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1. 发明授权

US5561052A Process for detecting oxidized lipids and process for forming oxidized lipids 失效
标题翻译：检测氧化脂质的方法和形成氧化脂质的方法
公开(公告)号：US5561052A
公开(公告)日：1996-10-01
申请号：US446082
申请日：1995-05-19
申请人： Katsumasa Koike
发明人： Katsumasa Koike
IPC分类号： G01N33/92 , C12Q1/26 , G01N33/00
CPC分类号： G01N33/92 , Y10S435/968 , Y10T436/104165 , Y10T436/24 , Y10T436/25
摘要： There are disclosed a process for detecting and determining an oxidized lipid in a specimen, which is capable of readily and accurately determining a specimen as containing an oxidized lipid, and a process for forming a water-soluble oxidized lipid having a hydroperoxide group which has specific influence on a living body. A specimen is detected and determined to contain an oxidized lipid by adding a lanthanide shift reagent to a specimen, followed by spectroscopic analysis thereof. An oxidized lipid is formed by adding superoxide dismutase (SOD) and CuSO.sub.4 to (1) an emulsion prepared by dissolving linoleic acid or arachidonic acid in deuterated methyl alcohol and adding the solution to a deuterated phosphate buffer under stirring, or to (2) a low density lipoprotein solution sufficiently dialyzed against an undeuterated phosphate buffer; followed by irradiation with long-wave ultraviolet light.
摘要翻译：公开了一种用于检测和测定样品中的氧化脂质的方法，其能够容易且准确地测定含有氧化脂质的样品，以及形成具有氢过氧化物基团的水溶性氧化脂质的方法，所述氢过氧化物基具有特定的对活体的影响。通过向样品中加入镧系元素转移试剂，然后进行光谱分析，检测并测定样品含有氧化脂质。通过向（1）通过将亚油酸或花生四烯酸溶解在氘代甲醇中制备的乳液中加入超氧化物歧化酶（SOD）和CuSO 4来形成氧化脂质，并将其加入到氘代磷酸盐缓冲液中，或（2）低密度脂蛋白溶液相对于未氘代磷酸盐缓冲液充分透析; 随后用长波紫外线照射。

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