858B2
    • 2010-01-26
    • US11779173
    • 2007-07-17
    • Eric BakkerMartin Telting-DiazMike Bell
    • Eric BakkerMartin Telting-DiazMike Bell
    • G01N33/20G01N33/52
    • G01N33/84
    • This invention provides methods of using ion-detecting microspheres containing an ionphore and a chromoionphore in clinical laboratory instrumentation such as flow cytometry for sample analysis. In one embodiment, the microspheres are contacted with a flowing stream of a sample under conditions that allow the ion-selective ionophores to complex with the ions in the sample, and to cause deprotonation of the chromoionophore. The complexes are then exposed to an excitation wavelength light source suitable for exciting the deprotonated chromoionophore to emit a fluorescence signal pattern. Detection of the fluorescence signal pattern emitted by the deprotonated chromoionophore in microspheres containing the complexes allows for determination of the presence of the target ions in the sample. In one embodiment, lead ion-detecting microspheres are provided that can detect nanomolar levels of lead ions with response times on the order of minutes.
    • 本发明提供了在临床实验室仪器中使用含有离子光谱和chromoionphore的离子检测微球的方法,如流式细胞仪进行样品分析。 在一个实施方案中,使微球与允许离子选择性离子载体与样品中的离子络合的条件和样品的流动流接触,并引起染色质团的去质子化。 然后将复合物暴露于适合于激发去质子化的染色体以发射荧光信号图案的激发波长光源。 检测由含有复合物的微球中去质子化的染色体发射的荧光信号图案可以测定样品中目标离子的存在。 在一个实施方案中,提供可以以几分钟的响应时间检测纳摩尔含量的铅离子的铅离子检测微球。
    • 5. 发明授权
    • Ion-detecting microspheres and methods of use thereof
    • 离子检测微球及其使用方法
    • US07247489B2
    • 2007-07-24
    • US10384082
    • 2003-03-07
    • Eric BakkerMartin Telting-DiazMike Bell
    • Eric BakkerMartin Telting-DiazMike Bell
    • G01N33/20G01N33/52
    • G01N33/84
    • This invention provides methods of using ion-detecting microspheres containing an ionphore and a chromoionphore in clinical laboratory instrumentation such as flow cytometry for sample analysis. In one embodiment, the microspheres are contacted with a flowing stream of a sample under conditions that allow the ion-selective ionophores to complex with the ions in the sample, and to cause deprotonation of the chromoionophore. The complexes are then exposed to an excitation wavelength light source suitable for exciting the deprotonated chromoionophore to emit a fluorescence signal pattern. Detection of the fluorescence signal pattern emitted by the deprotonated chromoionophore in microspheres containing the complexes allows for determination of the presence of the target ions in the sample. In one embodiment, lead ion-detecting microspheres are provided that can detect nanomolar levels of lead ions with response times on the order of minutes.
    • 本发明提供了在临床实验室仪器中使用含有离子光谱和chromoionphore的离子检测微球的方法,如流式细胞仪进行样品分析。 在一个实施方案中,使微球与允许离子选择性离子载体与样品中的离子络合的条件和样品的流动流接触,并引起染色质团的去质子化。 然后将复合物暴露于适合于激发去质子化的染色体以发射荧光信号图案的激发波长光源。 检测由含有复合物的微球中去质子化的染色体发射的荧光信号图案可以测定样品中目标离子的存在。 在一个实施方案中,提供可以以几分钟的响应时间检测纳摩尔含量的铅离子的铅离子检测微球。
    • 7. 发明授权
    • Coin dispensing apparatus
    • 硬币分配装置
    • US06273809B1
    • 2001-08-14
    • US09147455
    • 1998-12-29
    • Mike BellColin Richard SpoonerBrian John Spooner
    • Mike BellColin Richard SpoonerBrian John Spooner
    • G07D100
    • G07D1/00G07D9/008
    • Coin dispensing apparatus is provided including a reservoir for coins and a rotor located to receive coins from the reservoir, the rotor having a lower storage portion shaped to receive a stack of coins, and an upper feed portion shaped to receive coins from the reservoir and direct them to the lower storage portion. Because the rotor receives and feeds the coins, to provide a stack of coins within the lower storage portion, ready for dispensing, the associated reservoir can be of a size to receive any type of coin. If it is necessary to adapt the apparatus to accept a different type of coin, it is only necessary to replace the rotor.
    • 提供了硬币分配装置,其包括用于硬币的储存器和用于从储存器接收硬币的转子,转子具有形状为接收一堆硬币的下部存储部分,以及形成为从储存器接收硬币的上部进料部分, 它们到下部存储部分。 因为转子接收和进给硬币,以在下部存储部分内提供一堆硬币,准备分配,相关联的储存器可以具有接收任何类型的硬币的尺寸。 如果需要使装置适应不同类型的硬币,则仅需要更换转子。