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1. 发明授权

US06579459B2 System and method for performing polynucleotide separations using liquid chromatography 失效
公开(公告)号：US06579459B2
公开(公告)日：2003-06-17
申请号：US10086301
申请日：2002-02-28
申请人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
发明人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
IPC分类号： B01D1508
CPC分类号： C12N15/101 , B01D15/125 , B01D15/366 , B01J45/00 , G01N30/14 , G01N2030/085 , G01N2030/528 , G01N30/6039
摘要： Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.

2. 发明授权

US06174441B1 Method for performing polynucleotide separations using liquid chromatography 失效
标题翻译：使用液相色谱进行多核苷酸分离的方法
公开(公告)号：US06174441B1
公开(公告)日：2001-01-16
申请号：US09413076
申请日：1999-10-05
申请人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
发明人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
IPC分类号： B01D1508
CPC分类号： C12N15/101 , B01D15/12 , B01D15/366 , B01D15/38 , B01J45/00 , G01N30/14 , G01N2030/528
摘要： Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.
摘要翻译：具有由钛，不锈钢或有机聚合材料制成的组分的改进的液相色谱系统可用于通过匹配离子多核苷酸色谱（MIPC）分离多核苷酸片段，特别是双链多核苷酸的大片段。处理钛，不锈钢或聚合物组分，使得它们不会将多价阳离子释放到流过色谱系统的水溶液中。或者除了利用由上述组分制成的材料之外，还可以使用放置在分离塔上游的多价阳离子捕获树脂从体系中除去多价离子。多价阳离子捕获树脂可以包含在保护盘，保护柱或防护盒中。基于其分子量通过匹配离子多核苷酸色谱法和回旋分离色谱法将多核苷酸片段的混合物分离成级分的新方法使用上述液相色谱系统。

3. 发明授权

US06491821B2 System and method for performing polynucleotide separations using liquid chromatography 失效
标题翻译：使用液相色谱进行多核苷酸分离的系统和方法
公开(公告)号：US06491821B2
公开(公告)日：2002-12-10
申请号：US09828346
申请日：2001-04-05
申请人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
发明人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
IPC分类号： B01D1508
CPC分类号： B01D15/366 , C12N15/101
摘要： Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.
摘要翻译：具有由钛，不锈钢或有机聚合材料制成的组分的改进的液相色谱系统可用于通过匹配离子多核苷酸色谱（MIPC）分离多核苷酸片段，特别是双链多核苷酸的大片段。处理钛，不锈钢或聚合物组分，使得它们不会将多价阳离子释放到流过色谱系统的水溶液中。或者除了利用由上述组分制成的材料之外，还可以使用放置在分离塔上游的多价阳离子捕获树脂从体系中除去多价离子。多价阳离子捕获树脂可以包含在保护盘，保护柱或防护盒中。基于其分子量通过匹配离子多核苷酸色谱法和回旋分离色谱法将多核苷酸片段的混合物分离成级分的新方法使用上述液相色谱系统。

4. 发明授权

US06251272B1 System and column for performing polynucleotide separations using liquid chromatography 失效
标题翻译：使用液相色谱进行多核苷酸分离的系统和色谱柱
公开(公告)号：US06251272B1
公开(公告)日：2001-06-26
申请号：US09350774
申请日：1999-07-09
申请人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
发明人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
IPC分类号： B01D1508
CPC分类号： C12N15/101 , B01D15/12 , B01D15/366 , B01D15/38 , B01J45/00 , G01N30/14 , G01N2030/528
摘要： Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.
摘要翻译：具有由钛，不锈钢或有机聚合材料制成的组分的改进的液相色谱系统可用于通过匹配离子多核苷酸色谱（MIPC）分离多核苷酸片段，特别是双链多核苷酸的大片段。处理钛，不锈钢或聚合物组分，使得它们不会将多价阳离子释放到流过色谱系统的水溶液中。或者除了利用由上述组分制成的材料之外，还可以使用放置在分离塔上游的多价阳离子捕获树脂从体系中除去多价离子。多价阳离子捕获树脂可以包含在保护盘，保护柱或防护盒中。基于其分子量通过匹配离子多核苷酸色谱法和回旋分离色谱法将多核苷酸片段的混合物分离成级分的新方法使用上述液相色谱系统。

