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1. 发明授权

US4675286A Fetal cell separation and testing 失效
标题翻译：胎儿细胞分离和检测
公开(公告)号：US4675286A
公开(公告)日：1987-06-23
申请号：US695971
申请日：1985-01-28
申请人： Emanuel Calenoff
发明人： Emanuel Calenoff
IPC分类号： A61B10/00 , A61B10/02 , C12Q1/24 , G01N33/569 , G01N33/543 , G01N33/554 , G01N33/577
CPC分类号： A61B10/02 , C12Q1/24 , G01N33/56966 , Y10T436/25375 , Y10T436/255
摘要： A method for obtaining fetal cells for diagnostic examination comprises removing detached cells from the uterine cavity and outer surface of the amnionic sac. The cells are then incubated in the presence of a separation antibody which binds preferentially to either fetal cell antigens or maternal cell antigens for a time sufficient to permit antibody-antigen binding to occur. Cells having said separation antibody bound thereto are separated for the mixture. The separation antibody can be an anitbody binding preferentially to fetal cell antigens and not significantly to maternal cell antigens or it can be an antibody binding preferentially to maternal cell antigens and not significantly to fetal cell antigens. The antibody can be bound to an insoluble support prior to the incubation, and separation can be effected by separating the insoluble support from the cell mixture following the incubation. Alternatively, the separation antibody can be fluorescent labeled, and cells conjugated with fluorescent labeled antibody can be removed using a cell sorter. The isolated fetal cells are then propagated to obtain a population sufficient for diagnostic examination.
摘要翻译：用于获得用于诊断检查的胎儿细胞的方法包括从子宫腔和羊膜囊的外表面去除分离的细胞。然后将细胞在分离抗体存在下孵育，所述分离抗体优先结合胎儿细胞抗原或母体细胞抗原足以允许发生抗体 - 抗原结合的时间。分离具有与之结合的分离抗体的细胞用于混合物。分离抗体可以是优先结合胎儿细胞抗原的抗体，而不是显着地与母体细胞抗原结合，或者它可以是优先与母体细胞抗原结合的抗体，而不是显着地与胎儿细胞抗原结合。在孵育之前，抗体可以结合到不溶性载体上，并且可以通过在孵育后从细胞混合物中分离不溶性载体来进行分离。或者，分离抗体可以是荧光标记的，并且可以使用细胞分选机除去与荧光标记抗体缀合的细胞。然后将分离的胎儿细胞繁殖以获得足以进行诊断检查的群体。

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