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1. 发明公开

US20240336705A1 METHOD FOR PREPARING RESISTANT DEXTRIN 审中-公开
公开(公告)号：US20240336705A1
公开(公告)日：2024-10-10
申请号：US18575020
申请日：2022-11-07
申请人： SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD.
发明人： Zhaobo GAN , Qian DU , Mingzhan ZHANG , Shuangshuang LIU
IPC分类号： C08B30/18
CPC分类号： C08B30/18
摘要： The present application relates to a method for preparing resistant dextrin, and belongs to the technical field deep processing of starch. By means of the present disclosure, proteins are firstly removed from starch milk, and then hydrolysis, evaporation concentration, polymerization, refining and purification are sequentially carried out to finally obtain a resistant dextrin, and by means of controlling the content of proteins in the starch milk, Maillard reaction is effectively reduced, the content of dietary fibers in a crude product and the light transmittance of a product are increased, the sharing of subsequent refining and purification is reduced, the consumption of activated carbon and acid and alkali is reduced, and the cost of refining and purification is reduced.

2. 发明授权

US10479840B2 Resistant dextrin and method for preparing the same 有权
公开(公告)号：US10479840B2
公开(公告)日：2019-11-19
申请号：US15566234
申请日：2017-01-17
申请人： SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD
发明人： Guangpeng Dou , Zhaobo Gan , Fanghua Li , Xianbao Shao , Tengteng Yang , Qian Du , Mingzhan Zhang , Xingjing Zhang
IPC分类号： C08B30/18 , A23L29/30 , A23L33/26 , B01D15/18 , B01D15/36
摘要： A resistant dextrin and a method for preparing the same. In the resistant dextrin, molar content of DP1 is smaller than or equal to 1%, molar content of DP2 is smaller than or equal to 4.5%, molar content of DP3 is smaller than or equal to 7.0%, an average degree of polymerization is 6-12, a pH value is 3.0-6.0, and molar content of dietary fibers is greater than or equal to 85%. The present invention further relates to a method for preparing the resistant dextrin. Since the present invention uses starch as the raw material and divides dextrinization reaction into two stages for the first time, the degree of polymerization of the polymerization reaction is reliably guaranteed, the number of caused side reactions is decreased at the same time and the difficulty in subsequent purification treatment is greatly decreased.

3. 发明授权

US11149048B2 High-purity D-psicose preparation method 有权
公开(公告)号：US11149048B2
公开(公告)日：2021-10-19
申请号：US16466060
申请日：2017-12-01
申请人： SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD
发明人： Baode Dou , Guangpeng Dou , Mingzhan Zhang , Xianbao Shao , Fanghua Li , Qian Du , Tengteng Yang
IPC分类号： C07H1/08 , C07H3/02 , C12P19/02 , C12P19/24
摘要： A method for preparing high-purity D-psicose, comprising the following steps: (1) centrifuging a fermentation broth of Bacillus subtilis, and then subjecting the bacteria to homogenization to obtain a mixed solution containing D-psicose 3-epimerase; (2) preparing a fructose solution, adding the mixed solution containing D-psicose 3-epimerase to the fructose solution, adjusting the pH, adding cobalt chloride thereto, and performing the reaction at a certain temperature; and feeding the fructose solution to the reaction solution, continuing the reaction, and stopping the reaction, obtaining a crude D-psicose solution; and (3) subjecting the crude D-psicose solution to decolorization, filtration, ion exchange, chromatographic separation, concentration, and then crystallization or drying, obtaining D-psicose.

4. 发明申请

US20210139938A1 ASPERGILLUS ORYZAE BLCY-006 STRAIN AND APPLICATION THEREOF IN PREPARATION OF GALACTOOLIGOSACCHARIDE 有权
公开(公告)号：US20210139938A1
公开(公告)日：2021-05-13
申请号：US17251125
申请日：2020-02-18
申请人： SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD.
发明人： Zhaobo GAN , Fanghua LI , Guangpeng DOU , Xianbao SHAO , Mingzhan ZHANG , Qian DU , Tengteng YANG
IPC分类号： C12P19/14 , C12R1/69 , C12N1/14 , C12N9/38
摘要： Provided are an aspergillus oryzae BLCY-006 strain and an application thereof in the preparation of a galactooligosaccharide. The strain produces β-galactosidase, and the enzyme activity can reach 300 U/ml after culturing and fermentation, which is more than 50% higher than traditional β-galactosidase activity. The enzyme also has lactose and glucose resistance properties.

