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81. 发明申请

US20160024484A1 GENETICALLY ENGINEERED AND ACID-RESISTANT YEAST CELL WITH ENHANCED ACTIVITY OF RADIATION SENSITIVITY COMPLEMENTING KINASE AND METHOD OF PRODUCING LACTATE BY USING THE YEAST CELL 有权
标题翻译：具有增强活性的基因工程和抗酸性YEAST细胞，其具有完整的激酶的活性和使用YEAST细胞产生LACTATE的方法
公开(公告)号：US20160024484A1
公开(公告)日：2016-01-28
申请号：US14807608
申请日：2015-07-23
申请人： Samsung Electronics Co., Ltd.
发明人： Huisub Lim , Sungsoo Kim , Jiyoon Song , Soyoung Lee , Juyoung Lee , Kwangmyung Cho
IPC分类号： C12N9/12 , C12P7/56 , C12N15/81
CPC分类号： C12N9/1205 , C07K14/39 , C07K14/395 , C12N9/12 , C12N15/81 , C12P7/56 , C12Y207/11001
摘要： Provided is an acid-resistant yeast cell that is genetically engineered to enhance activity of a radiation sensitivity complementing kinase, and a method of producing lactate by using the yeast cell.
摘要翻译：提供了遗传工程化以增强放射敏感性互补激酶活性的耐酸酵母细胞，以及通过使用酵母细胞产生乳酸盐的方法。

82. 发明授权

US09234219B2 Method for producing L-lactic acid by lactic acid bacterium under presence of pentose and cellooligosaccharides 有权
标题翻译：乳酸菌在戊糖和纤维寡糖存在下生产L-乳酸的方法
公开(公告)号：US09234219B2
公开(公告)日：2016-01-12
申请号：US13810426
申请日：2011-07-15
申请人： Kenji Sonomoto , Takeshi Zendo
发明人： Kenji Sonomoto , Takeshi Zendo
IPC分类号： C12P7/56 , C12P7/62 , C12R1/46 , C12N1/20
CPC分类号： C12P7/56 , C12N1/20 , C12P7/625 , C12R1/46
摘要： The present invention thus provides a method for producing L-lactic acid, which comprises the step of culturing a lactic acid bacterium that can produce L-lactic acid in a medium containing any one selected from the group consisting of cellobiose, cellooligosaccharides, xylose, arabinose, and glucose derived from cellulose and/or hemicellulose as a substrate to obtain L-lactic acid. In a preferred embodiment of the present invention, Enterococcus mundtii NITE BP-965 can be used.
摘要翻译：因此，本发明提供了一种L-乳酸的制造方法，其特征在于，在含有选自纤维二糖，纤维寡糖，木糖，阿拉伯糖的培养基的培养基中培养能生产L-乳酸的乳酸菌的工序，和来自纤维素和/或半纤维素的葡萄糖作为底物，得到L-乳酸。在本发明的优选实施方案中，可以使用肠球菌NITE BP-965。

83. 发明申请

US20150368679A1 METHODS OF PITCHING YEAST FOR FERMENTATION, AND RELATED METHODS OF FERMENTATION AND SYSTEMS 审中-公开
标题翻译：用于发酵的方法，以及发酵和系统的相关方法
公开(公告)号：US20150368679A1
公开(公告)日：2015-12-24
申请号：US14741885
申请日：2015-06-17
申请人： POET RESEARCH, INC.
发明人： Neelakantam V. Narendranath , Stephen M. Lewis , Neil D. Anderson
IPC分类号： C12P7/56 , C12P7/54 , C12M1/00 , C12P7/10
CPC分类号： C12M29/00 , C12M23/58 , C12N1/16 , C12N1/22 , C12P7/14 , Y02E50/16
摘要： The present invention relates to methods and systems of pitching yeast to fermentation reactors. More particularly, the present invention involves pitching yeast from one fermentation tank to at least one additional fermentation tank. Advantageously, yeast can be continuously pitched from fermentor to fermentor for as long as practically desirable.
摘要翻译：本发明涉及将酵母发酵发酵反应器的方法和系统。更具体地，本发明涉及将酵母从一个发酵罐投入到至少一个另外的发酵罐中。有利地，酵母可以从发酵罐到发酵罐连续地保持长达实际期望的长度。

