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    • 10. 发明授权
    • Transfection with micro explosion enacted by coated dry ice particles
    • 用涂覆的干冰颗粒进行微量爆炸的转染
    • US08415160B2
    • 2013-04-09
    • US12957035
    • 2010-11-30
    • Fu-Jen KaoYung-En Kuo
    • Fu-Jen KaoYung-En Kuo
    • C12N15/87
    • C12N15/87C12N15/88
    • Disclosed is a transfection method, which includes the steps of: (a) adhering the gene fragments to dry ice particles; (b) adding the dry ice particles into the medium/liquid that contains target cells; and (c) transporting the gene fragments into the target cells via the micro explosion/sublimation of the dry ice particles. In addition, the gene fragments can also adhere first to nanoparticles, which can then adhere to dry ice particles. Subsequently, gene fragments enter cells by micro explosion/sublimation. The present invention can be applied in transgenic research on prokaryotic, eukaryotic, plant and animal cells and in the development of new species in agriculture.
    • 公开了一种转染方法,其包括以下步骤:(a)将基因片段粘附到干冰颗粒上; (b)将干冰颗粒加入到含有靶细胞的介质/液体中; 和(c)通过干冰颗粒的微爆/升华将基因片段运送到靶细胞中。 此外,基因片段还可以首先粘附到纳米颗粒上,然后可以将其粘附到干冰颗粒上。 随后,基因片段通过微量爆炸/升华进入细胞。 本发明可应用于原核,真核,植物和动物细胞的转基因研究以及农业新品种的开发。