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1. 发明申请

US20160304925A1 ENZYME BASED METHODS OF SEPARATING PROTEIN FROM PROTEIN-RICH MATERIAL 审中-公开
标题翻译：基于酶的蛋白质从蛋白质分离分离蛋白质的方法
公开(公告)号：US20160304925A1
公开(公告)日：2016-10-20
申请号：US15133777
申请日：2016-04-20
申请人： Lu-Kwang Ju
发明人： Lu-Kwang Ju
IPC分类号： C12P19/14 , G06F19/24 , C12P19/02
摘要： Improved enzyme based methods of separating protein from protein-rich material are provided. A method can include utilizing a modeling equation to more effectively hydrolyze the various types of carbohydrates present in a protein-rich material. A method can include a fed-batch method of incrementally adding a protein-rich material, an enzyme broth, or both a protein-rich material and an enzyme broth. A method can also include partially or completely recycling the hydrolysate.
摘要翻译：提供了将蛋白质与富含蛋白质的材料分离的基于酶的方法的改进。一种方法可以包括利用建模方程来更有效地水解富含蛋白质的材料中存在的各种类型的碳水化合物。一种方法可以包括逐步添加富含蛋白质的材料，酶肉汤或富含蛋白质的材料和酶肉汤两者的补料分批方法。一种方法还可以包括部分或完全循环水解产物。

2. 发明申请

US20160102007A1 PRODUCING ALGAL BIOMASS AND PRODUCTS FROM ORGANIC SOLID MATERIAL 有权
标题翻译：从有机固体材料生产ALGAL生物质和产品
公开(公告)号：US20160102007A1
公开(公告)日：2016-04-14
申请号：US14893160
申请日：2014-05-30
申请人： Lu-Kwang JU , Cong LI , Suo XIAO
发明人： Lu-Kwang Ju , Cong Li , Suo Xiao
IPC分类号： C02F11/02 , A01G33/00
CPC分类号： C02F11/02 , A01G33/00 , C02F1/36 , C02F3/006 , C02F3/28 , C02F3/322 , C02F2209/001 , C02F2209/003 , C02F2209/02 , C02F2209/06 , C02F2209/14 , C02F2209/22 , C02F2209/40 , C02F2209/42 , C02F2209/44 , C02F2305/06 , Y02A40/88
摘要： A method for treating solid organic materials includes providing phagotrophic algae, providing solid organic material, combining the algae and the solid organic material, allowing the algae to grow by engulfing or uptaking the solid organic material, forming an algal product, and collecting the algal product. The method can also include a pretreatment step. The solid organic material can be waste activated sludge. A system for treating and disposing solid organic material is also provided.
摘要翻译：用于处理固体有机材料的方法包括提供富营养藻类，提供固体有机物质，将藻类和固体有机物质结合，使藻类通过吞噬或吸收固体有机物质，形成藻类产品并收集藻类产品而生长。该方法还可以包括预处理步骤。固体有机物可以是废活性污泥。还提供了一种用于处理和处理固体有机材料的系统。

3. 发明授权

US09062329B2 Production of arabital 有权
标题翻译：生产阿拉伯语
公开(公告)号：US09062329B2
公开(公告)日：2015-06-23
申请号：US13811298
申请日：2011-07-22
申请人： Lu-Kwang Ju
发明人： Lu-Kwang Ju
IPC分类号： C07C31/18 , C12P7/18 , C12P7/20 , C12N1/00
CPC分类号： C12P7/18 , C07C31/18 , C12P7/20
摘要： A method for producing arabitol, and more particularly to producing arabitol in a major amount based on a total weight of all polyols produced and in relatively high concentration from a mixture including a carbon source such as glycerol. The method includes in one embodiment utilizing select yeast strains to produce arabitol in high yield while minimizing the amounts of other polyols, using carbon sources such as glycerol as a component in a medium. In a beneficial embodiment, biodiesel byproduct glycerol is used as the substrate for arabitol production.
摘要翻译：一种生产阿拉伯糖醇的方法，更具体地涉及以所产生的所有多元醇的总重量为主要成分的阿糖醇，并且从包含碳源如甘油的混合物中以较高的浓度生产阿拉伯糖醇。该方法包括在一个实施方案中利用选择酵母菌株以高产率生产阿拉伯糖醇，同时使用碳源（例如甘油作为培养基中的成分）最小化其它多元醇的量。在有利的实施方案中，使用生物柴油副产物甘油作为阿拉伯糖醇生产的底物。

