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1. 发明授权

US08512978B2 Methods and compositions for a microemulsion-based tissue treatment 有权
标题翻译：用于微乳液组织处理的方法和组合物
公开(公告)号：US08512978B2
公开(公告)日：2013-08-20
申请号：US13644655
申请日：2012-10-04
申请人： Brian H. Kram , Christopher Bieniarz , Paul D. Drumheller
发明人： Brian H. Kram , Christopher Bieniarz , Paul D. Drumheller
IPC分类号： G01N1/30 , C12Q1/00 , B01F3/00
CPC分类号： G01N1/30
摘要： The invention is directed to methods and compositions for deparaffinizing paraffin-embedded biological samples for subsequent tissue staining. The compositions are microemulsions that may include water/oil/surfactant microemulsions, and optionally a cosurfactant. The microemulsions enable deparaffinization without the use of xylene or toluene, and also enable solvent exchange without the use of intermediary alcohol dehydration or alcohol rehydration compositions.
摘要翻译：本发明涉及用于随后组织染色的石蜡包埋生物样品脱蜡的方法和组合物。组合物是微乳液，其可以包括水/油/表面活性剂微乳液，以及任选的辅助表面活性剂。微乳液能够在不使用二甲苯或甲苯的情况下脱蜡，并且还可以在不使用中间醇脱水或醇补液组合物的情况下进行溶剂交换。

2. 发明授权

US08303915B2 Thin film apparatus and method 有权
标题翻译：薄膜装置及方法
公开(公告)号：US08303915B2
公开(公告)日：2012-11-06
申请号：US11926590
申请日：2007-10-29
申请人： Brian H. Kram , Vincent R. Rizzo , Ryan Reeser , David Chafin
发明人： Brian H. Kram , Vincent R. Rizzo , Ryan Reeser , David Chafin
IPC分类号： G01N1/31 , G01N35/02 , B05C5/02
CPC分类号： G01N1/2813 , G01N1/312 , G01N35/1002 , G01N35/1065 , G01N35/1081 , G01N2035/00039 , G01N2035/00138
摘要： A platen for contacting a liquid to a surface of a substantially flat substrate is disclosed. The platen includes a liquid application station and a stripping element at an end of the liquid application station, wherein the stripping element includes an intersecting gap and an air barrier. Also disclosed are an apparatus including the platen and a method of using the platen to contact a substrate with a liquid.
摘要翻译：公开了一种用于将液体接触到基本上平坦的基板的表面的压板。压板包括液体施加站和在液体施加站的端部处的剥离元件，其中剥离元件包括相交间隙和空气屏障。还公开了一种包括压板的装置和使用压板以与液体接触衬底的方法。

3. 发明授权

US07615371B2 Method and apparatus for treating a biological sample with a liquid reagent 有权
标题翻译：用液体试剂处理生物样品的方法和装置
公开(公告)号：US07615371B2
公开(公告)日：2009-11-10
申请号：US11016407
申请日：2004-12-17
申请人： Brian H. Kram
发明人： Brian H. Kram
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： G01N1/312 , B01L3/508 , B01L2200/0642 , B01L2200/0684 , B01L2300/0822 , G01N1/2813 , G01N2035/00158
摘要： A method and apparatus for treating a biological sample with a liquid reagent, comprising first and second substrates having facing surfaces defining a space therebetween in which the biological sample may be treated with the liquid reagent, wherein the first substrate comprises a relatively rigid fluid impermeable element while the second substrate comprises a relatively flexible liquid impermeable element.
摘要翻译：一种用液体试剂处理生物样品的方法和装置，包括第一和第二基底，其具有在其间限定空间的面对表面，其中可用液体试剂处理生物样品，其中第一基底包括相对刚性的流体不可渗透元件而第二衬底包括相对柔性的液体不可渗透元件。

4. 发明授权

US06306491B1 Respiratory aids 有权
标题翻译：呼吸辅助
公开(公告)号：US06306491B1
公开(公告)日：2001-10-23
申请号：US09282384
申请日：1999-03-31
申请人： Brian H. Kram , Stanley L. Mish , Michael J. Muehlbauer , James R. Bain
发明人： Brian H. Kram , Stanley L. Mish , Michael J. Muehlbauer , James R. Bain
IPC分类号： B32B302
CPC分类号： C12M45/22 , B01D69/00 , C12M23/08 , C12M23/24 , C12M29/04 , Y10T428/249954 , Y10T428/249955 , Y10T428/249976 , Y10T428/249978 , Y10T428/249981 , Y10T428/24999 , Y10T428/249994 , Y10T428/249999
摘要： The present invention is directed to materials that assist respiration of living cells contained in cell-containing systems. The materials form air-filled conduits or channels through which gases, such as oxygen and carbon dioxide, can readily exchange by diffusional means between regions of different gas partial pressures. When the present invention is placed within an aqueous environment, such as cell-culture media or host tissue, the invention provides aid to cellular respiration in cell-containing systems.
摘要翻译：本发明涉及辅助包含在含细胞系统中的活细胞呼吸的材料。这些材料形成空气填充的管道或通道，通过这些气体，诸如氧气和二氧化碳的气体可以通过扩散装置在不同气体分压区域之间容易地交换。当将本发明置于含水环境（例如细胞培养基或宿主组织）中时，本发明提供了对含细胞系统中的细胞呼吸的辅助。

