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81. 发明申请

US20030229908A1 Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions 有权
标题翻译：通过交叉种核移植产生的胚胎或干细胞系以及通过供体细胞遗传改变或通过组织培养条件增强胚胎发育的方法
公开(公告)号：US20030229908A1
公开(公告)日：2003-12-11
申请号：US10329979
申请日：2002-12-27
申请人： Advanced Cell Technology
发明人： Jose Cibelli , Michael D. West
IPC分类号： A01K067/027
CPC分类号： C12N15/873 , A01K2217/075 , A61K35/12 , A61K38/00 , C12N15/87 , C12N15/877 , C12N2510/00 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of differentiated donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation. Also, methods for improving nuclear transfer efficiency by genetically altering donor cells to inhibit apoptosis, select for a specific cell cycle and/or enhance embryonic growth and development are provided.
摘要翻译：提供了涉及将不同供体细胞核移植到与供体细胞不同的物种的去核卵母细胞中的改进的核转移方法。所得到的核转移单位可用于生产等原胚胎干细胞，特别是人原代胚胎干细胞。这些胚胎或干细胞样细胞可用于产生所需的分化细胞并用于通过同源重组引入，去除或修饰所需基因，例如在这些细胞的基因组的特定位点。这些可能含有异源基因的细胞特别适用于细胞移植治疗和体外研究细胞分化。此外，提供了通过遗传改变供体细胞以抑制细胞凋亡，选择特定细胞周期和/或增强胚胎生长和发育来提高核转移效率的方法。

82. 发明申请

US20030068818A1 Animal tissue with carbohydrate antigens compatible for human transplantation and a carbohydrate determinant selection system for homologous recombination 有权
标题翻译：具有与人类移植相容的碳水化合物抗原的动物组织和用于同源重组的碳水化合物决定簇选择系统
公开(公告)号：US20030068818A1
公开(公告)日：2003-04-10
申请号：US10105963
申请日：2002-03-21
发明人： Chris Denning , A. John Clark , J. Michael Schiff
IPC分类号： C12N005/08 , C12N005/06
CPC分类号： A01K67/0276 , A01K67/0271 , A01K2217/075 , A01K2227/105 , A01K2267/02 , A01K2267/025 , A61K2035/122 , C12N9/1051 , C12N9/1276 , C12N15/8509 , C12N15/873 , C12N2517/02 , C12N2517/04 , C12N2517/10 , C12N2800/108 , C12N2800/30
摘要： This disclosure provides a system for generating animal tissue with carbohydrate antigens that are compatible for transplantation into human patients. The tissue is inactivated homozygously for expression of null(1,3)galactosyltransferase, and comprises a transgene for null(1,2)fucosyltransferase. As a result, cell-surface N-acetyl lactosamine is not converted to the Galnull(1,3)Gal xenoantigen. Instead, it is converted to Fucnull(1,2)Gal, which is H substance, a self-antigen in humans. The tissue may also contain A or B-transferase, which will cause H substance to be converted into other ABO blood group antigens for compatibility with patients of the same blood type. This invention improves transplant compatibility of the xenograft tissue by lessening the risk of reactions resulting from xenoantigen and unconverted N-acetyl lactosamine acceptor determinants.
摘要翻译：本公开提供了一种用于产生具有与人类患者移植相容的碳水化合物抗原的动物组织的系统。组织对于α（1,3）半乳糖基转移酶的表达是纯合的，并且包含α（1,2）岩藻糖基转移酶的转基因。结果，细胞表面N-乙酰乳糖胺不转化为Galalpha（1,3）Gal异种抗原。相反，它被转化为人类中的自身抗原的Fucalpha（1,2）Gal，它是H物质。组织还可以含有A或B转移酶，这将导致H物质转化成其他ABO血型抗原，以与相同血型的患者相容。本发明通过减轻由异种抗原和未转化的N-乙酰乳糖胺受体决定簇产生的反应的风险来改善异种移植物组织的移植物相容性。

83. 发明申请

US20020142397A1 Methods for altering cell fate 审中-公开
标题翻译：改变细胞命运的方法
公开(公告)号：US20020142397A1
公开(公告)日：2002-10-03
申请号：US10015824
申请日：2001-12-10
发明人： Philippe Collas , James M. Robl
IPC分类号： C12P021/02 , C12N015/87 , C12N005/06
CPC分类号： C12N5/0696 , A61K35/12 , C12N2510/00 , C12N2510/02 , C12N2510/04 , C12N2517/04
摘要： The invention provides methods for altering the expression profile of a cell to convert the cell from one cell type to a desired cell type. These reprogrammed cells may be used in a variety of medical applications for treating a mammal in need of a particular cell type.
摘要翻译：本发明提供了用于改变细胞的表达谱以将细胞从一种细胞类型转化为所需细胞类型的方法。这些重编程细胞可以用于治疗需要特定细胞类型的哺乳动物的各种医学应用中。

