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81. 发明申请

US20070300331A1 PLANT ARTIFICIAL CHROMOSOME PLATFORMS VIA TELOMERE TRUNCATION 有权
标题翻译：植物人造色素平台通过TELOMERE TRUNCATION
公开(公告)号：US20070300331A1
公开(公告)日：2007-12-27
申请号：US11746558
申请日：2007-05-09
申请人： Weichang Yu , Juan Vega , James Birchler
发明人： Weichang Yu , Juan Vega , James Birchler
IPC分类号： A01H5/00 , A01H5/10 , C07H1/00 , C07H21/04 , C12N5/04
CPC分类号： C12N15/8202 , C12N15/82
摘要： The invention provides engineered plant minichromosomes generated by telomere mediated truncation of native chromosomes. These minichromosomes are faithfully transmitted from one generation to the next and provide an ideal platform for breeding genes into desired plant varieties with out problems, such as linkage drag, associated with standard breeding methods.
摘要翻译：本发明提供由端粒介导的天然染色体截断产生的工程化植物微染色体。这些微染色体忠实地从一代传播到下一代，并提供了将基因繁殖到所需植物品种中的理想平台，其中存在与标准育种方法相关的诸如连锁阻力等问题。

82. 发明申请

US20020132350A1 Targeted genetic manipulation using Mu bacteriophage cleaved donor complex 审中-公开
标题翻译：使用Mu噬菌体切割供体复合物进行靶向遗传操作
公开(公告)号：US20020132350A1
公开(公告)日：2002-09-19
申请号：US09951856
申请日：2001-09-13
申请人： Pioneer Hi-Bred International, Inc.
发明人： Hideki Suzuki , Leszek Alexander Lyznik
IPC分类号： C12N015/74 , C12N015/00
CPC分类号： C12N15/8213 , A01K2217/05 , A01K2217/075 , C12N15/8202 , C12N15/8206 , C12N15/902
摘要： Compositions and methods for targeted genetic manipulation of an organism are provided. The compositions are novel integration vectors derived from the Mu bacteriophage comprising an active cleaved donor complex (CDC) and further comprising a targeting mechanism whereby integration of the Mu transposable cassette may be directed to a predetermined target site within a host organism's genome. These integration vectors comprise a Mu transposable cassette and one or more navigator elements that direct targeted insertion of the CDC. Methods of the invention utilize the integration vectors of the invention to insert the Mu transposable cassette into a target site of an organism's genome. This insertion occurs in the absence of the MuB accessory protein. The methods are useful for modulating activity of known genes and for targeting integration of nucleotide sequences of interest into a specific location of an organism's genome. Accordingly, the methods may also be used to create gene disruptions and knockouts.
摘要翻译：提供了一种用于有机体靶向遗传操作的组合物和方法。所述组合物是衍生自包含活性切割供体复合物（CDC）的Mu噬菌体的新型整合载体，并且还包含靶向机制，由此可将Mu转座盒的整合导向宿主生物体基因组内的预定靶位点。这些整合载体包含Mu转座盒和一个或多个引导CDC的靶向插入的导航器元件。本发明的方法利用本发明的整合载体将Mu转座盒插入生物体基因组的靶位点。该插入发生在没有MuB辅助蛋白的情况下。该方法可用于调节已知基因的活性并靶向将感兴趣的核苷酸序列整合到生物体基因组的特定位置。因此，该方法也可用于产生基因破坏和敲除。

83. 发明授权

US5416010A Olpidium zoospores as vectors of recombinant DNA to plants 失效
标题翻译：橄榄球藻作为重组DNA向植物的载体
公开(公告)号：US5416010A
公开(公告)日：1995-05-16
申请号：US74902
申请日：1993-06-10
申请人： Willem G. Langenberg , Lingyu Zhang
发明人： Willem G. Langenberg , Lingyu Zhang
IPC分类号： C12N15/82 , C12N15/00 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8202
摘要： A method is provided for introducing a foreign gene into a plant cell by means of an Olpidium zoospore vector having associated with it a reassembled nucleoprotein complex comprising the foreign gene and reassociated coat protein of a zoospore-transmissable virus. The plant cell is contacted with the zoospore under conditions suitable for transmission of the nucleoprotein complex into the cell. The method can be conducted under nonsterile conditions, is applicable to all nucleic acids regardless of size, and is useful for introducing foreign genes into cells of both monocots and dicots.
摘要翻译：提供了一种通过与其相关的具有与其重组的核蛋白复合物的Olpidium zoospore载体将外来基因导入植物细胞的方法，所述重组的核蛋白复合物包含外源基因和重新结合的游动孢子可透过病毒的外壳蛋白。在适于将核蛋白复合物传递入细胞的条件下，将植物细胞与动物园接触。该方法可在无菌条件下进行，适用于所有核酸，无论大小如何，可用于将外来基因引入单子叶植物和双子叶植物的细胞中。

84. 发明授权

US5013658A Transposon tagging of genes in transformed plants 失效
标题翻译：转座植物基因转座标记
公开(公告)号：US5013658A
公开(公告)日：1991-05-07
申请号：US193966
申请日：1988-05-13
申请人： Hugo Dooner , Jonathan Jones , Pal Maliga
发明人： Hugo Dooner , Jonathan Jones , Pal Maliga
IPC分类号： C07K14/415 , C12N1/21 , C12N9/12 , C12N15/10 , C12N15/82
CPC分类号： C07K14/415 , C12N15/10 , C12N15/8202 , C12N15/8209 , C12N15/8283 , C12N9/12 , Y10S47/01
摘要： This invention relates to improved methods for transposon tagging as a route to plant gene isolation. The specific improvements comprise efficient means for preselecting plants which have undergone transposition. Through use of this preselection, it is possible to substantially reduce the number of plants which must be inspected to locate individuals having the transposon excised and inserted into a gene of interest. The result is a substantial savings in time, costs and efficiency associated with growing and screening large numbers of plants for tagging and isolation of a desired plant gene.
摘要翻译：本发明涉及转座子标记的改良方法，作为植物基因分离的途径。具体的改进包括用于预选经过转座的植物的有效手段。通过使用这种预选择，可以显着减少必须检查的植物的数量，以定位具有切除并插入目的基因的转座子的个体。结果是与增长和筛选大量用于标记和分离所需植物基因的植物相关联的时间，成本和效率的显着节省。

