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1. 发明授权

US08748102B2 Bead emulsion nucleic acid amplification 有权
公开(公告)号：US08748102B2
公开(公告)日：2014-06-10
申请号：US13033240
申请日：2011-02-23
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

2. 发明申请

US20110166027A1 INTERACTION SCREENING METHODS, SYSTEMS AND DEVICES 审中-公开
标题翻译：交互式筛选方法，系统和设备
公开(公告)号：US20110166027A1
公开(公告)日：2011-07-07
申请号：US12674374
申请日：2008-08-21
申请人： Michael P. Weiner
发明人： Michael P. Weiner
IPC分类号： C40B20/04 , C40B30/04 , C12N1/00
CPC分类号： G01N33/5302 , G01N33/54366
摘要： An interaction screening method for identifying binding moieties encapsulates candidate binding moieties in droplets with a first known moiety and a second known moiety. The candidate binding moieties are different in the different droplets. The method further comprises determining for one or more of the droplets whether the candidate binding moiety is bound to the first known moiety and/or the second known moiety. Optionally, the method further comprises segregating at least one droplet in which the candidate binding moiety is bound to the first known moiety or to the first and second known moiety.
摘要翻译：用于鉴定结合部分的相互作用筛选方法用第一已知部分和第二已知部分将候选结合部分包封在液滴中。候选结合部分在不同的液滴中是不同的。所述方法还包括确定所述液滴中的一个或多个，所述候选结合部分是否与第一已知部分和/或第二已知部分结合。任选地，该方法进一步包括将候选结合部分与第一已知部分或第一和第二已知部分结合的至少一个液滴分离。

3. 发明授权

US07335762B2 Apparatus and method for sequencing a nucleic acid 失效
标题翻译：用于测序核酸的装置和方法
公开(公告)号：US07335762B2
公开(公告)日：2008-02-26
申请号：US10222592
申请日：2002-08-15
申请人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo , Michael P. Weiner
发明人： Jonathan M. Rothberg , Joel S. Bader , Scott B. Dewell , Keith McDade , John W. Simpson , Jan Berka , Christopher M. Colangelo , Michael P. Weiner
IPC分类号： C07H21/04 , C12Q1/68 , C12P19/34
CPC分类号： G01N21/6452 , B01L3/5027 , B01L3/502715 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B01L2300/0893 , B82Y15/00 , B82Y30/00 , C12P19/34 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , G01N21/0303 , G01N21/7703 , C12Q2565/519 , C12Q2533/101 , C12Q2531/125 , C12Q2535/101 , C12Q2565/301 , C12Q2565/107 , C12Q2525/307
摘要： Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.
摘要翻译：本文公开了用于测序核酸的方法和装置。这些方法允许非常大量的独立测序反应并行排列，允许非常大数量（> 10,000）不同寡核苷酸的同时测序。

4. 发明授权

US07244567B2 Double ended sequencing 失效
标题翻译：双端测序
公开(公告)号：US07244567B2
公开(公告)日：2007-07-17
申请号：US10768729
申请日：2004-01-28
申请人： Yi-Ju Chen , John H. Leamon , Kenton L. Lohman , Michael T. Ronan , Jonathan M. Rothberg , Maithreyan Srinivasan , Michael P. Weiner
发明人： Yi-Ju Chen , John H. Leamon , Kenton L. Lohman , Michael T. Ronan , Jonathan M. Rothberg , Maithreyan Srinivasan , Michael P. Weiner
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.
摘要翻译：本发明涉及DNA测序方法。更具体地，本发明涉及通过使用封闭和未封闭的测序引物对DNA的有义链和反义链进行测序的方法。简而言之，这些方法包括将未封闭引物退火至第一条核酸的步骤; 将第二封闭引物退火至核酸的第二链; 用聚合酶沿着第一链延长核酸; 终止第一个测序引物; 解封第二底漆; 并沿着第二链延长核酸。

5. 发明授权

US09200274B2 Expanded radix for polymeric tags 有权
标题翻译：聚合物标签的膨胀基
公开(公告)号：US09200274B2
公开(公告)日：2015-12-01
申请号：US14359558
申请日：2012-11-19
申请人： Michael P. Weiner
发明人： Michael P. Weiner
IPC分类号： C12N15/10 , C12Q1/68 , C40B20/00 , C40B30/00
CPC分类号： C12N15/1065 , C12Q1/6816 , C12Q1/6869 , C12Q1/6874 , C40B20/00 , C40B30/00 , C12Q2563/179 , C12Q2563/185
摘要： A method having steps of (a) providing nucleic acids having a tag sequence (N1)n(N2)n . . . (Nx)n, wherein N1, N2 and Nx are nucleotides that complement different nucleotides, respectively, wherein n is an integer that can differ for N1, N2 and Nx; (b) detecting the nucleic acids individually and under conditions to distinguish signal intensities for (N1)n sequences having different values for n, (N2)n sequences having different values for n and. (Nx)n sequences having different values for n; and (c) distinguishing the tags based on the signal intensities.
摘要翻译：一种具有以下步骤的方法：（a）提供具有标签序列（N1）n（N 2）n的核酸。。。（Nx）n，其中N1，N2和Nx分别是互补不同核苷酸的核苷酸，其中n是对于N1，N2和Nx可以不同的整数; （b）分别检测核酸，并在条件下检测具有不同n值的n，（N 2）n个序列的（N1）n个序列的信号强度的信号强度。（Nx）n个序列具有不同的n值; 和（c）基于信号强度区分标签。

