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1. 发明授权

US6093535A Method for identifying attenuated chickenpox virus Oka strain or strain originating therein and acceptable as attenuated chickenpox vaccine virus 失效
标题翻译：用于鉴定减毒水痘病毒Oka菌株或由其衍生的菌株的方法
公开(公告)号：US6093535A
公开(公告)日：2000-07-25
申请号：US983045
申请日：1998-01-15
申请人： Chisato Mori , Rie Takahara , Juichiro Osame , Yasuyuki Gomi , Isao Fuke
发明人： Chisato Mori , Rie Takahara , Juichiro Osame , Yasuyuki Gomi , Isao Fuke
IPC分类号： A61K39/00 , C07K14/04 , C12N15/38 , C12Q1/70 , C07H21/04 , C12N7/00 , C12P19/34
CPC分类号： C07K14/005 , C12Q1/701 , A61K39/00 , C12N2710/16722
摘要： Disclosed is a method for exact identification of the attenuated varicella virus Oka strain or a strain derived therefrom capable of functioning as an attenuated varicella live vaccine virus, which comprises analyzing the difference in the genomic DNA and fragments thereof between the Oka strain and a sample varicella strain, and determining whether or not a sample strain satisfies all of the following eight characteristics: the sizes of the HpaI-K fragment and the EcoRI-P fragment; the size of R2-487 region of Gene14 and the analysis by PCR-SSCP; the sizes of the restriction fragments obtained by digesting the R2-1764 fragment with AccIII; the absence or presence of a PstI cleavage site; the homology of the amino acid sequences coded by R2-487 coding region; and the homology of the amino acid sequences coded by the coding region of VZV Gene14. The method of the present invention is extremely useful for the quality control and quality assurance of attenuated varicella live vaccines. Also disclosed are an isolated virus strain which is substantially the same virus strain as identified by the above method; an attenuated varicella virus live vaccine comprising the same virus strain as identified by the above method; an attenuated varicella virus Oka strain antigen; and a pair of primers which are advantageously, effectively usable in the above method.
摘要翻译： PCT No.PCT / JP97 / 01646 Sec。 371日期1998年1月15日 102（e）日期1998年1月15日PCT提交1997年5月15日PCT公布。公开号WO97 / 43420 PCT 日期1997年11月20日公开是一种精确鉴定减毒水痘病毒Oka菌株或由其衍生的能够作为减毒水痘活疫苗病毒的菌株的方法，其中包括分析Oka基因组DNA及其片段之间的差异菌株和样本水痘菌株，并确定样品菌株是否满足以下八个特征：HpaI-K片段和EcoRI-P片段的大小; Gene14的R2-487区域的大小和通过PCR-SSCP的分析; 通过用AccIII消化R2-1764片段获得的限制性片段的大小; 不存在或存在PstI切割位点; 由R2-487编码区编码的氨基酸序列的同源性; 和由VZV Gene14的编码区编码的氨基酸序列的同源性。本发明的方法对于减毒水痘活疫苗的质量控制和质量保证是非常有用的。还公开了分离的病毒株，其通过上述方法鉴定为基本上相同的病毒株; 包含由上述方法鉴定的相同病毒株的减毒水痘病毒活疫苗; 减毒水痘病毒Oka株抗原; 以及有利地在上述方法中有效使用的一对引物。