5. 发明授权

US6156206A Process for performing polynucleotide separations 失效
公开(公告)号：US6156206A
公开(公告)日：2000-12-05
申请号：US324350
申请日：1999-06-02
申请人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
发明人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
IPC分类号： B01D15/08 , B01D15/12 , B01D15/36 , B01D15/38 , C12N15/10 , G01N30/14 , G01N30/52
CPC分类号： B01J45/00 , B01D15/12 , B01D15/366 , B01D15/38 , C12N15/101 , G01N2030/528 , G01N30/14
摘要： The invention recognizes the deleterious effects of trace, and even undetectable amounts of multivalent cations on the separation of mixtures of polynucleotides, especially double stranded polynucleotides, and provides an improved method for separating such mixtures on wide pore, non-polar separation media by eliminating multivalent cations from the all aspects of the separation process. This is accomplished by using components in the separation process which are materials which do not release metal cations. In addition, the use of cation capture resins and other methods to remove residual traces of multivalent cations from eluting solvents, sample solutions, separation media, and system components is described. It is also important to remove any traces or organic contaminants from solvents solutions and system parts. Taking similar steps to remove residual traces of multivalent cations and organic impurities from the separation process, the invention may also be used in a batch process to separate mixtures of polynucleotide fragments.

6. 发明授权

US5997742A Method for performing polynucleotide separations using liquid chromatography 失效
公开(公告)号：US5997742A
公开(公告)日：1999-12-07
申请号：US81040
申请日：1998-05-18
申请人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
发明人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
IPC分类号： B01D15/08 , B01D15/12 , B01D15/36 , B01D15/38 , B01J45/00 , C12N15/10 , G01N30/14 , G01N30/52
CPC分类号： C12N15/101 , B01D15/12 , B01D15/366 , B01D15/38 , B01J45/00 , G01N2030/528 , G01N30/14
摘要： Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.

7. 发明授权

US6030527A Apparatus for performing polynucleotide separations using liquid chromatography 有权
标题翻译：使用液相色谱进行多核苷酸分离的装置
公开(公告)号：US6030527A
公开(公告)日：2000-02-29
申请号：US350737
申请日：1999-07-09
申请人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
发明人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
IPC分类号： B01D15/08
CPC分类号： B01D15/08
摘要： Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.
摘要翻译：具有由钛，不锈钢或有机聚合材料制成的组分的改进的液相色谱系统可用于通过匹配离子多核苷酸色谱（MIPC）分离多核苷酸片段，特别是双链多核苷酸的大片段。处理钛，不锈钢或聚合物组分，使得它们不会将多价阳离子释放到流过色谱系统的水溶液中。或者除了利用由上述组分制成的材料之外，还可以使用放置在分离塔上游的多价阳离子捕获树脂从体系中除去多价离子。多价阳离子捕获树脂可以包含在保护盘，保护柱或防护盒中。基于其分子量通过匹配离子多核苷酸色谱法和回旋分离色谱法将多核苷酸片段的混合物分离成级分的新方法使用上述液相色谱系统。

