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1. 发明申请

US20110078826A1 YIELD ENHANCEMENT IN PLANTS BY MODULATION OF A ZM-EREF-TS PROTEIN 审中-公开
标题翻译：通过调节ZM-EREF-TS蛋白质在植物中的增产
公开(公告)号：US20110078826A1
公开(公告)日：2011-03-31
申请号：US12891860
申请日：2010-09-28
申请人： Guofu Li , Jijun Zou
发明人： Guofu Li , Jijun Zou
IPC分类号： C12N15/82 , C07H21/04 , C07K14/415 , C12N15/63 , A01H5/00
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： Compositions and methods for modulating plant development and for increasing yield in a plant are provided. The compositions include a Zm-EREF-Ts sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1 and 2 as well as variants and fragments thereof. Nucleotide sequences encoding the Zm-EREF-Ts molecule are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of a Zm-EREF-Ts sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a Zm-EREF-Ts sequence of the invention. The level of the Zm-EREF-Ts polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.
摘要翻译：提供了调节植物发育和提高植物产量的组合物和方法。组合物包括Zm-EREF-Ts序列。本发明的组合物包含选自SEQ ID NO：1和2的氨基酸序列和核苷酸序列以及其变体和片段。在DNA构建体中提供编码Zm-EREF-Ts分子的核苷酸序列用于在感兴趣的植物中表达以提供用于调节植物或植物部分中的Zm-EREF-Ts序列的水平。所述方法包括向植物或植物部分引入包含本发明的Zm-EREF-Ts序列的异源多核苷酸。可以增加或减少Zm-EREF-Ts多肽的水平。这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。

2. 发明申请

US20140315201A1 METHOD FOR HIGH-THROUGHPUT SCREENING OF TRANSGENIC PLANTS 审中-公开
标题翻译：转基因植物高通量筛选方法
公开(公告)号：US20140315201A1
公开(公告)日：2014-10-23
申请号：US14236461
申请日：2012-07-23
申请人： Igor Cunha De Oliveira , Dennis O'Neill , Andrew Slattery , Jijun Zou
发明人： Igor Cunha De Oliveira , Dennis O'Neill , Andrew Slattery , Jijun Zou
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , A01H1/04 , C12N15/8216 , C12N15/8218 , C12Q2600/13
摘要： The invention relates to a method for quantifying levels of expression and/or quantifying copy number of a heterologous polynucleotide in a transgenic plant using quantitative or real-time polymerase chain reaction (QPCR or real-time PCR), wherein the real-time PCR is performed using a primer set specific to a heterologous terminator sequence operably linked to the heterologous polynucleotide.
摘要翻译：本发明涉及使用定量或实时聚合酶链反应（QPCR或实时PCR）定量转基因植物中异源多核苷酸的表达水平和/或定量拷贝数的方法，其中实时PCR是使用与异源多核苷酸可操作地连接的异源终止子序列特异的引物组来进行。

3. 发明授权

US08334426B1 Method for obtaining transformed maize plants 失效
标题翻译：获得转化玉米植物的方法
公开(公告)号：US08334426B1
公开(公告)日：2012-12-18
申请号：US12478008
申请日：2009-06-04
申请人： Yinghong Li , Jijun Zou , Igor C. Oliveira
发明人： Yinghong Li , Jijun Zou , Igor C. Oliveira
IPC分类号： C12N15/82
CPC分类号： C12N15/8205
摘要： Methods are provided for transforming isolated, immature maize embryos and for producing transgenic maize plants. The methods additionally comprise identifying or selecting transformed cells and regenerating such cells into transformed maize plants.
摘要翻译：提供了用于转化分离的，未成熟的玉米胚胎和用于产生转基因玉米植物的方法。所述方法另外包括鉴定或选择转化的细胞并将此类细胞再生成转化的玉米植物。

4. 发明申请

US20160002648A1 GENES FOR IMPROVING NUTRIENT UPTAKE AND ABIOTIC STRESS TOLERANCE IN PLANTS 审中-公开
标题翻译：改善植物营养摄取和生物胁迫耐受性的基因
公开(公告)号：US20160002648A1
公开(公告)日：2016-01-07
申请号：US14771528
申请日：2014-03-03
申请人： Mei GUO , Kevin R. HAYES , Brooke PETERSON-BURCH , Carl R. SIMMONS , Shoba SIVASANKAR , Jijun ZOU , PIONEER HI-BRED INTERNATIONAL INC.
发明人： MEI GUO , KEVIN HAYES , BROOKE PETERSON-BURCH , CARL SIMMONS , SOBHANA SIVASANKAR , JIJUN ZOU
IPC分类号： C12N15/82 , C12N9/12 , C12Q1/68 , C07K14/415
CPC分类号： C12N15/8243 , C07K14/415 , C12N9/12 , C12N15/8218 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8286 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Y207/11025 , Y02A40/146
摘要： The present disclosure provides methods to increase crop yield utilizing transgenic genes controlling plant growth and yield. The specific genes can be used to increase tissue growth and abiotic stress tolerance. Plants, plant progeny, seeds and tissues created by these methods are also described. Polynucleotides encoding the sequences are provided for expression in a plant of interest. Expression cassettes, plants, plant cells, plant parts and seeds comprising the sequences of the disclosure are further provided. In specific embodiments, the polynucleotide is operably linked to a constitutive promoter.
摘要翻译：本公开提供了利用控制植物生长和产量的转基因基因提高作物产量的方法。具体的基因可用于增加组织生长和非生物胁迫耐受性。还描述了通过这些方法产生的植物，植物后代，种子和组织。提供编码序列的多核苷酸用于在感兴趣的植物中表达。还提供了包含本公开的序列的表达盒，植物，植物细胞，植物部分和种子。在具体实施方案中，多核苷酸可操作地连接于组成型启动子。

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