4. 发明申请

US20110027858A1 Method of Modifying Fungal Morphology 审中-公开
标题翻译：改良真菌形态的方法
公开(公告)号：US20110027858A1
公开(公告)日：2011-02-03
申请号：US12845321
申请日：2010-07-28
申请人： Lu-Kwang Ju , Narayanan Srinivasan , Nicholas V. Callow
发明人： Lu-Kwang Ju , Narayanan Srinivasan , Nicholas V. Callow
IPC分类号： C12N9/42 , C12N1/14
CPC分类号： C12N1/14
摘要： Provided is a method of modifying fungal morphology. The method comprises introducing an amount of a surfactant to a fungus, or to a growing mixture comprising a fungus, wherein said amount of a surfactant is sufficient to induce a change in the morphology of said fungus.
摘要翻译：提供了一种改变真菌形态的方法。该方法包括将一定量的表面活性剂引入到真菌中，或向包含真菌的生长混合物中引入，其中所述量的表面活性剂足以诱导所述真菌的形态变化。

5. 发明授权

US06413763B1 Method of removing gas from a site using gas vesicles of cells 有权
标题翻译：使用细胞气泡从现场除去气体的方法
公开(公告)号：US06413763B1
公开(公告)日：2002-07-02
申请号：US09489386
申请日：2000-01-21
申请人： Lu-Kwang Ju , Anand Sundararajan , Sunil Kashyap
发明人： Lu-Kwang Ju , Anand Sundararajan , Sunil Kashyap
IPC分类号： A61B800
CPC分类号： C12M33/00 , A61K47/6925 , C12M29/20 , C12N1/00 , C12N1/02 , C12N1/12 , C12N1/20 , Y10S435/822 , Y10S435/832 , Y10S435/946
摘要： A method for removing a gas compound from a site comprising placing cells having gas vesicles under conditions that induce the cells to float to a surface of an aqueous medium, harvesting the cells from the surface of the medium, lysing the cells, separating the gas vesicles from the lysed cells, crosslinking the gas vesicles with a crosslinking agent, loading a gas with a lowered partial pressure for the gas compound to be removed into the gas vesicles, and placing the gas vesicles contacted with a medium such that the gas compound is removed from the site to the medium. Harvesting gas-vesicle-containing cells is achieved by placing the cells under conditions that induce the cells to rise to the surface of an aqueous medium. Then collecting the cells from the surface of the medium. Gas vesicles are isolated by lysing the cells and separating the gas vesicles from the lysate. Once the gas vesicles are isolated, they can be modified, such as by crosslinking with glutaraldehyde.
摘要翻译：一种从一个位置除去气体化合物的方法，包括在诱导细胞漂浮到水性介质表面的条件下放置具有气泡的细胞，从培养基表面收集细胞，裂解细胞，分离气泡从裂解的细胞中，用交联剂交联气泡，加载具有降低的分压的气体，以将气体化合物除去到气泡中，并将气泡与介质接触以使气体化合物被除去从现场到中等。通过将细胞置于诱导细胞升至水性介质表面的条件下来实现收获含气泡囊泡细胞。然后从培养基表面收集细胞。通过裂解细胞并从溶胞产物中分离出气体囊泡来分离气泡。一旦气体囊泡被分离，它们可以被改变，例如通过与戊二醛交联。

6. 发明授权

US10696993B2 Enzyme based methods of separating protein from protein-rich material 有权
公开(公告)号：US10696993B2
公开(公告)日：2020-06-30
申请号：US15133777
申请日：2016-04-20
申请人： Lu-Kwang Ju , S M Mahfuzul Islam , Qian Li , Abdullah Al Loman
发明人： Lu-Kwang Ju , S M Mahfuzul Islam , Qian Li , Abdullah Al Loman
IPC分类号： C12P19/14 , C12P19/02
摘要： Improved enzyme based methods of separating protein from protein-rich material are provided. A method can include utilizing a modeling equation to more effectively hydrolyze the various types of carbohydrates present in a protein-rich material. A method can include a fed-batch method of incrementally adding a protein-rich material, an enzyme broth, or both a protein-rich material and an enzyme broth. A method can also include partially or completely recycling the hydrolysate.

7. 发明授权

US10428355B2 Production of arabitol 有权
公开(公告)号：US10428355B2
公开(公告)日：2019-10-01
申请号：US15648806
申请日：2017-07-13
申请人： Lu-Kwang Ju , Abdullah Al Loman
发明人： Lu-Kwang Ju , Abdullah Al Loman
IPC分类号： C12P7/18
摘要： A method for producing arabitol may include providing a fermentation culture having a microorganism and a carbon source; allowing the microorganism to ferment the carbon source; monitoring a process condition of said step of allowing the microorganism to ferment the carbon source; and collecting a product from the fermentation culture after said step of monitoring a process condition indicates that a predetermined change in the process condition has occurred. Other methods may include steps of providing soybean-based lignocellulosic hydrolysate as a carbon source for a fermentation culture, and modifying the pH of one or more of the growth phase and the stationary phase of a fermentation process.