2. 发明授权

US6039958A Stabilized live vaccine 失效
标题翻译：稳定活疫苗
公开(公告)号：US6039958A
公开(公告)日：2000-03-21
申请号：US179970
申请日：1998-10-28
申请人： Kuniaki Koyama , Juichiro Osame
发明人： Kuniaki Koyama , Juichiro Osame
IPC分类号： A61K39/25 , A61K39/295 , A61P31/12 , A61P31/22 , A01N63/00 , A61K39/12
CPC分类号： A61K39/25 , A61K39/0015 , A61K39/12 , A61K2039/5254 , A61K2039/70 , C12N2710/16734
摘要： A stabilized live vaccine containing a varicella virus and a stabilizer, wherein the vaccine is substantially free of Ca.sup.2+ ions and Mg.sup.2+ ions is described. This stabilized live vaccine is excellent in storage stability and heat resistance. Also described is an improved stabilizer for a live varicella vaccine, comprising at least gelatin or hydrolyzed gelatin, each being substantially free of Ca.sup.2+ ions and Mg.sup.2+ ions. The stabilizer can advantageously be used to stabilize a live vaccine containing a varicella virus. The substantial freedom of Ca.sup.2+ ions and Mg.sup.2+ ions can be attained by masking Ca.sup.2+ ions and Mg.sup.2+ ions present in a live vaccine containing a varicella virus and a stabilizer, with a chelating reagent, or by using as a stabilizer gelatin and/or a gelatin derivative after being purified to remove Ca.sup.2+ ions and/or Mg.sup.2+ ions contained therein.
摘要翻译：含有水痘病毒和稳定剂的稳定的活疫苗，其中疫苗基本上不含Ca 2+离子，描述了Mg 2+离子。这种稳定的活疫苗具有优异的储存稳定性和耐热性。还描述了一种用于活水痘疫苗的改进的稳定剂，其包含至少明胶或水解明胶，其各自基本上不含Ca 2+离子和Mg 2+离子。稳定剂可以有利地用于稳定含有水痘病毒的活疫苗。通过用螯合剂掩蔽含有水痘病毒和稳定剂的活疫苗中的Ca 2+离子和Mg 2+离子，或通过使用明胶和/或明胶衍生物作为稳定剂，可以获得Ca 2+离子和Mg 2+离子的实质自由度经纯化以除去其中所含的Ca 2+离子和/或Mg 2+离子。

3. 发明授权

US5139776A Method for culturing Bordetella pertussis, a pertussis toxoid and a pertussis vaccine 失效
标题翻译：培养百日咳博德特氏菌，百日咳类毒素和百日咳疫苗的方法
公开(公告)号：US5139776A
公开(公告)日：1992-08-18
申请号：US305680
申请日：1989-02-03
申请人： Masashi Chazono , Iwao Yoshida , Takeo Konobe , Juichiro Osame , Keisuke Takaku
发明人： Masashi Chazono , Iwao Yoshida , Takeo Konobe , Juichiro Osame , Keisuke Takaku
IPC分类号： A61K39/10 , C12N1/20
CPC分类号： C12N1/20 , A61K39/099 , Y10S435/832
摘要： There is disclosed a method for culturing Bordetella pertussis in the presence of a cellulose and/or cellulose derivatives. The present method is useful for obtaining a mixed antigen comprising pertussis toxin and filamentous hemagglutinin in a large amount at low cost. From the antigen, there can be obtained a stable and effective pertussis toxoid to be used for a pertussis vaccine. There is also disclosed a vaccine comprising the pertussis toxoid as an active ingredient and a gelatin and/or gelatin derivatives as a stabilizing agent. The present vaccine is extremely stable and can be stored for a prolonged period of time.
摘要翻译：公开了一种在纤维素和/或纤维素衍生物存在下培养百日咳博德特氏菌的方法。本方法以低成本大量获得包含百日咳毒素和丝状血凝素的混合抗原是有用的。从抗原中可获得用于百日咳疫苗的稳定且有效的百日咳类毒素。还公开了包含百日咳类毒素作为活性成分的疫苗和明胶和/或明胶衍生物作为稳定剂。目前的疫苗非常稳定，可以长时间保存。

