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1. 发明申请

US20150232884A1 Genetically Enhanced Cyanobacteria for the Production of Isoprene 审中-公开
标题翻译：用于生产异戊二烯的转基因蓝藻
公开(公告)号：US20150232884A1
公开(公告)日：2015-08-20
申请号：US14640915
申请日：2015-03-06
申请人： ALGENOL BIOFUELS INC.
发明人： Ulf DUEHRING
IPC分类号： C12P5/00 , C12N1/20 , C12N9/88
CPC分类号： C12P5/007 , C12N1/20 , C12N9/88 , C12N15/74 , C12Y402/03027
摘要： A cyanobacterium for the production of isoprene having an extrachromosomal plasmid harboring a gene for the production of isoprene. Such a cyanobacterium exhibits a higher isoprene production rate than other conventional strains.
摘要翻译：用于生产异戊二烯的蓝细菌，其具有含有用于生产异戊二烯的基因的染色体外质粒。这样的蓝细菌表现出比其他常规菌株更高的异戊二烯生产速率。

2. 发明申请

US20140322799A1 METABOLICALLY ENHANCED CYANOBACTERIUM WITH SEQUENTIALLY INDUCIBLE PRODUCTION GENES FOR THE PRODUCTION OF A FIRST CHEMICAL COMPOUND 有权
标题翻译：具有生产第一化学化合物的具有连续可诱导生产基因的代谢增强的细胞因子
公开(公告)号：US20140322799A1
公开(公告)日：2014-10-30
申请号：US14320477
申请日：2014-06-30
申请人： Algenol Biofuels Inc.
发明人： Ulf Dühring , Alexandra FRIEDRICH , Kerstin BAIER , Heike ENKE , Dan KRAMER
IPC分类号： C12N15/82
CPC分类号： C12P7/065 , C12N15/52 , C12N15/74 , C12N15/8243 , Y02E50/17
摘要： This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds. Further provisions of the present invention include, inter alia, a method for producing the metabolically enhanced cyanobacterium and a method for producing a first chemical compound by culturing the metabolically enhanced cyanobacterium.
摘要翻译：本发明提供了一种代谢增强的蓝细菌，用于生产感兴趣的化合物，其具有至少两个编码第一生物催化剂的第一生产基因用于生产第一化合物。两个第一个生产基因之一是在第一个生产基因的第一个启动子的转录控制下，而两个第一个生产基因中的另一个在第一个生产基因的第二个启动子的转录控制下。第一启动子和第二启动子在不同条件下分别诱导，并且至少两个第一生物催化剂催化相同的化学反应。根据本发明的代谢增强的蓝细菌允许长期生产第一化合物。本发明的进一步规定尤其包括生产代谢增强的蓝细菌的方法和通过培养代谢增强的蓝细菌来生产第一化合物的方法。

3. 发明授权

US08404466B2 Metabolically enhanced photoautotrophic ethanol producing host cells, method for producing the host cells, constructs for the transformation of the host cells, and method of producing ethanol using the host cells 失效
标题翻译：代谢增强的光自养乙醇生产宿主细胞，产生宿主细胞的方法，宿主细胞转化的构建体和使用宿主细胞产生乙醇的方法
公开(公告)号：US08404466B2
公开(公告)日：2013-03-26
申请号：US13372456
申请日：2012-02-13
申请人： Kerstin Baier , Ulf Duhring , Christine Oesterhelt , Karl Ziegler , Heike Enke
发明人： Kerstin Baier , Ulf Duhring , Christine Oesterhelt , Karl Ziegler , Heike Enke
IPC分类号： C12P7/06 , C12P21/06 , C12P19/34 , C12N1/00 , C12N1/20 , C12N1/12 , C12N15/00 , C07H21/04
CPC分类号： C12P7/065 , C12N9/0006 , C12N9/1029 , C12N9/1217 , C12N9/88 , Y02E50/17
摘要： One embodiment of the invention provides a metabolically enhanced photoautotrophic, ethanol producing host cell comprising: at least two first metabolic enhancements reducing the enzymatic activity or affinity of at least two endogenous host cell enzymes involved in acetate and lactate fermentation, the first metabolic enhancements resulting in an enhanced level of biosynthesis of acetaldehyde, pyruvate, acetyl-CoA or precursors thereof compared to the respective wild type host cell, at least one second metabolic enhancement different from the first metabolic enhancement comprising an overexpressed enzyme for the formation of ethanol.
摘要翻译：本发明的一个实施方案提供了代谢增强的光自养营养型乙醇生产宿主细胞，其包含：至少两种降低与参与乙酸盐和乳酸发酵的至少两种内源性宿主细胞酶的酶活性或亲和力的第一代谢增强，所述第一代谢增强导致与相应的野生型宿主细胞相比，乙醛，丙酮酸，乙酰辅酶A或其前体的生物合成的增加水平与第一代谢增强不同的至少一种第二代谢增强包含用于形成乙醇的过表达酶。

