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1. 发明授权

US08138317B2 Purification of pegylated polypeptides 有权
标题翻译：聚乙二醇化多肽的纯化
公开(公告)号：US08138317B2
公开(公告)日：2012-03-20
申请号：US12218545
申请日：2008-07-16
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： C07K1/00 , B01D15/08
CPC分类号： C07K1/18 , A61K47/60 , C07K14/505
摘要： The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.
摘要翻译：本发明是使用两种阳离子交换层析步骤纯化单聚乙二醇化促红细胞生成素的方法，其中在阳离子交换色谱步骤中使用相同类型的阳离子交换材料，以及用于生产基本均匀形式的单PEG化促红细胞生成素的方法。

2. 发明授权

US06399333B1 Process for producing erythropoietin containing no animal proteins 失效
公开(公告)号：US06399333B1
公开(公告)日：2002-06-04
申请号：US08945802
申请日：1998-02-13
申请人： Josef Burg , Walter Schneider , Alexander Wrba , Werner Fürst , Karl-Heinz Sellinger
发明人： Josef Burg , Walter Schneider , Alexander Wrba , Werner Fürst , Karl-Heinz Sellinger
IPC分类号： C12P2104
CPC分类号： C07K14/505 , Y10S514/814
摘要： A process for producing erythropoietin which is free of foreign animal proteins except for the proteins of the host cell, wherein DNA coding for EPO is expressed in a eukaryotic host cell and the host cell is cultured in a medium free of natural mammalian proteins. The erythropoietin is chromatographically purified using dye affinity chromatography, chromatography on hydroxyapatite, reversed phase chromatography, and anion exchange chromatography. The resulting preparation contains less than 100 ppm of proteins derived from the host cell, and less than 10 pg of host cell DNA per 83 &mgr;g erythropoietin.

3. 发明申请

US20090118476A1 Purification of pegylated polypeptides 有权
标题翻译：聚乙二醇化多肽的纯化
公开(公告)号：US20090118476A1
公开(公告)日：2009-05-07
申请号：US12218545
申请日：2008-07-16
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： C07K1/18
CPC分类号： C07K1/18 , A61K47/60 , C07K14/505
摘要： The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.
摘要翻译：本发明是使用两种阳离子交换层析步骤纯化单聚乙二醇化促红细胞生成素的方法，其中在阳离子交换色谱步骤中使用相同类型的阳离子交换材料，以及用于生产基本均匀形式的单PEG化促红细胞生成素的方法。

4. 发明授权

US06471500B1 Process for producing erythropoietin containing no animal proteins 有权
公开(公告)号：US06471500B1
公开(公告)日：2002-10-29
申请号：US10067235
申请日：2002-02-07
申请人： Josef Burg , Walter Schneider , Alexander Wrba , Werner Fürst , Karl-Heinz Sellinger
发明人： Josef Burg , Walter Schneider , Alexander Wrba , Werner Fürst , Karl-Heinz Sellinger
IPC分类号： C12P1204
CPC分类号： C07K14/505 , Y10S514/814
摘要： A process for producing erythropoietin which is free of foreign animal proteins except for the proteins of the host cell, wherein DNA coding for EPO is expressed in a eukaryotic host cell and the host cell is cultured in a medium free of natural mammalian proteins. The erythropoietin is chromatographically purified using dye affinity chromatography, chromatography on hydroxyapatite, reversed phase chromatography, and anion exchange chromatography. The resulting preparation contains less than 100 ppm of proteins derived from the host cell, and less than 10 pg of host cell DNA per 83 &mgr;g erythropoietin.

