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1. 发明申请

US20090075826A1 Methods and apparatuses for sorting objects in forensic DNA analysis and medical diagnostics 失效
标题翻译：在法医DNA分析和医学诊断中排序物体的方法和设备
公开(公告)号：US20090075826A1
公开(公告)日：2009-03-19
申请号：US12232165
申请日：2008-09-11
申请人： Tania Chakrabarty
发明人： Tania Chakrabarty
IPC分类号： C40B20/08 , C40B60/10
CPC分类号： B01L3/502761 , B01L7/52 , B01L2200/0652 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2400/0454 , G01N35/00029 , G01N2001/007
摘要： The present invention relates to an apparatus and method of sorting objects and identifying the objects in a forensics sample, including using holographic optical trapping to sort objects from contaminants, and performing (single cell) PCR-based STR analysis on the objects to determine their identification. In addition, the chip used as a support for sorting the objects can also be used for performing single cell PCR-based STR analysis. In another embodiment, a microfluidics chip is used to stream the sample and sort the objects, before single cell PCR-based STR analysis is performed. The chip used for sorting utilizing HOT in the absence or presence of microfluidic streaming and sorting can also be the same as that used for the single cell PCR-based STR analysis.
摘要翻译：本发明涉及一种用于对物体进行排序并识别取证样本中的物体的装置和方法，包括使用全息光学捕集对来自污染物的物体进行分类，以及对对象执行（单细胞）基于PCR的STR分析以确定其识别。另外，作为用于分类对象的支持的芯片也可以用于进行基于单细胞PCR的STR分析。在另一个实施方案中，在进行基于单细胞PCR的STR分析之前，使用微流体芯片来流式化样品并对对象进行分类。在不存在或存在微流体流和分选的情况下用于使用HOT分选的芯片也可以与用于单细胞基于PCR的STR分析相同。

2. 发明授权

US07466883B2 Optical switches and routers and optical filters 失效
标题翻译：光开关和路由器和光滤波器
公开(公告)号：US07466883B2
公开(公告)日：2008-12-16
申请号：US11403941
申请日：2006-04-14
申请人： Kenneth Bradley , Ward Lopes
发明人： Kenneth Bradley , Ward Lopes
IPC分类号： G02B6/26 , G02B6/32
CPC分类号： G02B6/12007 , G02B6/29341 , G02B6/29343 , G02F1/3132 , G02F2203/15
摘要： Our invention relates generally to an optical switch (31) and an optical router (10) to rapidly route signals from particular channels (22, 24) within an optical band by using optical switches (20) which utilize a controlled whispering gallery mode (WGM) resonance of dielectric microspheres (S1, S2, S3) to optically switch signals. Another invention relates to optical filters which use a WGM resonate structure (150) to isolate and switch specific optical signals between waveguides (F1, F2). In other inventions, the filter (100) is switched “on/off” by signal loss within a WGM resonate structure (150) which disrupts the WGM resonance; the filter (100) isolates and switches a specific wavelength signal from among a group of signals of different wavelengths; and is switched “off” by adjusting the index of refraction of the resonate structure to become substantially similar to the index of refraction of the surrounding medium.
摘要翻译：本发明一般涉及光开关（31）和光路由器（10），用于通过使用光开关（20）来快速地从光频带内的特定通道（22,24）传送信号，所述光开关利用受控的耳语画廊模式（WGM ）电介质微球的共振（S1，S2，S3）来光学切换信号。另一发明涉及使用WGM谐振结构（150）来隔离和切换波导（F1，F2）之间的特定光信号的滤光器。在其它发明中，滤波器（100）通过在WGM共振结构（150）内的信号损失被切换为“开/关”，这破坏了WGM谐振; 滤波器（100）从不同波长的一组信号中隔离并切换特定的波长信号; 并且通过调节谐振结构的折射率而变得基本上类似于周围介质的折射率而被切换为“关闭”。