84. 发明申请

US20150329884A1 PROCESS OF PRODUCING A LACTATE FERMENT 审中-公开
标题翻译：生产羊毛发酵的过程
公开(公告)号：US20150329884A1
公开(公告)日：2015-11-19
申请号：US14760977
申请日：2014-01-15
申请人： PURAC BIOCHEM B.V.
发明人： Brenda Marja DIERDORP-ANDREAE , Jasper MEIJER , Scott Allan MCELMURY , Johannes Cornelius Adrianus BLONK , Lansen Court MOREHOUSE
IPC分类号： C12P7/56 , A23C9/123
摘要： The present invention relates to fermentation processes. The objective of the invention was to provide methods for producing ferments and formulating such ferments into preservatives. It was in particular an object of the present invention to provide improved processes for producing lactic acid containing ferments by simple and efficient (batch) culturing and allowing for simple down-stream processing either into a liquid, semi-liquid or a dry powder product, having sufficient stability, good handling properties and satisfactory organoleptic properties. Such processes are provided, as well as the products that can be obtained with it and their use as food preservative.
摘要翻译：本发明涉及发酵方法。本发明的目的是提供生产发酵剂并将这些发酵剂配制成防腐剂的方法。特别是本发明的目的是提供通过简单且有效的（分批）培养生产含乳酸的改良方法，并允许简单的下游加工成液体，半液体或干粉产品，具有足够的稳定性，良好的操作性能和令人满意的感官特性。提供这样的方法，以及可以获得的产品及其作为食品防腐剂的用途。

85. 发明申请

US20150315615A1 BATCH FEED PROCESS FOR FERMENTING SUGARS 审中-公开
标题翻译：用于发酵糖的批料进料
公开(公告)号：US20150315615A1
公开(公告)日：2015-11-05
申请号：US14650976
申请日：2013-12-10
申请人： CARGILL, INCORPORATED
发明人： Ravi GOKARN , Mattew PARSONS , Derran WALCKER , Joseph SPENCER
IPC分类号： C12P7/56
CPC分类号： C12P7/56 , C12P7/40
摘要： A batch fermentation process ferments a starch hydrolysate containing 80-98 weight percent of glucose based on total carbohydrate and 0.3-5% weight percent of isomaltose based on total carbohydrate to a fermentation product. A fermentation broth is formed containing a first portion of a total amount of the starch hydrolysate so that the fermentation broth has an initial glucose concentration of at least about 50 g/L. Fermentation is carried out until the fermentation broth contains 30 g/L or less of glucose. An effective amount of at least one active enzyme that converts isomaltose into glucose is adding to the fermentation broth. Then the remaining portion of the total amount of starch hydrolysate is fed into the fermentation broth to maintain a glucose concentration of from about 5 to about 15 g/L in the fermentation broth throughout the feeding step. The final fermentation broth containing the fermentation product is then produced.
摘要翻译：分批发酵方法将基于总碳水化合物含有80-98重量％葡萄糖的淀粉水解物和基于总碳水化合物的0.3-5重量％的异麦芽糖发酵成发酵产物。形成含有总量为淀粉水解产物的第一部分的发酵液，使得发酵液的初始葡萄糖浓度为至少约50g / L。发酵直到发酵液含有30g / L以下的葡萄糖。将有效量的至少一种将异麦芽糖转化成葡萄糖的活性酶是向发酵液中加入。然后将淀粉水解产物的总量的剩余部分进料到发酵液中，以在整个进料步骤中在发酵液中维持约5至约15g / L的葡萄糖浓度。然后生产含有发酵产物的最终发酵液。

86. 发明申请

US20150299674A1 Polypeptides Having Laccase Activity and Polynucleotides Encoding Same 有权
标题翻译：具有漆酶活性的多肽和编码相同的多核苷酸
公开(公告)号：US20150299674A1
公开(公告)日：2015-10-22
申请号：US14363490
申请日：2012-12-14
申请人： Novozymes Inc.
发明人： Ye Liu , Lan Tang , Junxin Duan , Yu Zhang
IPC分类号： C12N9/02 , C12P19/12 , C12P7/16 , C12P7/10 , C12P7/20 , C12P7/04 , C12P7/02 , C12P7/18 , C12P5/02 , C12P5/00 , C12P13/20 , C12P13/14 , C12P13/00 , C12P13/08 , C12P13/06 , C12P3/00 , C12P7/26 , C12P7/28 , C12P7/40 , C12P7/54 , C12P7/44 , C12P17/04 , C12P7/48 , C12P7/58 , C12P7/46 , C12P7/42 , C12P7/56 , C12P7/50 , C12P7/52 , C11D3/386 , C12P19/02
CPC分类号： C12N9/0061 , C11D3/38636 , C11D3/38645 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2203/00 , Y02E50/16
摘要： The present invention relates to isolated polypeptides having laccase activity and polynucleotides encoding the polypeptides and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
摘要翻译：本发明涉及具有漆酶活性的分离多肽和编码多肽的多核苷酸和编码多肽的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