4. 发明申请

US20120232315A1 Vapor Compression Steam Stripping 有权
标题翻译：蒸汽压缩蒸汽汽提
公开(公告)号：US20120232315A1
公开(公告)日：2012-09-13
申请号：US13481143
申请日：2012-05-25
申请人： Benjamin Moll , Edwin Malkiel , Rong Dong
发明人： Benjamin Moll , Edwin Malkiel , Rong Dong
IPC分类号： C07C29/78 , B01D3/00
CPC分类号： B01D5/003 , B01D1/2856 , B01D3/004 , B01D3/007 , B01D3/346 , B01D3/38 , B01D5/006 , B01D19/00 , B01D19/001 , B01D19/0015
摘要： The invention provides systems and methods for the pre-concentration of a target molecule from feed solution comprising a low concentration of the target molecule.
摘要翻译：本发明提供了用于从包含低浓度靶分子的进料溶液中预浓缩靶分子的系统和方法。

5. 发明申请

US20120220027A1 Magnetically Coupled System For Mixing 失效
标题翻译：用于混合的磁耦合系统
公开(公告)号：US20120220027A1
公开(公告)日：2012-08-30
申请号：US13405012
申请日：2012-02-24
申请人： Harlan Miller, III , George Meichel , Edward Legere , Edwin Malkiel , R. Paul Woods , Oliver Ashley , Joseph Katz , Jason Ward , Paul Petersen
发明人： Harlan Miller, III , George Meichel , Edward Legere , Edwin Malkiel , R. Paul Woods , Oliver Ashley , Joseph Katz , Jason Ward , Paul Petersen
IPC分类号： C12M1/02 , B01J19/18 , C12M1/42 , B01F13/08 , B01F5/12
CPC分类号： C12M27/02 , B01F13/0015 , B01F13/08 , B01F13/0827 , B01F15/00545 , C12M21/02
摘要： The invention provides a mixing system comprising a magnetically coupled drive system and a foil for cultivating algae, or cyanobacteria, in an open or enclosed vessel. The invention provides effective mixing, low energy usage, low capital expenditure, and ease of drive system component maintenance while maintaining the integrity of a sealed mixing vessel.
摘要翻译：本发明提供了一种混合系统，其包括磁耦合驱动系统和用于在开放或封闭的容器中培养藻类或蓝细菌的箔。本发明提供有效的混合，低能量使用，低资本支出以及便于驱动系统部件维护，同时保持密封混合容器的完整性。

6. 发明申请

US20120171752A1 Water/Carbonate Stripping For CO2 Capture Adsorber Regeneration And CO2 Delivery To Photoautotrophs 审中-公开
标题翻译：用于CO2捕获吸附器再生和CO2输送到光自养基的水/碳酸盐剥离
公开(公告)号：US20120171752A1
公开(公告)日：2012-07-05
申请号：US13417172
申请日：2012-03-09
申请人： Ronald CHANCE , William J. KOROS , Benjamin MCCOOL , James NOEL
发明人： Ronald CHANCE , William J. KOROS , Benjamin MCCOOL , James NOEL
IPC分类号： C12N1/20 , C12M1/12
CPC分类号： A01G7/02 , Y02C10/08 , Y02P60/24
摘要： The invention provides systems and methods for the delivery of carbon to photoautotrophs. The invention utilizes low energy regeneration of adsorbent for CO2 capture and provides for effective CO2 loading into liquids useful for photoautotroph growth and/or production of photosynthetic products, such as biofuels, via photoautotrophic culture media. The inventive system comprises a fluid/membrane/fluid contactor that provides selective transfer of molecular CO2 via a dense (non-porous) membrane from a carbonate-based CO2 snipping solution to a culture medium where the CO2 is consumed by a photoautotroph for the production of biofuels, biofuel precursors or other commercial products.
摘要翻译：本发明提供用于将碳递送到光自养体的系统和方法。本发明利用吸附剂的低能量再生来进行二氧化碳捕获，并且通过光自养营养培养基提供有效的二氧化碳负载到可用于光自养生长和/或生产光合产物（例如生物燃料）的液体中。本发明的系统包括流体/膜/流体接触器，其通过致密（非多孔）膜从碳酸盐基二氧化碳捕捉溶液向培养基提供分子CO 2的选择性转移，其中CO 2被光自养体消耗以用于生产生物燃料，生物燃料前体或其他商业产品。