4. 发明授权

US4849358A Method for culturing bordetella pertussis, a pertussis toxoid and a pertussis vaccine 失效
标题翻译：培养百日盛博戈特氏菌的方法，百日咳类毒素和百日咳疫苗
公开(公告)号：US4849358A
公开(公告)日：1989-07-18
申请号：US073134
申请日：1987-07-14
申请人： Masashi Chazono , Iwao Yoshida , Takeo Konobe , Juichiro Osame , Keisuke Takaku
发明人： Masashi Chazono , Iwao Yoshida , Takeo Konobe , Juichiro Osame , Keisuke Takaku
IPC分类号： A61K39/00 , A61K39/10 , C07K14/235 , C12N1/20 , C12N1/22
CPC分类号： C12N1/22 , A61K39/099 , C07K14/235 , C12N1/20 , A61K39/00 , Y10S435/832
摘要： There is disclosed a method for culturing Bordetella pertussis in the prece of a cellulose and/or cellulose derivatives. The present method is useful for obtaining a mixed antigen comprising pertussis toxin and filamentous hemagglutinin in a large amount at low cost. From the antigen, there can be obtained a stable and effective pertussis toxoid to be used for a pertussis vaccine. There is also disclosed a vaccine comprising the pertussis toxoid as an active ingredient and a gelatin and/or gelatin derivatives as a stabilizing agent. The present vaccine is extremely stable and can be stored for a prolonged period of time.
摘要翻译：公开了一种在纤维素和/或纤维素衍生物存在下培养百日咳博德特氏菌的方法。本方法以低成本大量获得包含百日咳毒素和丝状血凝素的混合抗原是有用的。从抗原中可获得用于百日咳疫苗的稳定且有效的百日咳类毒素。还公开了包含百日咳类毒素作为活性成分的疫苗和明胶和/或明胶衍生物作为稳定剂。目前的疫苗非常稳定，可以长时间保存。

5. 发明授权

US5650153A Recombinant Marek's disease virus and vaccine 失效
标题翻译：重组马立克氏病病毒和疫苗
公开(公告)号：US5650153A
公开(公告)日：1997-07-22
申请号：US293337
申请日：1994-08-24
申请人： Toyokazu Ishikawa , Sadao Manabe , Chisato Mori , Akihisa Takamizawa , Iwao Yoshida , Juichiro Osame , Keisuke Takaku , Konosuke Fukai
发明人： Toyokazu Ishikawa , Sadao Manabe , Chisato Mori , Akihisa Takamizawa , Iwao Yoshida , Juichiro Osame , Keisuke Takaku , Konosuke Fukai
IPC分类号： C12N15/09 , A61K39/00 , A61K39/17 , A61K39/255 , C07K14/055 , C07K14/125 , C07K14/165 , C12N7/00 , C12N15/869 , C12R1/92 , A61K39/265 , C12N15/86
CPC分类号： C12N15/86 , C07K14/005 , A61K39/00 , C12N2710/16322 , C12N2710/16343 , C12N2760/18122 , C12N2770/20022
摘要： There is provided a recombinant Marek's disease virus comprising the genomic DNA of an attenuated Marek's disease virus and a foreign gene from another source. The present recombinant virus can advantageously be used as an active ingredient for a multifunctional live vaccine, exhibiting not only the antigenicity and immunogenicity of the Marek's disease virus but also the properties ascribed to the foreign gene. Since the suitable host cell for the recombinant virus of the present invention is an avian cell which is available in a large quantity and not expensive, the recombinant virus of the present invention can be produced efficiently at low cost on a commercial scale.
摘要翻译：提供重组马立克氏病病毒，其包含减毒马立克氏病病毒的基因组DNA和来自另一来源的外源基因。本发明的重组病毒可有利地用作多功能活疫苗的活性成分，不仅具有马立克氏病病毒的抗原性和免疫原性，而且表现出归因于外源基因的性质。由于用于本发明的重组病毒的合适的宿主细胞是大量可用且不昂贵的禽细胞，所以可以以低成本有效地以商业规模生产本发明的重组病毒。

