专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

81. 发明授权

US5973136A Inverted chimeric oligonucleotides 失效
标题翻译：倒置嵌合寡核苷酸
公开(公告)号：US5973136A
公开(公告)日：1999-10-26
申请号：US886670
申请日：1997-07-01
申请人： Sudhir Agrawal
发明人： Sudhir Agrawal
IPC分类号： C12N15/09 , A61K31/70 , A61K31/7088 , A61K31/712 , A61K38/00 , A61K48/00 , C07H21/00 , C12N15/113 , C07H21/04
CPC分类号： C12N15/1137 , C07H21/00 , A61K38/00 , C12N2310/315 , C12N2310/321 , C12N2310/322 , C12N2310/341 , C12N2310/345 , C12N2310/346
摘要： The invention relates to modified oligonucleotides that are useful for studies of gene expression and for the antisense therapeutic approach. The invention provides inverted hybrid oligonucleotides and inverted chimeric oligonucleotides, both of which produce reduced side effects, relative to traditional phosphorothioate, hybrid or chimeric oligonucleotides.
摘要翻译：本发明涉及可用于研究基因表达和反义治疗方法的修饰的寡核苷酸。本发明提供了相对于传统的硫代磷酸酯，杂交或嵌合寡核苷酸的逆转杂交寡核苷酸和反向嵌合寡核苷酸，两者都产生副作用降低。

82. 发明授权

US5962674A Synthesis of oligonucleotides containing alkylphosphonate internucleoside linkages 失效
标题翻译：含有烷基膦酸酯核苷间键的寡核苷酸的合成
公开(公告)号：US5962674A
公开(公告)日：1999-10-05
申请号：US606915
申请日：1996-02-26
申请人： Radhakrishnan P. Iyer , Theresa Devlin , Ivan Habus , Dong Yu , Sudhir Agrawal
发明人： Radhakrishnan P. Iyer , Theresa Devlin , Ivan Habus , Dong Yu , Sudhir Agrawal
IPC分类号： C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00
CPC分类号： C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , Y02P20/55
摘要： The invention provides new reagents and an improved process for synthesizing oligonucleotides that contain methylphosphonate internucleoside linkages. The reagents and process utilize a nucleoside base protecting group that is stable under oligonucleotide synthesis conditions, but which can be removed under more mild conditions than existing protecting groups.
摘要翻译：本发明提供新的试剂和用于合成含有甲基膦酸核苷间键的寡核苷酸的改进方法。试剂和方法利用在寡核苷酸合成条件下稳定的核苷碱基保护基，但可以在比现有保护基更温和的条件下除去。

83. 发明授权

US5955599A Process for making oligonucleotides containing o- and s- methylphosphotriester internucleoside linkages 失效
标题翻译：制备含有O-和S-甲基磷酸核苷间核苷键的寡核苷酸的方法
公开(公告)号：US5955599A
公开(公告)日：1999-09-21
申请号：US570390
申请日：1995-12-11
申请人： Radhakrishnan P. Iyer , Theresa Devlin , Ivan Habus , Dong Yu , Sudhir Agrawal
发明人： Radhakrishnan P. Iyer , Theresa Devlin , Ivan Habus , Dong Yu , Sudhir Agrawal
IPC分类号： C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H21/04
CPC分类号： C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00
摘要： The invention provides oligonucleotides containing methyl phosphotriester linkages and processes for making and methods for using such oligonucleotides.
摘要翻译：本发明提供了含有甲基磷酸三酯键的寡核苷酸及其制备方法和使用这些寡核苷酸的方法。

84. 发明授权

US5856462A Oligonucleotides having modified CpG dinucleosides 失效
标题翻译：具有修饰的CpG二核苷的寡核苷酸
公开(公告)号：US5856462A
公开(公告)日：1999-01-05
申请号：US711568
申请日：1996-09-10
申请人： Sudhir Agrawal
发明人： Sudhir Agrawal
IPC分类号： C12N15/09 , A61K31/7115 , A61K31/712 , A61K31/7125 , A61K38/00 , C07H21/00 , C12N15/113 , C07H21/04
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/31 , C12N2310/311 , C12N2310/312 , C12N2310/314 , C12N2310/315 , C12N2310/321 , C12N2310/3341
摘要： The invention relates to modified oligonucleotides that are useful for studies of gene expression and for the antisense therapeutic approach. The invention provides modified oligonucleotides that inhibit gene expression and that produce fewer side effects than conventional phosphorothioate oligonucleotides. In particular, the invention provides modified CpG-containing oligonucleotides that result in reduced splenomegaly and platelet depletion when administered to a mammal, relative to conventional CpG-containing phosphorothioate oligonucleotides. The invention further provides methods for using such oligonucleotides to modulate gene expression in vivo, including such use for therapeutic treatment of diseases caused by aberrant gene expression.
摘要翻译：本发明涉及可用于研究基因表达和反义治疗方法的修饰的寡核苷酸。本发明提供了修饰的寡核苷酸，其抑制基因表达并产生比常规硫代磷酸酯寡核苷酸更少的副作用。特别地，本发明提供了相对于常规的含CpG的硫代磷酸酯寡核苷酸，当给予哺乳动物时，本发明提供了修饰的含CpG的寡核苷酸，其导致减少的脾肿大和血小板减少。本发明还提供了使用这样的寡核苷酸来调节体内基因表达的方法，包括用于治疗由异常基因表达引起的疾病的用途。