8. 发明授权

US6017457A Method for performing polynucleotide separations using liquid chromatography 失效
标题翻译：使用液相色谱进行多核苷酸分离的方法
公开(公告)号：US6017457A
公开(公告)日：2000-01-25
申请号：US80547
申请日：1998-05-18
申请人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
发明人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
IPC分类号： B01D15/08 , B01D15/12 , B01D15/36 , B01D15/38 , B01J45/00 , C12N15/10 , G01N30/14 , G01N30/52
CPC分类号： C12N15/101 , B01D15/12 , B01D15/366 , B01D15/38 , B01J45/00 , G01N2030/528 , G01N30/14
摘要： Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.
摘要翻译：具有由钛，不锈钢或有机聚合材料制成的组分的改进的液相色谱系统可用于通过匹配离子多核苷酸色谱（MIPC）分离多核苷酸片段，特别是双链多核苷酸的大片段。处理钛，不锈钢或聚合物组分，使得它们不会将多价阳离子释放到流过色谱系统的水溶液中。或者除了利用由上述组分制成的材料之外，还可以使用放置在分离塔上游的多价阳离子捕获树脂从体系中除去多价离子。多价阳离子捕获树脂可以包含在保护盘，保护柱或防护盒中。基于其分子量通过匹配离子多核苷酸色谱法和回旋分离色谱法将多核苷酸片段的混合物分离成级分的新方法使用上述液相色谱系统。

9. 发明授权

US5772889A System and method for performing nucleic acid separations using liquid chromatography 失效
标题翻译：使用液相色谱进行核酸分离的系统和方法
公开(公告)号：US5772889A
公开(公告)日：1998-06-30
申请号：US748376
申请日：1996-11-13
申请人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
发明人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
IPC分类号： B01D15/12 , B01D15/36 , B01D15/38 , B01J45/00 , C12N15/10 , G01N30/14 , G01N30/52 , B01D15/08
CPC分类号： B01D15/366 , B01D15/12 , B01D15/38 , B01J45/00 , C12N15/101 , G01N2030/528 , G01N30/14
摘要： Improved liquid chromatography systems having components made of titanium, coated stainless steel, or organic polymeric material are useful in the separation of nucleic acid fragments, particularly large fragments of double-stranded nucleic acids, by ion pairing reverse phase chromatography. The titanium, coated stainless steel, or polymeric components do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column may be employed to remove multivalent ions from the system. The multivalent cation capture resin may be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of nucleic acid fragments into fractions based on their molecular weight by ion pairing reverse phase chromatography and slalom chromatography utilize the liquid chromatographic systems described above.
摘要翻译：具有由钛，涂层不锈钢或有机聚合材料制成的部件的改进的液相色谱系统可用于通过离子配对反相色谱法分离核酸片段，特别是双链核酸的大片段。钛，涂层不锈钢或聚合物组分不会将多价阳离子释放到流过色谱系统的水溶液中。或者除了利用由上述组分制成的材料之外，还可以使用放置在分离塔上游的多价阳离子捕获树脂从体系中除去多价离子。多价阳离子捕获树脂可以包含在保护盘，保护柱或防护盒中。通过离子对反相色谱法和回旋分离色谱法分离基于其分子量的核酸片段混合物的新方法利用上述液相色谱系统。

10. 发明授权

US5972222A Process for performing polynucleotide separations 失效
公开(公告)号：US5972222A
公开(公告)日：1999-10-26
申请号：US81039
申请日：1998-05-18
申请人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
发明人： Douglas T. Gjerde , Robert M. Haefele , David W. Togami
IPC分类号： B01D15/08 , B01D15/12 , B01D15/36 , B01D15/38 , C12N15/10 , G01N30/14 , G01N30/52
CPC分类号： B01J45/00 , B01D15/12 , B01D15/366 , B01D15/38 , C12N15/101 , G01N2030/528 , G01N30/14
摘要： The invention recognizes the deleterious effects of trace, and even undetectable amounts of multivalent cations on the separation of mixtures of polynucleotides, especially double stranded polynucleotides, and provides an improved method for separating such mixtures on wide pore, non-polar separation media by eliminating multivalent cations from the all aspects of the separation process. This is accomplished by using components in the separation process which are materials which do not release metal cations. In addition, the use of cation capture resins and other methods to remove residual traces of multivalent cations from eluting solvents, sample solutions, separation media, and system components is described. It is also important to remove any traces or organic contaminants from solvents solutions and system parts. Taking similar steps to remove residual traces of multivalent cations and organic impurities from the separation process, the invention may also be used in a batch process to separate mixtures of polynucleotide fragments.

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