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81. 发明授权

US06583271B1 Use of thiol redox proteins for reducing protein intramolecular disulfide bonds, for improving the quality of cereal products, dough and baked goods and for inactivating snake, bee and scorpion toxins 失效
标题翻译：使用硫醇氧化还原蛋白来减少蛋白质分子内二硫键，用于提高谷物，面团和烘焙食品的质量，以及灭活蛇，蜜蜂和蝎毒素
公开(公告)号：US06583271B1
公开(公告)日：2003-06-24
申请号：US09448109
申请日：1999-11-23
申请人： Bob B. Buchanan , Karoly Kobrehel , Boihon C. Yee
发明人： Bob B. Buchanan , Karoly Kobrehel , Boihon C. Yee
IPC分类号： A23J112
CPC分类号： C12P21/02 , A21D2/26 , A21D2/265 , A21D2/266 , A21D8/04 , A23C9/1213 , A23J1/12 , A23J1/14 , A23J3/18 , A23L5/27 , A23L7/109 , A23L7/196 , A23L11/34 , A23L13/48 , A61K38/00 , C07K1/1133 , C07K14/415 , C07K14/46 , C07K14/76 , C07K14/811 , C12N9/0036 , C12N9/20 , C12Y108/01008 , C12Y108/01009 , Y02A50/473 , A61K38/44 , A61K2300/00
摘要： Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods' characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either E. coli or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin. Moreover, the reduced form of the inhibitors showed increased susceptibility to heat and proteolysis by either subtilisin or a protease preparation from germinating wheat seeds. The 2S albumin of castor seed endosperm was reduced by thioredoxin from either wheat germ or E. coli. Thioredoxin was reduced by either NADPH and NADP-thioredoxin reductase or dithiothreitol. Analyses showed that thioredoxin actively reduced the intramolecular disulfides of the 2S large subunit, but was ineffective in reducing the intermolecular disulfides that connect the large to the small subunit. A novel cystine containing protein that inhibits pullulanase was isolated. The protein was reduced by thioredoxin and upon reduction its inhibitory activity was destroyed or greatly reduced.
摘要翻译：提供了减少含有动物和植物蛋白质的方法，以及改善面团和焙烤食品的特性，包括将生面团成分与硫醇氧化还原蛋白混合以形成面团并烘焙面团以形成烤制品的步骤。本发明的方法优选使用减少的硫氧还蛋白与小麦粉，其赋予更强的面团和更高的面包体积。还提供了用硫醇氧化还原（SH）试剂降低蛇，蜂和蝎毒素蛋白，从而使个体灭活蛋白或使蛋白质解毒的方法。包括大豆Kunitz和Bowman-Birk胰蛋白酶抑制剂在内的蛋白酶抑制剂也被来自大肠杆菌或小麦胚芽的NADP /硫氧还蛋白系统（NADPH，硫氧还蛋白和NADP-硫氧还蛋白还原酶）还原。当被硫氧还蛋白减少时，Kunitz和Bowman-Birk大豆胰蛋白酶抑制剂失去了抑制胰蛋白酶的能力。此外，还原形式的抑制剂显示由发芽小麦种子中的枯草杆菌蛋白酶或蛋白酶制剂对热和蛋白水解的敏感性增加。蓖麻种子胚乳的2S白蛋白由小麦胚芽或大肠杆菌的硫氧还蛋白降低。硫氧还蛋白被NADPH和NADP硫氧还蛋白还原酶或二硫苏糖醇还原。分析表明，硫氧还蛋白活性降低了2S大亚基的分子内二硫化物，但在减少连接大亚基与小亚基的分子间二硫化物方面无效。分离出含有蛋白质的新型胱氨酸，其抑制支链淀粉酶。蛋白质被硫氧还蛋白还原，当其还原时，其抑制活性被破坏或大大降低。

82. 发明授权

US5985338A Plant protein for nutritional products and method of making same 失效
公开(公告)号：US5985338A
公开(公告)日：1999-11-16
申请号：US163493
申请日：1998-06-30
申请人： John D. Suh , Karin M. Ostrom , Louis I. Ndife , Paul S. Anloague , James N. Chmura , Andre Daab-Krzykowski , Paul W. Johns , Diane M. Garcia , Terrence B. Mazer , Fu-I Mei
发明人： John D. Suh , Karin M. Ostrom , Louis I. Ndife , Paul S. Anloague , James N. Chmura , Andre Daab-Krzykowski , Paul W. Johns , Diane M. Garcia , Terrence B. Mazer , Fu-I Mei
IPC分类号： A23J3/14 , A23J3/16 , A23L1/015 , A23L1/211 , A23L1/30 , A23K1/02 , A23L1/00
CPC分类号： A23J3/14 , A23J3/16 , A23L11/34 , A23L33/11 , A23L5/273 , Y10S426/801
摘要： The present invention relates generally to a method of producing plant proteins for use in nutritional products that have reduced levels of phytoestrogens, manganese or nucleotides. More specifically, this invention is directed to a method of using ion exchange technology to remove phytoestrogens, manganese or nucleotides from plant proteins. This invention is also directed to the plant protein product resulting from the inventive process and to nutritional products that use the plant protein product as a source of amino nitrogen.

