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1. 发明授权

US4002532A Enzyme conjugates 失效
标题翻译：酶缀合物
公开(公告)号：US4002532A
公开(公告)日：1977-01-11
申请号：US516331
申请日：1974-10-21
申请人： Joel K. Weltman , M. Boris Rotman
发明人： Joel K. Weltman , M. Boris Rotman
IPC分类号： G01N33/535 , G01N33/68 , G01N31/14 , C07G7/02
CPC分类号： G01N33/535 , G01N33/6854 , Y10S435/962 , Y10S435/964 , Y10S435/969 , Y10S436/817 , Y10S530/814 , Y10S530/862
摘要： This invention relates to the provision of enzyme-macromolecule conjugates by conjugation with polyfunctional reagents in the presence of organic compounds which act as conditioners. Conjugation of enzymes with antibodies in the presence of conditioner yields conjugates with increased immunological specificity. Organic polyamines of either high or low molecular weight are conditioners. Conditioners are especially useful for conjugation of polymeric and unstable enzymes such as .beta.-D-galactosidase. The high degree of immunospecificity and sensitivity achieved with conditioners yields conjugates suitable for detection and quantification of substances, such as IgE, in nanogram quantities in biological fluids with many interfering substances.
摘要翻译：本发明涉及通过在作为调理剂的有机化合物存在下与多官能试剂缀合来提供酶 - 大分子缀合物。在调理剂存在下，酶与抗体的缀合产生具有增加的免疫学特异性的缀合物。高分子量或低分子量的有机多胺是调理剂。调理剂对于聚合和不稳定酶如β-D-半乳糖苷酶的缀合特别有用。通过调节剂实现的高度的免疫特异性和灵敏度产生适合于在具有许多干扰物质的生物液体中以纳刻量检测和定量物质（例如IgE）的缀合物。

2. 发明授权

US5792617A Cell proliferation-based amplified detection of analytes 失效
标题翻译：基于细胞增殖的分析物扩增检测
公开(公告)号：US5792617A
公开(公告)日：1998-08-11
申请号：US476229
申请日：1995-06-07
申请人： M. Boris Rotman
发明人： M. Boris Rotman
IPC分类号： G01N33/532 , C12Q1/02 , C12Q1/18 , C12Q1/68 , G01N33/53 , G01N33/569 , G01N33/574 , G01N33/58
CPC分类号： C12Q1/18 , G01N33/5308 , G01N33/56966 , G01N33/56972 , G01N33/57484 , G01N33/57492 , G01N33/58 , G01N33/581 , Y10S435/834 , Y10S435/839 , Y10S435/848 , Y10S435/878 , Y10S435/881 , Y10S435/883 , Y10S435/966 , Y10S436/807 , Y10S436/808 , Y10S436/813
摘要： A test kit and method for the highly sensitive detection of specific analytes in a sample is provided. The presence of the analyte in the sample results in a decrease in the concentration of a growth inhibiting substance leading to proliferation of cells in the region of the analyte. The presence or absence of the analyte is determined by detecting the presence of increased numbers of cells. Assay sensitivity is accounted for by the exponential amplification of cell number that occurs during cell proliferation in the presence of analyte.
摘要翻译：提供了一种用于样品中特异性分析物的高灵敏度检测的测试试剂盒和方法。样品中分析物的存在导致生长抑制物质的浓度降低，导致分析物区域中的细胞增殖。分析物的存在或不存在通过检测增加数目的细胞的存在来确定。在分析物存在下细胞增殖期间细胞数量的指数扩增可以解释测定灵敏度。

3. 发明授权

US5472846A Test kit and method for amplification and detection of antigen cells 失效
标题翻译：用于扩增和检测抗原细胞的试剂盒和方法
公开(公告)号：US5472846A
公开(公告)日：1995-12-05
申请号：US292416
申请日：1994-08-18
申请人： M. Boris Rotman
发明人： M. Boris Rotman
IPC分类号： C12Q1/18 , G01N33/53 , G01N33/569 , G01N33/574 , G01N33/58
CPC分类号： C12Q1/18 , G01N33/5308 , G01N33/56966 , G01N33/56972 , G01N33/57484 , G01N33/57492 , G01N33/58 , G01N33/581 , Y10S435/834 , Y10S435/839 , Y10S435/848 , Y10S435/874 , Y10S435/881 , Y10S435/883 , Y10S436/813
摘要： A test kit and method for the amplification and detection of specific antigen cells using a probe. The method includes reacting the probe-specific cells with enzyme-conjugated molecules to form separate molecules. The specific antigen cells are mixed with a selected antibiotic which antibiotic is adversely affected by the enzyme in the reporter molecules and incubating the mixture to promote a bacterial chain reaction forming satellite colonies of bacteria microcolonies about the specific cells which amplifies the cells. The method then includes detecting the amplified probe-specific cells by observing the satellite colonies.
摘要翻译：使用探针扩增和检测特异性抗原细胞的测试试剂盒和方法。该方法包括使探针特异性细胞与酶共轭分子反应以形成分开的分子。将特异性抗原细胞与选定的抗生素混合，抗生素受报告分子中的酶的不利影响，并孵育该混合物以促进细菌链反应，形成关于放大细胞的特定细胞的细菌微菌落的卫星菌落。该方法然后包括通过观察卫星菌落来检测扩增的探针特异性细胞。

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