6. 发明授权

US5948411A Stabilized live vaccine 失效
标题翻译：稳定活疫苗
公开(公告)号：US5948411A
公开(公告)日：1999-09-07
申请号：US322036
申请日：1994-10-12
申请人： Kuniaki Koyama , Juichiro Osame
发明人： Kuniaki Koyama , Juichiro Osame
IPC分类号： A61K39/25 , A61K39/295 , A61P31/12 , A61P31/22 , A61K39/165 , A61K39/12 , C12N7/00 , C12N7/04
CPC分类号： A61K39/25 , A61K39/0015 , A61K39/12 , A61K2039/5254 , A61K2039/70 , C12N2710/16734
摘要： Disclosed is a stabilized live vaccine containing a varicella virus and a stabilizer, wherein the vaccine is substantially free of Ca.sup.2+ ions and Mg.sup.2+ ions. This stabilized live vaccine is extremely excellent in storage stability and heat resistance. Also disclosed is an improved stabilizer for a live varicella vaccine, comprising at least one member selected from gelatin and hydrolyzed gelatin, each being substantially free of Ca.sup.2+ ions and Mg.sup.2+ ions. The stabilizer can advantageously be used to stabilize a live vaccine containing a varicella virus. The substantial freedom of Ca.sup.2+ ions and Mg.sup.2+ ions can be attained by masking Ca.sup.2+ ions and Mg.sup.2+ ions present in a live vaccine containing a varicella virus and a stabilizer, with a chelating reagent, or by using as a stabilizer gelatin and/or a gelatin derivative after being purified to remove Ca.sup.2+ ions and/or Mg.sup.2+ ions contained therein.
摘要翻译：公开了含有水痘病毒和稳定剂的稳定的活疫苗，其中疫苗基本上不含Ca 2+离子和Mg 2+离子。这种稳定的活疫苗在储存稳定性和耐热性方面非常优异。还公开了一种用于活水痘疫苗的改进的稳定剂，其包含至少一种选自明胶和水解明胶的成分，各自基本上不含Ca 2+离子和Mg 2+离子。稳定剂可以有利地用于稳定含有水痘病毒的活疫苗。通过用螯合剂掩蔽含有水痘病毒和稳定剂的活疫苗中的Ca 2+离子和Mg 2+离子，或通过使用明胶和/或明胶衍生物作为稳定剂，可以获得Ca 2+离子和Mg 2+离子的实质自由度经纯化以除去其中所含的Ca 2+离子和/或Mg 2+离子。

7. 发明授权

US5693497A Process for expressing the hepatitis B virus antigen using a Saccharomyces cerevisiae transformant 失效
标题翻译：使用酿酒酵母转化体表达乙型肝炎病毒抗原的方法
公开(公告)号：US5693497A
公开(公告)日：1997-12-02
申请号：US378011
申请日：1995-01-25
申请人： Akihisa Takamizawa , Hiroyuki Fujita , Sadao Manabe , Masahiko Kato , Juichiro Osame , Iwao Yoshida , Takeo Konobe , Keisuke Takaku
发明人： Akihisa Takamizawa , Hiroyuki Fujita , Sadao Manabe , Masahiko Kato , Juichiro Osame , Iwao Yoshida , Takeo Konobe , Keisuke Takaku
IPC分类号： A61K39/29 , A61K39/00 , C07K14/00 , C07K14/02 , C07K14/10 , C07K14/18 , C12N15/00 , C12N15/09 , C12P21/02 , C12R1/865 , C12N1/19 , C12N15/51
CPC分类号： C07K14/005 , A61K39/00 , C12N2730/10122 , Y10S435/942
摘要： There is disclosed an antigen comprising an amino acid sequence of the suce antigen of a hepatitis B virus. The present antigen can be produced easily and safely at low cost by means of recombinant DNA technique. The present antigen can be used as an effective vaccine and diagnostic for hepatitis B.
摘要翻译：公开了包含乙型肝炎病毒的表面抗原的氨基酸序列的抗原。本发明的抗原可以通过重组DNA技术以低成本容易且安全地生产。本抗原可用作乙型肝炎的有效疫苗和诊断。

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