3. 发明授权

US07449679B2 System and method for manipulating and processing materials using holographic optical trapping 失效
标题翻译：使用全息光学捕获来操纵和处理材料的系统和方法
公开(公告)号：US07449679B2
公开(公告)日：2008-11-11
申请号：US11079533
申请日：2005-03-15
申请人： Joseph Plewa , Evan Tanner , Daniel Mueth , Lewis Gruber , Kenneth Bradley
发明人： Joseph Plewa , Evan Tanner , Daniel Mueth , Lewis Gruber , Kenneth Bradley
IPC分类号： H05H3/02 , C12N13/00
CPC分类号： B82Y20/00 , B82Y10/00 , G03H1/08 , G03H1/2294 , G03H2001/0077 , G03H2225/32
摘要： A method and apparatus for manipulating particles (micro, nano, and pico) having one or more characteristics with an optical trap formed by modulating a laser beam with a Diffractive Optical Element (DOE). At least one characteristic of the material is selected; and a laser beam having a selected wavelength corresponding to the at least one selected characteristic of the material is generated. Values of the DOE are calculated corresponding to the at least one selected characteristic of the material. The beam and the DOE are modulated to produce a holographic optical trap having properties corresponding to the at least one selected characteristic; the trap is focused to a beam focus or selected spot size; and the beam focus is located near a particle location for trapping the particle therein.
摘要翻译：一种用于通过用衍射光学元件（DOE）调制激光束而形成的光阱来操纵具有一个或多个特性的微粒（微，纳米和微微）的方法和装置。选择材料的至少一种特性; 并且产生具有与材料的至少一个所选特征对应的选定波长的激光束。根据材料的至少一个所选特征计算DOE的值。光束和DOE被调制以产生具有对应于至少一个所选特征的性质的全息光阱; 陷阱聚焦到光束焦点或选定的光斑尺寸; 并且束聚焦位于颗粒位置附近以将颗粒捕获在其中。

4. 发明授权

US07355696B2 Method and apparatus for sorting cells 有权
标题翻译：用于分选细胞的方法和装置
公开(公告)号：US07355696B2
公开(公告)日：2008-04-08
申请号：US11046896
申请日：2005-02-01
申请人： Daniel Mueth , Amy L. Anderson , Christopher R. Knutson , Joseph Plewa
发明人： Daniel Mueth , Amy L. Anderson , Christopher R. Knutson , Joseph Plewa
IPC分类号： G01N21/01
CPC分类号： G01N21/6428 , C12M47/04 , G01N15/1404 , G01N15/1459 , G01N2015/1409 , G01N2015/1411 , G01N2015/149
摘要： Apparatus for sorting and orienting sperm cells has a pair or walls in confronting relationship forming a flow chamber having inlet, a downstream outlet, and intermediate detector region. The inlet receives first and second spaced apart streams of input fluid and a third stream of sample fluid containing the cells to be sorted. The first and second streams have respective flow rates relative to third stream, such that the third stream is constricted forming a relatively narrow sample stream, so that the cells are oriented parallel to the walls. A detector detect desired cells and a sorter downstream of the detector for sorting the desired cells from the stream.
摘要翻译：用于分选和定向精子细胞的装置具有一对或多个相对关系的壁，形成具有入口，下游出口和中间检测器区域的流动室。入口接收第一和第二间隔开的输入流体流和含有待分选细胞的第三流样品流体。第一和第二流相对于第三流具有相应的流速，使得第三流收缩形成相对窄的样品流，使得电池平行于壁取向。检测器检测期望的细胞和检测器下游的分选器，用于从流中分选所需的细胞。

5. 发明申请

US20070029257A1 Multiple laminar flow-based particle and cellular separation with laser steering 有权
标题翻译：多层次流动粒子与细胞分离与激光转向
公开(公告)号：US20070029257A1
公开(公告)日：2007-02-08
申请号：US11543773
申请日：2006-10-06
申请人： Daniel Mueth , Joseph Plewa , Jessica Shireman , Amy Anderson , Lewis Gruber , Neil Rosenbaum
发明人： Daniel Mueth , Joseph Plewa , Jessica Shireman , Amy Anderson , Lewis Gruber , Neil Rosenbaum
IPC分类号： B01D61/00
CPC分类号： A61M1/3681 , A61M1/36 , A61M1/3693 , A61M1/38 , A61M2206/11 , B01L3/502761 , B03C1/28 , B03C5/005 , B03C2201/02 , B03C2201/18 , G01N30/0005 , G03H1/08 , G03H1/2294 , G03H2001/0077 , G03H2001/085 , Y10T436/25375
摘要： The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.
摘要翻译：本发明提供了一种用于分离血液和其他类型的细胞组分的方法，装置和系统，并且可以与全息光学捕获操作或其他形式的光学镊子组合。示例性方法之一包括提供具有多个血液成分的第一流; 提供第二流; 使第一流与第二流接触以提供第一分离区; 以及将所述多个血液成分的第一血细胞成分差异沉积到所述第二流中，同时在所述第一流中同时维持所述多个血液成分的第二血细胞成分。然后，具有第一血细胞成分的第二流与具有第二血细胞成分的第一流有差别地移除。全息光学捕集器也可以与各种流动结合使用，以将选定的组分从一个流移动到另一个流，作为分离阶段的一部分或除了分离阶段之外。