7. 发明授权

US09138678B2 Membrane-augmented distillation with compression and condensation to separate solvents from water 有权
标题翻译：膜增压蒸馏，压缩和冷凝，从水中分离出溶剂
公开(公告)号：US09138678B2
公开(公告)日：2015-09-22
申请号：US13370062
申请日：2012-02-09
申请人： Yu Huang , Richard W. Baker , Benjamin McCool , Rong Dong
发明人： Yu Huang , Richard W. Baker , Benjamin McCool , Rong Dong
IPC分类号： B01D3/14 , B01D53/22 , B01D61/36 , B01D1/28 , C07C29/76 , C07C31/08 , B01D71/32 , B01D71/44
CPC分类号： B01D53/228 , B01D3/145 , B01D61/362 , B01D71/32 , B01D71/44 , B01D2311/2669 , C07C29/76 , C07C31/08
摘要： Disclosed herein are processes for removing water from organic solvents, such as ethanol. The processes include distillation in two columns operated at sequentially higher pressure, followed by treatment of the overhead vapor by one or two membrane separation steps.
摘要翻译：本文公开了从有机溶剂如乙醇中除去水的方法。这些方法包括在连续较高压力下运行的两个塔中进行蒸馏，然后通过一个或两个膜分离步骤处理塔顶蒸气。

8. 发明申请

US20120137727A1 Membrane-Augmented Distillation with Compression and Condensation to Separate Solvents from Water 有权
标题翻译：膜增压蒸馏与压缩和冷凝分离溶剂与水
公开(公告)号：US20120137727A1
公开(公告)日：2012-06-07
申请号：US13370062
申请日：2012-02-09
申请人： Yu Huang , Richard W. Baker , Benjamin McCool , Rong Dong
发明人： Yu Huang , Richard W. Baker , Benjamin McCool , Rong Dong
IPC分类号： F25J3/08
CPC分类号： B01D53/228 , B01D3/145 , B01D61/362 , B01D71/32 , B01D71/44 , B01D2311/2669 , C07C29/76 , C07C31/08
摘要： Disclosed herein are processes for removing water from organic solvents, such as ethanol. The processes include distillation in two columns operated at sequentially higher pressure, followed by treatment of the overhead vapor by one or two membrane separation steps.
摘要翻译：本文公开了从有机溶剂如乙醇中除去水的方法。这些方法包括在连续较高压力下运行的两个塔中进行蒸馏，然后通过一个或两个膜分离步骤处理塔顶蒸气。

9. 发明申请

US20160024458A1 Process For Inoculating Closed Photobioreactors With Cyanobacteria 审中-公开
标题翻译：用蓝藻接种封闭式光生物反应器的方法
公开(公告)号：US20160024458A1
公开(公告)日：2016-01-28
申请号：US14774459
申请日：2014-03-15
申请人： ALGENOL BIOFUELS INC.
发明人： Laura BELICKA , Jessica BLANKS , Hayley FLORES , Isaac HOLOWELL , Frank JOCHEM , George MEICHEL , Harlan MILLER, III , Jesse Phillips-Kress , Brendan SCOTT , Christophe VASSEUR
IPC分类号： C12N1/12 , C12P7/06
CPC分类号： C12N1/12 , C12M21/02 , C12M21/12 , C12M23/58 , C12N1/20 , C12P7/065 , C12P7/14 , Y02E50/17
摘要： Methods of inoculum scale-up of cyanobacteria to commercial-scale closed photobioreactors for the production of a target molecule such as ethanol.
摘要翻译：将商业规模封闭式光生物反应器的蓝细菌接种放大生产目标分子如乙醇的方法。

10. 发明申请

US20150184221A1 METHODS FOR ANALYSES OF CYANOBACTERIAL RESTRICTION ENDONUCLEASES 有权
标题翻译： CYANOBACTERIAL限制性内切酶分析方法
公开(公告)号：US20150184221A1
公开(公告)日：2015-07-02
申请号：US14144361
申请日：2013-12-30
申请人： Algenol Biofuels Inc.
发明人： Marc Meidlinger
IPC分类号： C12Q1/44 , C12N15/82
CPC分类号： C12Q1/44 , G01N2333/922
摘要： Methods are provided for analyses of restriction endonucleases.
摘要翻译：提供了限制性内切酶的分析方法。

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