84. 发明申请

US20020046410A1 Method for generating immune-compatible cells and tissues using nuclear transfer techniques 审中-公开
标题翻译：使用核转移技术产生免疫相容细胞和组织的方法
公开(公告)号：US20020046410A1
公开(公告)日：2002-04-18
申请号：US09797684
申请日：2001-03-05
发明人： Robert Lanza , Michael D. West
IPC分类号： A01K067/00 , A61K049/00
CPC分类号： C12N15/873 , A01K67/0271 , A01K2217/05 , A01K2227/101 , A01K2227/105 , A01K2267/025 , A61K49/0008 , C12N15/8771 , C12N2517/04
摘要： This invention relates to methods for making immune compatible tissues and cells for the purpose of transplantation and tissue engineering, using the techniques of nuclear transfer and cloning. Also encompassed are methods for determining the effect on immune compatibility of expressed transgenes and other genetic manipulations of the engineered cells and tissues.
摘要翻译：本发明涉及使用核转移和克隆技术制备用于移植和组织工程的免疫相容性组织和细胞的方法。还包括确定表达的转基因对工程细胞和组织的免疫相容性和其他遗传操作的影响的方法。

85. 发明授权

US06369294B1 Methods comprising apoptosis inhibitors for the generation of transgenic pigs 失效
标题翻译：包括用于产生转基因猪的凋亡抑制剂的方法
公开(公告)号：US06369294B1
公开(公告)日：2002-04-09
申请号：US09819964
申请日：2001-03-28
申请人： Jorge A. Piedrahita , Fuller W. Bazer
发明人： Jorge A. Piedrahita , Fuller W. Bazer
IPC分类号： C12N1585
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2217/075 , C12N5/0611 , C12N2500/24 , C12N2500/32 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2502/13 , C12N2510/00 , C12N2517/04 , C12N2517/10
摘要： Disclosed are methods for the isolation of primordial germ cells, culturing these cells to produce primordial germ cell-derived cell lines, methods for transforming both the primordial germ cells and the cultured cell lines, and using these transformed cells and cell lines to generate transgenic animals. The efficiency at which transgenic animals are generated by the present invention is greatly increased, thereby allowing the use of homologous recombination in producing transgenic non-rodent animal species.
摘要翻译：公开了分离原始生殖细胞，培养这些细胞以产生原始生殖细胞来源的细胞系的方法，用于转化原始生殖细胞和培养的细胞系的方法，以及使用这些转化的细胞和细胞系产生转基因动物。通过本发明产生转基因动物的效率大大增加，从而允许在产生转基因非啮齿类动物物种中使用同源重组。

86. 发明申请

US20020031670A1 GLASS-CERAMIC SUBSTRATE FOR AN INFORMATION STORAGE MEDIUM 有权
标题翻译：用于信息存储介质的玻璃陶瓷基板
公开(公告)号：US20020031670A1
公开(公告)日：2002-03-14
申请号：US09260768
申请日：1999-03-02
发明人： NAOYUKI GOTO , JUNKO ISHIOKA , YASUYUKI KAWASHIMA
IPC分类号： B32B017/06
CPC分类号： G11B5/7315 , C03C10/0027 , C12N2510/00 , C12N2517/04 , Y10S428/90
摘要： There is provided a glass-ceramic substrate for an information storage medium usable in an information storage device of the ramp loading system. The glass-ceramic substrate has Young's modulus (GPa)/specific gravity of 37 or over and includes 0 to less than 10 weight percent (expressed on oxide basis) of Al2O3. The glass-ceramic substrate has, as its predominant crystal phases, lithium disilicate (Li2O.2SiO2) and null-quartz (null-SiO2), has a coefficient of thermal expansion within a range from 65null10null7/null C. to 130null10null7/null C. wtihin a temperature range from null50null C. to null70null C. and has a surface roughness (Ra) (arithmetic mean roughness) of 9 null or below.
摘要翻译：提供了可用于斜坡加载系统的信息存储装置中的信息存储介质的玻璃陶瓷基板。玻璃陶瓷基板的杨氏模量（GPa）/比重为37以上，包含0〜小于10重量％（以氧化物为基准）的Al 2 O 3。作为其主要晶相，玻璃 - 陶瓷衬底具有二硅酸锂（Li 2 O 2·SiO 2）和α-石英（α-SiO 2），其热膨胀系数在65×10 -7 /℃至130×10 -3的范围内， 7℃，在-50℃至+ 70℃的温度范围内，并具有9或更低的表面粗糙度（Ra）（算术平均粗糙度）。