5. 发明申请

US20120177640A1 OPTIMIZING THE PRODUCTION OF ANTIBODIES 审中-公开
标题翻译：优化抗体生产
公开(公告)号：US20120177640A1
公开(公告)日：2012-07-12
申请号：US13386175
申请日：2010-07-22
申请人： Josef Burg , Bernhard Hilger , Thorsten Kaiser , Wolfgang Kuhne , Lars Stiens , Claus Wallerius , Frank Zettl
发明人： Josef Burg , Bernhard Hilger , Thorsten Kaiser , Wolfgang Kuhne , Lars Stiens , Claus Wallerius , Frank Zettl
IPC分类号： A61K39/395 , A61P35/00
CPC分类号： C07K16/32 , C07K1/18 , C07K16/00 , C07K2317/10 , C07K2317/14 , C07K2317/24
摘要： A general method is provided for the production of purified antibodies by separation of an antibody molecule from an antibody variant by chromatographic methods, e.g. to enhance therapeutic efficacy, by for example choosing a specific harvesting time point and/or a specific purification scheme. The current invention thus reports a method for producing an antibody composition comprising an antibody molecule and a variant thereof, comprising the following steps: providing a sample comprising the antibody molecule and a variant thereof, determining the presence of the antibody molecule and/or a variant thereof and/or the ratio of the amount of the antibody molecule or variant thereof to the sum of the amounts of the antibody molecule and the variant thereof, in an aliquot of said sample, determining a subsequent harvesting time point and/or antibody purification scheme on basis of the data obtained before, thereby producing an antibody composition comprising the antibody molecule and a variant thereof.
摘要翻译：提供了通过色谱法分离抗体分子与抗体变体的纯化抗体的一般方法，例如，以通过例如选择特定的收获时间点和/或特定的纯化方案来增强治疗功效。因此，本发明报告了一种生产包含抗体分子及其变体的抗体组合物的方法，包括以下步骤：提供包含抗体分子及其变体的样品，确定抗体分子和/或变体的存在和/或所述样品的等分试样中抗体分子或其变体的量与抗体分子及其变体的量的总和的比例，确定随后的收获时间点和/或抗体纯化方案基于以前获得的数据，从而产生包含抗体分子及其变体的抗体组合物。

6. 发明申请

US20050288220A1 Erythropoietin with high specific activity 有权
标题翻译：具有高比活性的红细胞生成素
公开(公告)号：US20050288220A1
公开(公告)日：2005-12-29
申请号：US11201006
申请日：2005-08-09
申请人： Josef Burg , Karl-Heinz Sellinger , Anton Haselbeck , Hans Koll
发明人： Josef Burg , Karl-Heinz Sellinger , Anton Haselbeck , Hans Koll
IPC分类号： C12N15/09 , A61K38/00 , A61K38/18 , A61K38/22 , A61P7/06 , A61P43/00 , C07K1/20 , C07K1/22 , C07K14/505 , C12N5/06 , C12N5/08 , C12N5/10 , C12N15/16 , C12N15/85 , C12P21/02
CPC分类号： C07K14/505 , A61K38/00 , C07K2319/036 , C07K2319/75
摘要： The invention concerns new EPO compositions with high specific activity which are characterized by a high proportion of N-acetyl-lactosamine units or/and tetraantennary branches in the carbohydrate structure. In addition the invention concerns a process for producing such EPO products.
摘要翻译：本发明涉及具有高比活性的新的EPO组合物，其特征在于碳水化合物结构中N-乙酰 - 乳糖胺单元或/或四支链分支的高比例。此外，本发明涉及生产这种EPO产品的方法。