8. 发明申请

US20170265468A1 RHAMNOLIPID BASED BIOPESTICIDES 有权
公开(公告)号：US20170265468A1
公开(公告)日：2017-09-21
申请号：US15463173
申请日：2017-03-20
申请人： Lu-Kwang Ju , Ashwin Sancheti
发明人： Lu-Kwang Ju , Ashwin Sancheti
IPC分类号： A01N43/16
CPC分类号： A01N43/16 , A01N25/00
摘要： A biopesticide composition includes a solvent and a plurality of rhamnolipid complexes, each rhamnolipid complex including a rhamnolipid complexed with a cation. The biopesticide composition may include a slower-releasing rhamnolipid-based material and a faster-releasing rhamnolipid-based material. The biopesticide composition may be in the form of a coating or a pellet.

9. 发明申请

US20150336066A1 METHOD OF ENCAPSULATION AND IMMOBILIZATION 审中-公开
标题翻译：封装和固定方法
公开(公告)号：US20150336066A1
公开(公告)日：2015-11-26
申请号：US14819822
申请日：2015-08-06
申请人： Lu-Kwang Ju , Uchechukwu Anozie
发明人： Lu-Kwang Ju , Uchechukwu Anozie
IPC分类号： B01J13/18 , B01J13/20
CPC分类号： B01J13/18 , B01J13/20 , B01J13/206 , C05D9/02 , C05G3/0017 , C05G3/0047 , Y10T428/2989
摘要： A method for encapsulating a material, the steps include choosing a material to encapsulate; placing the material into a material solvent to form a material solution; forming a primary emulsion of the material solution in an immiscible liquid medium that is immiscible with the material solvent, wherein the material solution is the disperse phase and the immiscible liquid medium is the continuous phase of the primary emulsion, wherein the immiscible liquid medium contains an encapsulating agent dissolved therein, the encapsulating agent capable of being crosslinked, polymerized, gelled, hardened or solidified; adding the primary emulsion as droplets into a crosslinking medium, wherein the crosslinking medium is immiscible with the continuous phase immiscible liquid medium of the primary emulsion, and thereafter activating the crosslinking, polymerizing, gelling, hardening or solidifying of the encapsulating agent to envelope the material in a crosslinked, polymerized, gelled, hardened or solidified matrix forming the droplets into beads.
摘要翻译：一种封装材料的方法，所述步骤包括选择要封装的材料; 将材料放入材料溶剂中以形成材料溶液; 在与所述材料溶剂不混溶的不混溶液体介质中形成所述材料溶液的主乳液，其中所述材料溶液是分散相，并且所述不混溶液体介质是所述主乳液的连续相，其中所述不混溶液体介质包含溶解在其中的包封剂，能够交联，聚合，凝胶化，硬化或固化的包封剂; 将初级乳液作为液滴加入交联介质中，其中交联介质与初级乳液的连续相不混溶液体介质不混溶，然后活化封装剂的交联，聚合，凝胶化，固化或固化以包封材料在交联的，聚合的，胶凝的，硬化的或固化的基质中形成液滴成珠。

10. 发明申请

US20140038247A1 ALGAE HAVING INTRACELLULAR LIPID PARTICLES AND HIGH LIPID CONTENT 审中-公开
标题翻译：具有细胞内脂质颗粒和高脂含量
公开(公告)号：US20140038247A1
公开(公告)日：2014-02-06
申请号：US13959098
申请日：2013-08-05
申请人： Lu-Kwang Ju , Cong Li
发明人： Lu-Kwang Ju , Cong Li
IPC分类号： C12P7/64
CPC分类号： C12P7/649 , C12P7/6463 , Y02E50/13
摘要： A method for increasing the lipid content of algae includes combining algae and a lipid-precipitating addition in a growth-inhibitive medium, the growth-inhibitive medium being deficient in at least one nutrient that is necessary for reproductive growth of algae, thus frustrating algae reproduction, the lipid-precipitating addition selected from hydroxyl-containing compounds and amine-containing compounds. An organic acid addition may also be combined with the lipid-precipitating addition and algae in the growth-inhibitive medium, the organic acid addition including compounds containing carboxylic acid functionality. Direct production of biodiesel is also achieved by the use of particular lipid-precipitating additions and organic acid additions.
摘要翻译：增加藻类脂质含量的方法包括在生长抑制性培养基中组合藻类和脂质沉淀添加物，生长抑制性培养基缺乏藻类繁殖生长所必需的至少一种营养物质，从而使藻类繁殖失败，选自含羟基化合物和含胺化合物的脂质沉淀添加剂。有机酸添加也可以与生长抑制性介质中的脂质沉淀添加物和藻类组合，有机酸添加物包括含有羧酸官能团的化合物。通过使用特定的脂质沉淀添加剂和有机酸添加物也可以直接生产生物柴油。

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