6. 发明申请

US20120202225A1 METHODS AND DEVICES FOR IMMUNODIAGNOSTIC APPLICATIONS 审中-公开
标题翻译：用于免疫组化应用的方法和装置
公开(公告)号：US20120202225A1
公开(公告)日：2012-08-09
申请号：US13364279
申请日：2012-02-01
申请人： Christopher R. Knutson , Christopher F. Grant , Timothy R. Kline , Derek D. Doorneweerd , Sridevi Kurella , Daniel M. Mueth , Matthew K. Runyon , Haojun Fu , Sergio O. Guevara
发明人： Christopher R. Knutson , Christopher F. Grant , Timothy R. Kline , Derek D. Doorneweerd , Sridevi Kurella , Daniel M. Mueth , Matthew K. Runyon , Haojun Fu , Sergio O. Guevara
IPC分类号： G01N33/53 , C12M1/34 , G01N33/561 , G01N33/563 , G01N33/558
CPC分类号： G01N33/80 , B04B5/04 , G01N21/07
摘要： Methods and devices for evaluating a sample from a subject for detecting a target red blood cell protein or antibody are disclosed. In one embodiment, optimized antibody screening methods and devices significantly reduce the level of non-specific binding to a surface (e.g., a test surface bound with a red blood cell preparation), thus allowing for more efficient detection and reduced test time. In other embodiments, the invention provides methods and devices for target capturing that include a substantially planar surface, optionally having an optimized angle, for capture. Alternative solid phase geometries for capture are disclosed. Optimized methods for cell deposition are also disclosed. Thus, optimized methods, devices, kits, assays for evaluating a sample are disclosed.
摘要翻译：公开了用于评估来自受试者的用于检测靶红细胞蛋白或抗体的样品的方法和装置。在一个实施方案中，优化的抗体筛选方法和装置显着降低与表面（例如与红细胞制剂结合的测试表面）的非特异性结合的水平，从而允许更有效的检测和减少的测试时间。在其他实施例中，本发明提供了用于目标捕获的方法和装置，其包括用于捕获的可选地具有优化角度的基本平坦的表面。公开了用于捕获的替代固相几何形状。还公开了细胞沉积的优化方法。因此，公开了用于评估样品的优化方法，装置，试剂盒测定。

7. 发明申请

US20120028848A1 Binding method and apparatus for sorting objects 审中-公开
标题翻译：用于排序物体的装订方法和装置
公开(公告)号：US20120028848A1
公开(公告)日：2012-02-02
申请号：US13200940
申请日：2011-10-05
申请人： Tania Chakrabarty
发明人： Tania Chakrabarty
IPC分类号： C12M1/40 , C40B60/12
CPC分类号： G01N33/5091 , G01N33/56966 , G01N2800/367
摘要： The present invention relates to a method and apparatus of sorting objects including, providing a sample having wanted objects and unwanted objects; coating a surface of a sample holder with an antibody; placing an eluted sample on the sample holder; binding an antigen in the wanted objects with the antibody on the surface of the sample holder to sort the objects into wanted objects and unwanted objects; separating the wanted objects; and performing PCR-based STR analysis on the wanted objects. In one embodiment, holographic optical trapping is used to further sort the wanted objects. In other embodiments, the wanted objects are sperm and the antibody is a human sperm specific antibody, and the PCR is single cell PCR-based STR analysis. In still other embodiments, the binding is direct or indirect, ligands are used to bind to object-specific organomolecules, and protein A or protein G are used to bind the antibody.
摘要翻译：本发明涉及一种排序物品的方法和装置，包括：提供具有所需物品和不需要物品的样品; 用抗体涂覆样品架的表面; 将洗脱样品置于样品架上; 将所需物体中的抗原与样品架表面上的抗体结合，将物体分类成有用物体和不需要的物体; 分离所需物体; 并对所需物体进行基于PCR的STR分析。在一个实施例中，全息光学捕获用于进一步分类所需物体。在其他实施方案中，所需物体是精子，抗体是人精子特异性抗体，PCR是基于单细胞PCR的STR分析。在其它实施方案中，结合是直接或间接的，配体用于结合对象特异性有机分子，并且蛋白A或蛋白G用于结合抗体。