7. 发明授权

US5037764A Process for determination of an analyte and reagent therefor 失效
标题翻译：用于测定分析物和试剂的方法
公开(公告)号：US5037764A
公开(公告)日：1991-08-06
申请号：US73989
申请日：1987-07-16
申请人： Hans-Erich Wilk , Helmut Freitag , Josef Burg , Johann Berger
发明人： Hans-Erich Wilk , Helmut Freitag , Josef Burg , Johann Berger
IPC分类号： G01N33/58 , C12Q1/00 , G01N33/543 , G01N33/78
CPC分类号： G01N33/78 , G01N33/54306
摘要： The present invention provides a process for the detection of an analyte, wherein a sample containing the analyte is incubated either with a labelled binding partner and the immobilized analyte or an immobilized analyte analogue or with the labelled analyte or analyte analogue and an immobilized binding partner, the binding partner displaying towards the immobilized or labelled analyte or analyte analogue a higher affinity than towards the free analyte, and the labelled binding partner or the labelled analyte or the labelled analyte analogue is present in insufficiency compared with the concentration of the analyte to be detected.The present invention also provides an agent for carrying out this process, wherein it contains either the immobilized analyte or an immobilized analyte analogue and a labelled binding partner or the labelled analyte or a labelled analyte analogue and an immobilized binding partner, as well as optionally an appropriate buffer system and further conventionally used adjuvants, the binding partner displaying towards the immobilized or labelled analyte or analyte analogue a higher affinity than towards the free analyte.
摘要翻译：本发明提供了一种用于检测分析物的方法，其中将含有分析物的样品与标记的结合配偶体和固定的分析物或固定的分析物类似物或标记的分析物或分析物类似物和固定的结合配偶体一起温育，与被检测的分析物的浓度相比，向固定的或标记的分析物或分析物类似物的结合配体显示出比对于游离的分析物更高的亲合力，并且标记的结合配偶体或标记的分析物或标记的分析物类似物的存在不足。本发明还提供了用于进行该方法的试剂，其中其包含固定的分析物或固定的分析物类似物和标记的结合配偶体或标记的分析物或标记的分析物类似物和固定的结合配偶体，以及任选的合适的缓冲系统和进一步常规使用的佐剂，向固定的或标记的分析物或分析物类似物的结合配偶体显示比对游离的分析物更高的亲和力。

8. 发明授权

US08889837B2 Purification of pegylated polypeptides 有权
标题翻译：聚乙二醇化多肽的纯化
公开(公告)号：US08889837B2
公开(公告)日：2014-11-18
申请号：US12931070
申请日：2011-01-24
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： C07K1/14 , C07K1/16 , C07K1/18 , C07K1/22 , C07K14/505 , A61K47/48
CPC分类号： C07K1/18 , A61K47/60 , C07K14/505
摘要： The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.
摘要翻译：本发明是使用两种阳离子交换层析步骤纯化单聚乙二醇化促红细胞生成素的方法，其中在阳离子交换色谱步骤中使用相同类型的阳离子交换材料，以及用于生产基本均匀形式的单PEG化促红细胞生成素的方法。

9. 发明申请

US20110118448A1 Purification of pegylated polypeptides 有权
标题翻译：聚乙二醇化多肽的纯化
公开(公告)号：US20110118448A1
公开(公告)日：2011-05-19
申请号：US12931070
申请日：2011-01-24
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： C07K14/575
CPC分类号： C07K1/18 , A61K47/60 , C07K14/505
摘要： The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.
摘要翻译：本发明是使用两种阳离子交换层析步骤纯化单聚乙二醇化促红细胞生成素的方法，其中在阳离子交换色谱步骤中使用相同类型的阳离子交换材料，以及用于生产基本均匀形式的单PEG化促红细胞生成素的方法。

10. 发明授权

US07846336B2 Chromatographic methods 有权
标题翻译：色谱法
公开(公告)号：US07846336B2
公开(公告)日：2010-12-07
申请号：US12218543
申请日：2008-07-16
申请人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
发明人： Josef Burg , Klaus Reichert , Axel Schroth , Hartmut Schurig , Axel Wessner
IPC分类号： B01D15/08
CPC分类号： B01D15/203 , B01D15/362 , B01J49/53 , B01J49/60 , C07K1/18 , C07K14/505
摘要： The current invention comprises a method for the regeneration of a cation exchange chromatography column.
摘要翻译：本发明包括用于再生阳离子交换色谱柱的方法。

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