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1. 发明申请

US20070254371A1 Peptide combos and their uses 审中-公开
标题翻译：肽组合及其用途
公开(公告)号：US20070254371A1
公开(公告)日：2007-11-01
申请号：US11305737
申请日：2005-12-16
申请人： Koen Kas , Joel Vandekerckhove , Luc Krols
发明人： Koen Kas , Joel Vandekerckhove , Luc Krols
IPC分类号： G01N33/00 , C07K14/00 , C12N9/14
CPC分类号： G01N33/6803 , C07K14/47 , G01N33/6806 , G01N2333/726 , G01N2800/52
摘要： The invention provides reagents and methods for the accurate quantification of proteins in complex biological samples. Quantification is obtained by adding to a sample a peptide combo, which is essentially a collection of synthetic reference peptides. The synthetic reference peptides have a small mass difference when compared to the biological reference peptides that originate upon digestion from the proteins present in the sample. Reference peptides and synthetic reference peptides are selected and the identity and accurate amounts of reference peptides are determined by mass spectrometry. The methods can be used in high throughput assays to interrogate proteomes.
摘要翻译：本发明提供用于准确定量复杂生物样品中蛋白质的试剂和方法。通过向样品中加入基本上是合成参考肽的集合的肽组合物来获得定量。当与从样品中存在的蛋白质消化而产生的生物参考肽相比时，合成参考肽具有小的质量差异。选择参考肽和合成参考肽，并通过质谱法测定参考肽的同一性和准确量。该方法可用于高通量测定以询问蛋白质组。

2. 发明申请

US20050196823A1 Methods and apparatus for gel-free qualitative and quantitative proteome analysis, and uses therefore 审中-公开
公开(公告)号：US20050196823A1
公开(公告)日：2005-09-08
申请号：US10952557
申请日：2004-09-28
申请人： Joel Vandekerckhove , Kris Gevaert
发明人： Joel Vandekerckhove , Kris Gevaert
IPC分类号： G01N27/62 , C07K1/16 , C07K1/20 , C07K1/36 , C12P21/02 , G01N30/02 , G01N30/06 , G01N30/34 , G01N30/46 , G01N30/72 , G01N30/80 , G01N30/84 , G01N30/88 , G01N33/48 , G01N33/50 , G01N33/68 , C12Q1/37 , G01N33/00
CPC分类号： G01N30/461 , B01D15/1871 , C07K1/36 , G01N30/467 , G01N30/468 , G01N30/7233 , G01N30/80 , G01N33/6842 , G01N33/6848 , G01N2030/8411 , Y10S435/803
摘要： Methods and apparatus for qualitative and quantitative proteome analysis are provided. The methods and apparatus allow for the isolation of a subset of peptides out of complex mixtures of peptides. The isolation is based on a specific chemical and/or enzymatic alteration of one or more types of peptides. This alteration modifies the biophysical, chemical or any other biochemical property of the affected types of peptides (e.g., net electrical charge and/or hydrophobicity) in such way that the altered peptides can be separated from the unaltered peptides.

3. 发明申请

US20060160131A1 Method for the identification of drug targets 审中-公开
标题翻译：药物靶标鉴定方法
公开(公告)号：US20060160131A1
公开(公告)日：2006-07-20
申请号：US10527662
申请日：2003-09-11
申请人： Joel Vandekerckhove , Kris Gevaert
发明人： Joel Vandekerckhove , Kris Gevaert
IPC分类号： G01N33/53 , C12Q1/37
CPC分类号： C40B30/04
摘要： The present invention relates to the field of drug development. More specifically the invention provides a method for the identification of drug targets. The method can also be used for analysis of proteomes. The method utilizes in essence a combination of two chromatographic separations of the same type, separated by a step in which the population of the drug-bound targets is altered specifically on the drug in such a way that the chromatographic behaviour of the altered drug-bound targets in the second chromatographic separation differs from the chromatographic behaviour of its unaltered version. The different chromatographic behaviour of the altered drug-bound targets is used for the isolation and subsequent identification of the targets.
摘要翻译：本发明涉及药物开发领域。更具体地，本发明提供了用于鉴定药物靶标的方法。该方法也可用于蛋白质组学的分析。该方法本质上是使用相同类型的两个色谱分离的组合，通过药物结合靶标的群体在药物上特异性改变的步骤分开，使得改变的药物结合的色谱行为第二个色谱分离中的目标不同于其未改变版本的色谱行为。改变的药物结合靶标的不同色谱行为用于分离和随后鉴定靶标。