8. 发明申请

US20110091932A1 Automated extraction and purification of samples using optical tweezers 审中-公开
公开(公告)号：US20110091932A1
公开(公告)日：2011-04-21
申请号：US12926888
申请日：2010-12-15
申请人： Joseph S. Plewa , Evan B. Tanner , Daniel M. Mueth , Crystal J. Duke , Kevin J. Ehrenreich
发明人： Joseph S. Plewa , Evan B. Tanner , Daniel M. Mueth , Crystal J. Duke , Kevin J. Ehrenreich
IPC分类号： C12Q1/04 , C12Q1/02
CPC分类号： G02B21/32 , B01L3/502761 , G01N1/34 , G01N15/1463 , G01N2001/007 , G01N2015/1454 , G01N2015/149 , G02B5/32 , Y10T436/25375
摘要： An apparatus and method for purifying a forensic sample using an automated extraction and purification system includes optical tweezers; an input channel through which the sample is introduced; a chamber which receives the sample from the input channel; a collection channel through which selected particles of the sample are removed; and an output through which unselected particles of the sample are removed. At least one buffer input channel is provided. The input channel may allow sedimentation of the sample into the chamber by gravity. In another arrangement, the system includes an optical trapping system; a first channel through which the sample is introduced; a second channel through which buffer is introduced; wherein the optical tweezers are used to move selected particles of the sample from the first channel to the second channel. The selected particles may be sperm. The optical tweezers preferably utilize holographic optical trapping.

9. 发明授权

US07699767B2 Multiple laminar flow-based particle and cellular separation with laser steering 有权
公开(公告)号：US07699767B2
公开(公告)日：2010-04-20
申请号：US12213109
申请日：2008-06-13
申请人： Daniel Mueth , Joseph Plewa , Jessica Shireman , Amy Anderson , Lewis Gruber , Neil Harris Rosenbaum
发明人： Daniel Mueth , Joseph Plewa , Jessica Shireman , Amy Anderson , Lewis Gruber , Neil Harris Rosenbaum
IPC分类号： B04B7/08
CPC分类号： G03H1/0005 , A61L2/08 , B01D21/0009 , B01D21/01 , B01D21/262 , B01L3/502761 , B01L2200/0652 , B01L2200/0668 , B01L2300/0864 , B01L2400/0454 , B03C1/00 , B03C1/002 , B03C1/288 , B03C5/005 , B04B13/00 , G01N15/0205 , G01N21/6486 , G01N27/447 , G01N27/44756 , G01N33/487 , G01N33/5091 , G01N2015/0053 , G01N2201/061 , G01N2458/00 , G02B21/32 , G03H1/08 , G03H1/2294 , G03H2001/0077 , G03H2001/085 , G03H2225/32 , G21K5/08 , H05H3/04
摘要： The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

10. 发明授权

US07545491B2 Method and apparatus for sorting cells 有权
标题翻译：用于分选细胞的方法和装置
公开(公告)号：US07545491B2
公开(公告)日：2009-06-09
申请号：US12071020
申请日：2008-02-14
申请人： Daniel Mueth , Amy Anderson , Christopher Knutson , Joseph Plewa
发明人： Daniel Mueth , Amy Anderson , Christopher Knutson , Joseph Plewa
IPC分类号： G01N21/47 , G01N21/01
CPC分类号： G01N21/6428 , C12M47/04 , G01N15/1404 , G01N15/1459 , G01N2015/1409 , G01N2015/1411 , G01N2015/149
摘要： Apparatus for sorting and orienting sperm cells has a pair or walls in confronting relationship forming a flow chamber having inlet, a downstream outlet, and intermediate detector region. The inlet receives first and second spaced apart streams of input fluid and a third stream of sample fluid containing the cells to be sorted. The first and second streams have respective flow rates relative to third stream, such that the third stream is constricted forming a relatively narrow sample stream, so that the cells are oriented parallel to the walls. A detector detects desired cells and a sorter downstream of the detector for sorting the desired cells from the stream.
摘要翻译：用于分选和定向精子细胞的装置具有一对或多个相对关系的壁，形成具有入口，下游出口和中间检测器区域的流动室。入口接收第一和第二间隔开的输入流体流和含有待分选细胞的第三流样品流体。第一和第二流相对于第三流具有相应的流速，使得第三流收缩形成相对窄的样品流，使得电池平行于壁取向。检测器检测期望的细胞和检测器下游的分选器，用于从流中分选所需的细胞。

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