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1. 发明授权

US5395588A Control of flow cytometer having vacuum fluidics 失效
标题翻译：具有真空流体的流式细胞仪的控制
公开(公告)号：US5395588A
公开(公告)日：1995-03-07
申请号：US19716
申请日：1993-02-19
申请人： Howard North, Jr. , Kenneth F. Uffenheimer
发明人： Howard North, Jr. , Kenneth F. Uffenheimer
IPC分类号： G01N15/14 , G01N33/49 , G01N21/11 , G01N27/08
CPC分类号： G01N15/1404
摘要： The present invention relates to a system for controlling the movement or transport of objects such as cells and other particles in a moving liquid stream. More particularly the invention relates to a system including a vacuum pump which pulls the sheath fluid from an open supply reservoir through a flow cell assembly where cell analysis occurs and discharges the flow cell effluent to an open waste reservoir. A pressure drop is created through the conduit leading from the supply reservoir to the flow cell which aspirates the cell suspension from an open sample vessel into and through the flow cell. The vacuum is regulated by controlling the electric power applied to the vacuum pump motor. Starting, stopping, reverse flushing, and draining of the flow cell assembly are controlled by programming the operation of four electric solenoid valves in fluid communication with the flow cell inlet and outlet passages, the operation of a reverse flush pump and, the operation of an electrically controlled tube lifter. A deaerator is provided to remove some of the air dissolved in the sheath fluid provided to the flow cell assembly to prevent the release of air and formation of adherent air bubbles in sensitive areas of the flow cell assembly.
摘要翻译：本发明涉及一种用于控制移动液体流中诸如细胞和其它颗粒的物体的运动或运输的系统。更具体地，本发明涉及一种包括真空泵的系统，该真空泵通过流动池组件从开放的供应储存器拉动护套流体，其中进行细胞分析并将流动池流出物排出到开放的废物储存器。通过从供应储存器引导到流动池的导管产生压降，该流动池将细胞悬浮液从开放的样品容器吸入并通过流动池。通过控制施加到真空泵马达的电力来调节真空。通过编程与流动池入口和出口通道流体连通的四个电磁阀的操作，反冲洗泵的操作以及操作一个或多个流量单元的操作来控制流动池组件的启动，停止，反向冲洗和排水电控管升降器提供除气器以去除溶于提供给流动池组件的护套流体中的一些空气，以防止空气的释放和在流动池组件的敏感区域中粘附的气泡的形成。

2. 发明授权

US3931018A Assembly for collection, separation and filtration of blood 失效
标题翻译：汇集，分离和过滤血液
公开(公告)号：US3931018A
公开(公告)日：1976-01-06
申请号：US496029
申请日：1974-08-09
申请人： Howard North, Jr.
发明人： Howard North, Jr.
IPC分类号： A61B5/15 , B01D33/01 , B01L3/14 , B01L99/00 , G01N33/48 , G01N33/49 , B01D33/00
CPC分类号： G01N33/491 , B01D33/01 , B01L3/50215
摘要： Devices are disclosed which are useful in the simultaneous separation and filtration of the liquid portion of clotted whole blood and anti-coagulant treated whole blood. The devices are employed in novel apparatus of the invention, for the collection, separation and filtration of blood serum and blood plasma from clotted whole blood and anti-coagulant treated whole blood, respectively.

3. 发明授权

US4844610A Backflow isolator and capture system 失效
标题翻译：回流隔离器和捕获系统
公开(公告)号：US4844610A
公开(公告)日：1989-07-04
申请号：US188582
申请日：1988-04-29
申请人： Howard North, Jr.
发明人： Howard North, Jr.
IPC分类号： G01N33/49 , B01L99/00 , G01N15/14
CPC分类号： G01N15/1404 , G01N2015/1409
摘要： A flow apparatus for the analysis of particles passing with a liquid substantially one at a time through an analysis region includes a backflow isolator and capture system to catch any liquid and particles dripping from the analysis region. The backflow isolator and capture system is activated by an unlocking support for the sample test tube carrying the supply of liquid and particles. The support is also an operator for a pump and a pressure vent valve whereby liquid, particles and aerosols cannot escape. A method for isolating and capturing backflow of a supply of particles to be analyzed includes steps of venting the air supply, moving a test tube support and activating a pump.

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