85. 发明授权

US5750674A Methods and compounds for the stereoselective enrichment of oligonucleotide diastereomers and oligonucleotides thereby produced 失效
标题翻译：由此制备寡核苷酸非对映异构体和寡核苷酸的立体选择性富集的方法和化合物
公开(公告)号：US5750674A
公开(公告)日：1998-05-12
申请号：US448131
申请日：1995-05-23
申请人： Radhakrishnan P. Iyer , Dong Yu , Sudhir Agrawal , Weitian Tan
发明人： Radhakrishnan P. Iyer , Dong Yu , Sudhir Agrawal , Weitian Tan
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00
CPC分类号： C07H21/00 , C07H19/10 , C07H19/20
摘要： The present invention provides new mononucleotide synthons useful in the synthesis of oligonucleotides having from one to all P-chiral centers that are predominantly and independently in the R or S configuration. The invention also provides methods of synthesizing these synthons, methods of synthesizing oligonucleotides having from one to all P-chiral centers predominantly and independently in the R or S configuration, and such oligonucleotides. Oligonucleotides synthesized with the novel synthons are useful for modulating nucleic acid expression, both in vitro and in vivo, as well as in traditional hybridization assays.
摘要翻译：本发明提供了可用于合成寡核苷酸的新的单核苷酸合成子，所述寡核苷酸具有主要且独立于R或S构型的一个至所有P-手性中心。本发明还提供了合成这些合成子的方法，合成寡核苷酸的方法，所述寡核苷酸主要以R或S构型主要且独立地具有一个至所有的P-手性中心，以及这些寡核苷酸。用新型合成子合成的寡核苷酸可用于在体外和体内以及传统杂交测定中调节核酸表达。

86. 发明授权

US5739308A Integrated oligonucleotides 失效
标题翻译：综合寡核苷酸
公开(公告)号：US5739308A
公开(公告)日：1998-04-14
申请号：US418123
申请日：1995-04-06
申请人： Ekambar R. Kandimalla , Sudhir Agrawal
发明人： Ekambar R. Kandimalla , Sudhir Agrawal
IPC分类号： C12N15/09 , A61K31/70 , A61P9/00 , A61P31/12 , A61P35/00 , A61P37/00 , A61P43/00 , C07H21/00 , C07H21/04 , C12N15/113
CPC分类号： C12N15/113 , C07H21/00 , C12N15/1132 , C12N2310/152 , C12N2310/321 , C12N2310/3527 , C12N2310/53
摘要： The invention provides novel oligonucleotides that form a duplex with a target nucleic acid and then form a triplex with the duplex formed between the oligonucleotide and the target nucleic acid.
摘要翻译：本发明提供与靶核酸形成双链体，然后与寡核苷酸和靶核酸之间形成的双链体形成三链体的新型寡核苷酸。

87. 发明授权

US5652103A Method for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages 失效
标题翻译：用于测序含有非磷酸二酯核苷酸间键的合成寡核苷酸的方法
公开(公告)号：US5652103A
公开(公告)日：1997-07-29
申请号：US412913
申请日：1995-03-29
申请人： Sudhir Agrawal , Jin-Yan Tang
发明人： Sudhir Agrawal , Jin-Yan Tang
IPC分类号： C12N15/09 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6855 , C12Q1/6869 , Y10T436/143333
摘要： The invention relates to synthetic oligonucleotides and more particularly to the determination of nucleotide sequences of synthetic oligonucleotides having non-phosphodiester internucleotide linkages. The invention provides a method for sequencing such modified synthetic oligonucleotides.
摘要翻译：本发明涉及合成的寡核苷酸，更具体地涉及确定具有非磷酸二酯核苷酸间键的合成寡核苷酸的核苷酸序列。本发明提供了对这种修饰的合成寡核苷酸进行测序的方法。

