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1. 发明授权

US4477578A Method and apparatus for performing assays 失效
标题翻译：用于进行测定的方法和装置
公开(公告)号：US4477578A
公开(公告)日：1984-10-16
申请号：US354848
申请日：1982-03-04
申请人： Laughton E. Miles , Arthur H. Rogers, Jr. , Charles H. Rogers
发明人： Laughton E. Miles , Arthur H. Rogers, Jr. , Charles H. Rogers
IPC分类号： G01N1/38 , G01N33/483 , G01N33/53 , G01N33/543 , G01N33/58 , G01N35/00 , G01N35/10 , G01N33/54 , G01N33/56 , G01N35/04 , G01N35/06
CPC分类号： G01N35/1065 , G01N1/38 , G01N33/5302 , G01N33/54366 , G01N35/00 , G01N2035/00544 , G01N2035/1055 , G01N35/1079 , Y10S436/807 , Y10S436/808 , Y10S436/81 , Y10S436/82 , Y10T436/113332
摘要： Method and apparatus are provided for carrying out multiple simultaneous transfers of fluid. The method and apparatus are particularly directed toward immunoassays wherein immunologically active compounds, such as antigens and haptens, are detected through their associated antibodies. The device relies on the ability to transfer fluids, such as biological samples and reagents, between a reservoir and an associated receptacle. By providing a receptacle having a port at its lower end and which is otherwise hermetically sealed, such fluid transfer can be effected by immersing the port beneath the surface of the fluid in the reservoir and manipulating the pressure on the remaining surface area outside the port. The transfer of biological fluids at positive pressure provides enhanced fluids flow characteristics, particularly reduction or elimination of the tendency of these fluids to froth or bubble. Moreover, since the fluids can easily be manipulated, they can be agitated to speed up the reaction and reduce the overall reaction time and can be transferred from the reaction zone to allow interim measurements of the extent of reaction to provide for a rate mode assay. The method and apparatus also find use in preparing solid phase reagents for use in assay systems, as well as a highly accurate pipetting system in analytic applications not limited to immunoassays.
摘要翻译：提供了用于执行多次同时传送流体的方法和装置。该方法和装置特别针对免疫测定，其中通过其相关抗体检测免疫活性化合物，例如抗原和半抗原。该装置依赖于在储存器和相关容器之间传送流体（例如生物样品和试剂）的能力。通过提供在其下端具有端口并且以其它方式密封的插座，可以通过将端口在流体表面下方浸入储存器中并且操纵在端口外部的剩余表面区域上的压力来实现这种流体转移。生物流体在正压下的转移提供增强的流体流动特性，特别是减少或消除这些流体发泡或起泡的倾向。此外，由于可以容易地操纵流体，因此可以搅拌它们以加速反应并减少总反应时间，并且可以从反应区转移以允许临时测量反应程度以提供速率模式测定。该方法和装置还可用于制备用于测定系统的固相试剂，以及用于不限于免疫测定的分析应用中的高精度移液系统。

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