87. 发明申请

US20020012655A1 CLONED UNGULATE EMBRYOS AND ANIMALS, USE OF CELLS, TISSUES AND ORGANS THEREOF FOR TRANSPLANTATION THERAPIES INCLUDING PARKINSON'S DISEASE 无效
标题翻译：克隆发布的胚胎和动物，细胞，组织及其用于包括帕金森病的移植治疗的组织的使用
公开(公告)号：US20020012655A1
公开(公告)日：2002-01-31
申请号：US09066652
申请日：1998-04-27
发明人： STEVEN L. STICE , JOSE CIBELLI , JAMES M. ROBL
IPC分类号： A61K048/00
CPC分类号： A01K67/0275 , A01K67/02 , A01K67/027 , A01K67/0273 , A01K2217/05 , A01K2227/101 , A61K35/12 , A61K38/00 , C12N5/0603 , C12N15/8771 , C12N15/8778 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： Methods and cell lines for cloning ungulate embryos and offspring, in particular bovines and porcines, are provided. The resultant fetuses, embryos or offspring are especially useful for the expression of desired heterologous DNAs, and may be used as a source of cells or tissue for transplantation therapy for the treatment of diseases such as Parkinson's disease.
摘要翻译：提供用于克隆有蹄类胚胎和后代的方法和细胞系，特别是牛和猪。所产生的胎儿，胚胎或后代对于所需的异源DNA的表达特别有用，并且可以用作用于治疗诸如帕金森病之类疾病的移植治疗的细胞或组织来源。

88. 发明授权

US06235970B1 CICM cells and non-human mammalian embryos prepared by nuclear transfer of a proliferating differentiated cell or its nucleus 失效
标题翻译：通过增殖分化细胞或其核的核转移制备的CICM细胞和非人哺乳动物胚胎
公开(公告)号：US06235970B1
公开(公告)日：2001-05-22
申请号：US08935052
申请日：1997-09-22
申请人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce de Leon , D. Joseph Jerry
发明人： Steven L. Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce de Leon , D. Joseph Jerry
IPC分类号： C12N1500
CPC分类号： A01K67/0275 , A01K67/027 , A01K2217/05 , A01K2227/101 , A01K2227/106 , A61K35/12 , C12N5/0603 , C12N15/8771 , C12N15/8778 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
摘要翻译：提供了涉及将供体分化的细胞核移植到与供体细胞相同物种的去核卵母细胞中的改进的核转移方法。所产生的核转移单位可用于通过胎儿和后代的产生以及用于产生包括人同种异体胚胎或干细胞在内的等基因CICM细胞的基因型和转基因基因型的倍增。通过本方法促进遗传工程或转基因哺乳动物胚胎，胎儿和后代的生产，因为供体核的分化细胞源可以被遗传修饰和克隆繁殖。

89. 发明申请

US20180237799A1 SOMATIC CELL NUCLEAR TRANSFER METHODS 审中-公开
公开(公告)号：US20180237799A1
公开(公告)日：2018-08-23
申请号：US15895976
申请日：2018-02-13
申请人： New York Stem Cell Foundation, Inc.
发明人： Dietrich M. Egli
IPC分类号： C12N15/877 , C12N5/075 , C12N5/0735
CPC分类号： C12N15/8776 , C12N5/0606 , C12N5/0609 , C12N2501/065 , C12N2501/31 , C12N2501/727 , C12N2501/999 , C12N2517/04 , C12N2517/10 , C12N2760/18845
摘要： The present invention provides methods for making reconstructed diploid human oocytes comprising the diploid genome of a human somatic cell, and also methods for making human nuclear transfer embryos, human embryonic stem cells, and human differentiated cells therefrom. The present invention also provides reconstructed human oocytes, human nuclear transfer embryos, human embryonic stem cells, and differentiated cells made using such methods, as well as compositions and kits useful in performing such methods.

90. 发明申请

US20180094240A1 ARTIFICIAL OOCYTE ACTIVATION 审中-公开
公开(公告)号：US20180094240A1
公开(公告)日：2018-04-05
申请号：US15726672
申请日：2017-10-06
申请人： Kiho Lee , Randall S. Prather
发明人： Kiho Lee , Randall S. Prather
IPC分类号： C12N5/075 , C12N15/877 , A01K67/027
CPC分类号： C12N5/0609 , A01K67/0273 , A01K2227/108 , C12N15/877 , C12N15/8778 , C12N2500/14 , C12N2500/22 , C12N2501/405 , C12N2517/04 , C12N2517/10
摘要： The present invention provides novel methods for improving the efficiency of artificial activation of unfertilized mammalian oocytes by reducing the intracellular concentration of Zn2+ in the oocyte. The methods of the invention may additionally comprise a preceding step of increasing the intracellular concentration of Ca2+ in the oocyte prior to reduction of the intracellular Zn2+ concentration. The invention further provides unfertilized oocytes activated by the disclosed methods and viable mammalian animals produced from unfertilized oocytes activated by the disclosed methods.

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