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    • 4. 发明授权
    • Electrochemical noise measurement to assess cellular behavior and
metabolic activity
    • 电化学噪声测量以评估细胞行为和代谢活动
    • US6010889A
    • 2000-01-04
    • US817290
    • 1997-06-23
    • David GeareyDavid Edward WoolleyRobert David Eden
    • David GeareyDavid Edward WoolleyRobert David Eden
    • C12M1/34C12M3/04G01N33/487C12N13/00C12Q1/02G01N27/26
    • G01N33/4833
    • A method and apparatus is presented for analyzing directly obtained electrochemical noise measurements at cellular surfaces to assess cellular behavior and metabolic activity. The apparatus comprises a container (1) into which culture medium containing the cells to be assessed (2) has been introduced. Located within the container and partially submerged by the culture medium is a main electrode (3) which is a chemically inert electrically conductive surface such as gold film. This electrically conductive surface supports the adherent, viable cells to be assessed, and which may have been previously grown on the electrode to near confluence. The container is closed by a lid (4) which is penetrated by a tube (5) filled with an electrochemically conducting medium that is in electrochemical contact with a reference electrode. The end of the tube (5) is immersed in the culture medium. A further tube is provided through which test factors such as stimulants or suppressants can be injected into the container as indicated by the arrow. The directly measured electrochemical noise reflects metabolic activity of the cell at the cell surface. Fluctuations in the electrochemical noise are analyzed in the conventional manner and correlated with cellular activity.
    • PCT No.PCT / GB95 / 02297第 371日期:1997年6月23日 102(e)日期1997年6月23日PCT提交1995年9月29日PCT公布。 公开号WO96 / 10742 日期1996年4月11日提出了一种用于分析直接获得的细胞表面的电化学噪声测量以评估细胞行为和代谢活性的方法和装置。 该装置包括容器(1),其中已经引入含有待评估细胞(2)的培养基。 位于容器内并被培养基部分浸没的是主要电极(3),其是诸如金膜的化学惰性导电表面。 该导电表面支持待评估的粘附的活细胞,并且其可以先前在电极上生长至接近汇合。 容器被盖(4)封闭,盖子(4)被填充有与参比电极电化学接触的电化学传导介质的管(5)穿透。 将管(5)的末端浸入培养基中。 提供另一个管,如箭头所示,可以将诸如兴奋剂或抑制剂的测试因子注入容器中。 直接测量的电化学噪声反映了细胞表面细胞的代谢活性。 电化学噪声的波动以常规方式分析,并与细胞活性相关。
    • 8. 发明授权
    • Coupling
    • 耦合
    • US5527073A
    • 1996-06-18
    • US185788
    • 1994-01-21
    • Matthew J. Readman
    • Matthew J. Readman
    • F16L37/091F16L19/08
    • F16L37/091Y10S285/902
    • A coupling comprising a first member defining a socket, a second number such as a tube defining a spigot inserted into an open end of the socket, and a locking device received within an annular gap defined between an inner wall of the socket and an outer wall of the spigot. The locking device comprises circumferentially spaced load-bearing members, each load-bearing member extending within the annular gap from a first end which is relatively close to the open end of the socket and a second end which is relatively remote from the open end of the socket. Each load-bearing member is resiliently biased such that the first end engages the socket and the second end engages the spigot, and each load-bearing member is dimensioned such that it acts substantially as a beam in compression to resist withdrawal of the spigot from the socket.