83. 发明授权

US5726034A Aglucone isoflavone enriched vegetable protein extract and protein material, and high genistein and daidzein content materials and process for producing the same 失效
标题翻译：富含肌苷异黄酮的植物蛋白提取物和蛋白质材料，高染料木黄酮和黄豆苷原含量的材料及其制备方法
公开(公告)号：US5726034A
公开(公告)日：1998-03-10
申请号：US709026
申请日：1996-09-06
申请人： Barbara A. Bryan , Maryann C. Allred
发明人： Barbara A. Bryan , Maryann C. Allred
IPC分类号： C12P17/16 , A23J3/14 , A23J3/16 , A23L11/00 , A23L11/30 , A61K31/35 , A61K31/352 , C07D311/36 , C07K14/415 , C12P17/06 , C21P21/06 , A61K35/78 , C12N9/24 , A61K31/337
CPC分类号： C12P17/06 , A23J3/14 , A23J3/16 , A23L11/07 , A23L11/33 , A23L11/34
摘要： An aglucone is isoflavone enriched vegetable protein extract and protein material are provided, as well as a high genistein content material and a high daidzein content material. Isoflavone conjugates in a vegetable material are converted to isoflavone glucosides by treating the vegetable material at a temperature and a pH for a period of time sufficient to effect the conversion. The isoflavone glycosides are converted to glucose isoflavones by enzymatic reaction. The vegetable material is extracted with an aqueous extractant having a pH above about the isoelectric point of protein in the vegetable material to extract protein and the isoflavones either before or after conversion of the isoflavone conjugates to isoflavone glucosides or the conversion of the isoflavone glucosides to aglucone isoflavones. An aglucone isoflavone enriched protein material is produced by precipitating the protein and aglucone isoflavones from the extract. A high genistein content material or a high daidzein content material may be produced from the aglucone isoflavone enriched protein extract or aglucone isoflavone enriched protein material by separating the high genistein or high daidzein content material from the extract or protein material.
摘要翻译：配基是富含异黄酮的植物蛋白提取物和蛋白质材料，以及高染料木黄酮含量的材料和高黄豆苷原含量的材料。将植物材料中的异黄酮共轭物通过在温度和pH下处理植物材料一段足以实现转化的时间转化为异黄酮糖苷。异黄酮糖苷通过酶反应转化成葡萄糖异黄酮。植物材料用pH高于植物原料蛋白质等电点的水提取剂提取，以在将异黄酮共轭物转化为异黄酮糖苷之前或之后提取蛋白质和异黄酮，或将异黄酮糖苷转化成配基异黄酮通过从提取物中沉淀蛋白质和配基异黄酮产生富含配基异黄酮的蛋白质材料。通过从提取物或蛋白质材料中分离高染料木黄酮或高黄豆苷原含量的材料，可以从富含配基异黄酮的蛋白质提取物或富含配基异黄酮的蛋白质材料中产生高染料木黄酮含量的材料或高黄豆苷原含量的材料。

84. 发明授权

US5306810A Separation of manganese from rice protein using alumina 失效
标题翻译：使用氧化铝从锰蛋白分离锰
公开(公告)号：US5306810A
公开(公告)日：1994-04-26
申请号：US72165
申请日：1993-06-07
申请人： Terrence B. Mazer , Christy A. Nardelli , Arthur J. C. L. Hogarth , Andre Daab-Krzykowski
发明人： Terrence B. Mazer , Christy A. Nardelli , Arthur J. C. L. Hogarth , Andre Daab-Krzykowski
IPC分类号： A23J1/14 , A23L1/211 , A23L1/308 , A23J1/12 , A23J3/14
CPC分类号： A23J1/14 , A23L11/34 , A23L33/21
摘要： A method for separating phytate and manganese from protein and dietary fiber involves treatment of an aqueous slurry of phytatecontaining material at a low pH with insoluble alumina. In a batch treatment process the pH of the solution is increased, leaving phytate units attached to the alumina while freeing the protein and dietary fiber. In a column treatment process, the column containing alumina is rinsed, after the low pH treatment, with dilute acid and water to recover the protein and/or dietary fiber. This method may be employed either during the manufacture of protein and fiber isolates from flour or flakes, or for removing phytate from commercially available protein and fiber commodities. The spent alumina may be readily regenerated and reused. A method of separating manganese from rice protein using this same technology is also disclosed.
摘要翻译：从蛋白质和膳食纤维中分离植酸盐和锰的方法包括在低pH下用不溶性氧化铝处理含植酸盐的材料的含水浆液。在分批处理过程中，溶液的pH值增加，留下植酸盐单位连接到氧化铝上，同时释放蛋白质和膳食纤维。在柱处理过程中，在低pH处理后，用稀酸和水冲洗含有氧化铝的柱，以回收蛋白质和/或膳食纤维。该方法可以在从面粉或薄片制造蛋白质和纤维分离物期间使用，或用于从市售的蛋白质和纤维商品中除去植酸盐。废氧化铝可以容易地再生和重复使用。还公开了使用这种相同技术从米蛋白中分离锰的方法。