4. 发明授权

US06908740B2 Methods and apparatus for gel-free qualitative and quantitative proteome analysis, and uses therefore 有权
标题翻译：用于无凝胶定性和定量蛋白质组分析的方法和装置，因此使用
公开(公告)号：US06908740B2
公开(公告)日：2005-06-21
申请号：US10394980
申请日：2003-03-21
申请人： Joel Vandekerckhove , Kris Gevaert
发明人： Joel Vandekerckhove , Kris Gevaert
IPC分类号： G01N27/62 , C07K1/16 , C07K1/20 , C07K1/36 , C12P21/02 , G01N30/02 , G01N30/06 , G01N30/34 , G01N30/46 , G01N30/72 , G01N30/80 , G01N30/84 , G01N30/88 , G01N33/48 , G01N33/50 , G01N33/68 , G01N33/53 , A23J1/00 , C07K1/00 , C12Q1/00 , C12Q1/37
CPC分类号： G01N30/461 , B01D15/1871 , C07K1/36 , G01N30/467 , G01N30/468 , G01N30/7233 , G01N30/80 , G01N33/6842 , G01N33/6848 , G01N2030/8411 , Y10S435/803
摘要： Methods and apparatus for qualitative and quantitative proteome analysis are provided. The methods and apparatus allow for the isolation of a subset of peptides out of complex mixtures of peptides. The isolation is based on a specific chemical and/or enzymatic alteration of one or more types of peptides. This alteration modifies the biophysical, chemical or any other biochemical property of the affected types of peptides (e.g., net electrical charge and/or hydrophobicity) in such way that the altered peptides can be separated from the unaltered peptides.
摘要翻译：提供了定性和定量蛋白质组分析的方法和装置。所述方法和装置允许从肽的复杂混合物中分离肽的子集。分离基于一种或多种类型的肽的特定化学和/或酶促改变。这种改变改变受影响类型的肽的生物物理，化学或任何其它生物化学性质（例如，净电荷和/或疏水性），使得改变的肽可以与未改变的肽分离。

5. 发明授权

US5589615A Process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins 失效
标题翻译：通过表达改性的2S储存蛋白来生产具有增加的营养价值的转基因植物的方法
公开(公告)号：US5589615A
公开(公告)日：1996-12-31
申请号：US229069
申请日：1994-04-18
申请人： Ann De Clercq , Enno Krebbers , Joel Vandekerckhove , Luiz Barreto De Castro , Eugen Gander , Marc Van Montagu
发明人： Ann De Clercq , Enno Krebbers , Joel Vandekerckhove , Luiz Barreto De Castro , Eugen Gander , Marc Van Montagu
IPC分类号： C07K14/00 , C07K14/415 , C07K14/60 , C07K14/70 , C12N15/82 , A01H4/00
CPC分类号： C07K14/415 , C07K14/00 , C07K14/60 , C07K14/70 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C07K2319/02
摘要： A process for the production of plants with increased content of appropriate amino acids having high nutritional properties through the modification of plant genes encoding plant storage proteins, particularly the 2S albumins. Seed-forming plants susceptible to Agrobacterium transformation, the genome of which plants includes a recombinant DNA encoding a precursor of a modified 2S albumin, under the control of a promoter, are described, including the particular recombinant DNA.
摘要翻译：通过修饰编码植物贮藏蛋白质的植物基因，特别是2S白蛋白来生产具有高营养特性的合适氨基酸含量增加的植物的方法。描述了对土壤杆菌转化敏感的成芽植物，其基因组包括编码改性的2S白蛋白的前体的重组DNA，其在启动子的控制下，包括特定的重组DNA。

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