87. 发明授权

US09157102B2 Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance 有权
标题翻译： NADH依赖性氧化还原酶（fucO）的过表达用于增加糠醛或5-羟甲基糠醛耐受性
公开(公告)号：US09157102B2
公开(公告)日：2015-10-13
申请号：US14008015
申请日：2012-03-29
申请人： Elliot N. Miller , Xueli Zhang , Lorraine P. Yomano , Xuan Wang , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
发明人： Elliot N. Miller , Xueli Zhang , Lorraine P. Yomano , Xuan Wang , Keelnatham T. Shanmugam , Lonnie O'Neal Ingram
IPC分类号： C12P7/56 , C12N9/04 , C12P7/06 , C12P7/10 , C12P7/46
CPC分类号： C12P7/56 , C12N9/0006 , C12P7/06 , C12P7/065 , C12P7/10 , C12P7/46 , C12Y101/01202 , Y02E50/16 , Y02E50/17
摘要： The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.
摘要翻译：本发明涉及NADH依赖性丙二醇氧化还原酶（FucO）可以减少糠醛的发现。这允许用于改善用于产生所需产物的细菌和/或酵母细胞中的糠醛耐受性的新方法。因此，提供了对糠醛和5-羟甲基糠醛（5-HMF）具有增加的耐受性的新型生物催化剂（细菌，真菌或酵母细胞），其是制备和使用这些生物催化剂以制备所需产物的方法。

88. 发明申请

US20150240272A1 LACTATE PRODUCTION PROCESS 审中-公开
标题翻译： LACTATE生产工艺
公开(公告)号：US20150240272A1
公开(公告)日：2015-08-27
申请号：US14429245
申请日：2013-09-19
申请人： PLAXICA LIMITED
发明人： Stephen Alexander Calder Smith , James Andrew Surman
IPC分类号： C12P7/62 , C08G63/06 , C12P7/56
CPC分类号： C12P7/625 , C07C67/333 , C08G63/06 , C12P7/56 , C12P7/62 , C12P41/004 , C07C69/67
摘要： A process for producing a compound of formula (I) is provided. The process comprises reacting a mixture comprising a compound of formula (IIA) and a compound of formula (IIB) with an alkyl alcohol in the presence of a Candida antarctica lipase B enzyme to produce a product comprising the compound of formula (I) and the compound of formula (IIB); wherein R1 is C1 to C6 alkyl; and wherein R2 is II or C1 to C12 alkyl. Also provided are processes for the production of R-lactic acid, oligomeric R-lactic acid, R,R-lactide, poly-R-lactic acid, alkyl S-lactate, S-lactic acid, oligomeric S-lactic acid, S,S-lactide, poly S-lactic acid and stereocomplex polylactic acid.
摘要翻译：提供了制备式（I）化合物的方法。该方法包括在南极假丝酵母脂肪酶B酶的存在下使包含式（IIA）化合物和式（IIB）化合物与烷基醇的混合物反应，以产生包含式（I）化合物和式（IIB）化合物; 其中R1是C1-C6烷基; 并且其中R 2是II或C 1至C 12烷基。还提供了制备R-乳酸，低聚R-乳酸，R，R-丙交酯，聚R-乳酸，S-乳酸烷基酯，S-乳酸，低聚S-乳酸，S， S-丙交酯，聚S-乳酸和立体络合物聚乳酸。

89. 发明申请

US20150240270A1 PRODUCTION OF ORGANIC ACIDS BY FERMENTATION AT LOW PH 审中-公开
标题翻译：通过低PH发酵生产有机酸
公开(公告)号：US20150240270A1
公开(公告)日：2015-08-27
申请号：US14428202
申请日：2013-09-13
申请人： MYRIANT CORPORATION
发明人： R. Rogers Yocum , Sudhanshu Dole , Janice G. Pero
IPC分类号： C12P7/46 , C12P7/56
CPC分类号： C12P7/46 , C12N1/14 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1025 , C12N9/12 , C12N9/88 , C12N9/93 , C12N15/52 , C12N15/81 , C12P7/56
摘要： This invention relates to the biosynthesis of organic acids in genetically modified microorganisms. More specifically, this invention provides genetically modified microorganisms that are particularly tolerant to organic acids at low pH and are capable of producing organic acids by fermentation at low pH.
摘要翻译：本发明涉及转基因微生物中有机酸的生物合成。更具体地，本发明提供了在低pH下特别耐受有机酸的遗传修饰的微生物，并且能够通过在低pH下发酵来产生有机酸。

90. 发明申请

US20150225752A1 ACID RESISTANT YEAST CELL AND USE THEREOF 有权
标题翻译：耐药性YEAST细胞及其用途
公开(公告)号：US20150225752A1
公开(公告)日：2015-08-13
申请号：US14622315
申请日：2015-02-13
申请人： Samsung Electronics Co., Ltd.
发明人： Huisub Lim , Changduk Kang , Jiyoon Song , Seunghyun Lee , Kwangmyung Cho
IPC分类号： C12P7/56 , C12N15/01
CPC分类号： C12P7/56 , C12N15/01 , C12R1/865
摘要： Saccharomyces cerevisiae having acid resistance at a pH of about 2.0 to about 5.0, a method of preparing the Saccharomyces cerevisiae, and a method of producing lactate.
摘要翻译：在约2.0至约5.0的pH下具有耐酸性的酿酒酵母，制备酿酒酵母的方法，以及产生乳酸的方法。

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