85. 发明授权

US5248765A Separation of phytate from plant protein and dietary fiber using alumina 失效
标题翻译：使用氧化铝从植物蛋白和膳食纤维中分离植物
公开(公告)号：US5248765A
公开(公告)日：1993-09-28
申请号：US811102
申请日：1991-12-20
申请人： Terrence B. Mazer , Christy A. Nardelli , Arthur J. C. L. Hogarth , Andre Daab-Krzykowski
发明人： Terrence B. Mazer , Christy A. Nardelli , Arthur J. C. L. Hogarth , Andre Daab-Krzykowski
IPC分类号： A23J1/14 , A23L1/211 , A23L1/308
CPC分类号： A23J1/14 , A23L11/34 , A23L33/21
摘要： A method for separating phytate and manganese from protein and dietary fiber involves treatment of an aqueous slurry of phytate-containing material at a low pH with insoluble alumina. In a batch treatment process the pH of the solution is increased, leaving phytate units attached to the alumina while freeing the protein and dietary fiber. In a column treatment process, the column containing alumina is rinsed, after the low pH treatment, with dilute acid and water to recover the protein and/or dietary fiber. This method may be employed either during the manufacture of protein and fiber isolates from flour or flakes, or for removing phytate from commercially available protein and fiber commodities. The spent alumina may be readily regenerated and reused. A method of separating manganese from rice protein using this same technology is also disclosed.
摘要翻译：从蛋白质和膳食纤维中分离植酸盐和锰的方法包括在低pH下用不溶性氧化铝处理含植酸盐的材料的含水浆液。在分批处理过程中，溶液的pH值增加，留下植酸盐单位连接到氧化铝上，同时释放蛋白质和膳食纤维。在柱处理过程中，在低pH处理后，用稀酸和水冲洗含有氧化铝的柱，以回收蛋白质和/或膳食纤维。该方法可以在从面粉或薄片制造蛋白质和纤维分离物期间使用，或用于从市售的蛋白质和纤维商品中除去植酸盐。废氧化铝可以容易地再生和重复使用。还公开了使用这种相同技术从米蛋白中分离锰的方法。

86. 发明授权

US3043856A Removal of gossypol from cotton seed products 失效
标题翻译：从棉花种子产品中去除棉酚
公开(公告)号：US3043856A
公开(公告)日：1962-07-10
申请号：US77954758
申请日：1958-12-11
申请人： COUNCIL SCIENT IND RES
发明人： RAJENDRA SESHADRI TIRUVENKATA , KAILASH CHANDER
IPC分类号： A23L11/30 , C11B3/06
CPC分类号： C11B3/06 , A23L11/34

87. 发明授权

US2641542A Detoxifying tung meal 失效
标题翻译：排毒汤粉
公开(公告)号：US2641542A
公开(公告)日：1953-06-09
申请号：US17479750
申请日：1950-07-19
申请人： M DARSEY VAN
发明人： ULREY DAVID G
IPC分类号： A23L11/30
CPC分类号： A23L11/34

88. 发明授权

US2260640A Method of treating seeds rich in protein 失效
标题翻译：处理富含蛋白质的种子的方法
公开(公告)号：US2260640A
公开(公告)日：1941-10-28
申请号：US30119639
申请日：1939-10-25
申请人： WEST VIRGINIA PULP & PAPER CO
发明人： RAWLING FRANCIS G , WELTON WRIGHT M
IPC分类号： A23L11/30
CPC分类号： A23L11/34

89. 发明授权

US2147097A Developing palatability of soya 失效
标题翻译：发展大豆的适口性
公开(公告)号：US2147097A
公开(公告)日：1939-02-14
申请号：US15619037
申请日：1937-07-28
申请人： SOYA CORP
发明人： HORVATH ARTEMY A
IPC分类号： A23L11/30
CPC分类号： A23L11/34

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