6. 发明授权

US08790876B2 Methods of amplifying and sequencing nucleic acids 有权
标题翻译：扩增和测序核酸的方法
公开(公告)号：US08790876B2
公开(公告)日：2014-07-29
申请号：US11788838
申请日：2007-04-20
申请人： John H. Leamon , Kenton L. Lohman , Jonathan M. Rothberg , Michael P. Weiner
发明人： John H. Leamon , Kenton L. Lohman , Jonathan M. Rothberg , Michael P. Weiner
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译：本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。

7. 发明申请

US20130059741A1 BINDING ASSAYS FOR MARKERS 审中-公开
标题翻译：标记的绑定测试
公开(公告)号：US20130059741A1
公开(公告)日：2013-03-07
申请号：US13696848
申请日：2011-05-13
申请人： Michael P. Weiner
发明人： Michael P. Weiner
IPC分类号： C40B20/04 , C40B40/06
CPC分类号： C12Q1/6804 , G01N33/54313 , G01N33/58 , G01N2458/10 , C12Q2565/543
摘要： This invention provides compositions and methods for assaying the presence of a target analyte in a sample using a solid support. Embodiments of the present invention provide a solid support having a binding protein, such as an antibody, antibody fragment or protein receptor, immobilized to the solid support and at least two separate nucleic acid primers immobilized near the binding protein. This invention also provides a method for tethering two or more polypeptide subunits to generate a multifunctional fusion protein, which can have a primary function, e.g., binding a target analyte, such as a target protein, or an enzymatic activity, and one or more of the subunits of the fusion protein carries out a secondary function, e.g., capture on a solid matrix or quantitation.
摘要翻译：本发明提供了使用固体支持物来测定样品中目标分析物的存在的组合物和方法。本发明的实施方案提供了固体支持物，其具有固定在固体支持物上的结合蛋白，例如抗体，抗体片段或蛋白受体，以及固定在结合蛋白附近的至少两个分开的核酸引物。本发明还提供了一种用于束缚两个或更多个多肽亚基以产生多功能融合蛋白的方法，所述多功能融合蛋白可具有主要功能，例如结合目标分析物如靶蛋白或酶活性，以及一种或多种融合蛋白的亚基执行次要功能，例如在固体基质上捕获或定量。

8. 发明申请

US20120238475A1 Methods of Amplifying and Sequencing Nucleic Acids 审中-公开
标题翻译：核酸的扩增和测序方法
公开(公告)号：US20120238475A1
公开(公告)日：2012-09-20
申请号：US13419241
申请日：2012-03-13
申请人： John H. Leamon , Kenton L. Lohman , Jonathan M. Rothberg , Michael P. Weiner
发明人： John H. Leamon , Kenton L. Lohman , Jonathan M. Rothberg , Michael P. Weiner
IPC分类号： C40B40/06
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译：本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。

9. 发明申请

US20110143955A1 Protein Capture, Detection and Quantitation 审中-公开
标题翻译：蛋白质捕获，检测和定量
公开(公告)号：US20110143955A1
公开(公告)日：2011-06-16
申请号：US12902379
申请日：2010-10-12
申请人： Michael P. Weiner
发明人： Michael P. Weiner
IPC分类号： C40B30/04 , C40B40/10
CPC分类号： G01N33/5308 , C12Q1/6804 , C12Q2563/179 , C12Q2521/501
摘要： This invention is related to the area of the capture, detection and quantitation of proteins. In particular, it relates to making and using recombinant antibodies bound to oligonucleotides and using such complexes for analytic purposes. These techniques are designed to permit multiplexed detection and quantitation of very large numbers of proteins.
摘要翻译：本发明涉及蛋白质的捕获，检测和定量的面积。特别地，本发明涉及制备和使用与寡核苷酸结合的重组抗体并使用这种复合物用于分析目的。这些技术被设计为允许多次检测和定量非常大量的蛋白质。

10. 发明申请

US20080132693A1 Bead emulsion nucleic acid amplification 无效
标题翻译：珠粒乳液核酸扩增
公开(公告)号：US20080132693A1
公开(公告)日：2008-06-05
申请号：US11982095
申请日：2007-10-31
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
IPC分类号： C07H1/06 , C12P19/34 , G01N33/00
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
摘要翻译：公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。还公开了用于执行本发明的方法的套件和装置。

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