5. 发明申请

US20130029376A1 METHODS AND COMPOSITIONS FOR A MICROEMULSION-BASED TISSUE TREATMENT 有权
标题翻译：用于基于微乳液的组织治疗的方法和组合物
公开(公告)号：US20130029376A1
公开(公告)日：2013-01-31
申请号：US13644655
申请日：2012-10-04
申请人： Brian H. Kram , Christopher Bieniarz , Paul D. Drumheller
发明人： Brian H. Kram , Christopher Bieniarz , Paul D. Drumheller
IPC分类号： G01N1/28
CPC分类号： G01N1/30
摘要： The invention is directed to methods and compositions for deparaffinizing paraffin-embedded biological samples for subsequent tissue staining The compositions are microemulsions that may include water/oil/surfactant microemulsions, and optionally a cosurfactant. The microemulsions enable deparaffinization without the use of xylene or toluene, and also enable solvent exchange without the use of intermediary alcohol dehydration or alcohol rehydration compositions.
摘要翻译：本发明涉及用于随后组织染色的石蜡包埋生物样品脱蜡的方法和组合物。组合物是微乳液，其可包括水/油/表面活性剂微乳液，以及任选的辅助表面活性剂。微乳液能够在不使用二甲苯或甲苯的情况下脱蜡，并且还可以在不使用中间醇脱水或醇补液组合物的情况下进行溶剂交换。

6. 发明申请

US20090104654A1 LOW VOLATILITY HIGH TEMPERATURE TISSUE CONDITIONING CROSS-REFERENCE TO RELATED APPLICATION 审中-公开
标题翻译：低挥发性高温组织调理相关申请的交叉参考
公开(公告)号：US20090104654A1
公开(公告)日：2009-04-23
申请号：US11720705
申请日：2005-12-14
申请人： Brian H. Kram , Christopher Bieniarz
发明人： Brian H. Kram , Christopher Bieniarz
IPC分类号： G01N1/30 , G01N1/31
CPC分类号： G01N1/30 , G01N1/312
摘要： Solutions exhibiting little or no evaporative loss at elevated temperatures, i.e., in excess of 100° C., are employed in place of conventional aqueous-based antigen retrieval solutions.
摘要翻译：使用在高温下（即超过100℃）几乎没有或没有蒸发损失的溶液代替常规的水性抗原检索溶液。

7. 发明授权

US08288121B2 Methods and compositions for a microemulsion-based tissue treatment 有权
标题翻译：用于微乳液组织处理的方法和组合物
公开(公告)号：US08288121B2
公开(公告)日：2012-10-16
申请号：US12165020
申请日：2008-06-30
申请人： Brian H. Kram , Christopher Bieniarz , Paul D. Drumheller
发明人： Brian H. Kram , Christopher Bieniarz , Paul D. Drumheller
IPC分类号： G01N1/30 , C12Q1/00 , B01F3/00
CPC分类号： G01N1/30
摘要： The invention is directed to methods and compositions for deparaffinizing paraffin-embedded biological samples for subsequent tissue staining. The compositions are microemulsions that may include water/oil/surfactant microemulsions, and optionally a cosurfactant. The microemulsions enable deparaffinization without the use of xylene or toluene, and also enable solvent exchange without the use of intermediary alcohol dehydration or alcohol rehydration compositions.
摘要翻译：本发明涉及用于随后组织染色的石蜡包埋生物样品脱蜡的方法和组合物。组合物是微乳液，其可以包括水/油/表面活性剂微乳液，以及任选的辅助表面活性剂。微乳液能够在不使用二甲苯或甲苯的情况下脱蜡，并且还可以在不使用中间醇脱水或醇补液组合物的情况下进行溶剂交换。