5. 发明授权

US11780870B2 Method for preparing crystalline D-psicose 有权
公开(公告)号：US11780870B2
公开(公告)日：2023-10-10
申请号：US18011614
申请日：2021-12-03
申请人： SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD.
发明人： Zhaobo Gan , Xianbao Shao , Fanghua Li , Qian Du , Xingjing Zhang , Shuangshuang Liu
IPC分类号： C07H1/06 , C07H3/02
CPC分类号： C07H1/06 , C07H3/02 , C07B2200/13
摘要： The present application relates to a method for preparing crystalline D-psicose. In the present application, crystalline D-psicose is prepared by means of combining an evaporative crystallization method and a cooling crystallization method, so that the preparation process is simple, the operation is easy, and the crystallization period is short. In the present application, a seed crystal is added when the solid content is relatively low (70-75%) in an evaporative crystallization process, so that the crystallization process is facilitated, the growth speed of the crystal in each direction is more uniform, the whole crystallization period can be shortened, and the energy consumption is reduced; and after the evaporative crystallization is finished, crystal regulation is carried out, so that the crystal size tends to be equalized, the crystal surface grows to be smooth, and the crystal form is perfected. Cooling crystallization is carried out after the crystal regulation, and the cooling frequency of specific cooling intervals is designed in the cooling process according to the metastable region curve of D-psicose. After crystallization by means of the preparation method of the present application is finished, the obtained crystal has a high yield, a narrow crystal size distribution, a smooth crystal surface, a mirror-like surface, good reflectivity and a regular crystal form.

6. 发明公开

US20230287026A1 METHOD FOR PREPARING CRYSTALLINE D-PSICOSE 审中-公开
公开(公告)号：US20230287026A1
公开(公告)日：2023-09-14
申请号：US18011614
申请日：2021-12-03
申请人： SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD
发明人： Zhaobo GAN , Xianbao SHAO , Fanghua LI , Qian DU, , Xingjing ZHANG , Shuangshuang LIU
IPC分类号： C07H1/06 , C07H3/02
CPC分类号： C07H1/06 , C07H3/02 , C07B2200/13
摘要： The present application relates to a method for preparing crystalline D-psicose. In the present application, crystalline D-psicose is prepared by means of combining an evaporative crystallization method and a cooling crystallization method, so that the preparation process is simple, the operation is easy, and the crystallization period is short. In the present application, a seed crystal is added when the solid content is relatively low (70-75%) in an evaporative crystallization process, so that the crystallization process is facilitated, the growth speed of the crystal in each direction is more uniform, the whole crystallization period can be shortened, and the energy consumption is reduced; and after the evaporative crystallization is finished, crystal regulation is carried out, so that the crystal size tends to be equalized, the crystal surface grows to be smooth, and the crystal form is perfected. Cooling crystallization is carried out after the crystal regulation, and the cooling frequency of specific cooling intervals is designed in the cooling process according to the metastable region curve of D-psicose. After crystallization by means of the preparation method of the present application is finished, the obtained crystal has a high yield, a narrow crystal size distribution, a smooth crystal surface, a mirror-like surface, good reflectivity and a regular crystal form.

7. 发明申请

US20220186272A1 STRAIN OF TRICHODERMA REESEI AND CULTURE METHOD AND USE THEREOF 有权
公开(公告)号：US20220186272A1
公开(公告)日：2022-06-16
申请号：US17253082
申请日：2020-07-06
申请人： SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD
发明人： Baode DOU , Guangpeng DOU , Zhaobo GAN , Xianbao SHAO , Fanghua LI , Qian DU
IPC分类号： C12P19/14 , C12N1/14 , C12N9/24 , C12P21/00
摘要： The invention relates to a strain of Trichoderma reesei BLCY-007 and its application in the production of xylooligosaccharides.

8. 发明授权

US11279961B2 Aspergillus oryzae BLCY-006 strain and application thereof in preparation of galactooligosaccharide 有权
公开(公告)号：US11279961B2
公开(公告)日：2022-03-22
申请号：US17251125
申请日：2020-02-18
申请人： SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD
发明人： Fanghua Li , Zhaobo Gan , Guangpeng Dou , Xianbao Shao , Mingzhan Zhang , Qian Du , Tengteng Yang
IPC分类号： C12P19/14 , C12N1/14 , C12N9/38 , C12R1/69
摘要： Provided are an Aspergillus oryzae BLCY-006 strain and an application thereof in the preparation of a galactooligosaccharide. The strain produces β-galactosidase, and the enzyme activity can reach 300 U/ml after culturing and fermentation, which is more than 50% higher than traditional β-galactosidase activity. The enzyme also has lactose and glucose resistance properties.

9. 发明授权

US10968424B2 Bacillus subtilis strain, culture method and use thereof 有权
公开(公告)号：US10968424B2
公开(公告)日：2021-04-06
申请号：US16318358
申请日：2017-11-28
申请人： SHANDONG BAILONG CHUANGYUAN BIO-TECH CO., LTD
发明人： Guangpeng Dou , Xianbao Shao , Qian Du , Zhaobo Gan , Fanghua Li , Mingzhan Zhang , Tengteng Yang , Shuangshuang Liu
IPC分类号： C12N1/20 , C12N9/90 , C12R1/125 , C12P19/24 , C12P19/02
摘要： A Bacillus subtilis strain or a progeny thereof, a method of culturing the same and uses thereof. The invention also relates to a culture and lysate of the Bacillus subtilis strain or its progeny, and a method for producing D-psicose epimerase and producing D-psicose using the Bacillus subtilis strain or its progeny.

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