    • PCT No.PCT / GB92 / 01363 Sec。 371日期1994年1月21日 102(e)日期1994年1月21日PCT提交1992年7月23日PCT公布。 出版物WO93 / 02315 日期:1993年2月4日。一种联接器,包括限定插座的第一构件,第二数量,例如限定插入到插座的开口端中的插头的管,以及容纳在限定在内壁 的插座和插口的外壁。 锁定装置包括周向隔开的承载构件,每个承载构件从相对接近插座的开口端的第一端在环形间隙内延伸,并且第二端相对远离插座的开口端 插座。 每个承载构件被弹性地偏置,使得第一端接合插座,并且第二端接合插口,并且每个承载构件的尺寸被设计成使得其基本上作为压缩的梁起作用以阻止插口从 插座。
    • 9. 发明授权
    • Polymer compositions
    • 聚合物组合物
    • US5516819A
    • 1996-05-14
    • US295610
    • 1994-08-25
    • Stephen F. BushJames M. MethvenDavid R. Blackburn
    • Stephen F. BushJames M. MethvenDavid R. Blackburn
    • C08G18/48C08J3/00C08L67/06C08L75/16
    • C08L67/06C08G18/4879C08J3/005C08L75/16
    • A method of producing a thickened organic polymeric composition useful for molding and capable of resisting post-molding shrinkage after being crosslinked comprising a cross-linkable base resin dissolved in an unsaturated monomer, and an additive resin selected from saturated polyesters and saturated amide waxes, the additive resin being crystalline at ambient temperatures and having a melting point (T.sub.m) below a temperature (T.sub.c) at which the base resin cross-linking reaction proceeds at a significant rate. The base resin and additive resin have only a partial degree of compatibility. When cooled from a temperature between T.sub.m and T.sub.c to temperature between T.sub.m and ambient the composition thickens, whereas, when it is heated to a temperature below T.sub.c, it reverts to a flowable composition.
    • 一种生产可用于模塑并且能够抵抗交联后的后成型收缩的增稠的有机聚合物组合物的方法,包括溶解在不饱和单体中的可交联的基础树脂和选自饱和聚酯和饱和酰胺蜡的添加剂树脂, 添加剂树脂在环境温度下为结晶,并且具有低于基本树脂交联反应以显着速率进行的温度(Tc)的熔点(Tm)。 基础树脂和添加剂树脂仅具有部分相容性。 当从Tm和Tc之间的温度冷却到Tm和环境温度之间时,组合物变厚,而当它被加热到低于Tc的温度时,它回复到可流动的组合物。
    • 10. 发明授权
    • Growth of biomass
    • 生物量的增长
    • US4545909A
    • 1985-10-08
    • US557318
    • 1983-12-02
    • Bernard AtkinsonGeoffrey M. BlackAnthony PinchesPaul J. S. Lewis
    • Bernard AtkinsonGeoffrey M. BlackAnthony PinchesPaul J. S. Lewis
    • C02F3/08C02F3/06C02F3/10C02F3/12C12M1/00C12M1/04C12M1/16C12M1/40C12N1/00
    • C02F3/085C02F3/06C02F3/10C12M25/14C12M29/06Y02W10/15
    • This invention provides a process and apparatus for the growth of biomass from a supply of suitable nutrient wherein the volume of biomass and the size and shape of the units in which it is present within a vessel (10) in which the reaction takes place can be accurately controlled. Control is achieved by providing a support structure (14) for the biomass comprising one or more movable bodies each of which has an internal reticular structure defining a substantial voidage therein and which essentially fills with the biomass but which does not overfill since excess growth is restricted. Restriction can be achieved by removal of excess biomass or by removal of the bodies at the time that they become filled. The techniques are capable of numerous industrial applications including various biological fermentation processes as well as the purification of sewage and industrial effluents, for example.
    • 本发明提供了一种用于从合适营养物供应生长生物质的方法和装置,其中生物量的体积以及存在于发生反应的容器(10)内的单元的尺寸和形状可以是 准确控制。 通过提供用于生物质的支撑结构(14)来实现控制,所述支撑结构(14)包括一个或多个可移动体,每个移动体具有在其中限定实质空隙的内部网状结构,并且其基本上填充有生物质,但是由于过度生长被限制 。 可以通过去除多余的生物质或通过在它们被填充时去除体来实现限制。 这些技术能够具有许多工业应用,例如各种生物发酵方法以及污水和工业废水的净化。