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1. 发明授权

US5492810A DNA silver staining 失效
标题翻译： DNA银染
公开(公告)号：US5492810A
公开(公告)日：1996-02-20
申请号：US193514
申请日：1994-02-08
申请人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
发明人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
IPC分类号： G01N27/447 , C12Q1/68 , G01N27/26
CPC分类号： G01N27/44726
摘要： A method for visualizing nucleic acids in a polyacrylamide gel. The method comprises fixing the nucleic acids with 10% acetic acid for about 20 minutes, washing the gel multiple times with water for about 2 minutes, impregnating the gel with silver nitrate at a concentration of about 1.0 g/l and about 1.5 ml/l of 37% formaldehyde for about 30 minutes, developing the gel with sodium carbonate at a concentration of about 30 g/l, 1.5 ml/l of 37% formaldehyde and sodium thiosulfate pentahydrate at a concentration of about 2.0 mg/l for between about 2 minutes and about 5 minutes, and then stopping the development of the gel by treatment with 10% acetic acid for about 5 minutes.
摘要翻译：一种在聚丙烯酰胺凝胶中可视化核酸的方法。该方法包括用10％乙酸固定核酸约20分钟，用水洗涤凝胶多次约2分钟，用浓度约1.0g / l和约1.5ml / l的硝酸银浸渍凝胶的37％甲醛约30分钟，用约30g / l的浓度的碳酸钠，1.5ml / l的37％甲醛和硫代硫酸钠五水合物以约2.0mg / l的浓度显影凝胶约2 分钟和约5分钟，然后用10％乙酸处理约5分钟停止凝胶的显影。

2. 发明授权

US5413909A Method for profiling nucleic acids of unknown sequence using arbitrary oligonucleotide primers 失效
标题翻译：使用任意寡核苷酸引物分析未知序列核酸的方法
公开(公告)号：US5413909A
公开(公告)日：1995-05-09
申请号：US6380
申请日：1993-01-19
申请人： Brant J. Bassam , Gustavo Caetano-Anolles , Peter M. Gresshoff
发明人： Brant J. Bassam , Gustavo Caetano-Anolles , Peter M. Gresshoff
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6858 , C12N15/10 , C12Q1/686
摘要： A method for amplifying nucleic acid sequences in a template comprising at least one nucleic acid or mixture of nucleic acids wherein each nucleic acid comprises at least one strand. The method comprises the steps of treating the template with at least one oligonucleotide primer having a nucleic acid sequence that is substantially complementary to at least one nucleic acid sequence on the template under conditions such that the primer will bind to a segment that is substantially complementary to the primer, producing primed strands of nucleic acid. The primed strands produce extension strands, wherein the extension strands comprise the primer in combination with a sequence of nucleic acids that is substantially complementary to the template in a 5' to 3' direction from the primer. The extension strands are further treated with the primer producing amplification strands which are treated with primer. The amplification strands are repeatedly treated with primer to produce large numbers of amplification strands whereby the nucleic acid sequences of the amplification strands are substantially similar to nucleic acid sequences in the original template.
摘要翻译：一种用于扩增模板中的核酸序列的方法，其包含至少一种核酸或核酸混合物，其中每个核酸包含至少一个链。该方法包括以下步骤：用至少一种寡核苷酸引物处理模板，所述寡核苷酸引物具有与模板上至少一个核酸序列基本上互补的条件，使得引物将结合至基本上与引物，产生引发的核酸链。引发的链产生延伸链，其中延伸链包含与来自引物的5'至3'方向上的模板基本上互补的核酸序列的引物。延伸链进一步用引物产生的引物处理的引物进行处理。用引物重复处理扩增链以产生大量的扩增链，由此扩增链的核酸序列基本上与原始模板中的核酸序列相似。

3. 发明授权

US5962221A Oligonucleotide constructs and methods for the generation of sequence signatures from nucleic acids 失效
标题翻译：用于从核酸产生序列特征的寡核苷酸构建体和方法
公开(公告)号：US5962221A
公开(公告)日：1999-10-05
申请号：US489269
申请日：1995-06-09
申请人： Gustavo Caetano-Anolles
发明人： Gustavo Caetano-Anolles
IPC分类号： C12Q1/68 , C07K21/04
CPC分类号： C12Q1/6876 , C12Q1/6827 , C12Q1/6837 , C12Q1/6858 , C12Q1/686 , C12Q2600/166 , Y10S435/81
摘要： Novel oligonucleotides for amplification and profiling of nucleic acid templates are disclosed. Enhancements of nucleic acid fingerprinting methods are disclosed.
摘要翻译：公开了用于扩增和分析核酸模板的新型寡核苷酸。公开了核酸指纹方法的增强。

4. 发明授权

US6074818A Fingerprinting of nucleic acids, products and methods 失效
标题翻译：指纹核酸，产品和方法
公开(公告)号：US6074818A
公开(公告)日：2000-06-13
申请号：US139459
申请日：1993-10-20
申请人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
发明人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
IPC分类号： G01N33/50 , C07H21/00 , C07H21/04 , C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6853 , Y10S435/81
摘要： Improvements to nucleic acid fingerprinting are disclosed. The method generates profiles of increased fidelity characteristic of the nucleic acids analyzed. Novel primers and other means are taught. Useful products are disclosed.
摘要翻译：公开了对核酸指纹图谱的改进。该方法产生所分析的核酸的增强的保真特征的分布。教导了新型底漆和其他方法。有用的产品被公开。

5. 发明授权

US5567585A Method and kit for silver staining, developing an image and visualizing biological materials 失效
标题翻译：用于银染的方法和试剂盒，开发图像和可视化生物材料
公开(公告)号：US5567585A
公开(公告)日：1996-10-22
申请号：US258553
申请日：1994-06-09
申请人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
发明人： Gustavo Caetano-Anolles , Brant J. Bassam , Peter M. Gresshoff
IPC分类号： C12Q1/68 , G01N27/447 , G01N27/26
CPC分类号： G01N27/44726 , C12Q1/6816
摘要： A silver staining method is provided for staining an organic molecule capable of binding silver. An improved image is developed and visualized. A kit useful in practicing the method is described. A permanent record of the image of the profile of the stained molecules is obtained.
摘要翻译：提供银染色方法来染色能够结合银的有机分子。改进的图像被开发和可视化。描述了在实践该方法中有用的试剂盒。获得染色分子轮廓图像的永久记录。

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