8. 发明申请

US20090253592A1 METHOD AND APPARATUS FOR TREATING A BIOLOGICAL SAMPLE WITH A LIQUID REAGENT 审中-公开
标题翻译：用液体试剂处理生物样品的方法和装置
公开(公告)号：US20090253592A1
公开(公告)日：2009-10-08
申请号：US12484114
申请日：2009-06-12
申请人： Brian H. Kram
发明人： Brian H. Kram
IPC分类号： C40B60/08 , C12M1/34
CPC分类号： G01N1/312 , B01L3/508 , B01L2200/0642 , B01L2200/0684 , B01L2300/0822 , G01N1/2813 , G01N2035/00158
摘要： A method and apparatus for thin film fluid processing of biological samples without rinsing between treatments is provided. An apparatus having a treatment zone for treating a biological sample with a liquid reagent, comprising first and second substrates having facing surfaces defining a space therebetween in which the biological sample may be treated with the liquid reagent, wherein the first substrate comprises a relatively fluid impermeable element while the second substrate comprises a relatively flexible gas permeable element.
摘要翻译：提供了一种在处理之间没有漂洗的生物样品的薄膜流体处理方法和装置。一种具有用于用液体试剂处理生物样品的处理区的设备，包括具有限定其间的空间的面对表面的第一和第二基底，其中可用液体试剂处理生物样品，其中第一基底包括相对流体不渗透性而第二衬底包括相对柔性的气体可渗透元件。

9. 发明授权

US06617151B1 Method of closing a cell containment device with a wet seal 有权
标题翻译：用湿密封封闭电池容纳装置的方法
公开(公告)号：US06617151B1
公开(公告)日：2003-09-09
申请号：US09515264
申请日：2000-02-29
申请人： Steven C. Newman , Brian H. Kram , Terry A Hubbard
发明人： Steven C. Newman , Brian H. Kram , Terry A Hubbard
IPC分类号： C12M100
CPC分类号： A61K9/0024 , A61F2/022
摘要： A method is provided for closing a containment device that involves wetting at least a portion of a permeable polymeric membrane such as tubular membrane with a liquid such as a cell suspension or drug formulation the device is being filled with, and applying heat to at least a portion of a wetted thermoplastic polymer in association with the membrane to form a “wet seal” closure. The thermoplastic polymer melts at a lower temperature than the polymeric membrane and the melted polymer integrates with the membrane by flowing along surfaces and into available interstices of the membrane to form a cell-tight closure when the polymer cools below its melt temperature. The application of heat may be accompanied by slight pressure, and a heat sink may be applied to limit heat transfer beyond the closure region to the membrane. In association with melting of the polymer, the membrane/thermoplastic polymer combination may be twisted and/or elongated, and twisting may form a separation region which can be cut to form first and second devices. The membrane and thermoplastic polymer may be made of polytetrafluoroethylene and fluorinated ethylene propylene, respectively. The containment device may be directly implanted into a recipient in need of a treatment provided by contents of the device.
摘要翻译：提供了一种封闭容纳装置的方法，该容纳装置涉及用例如细胞悬浮液或装置所填充的药物制剂的液体润湿至少一部分可渗透的聚合物膜（例如管状膜），并将热量施加至至少一种与膜结合的湿润热塑性聚合物的一部分以形成“湿密封”封闭。热塑性聚合物在比聚合物膜低的温度下熔化，并且当聚合物冷却到低于其熔融温度时，熔融的聚合物通过沿着表面流动并进入膜的可用间隙而形成细胞密封的闭合物。热的施加可能伴随着轻微的压力，并且可以施加散热器以限制超过封闭区域的热传递到膜。与聚合物的熔融相关联，膜/热塑性聚合物组合可以被扭曲和/或伸长，并且扭转可以形成可以被切割以形成第一和第二装置的分离区域。膜和热塑性聚合物可以分别由聚四氟乙烯和氟化乙烯丙烯制成。容纳装置可以直接植入到需要由装置的内容物提供的处理的接收器中。

10. 发明申请

US20090170152A1 Tissue Conditioning Protocols 审中-公开
标题翻译：组织调理方案
公开(公告)号：US20090170152A1
公开(公告)日：2009-07-02
申请号：US12344564
申请日：2008-12-28
申请人： Ryan Reeser , Brian H. Kram , Vincent R. Rizzo , David Chafin , Jerome W. Kosmeder , Christopher Bieniarz
发明人： Ryan Reeser , Brian H. Kram , Vincent R. Rizzo , David Chafin , Jerome W. Kosmeder , Christopher Bieniarz
IPC分类号： G01N1/28
CPC分类号： G01N1/30 , G01N1/312 , G01N1/36
摘要： Solutions exhibiting little or no evaporative loss at elevated temperatures, i.e., in excess of 100° C., are employed in place of conventional aqueous-based antigen retrieval solutions.
摘要翻译：使用在高温下（即超过100℃）几乎没有或没有蒸发损失的溶液代替常规的水性抗原检索溶液。

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