85. 发明公开

US20240175040A1 CO-REGENERATION RECALCITRANT PLANTS 审中-公开
公开(公告)号：US20240175040A1
公开(公告)日：2024-05-30
申请号：US18487029
申请日：2023-10-13
申请人： Keygene N.V.
发明人： Jeroen STUURMAN , Bipna Rani SHRESTHA , Hugo Ferdinand HOFHUIS
IPC分类号： C12N15/82 , C12N9/22
CPC分类号： C12N15/8202 , C12N9/22 , C12N2310/20
摘要： The invention concerns a method for producing a shoot of a plant comprising germline progenitor cells of a recalcitrant plant. The germline progenitor cells may be modified to comprise a mutation in a sequence of interest. The invention further pertains to plants obtainable by the method of the invention, wherein the plant preferably comprises at least the L2-meristem layer of the recalcitrant plant.

86. 发明公开

US20230407323A1 DELIVERY OF GENOME EDITING REAGENTS VIA CHIMERIC PLANTS 审中-公开
公开(公告)号：US20230407323A1
公开(公告)日：2023-12-21
申请号：US18248010
申请日：2021-10-07
申请人： Calyxt, Inc.
发明人： Nathaniel Butler
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C12N15/8202
摘要： Embodiments of the present disclosure are directed to a method that includes generating a chimeric plant by infecting a cutting of a host plant with Rhizobium rhizogenes carrying an expression construct, and screening new growth from the chimeric plant for gene edits resulting from genomic editing by the expression construct.

87. 发明公开

US20230276765A1 ENDOPHYTE COMPOSITIONS AND METHODS FOR IMPROVEMENT OF PLANT TRAITS 审中-公开
公开(公告)号：US20230276765A1
公开(公告)日：2023-09-07
申请号：US17988113
申请日：2022-11-16
申请人： Indigo Ag, Inc.
发明人： Raymond Riley
IPC分类号： A01H17/00 , C12N15/82 , C05F11/08 , A01N63/30
CPC分类号： A01H17/00 , C12N15/8202 , C12N15/8262 , C05F11/08 , A01N63/30
摘要： This invention relates to methods and materials for modulating the characteristics of a plant, said plant having been heterologously disposed to an endophyte or a plurality of endophytes, or derived from a plant reproductive element heterologously disposed to an endophyte or a plurality of endophytes.

88. 发明授权

US11718856B2 Recombinant micelle and method of in vivo assembly 有权
公开(公告)号：US11718856B2
公开(公告)日：2023-08-08
申请号：US17717000
申请日：2022-04-08
申请人： Mozza Foods, Inc.
发明人： Cory J. Tobin
IPC分类号： C12N15/82 , B01D61/14
CPC分类号： C12N15/8202 , B01D61/145 , B01D61/146 , B01D61/147 , C12N15/8218 , B01D2315/16
摘要： A method of in vivo assembly of a recombinant micelle including: introducing a plasmid into a plant cell, wherein: the plasmid includes a segment of deoxyribonucleic acid (DNA) for encoding a ribonucleic acid (RNA) for a protein in a casein micelle, the segment of DNA is transcribed and translated; forming recombinant casein proteins in the plant cell, wherein: the recombinant casein proteins include a κ-casein and at least one of an αS1-casein, an αS2-casein, a β-casein; and assembling in vivo a recombinant micelle within the plant cell, wherein: an outer layer of the recombinant micelle is enriched with the κ-casein, an inner matrix of the recombinant micelle include at least one of the αS1-casein, the αS2-casein, the β-casein.

89. 发明授权

US10865418B2 Use of multiple transformation enhancer sequences to improve plant transformation efficiency 有权
公开(公告)号：US10865418B2
公开(公告)日：2020-12-15
申请号：US16035556
申请日：2018-07-13
申请人： MONSANTO TECHNOLOGY LLC
发明人： Larry A. Gilbertson , Xudong Ye
IPC分类号： C12N15/84 , C12N15/82 , C12R1/41
CPC分类号： C12N15/8205 , C12R1/41 , C12N15/8202
摘要： The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

90. 发明申请

US20180251774A1 Mutant xylan biosynthetic enzymes capable of dominant suppression of xylan biosynthesis 审中-公开
公开(公告)号：US20180251774A1
公开(公告)日：2018-09-06
申请号：US15847788
申请日：2017-12-19
申请人： The Regents of the University of California
发明人： Andrew Brandon , Henrik Vibe Scheller , Dominique Loque
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8216 , C07K14/415 , C12N9/1077 , C12N15/8202 , C12N15/8246
摘要： The present invention provides for a polypeptide capable of dominant suppression of a first naturally occurring IRX10, wherein the polypeptide comprises an amino acid sequence having at least 70% identity as compared to a second naturally occurring IRX10 wherein the polypeptide comprises one or more of the conserved amino acid indicated in FIG. 2 substituted with a different amino acid residue.

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