88. 发明授权

US5510475A Oligonucleotide multiple reporter precursors 失效
标题翻译：寡核苷酸多重记录前体
公开(公告)号：US5510475A
公开(公告)日：1996-04-23
申请号：US320835
申请日：1994-10-07
申请人： Sudhir Agrawal , Jin-Yan Tang
发明人： Sudhir Agrawal , Jin-Yan Tang
IPC分类号： C07H21/04 , C07F9/141 , C07H21/00 , C12N15/09 , C12Q1/68
CPC分类号： C07H21/00 , C07F9/1411
摘要： Oligonucleotides containing multiple free amino groups attached directly to the phosphorus atoms of the phosphodiester linkages having the structure shown below. ##STR1## wherein D=a ribonucleoside or deoxyribonucleotide;p=a 5' to 3'-phosphodiester or phosphoramidate linkage;n=1 to 20;a=1 to 20;b=1 to 20;c=1=20;z=0 to 20;t=1 to 100; andwhereinfor each repeating unit, "a" represents the same number or a different number than that number represented by "a" in every other repeating unit; and for each repeating unit "b" represents the same number or a different number than represented by "b" in every other repeating unit.Multiple reporter molecules can be incorporated into the oligonucleotide via the free amino groups allowing for increased probe sensitivity.
摘要翻译：含有多个游离氨基的寡核苷酸，其直接连接到具有下面所示结构的磷酸二酯键的磷原子上。其中D =核糖核苷或脱氧核糖核苷酸; p = 5'至3'-磷酸二酯或氨基磷酸酯键; n = 1〜20; a = 1〜20; b = 1〜20; c = 1 = 20; z = 0〜20; t = 1〜100; 并且其中对于每个重复单元，“a”表示与每隔一个重复单元中由“a”表示的数字相同的数字或不同的数字; 并且对于每个重复单元“b”表示与每隔一个重复单元中的“b”相同的数字或不同的数字。多个报道分子可以通过游离氨基引入寡核苷酸，从而增加探针灵敏度。

89. 发明授权

US5366878A Method of site-specific alteration of RNA and production of encoded polypeptides 失效
标题翻译： RNA的位点特异性改变方法和编码多肽的产生
公开(公告)号：US5366878A
公开(公告)日：1994-11-22
申请号：US36503
申请日：1993-03-24
申请人： Thoru Pederson , Sudhir Agrawal , Sandra Mayrand , Paul C. Zamecnik
发明人： Thoru Pederson , Sudhir Agrawal , Sandra Mayrand , Paul C. Zamecnik
IPC分类号： C12N15/09 , C12N15/10 , C12N15/113 , C12N15/82 , C12N15/11 , C07H21/00 , C12P19/34
CPC分类号： C12N15/113 , C12N15/102 , C12N15/8233 , C12N15/8235 , C12N2310/3125 , C12N2310/314 , C12N2310/315
摘要： A method of site-directed alteration (removal or removal followed by replacement) of selected nucleotides in an RNA molecule, as well as to mixed phosphate backbone oligonucleotides useful in the method. It further relates to a method of producing polypeptides or proteins encoded by the RNA molecule altered by the present method. Through use of the present method, site-directed cleavage of an RNA molecule is effected, followed by excision of the selected or target segment of the RNA molecule.
摘要翻译：在RNA分子中选择的核苷酸的定点改变（去除或去除，随后替换）的方法以及在该方法中有用的混合的磷酸酯骨架寡核苷酸。本发明还涉及生产由本发明方法改变的RNA分子编码的多肽或蛋白质的方法。通过使用本方法，实现RNA分子的定点切割，然后切除RNA分子的所选择的或靶区段。

90. 发明授权

US5149797A Method of site-specific alteration of RNA and production of encoded polypeptides 失效
标题翻译： RNA的特异性修饰方法和编码的多聚体的生产方法
公开(公告)号：US5149797A
公开(公告)日：1992-09-22
申请号：US480269
申请日：1990-02-15
申请人： Thoru Pederson , Sudhir Agrawal , Sandra Mayrand , Paul C. Zamecnik
发明人： Thoru Pederson , Sudhir Agrawal , Sandra Mayrand , Paul C. Zamecnik
IPC分类号： C12N15/09 , C12N15/10 , C12N15/113 , C12N15/82
CPC分类号： C12N15/113 , C12N15/102 , C12N15/8233 , C12N15/8235 , C12N2310/3125 , C12N2310/314 , C12N2310/315
摘要： A method of site-directed alteration (removal or removal followed by replacement) of selected nucleotides in an RNA molecule, as well as to mixed phosphate backbone oligonucleotides useful in the method. It further relates to a method of producing polypeptides or proteins encoded by the RNA molecule altered by the present method. Through use of the present method, site-directed cleavage of an RNA molecule is effected, followed by excision of the selected or target segment of the RNA molecule.
摘要翻译：在RNA分子中选择的核苷酸的定点改变（去除或去除，随后替换）的方法以及在该方法中有用的混合的磷酸酯骨架寡核苷酸。本发明还涉及生产由本发明方法改变的RNA分子编码的多肽或蛋白质的方法。通过使用本方法，实现RNA分子的定点切割，然后切除RNA分子的所选择的或靶区段。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式