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81. 发明授权

US07906282B2 Synthetic nucleic acid molecule compositions and methods of preparation 有权
标题翻译：合成核酸分子组合物和制备方法
公开(公告)号：US07906282B2
公开(公告)日：2011-03-15
申请号：US11786785
申请日：2007-04-12
申请人： Keith V. Wood , Monika G. Gruber , Yao Zhuang , Aileen Paguio
发明人： Keith V. Wood , Monika G. Gruber , Yao Zhuang , Aileen Paguio
IPC分类号： C12N15/53 , C12N5/10 , C12N15/63
CPC分类号： C12N9/0069 , C12N15/67
摘要： A method to prepare synthetic nucleic acid molecules having reduced inappropriate or unintended transcriptional characteristics when expressed in a particular host cell.
摘要翻译：制备在特定宿主细胞中表达时具有降低的不适当或非预期转录特征的合成核酸分子的方法。

82. 发明申请

US20100281552A1 SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT 有权
标题翻译：具有增强光输出功能的合成眼科植入物
公开(公告)号：US20100281552A1
公开(公告)日：2010-11-04
申请号：US12773002
申请日：2010-05-03
申请人： Lance P. Encell , Keith V. Wood , Monika G. Wood , Mary Hall , Paul Otto , Gediminas Vidugiris , Kristopher Zimmerman
发明人： Lance P. Encell , Keith V. Wood , Monika G. Wood , Mary Hall , Paul Otto , Gediminas Vidugiris , Kristopher Zimmerman
IPC分类号： A01K67/027 , C07H21/00 , C12N15/63 , C12N5/10 , C12N9/02
CPC分类号： C12Q1/66 , C12N9/0004
摘要： A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
摘要翻译：编码经修饰的萤光素酶多肽的多核苷酸。修饰的荧光素酶多肽与野生型Oplophorus萤光素酶具有至少60％的氨基酸序列同一性，并且在对应于SEQ ID NO：1的野生型Oplophorus荧光素酶中的氨基酸的位置包括至少一个氨基酸取代。修饰的荧光素酶多肽相对于野生型Oplophorus萤光素酶具有增强的发光，增强的信号稳定性和增强的蛋白质稳定性中的至少一种。

83. 发明申请

US20100249427A1 BIOLUMINESCENT PROTEASE ASSAY 有权
标题翻译：生物发光蛋白质测定
公开(公告)号：US20100249427A1
公开(公告)日：2010-09-30
申请号：US12706078
申请日：2010-02-16
申请人： Martha O'Brien , Keith V. Wood , Dieter Klaubert , William Daily
发明人： Martha O'Brien , Keith V. Wood , Dieter Klaubert , William Daily
IPC分类号： C07D277/64
CPC分类号： C07D277/66 , C12Q1/37 , C12Q1/66
摘要： A sensitive bioluminescent assay to detect proteases including caspases, trypsin and tryptase is provided.
摘要翻译：提供了敏感的生物发光测定法来检测蛋白酶，包括胱天蛋白酶，胰蛋白酶和类胰蛋白酶。

84. 发明授权

US07666987B2 Bioluminescent caspase assay compounds 有权
标题翻译：生物发光半胱天冬酶测定化合物
公开(公告)号：US07666987B2
公开(公告)日：2010-02-23
申请号：US11347054
申请日：2006-02-03
申请人： Martha O'Brien , Keith V. Wood , Dieter Klaubert , William Daily
发明人： Martha O'Brien , Keith V. Wood , Dieter Klaubert , William Daily
IPC分类号： C07K1/00
CPC分类号： C07D277/66 , C12Q1/37 , C12Q1/66
摘要： A sensitive bioluminescent assay to detect proteases including caspases is provided which employs an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a substrate for a caspase or an aminoluciferin or a carboxy-terminal protected derivative thereof covalently linked via a peptide bond to a peptide substrate comprising aspartate that is specifically cleaved by a protease specific for the substrate.
摘要翻译：提供了用于检测包括胱天蛋白酶的蛋白酶的敏感生物发光测定法，其使用通过肽键共价连接的氨基荧光素或其羧基端保护的衍生物与底物进行共价连接的胱天蛋白酶或氨基荧光素或羧基端保护的衍生物肽结合到包含由对底物特异的蛋白酶特异性切割的天冬氨酸的肽底物。

85. 发明申请

US20090253131A1 HYBRID FUSION REPORTER AND USES THEREOF 审中-公开
标题翻译：混合熔融报告器及其用途
公开(公告)号：US20090253131A1
公开(公告)日：2009-10-08
申请号：US12265558
申请日：2008-11-05
申请人： Susan Wigdal , Keith V. Wood
发明人： Susan Wigdal , Keith V. Wood
IPC分类号： C12Q1/68 , G01N33/573 , C12N9/04 , C12N5/00 , C12N15/63
CPC分类号： C12Q1/66 , C12Q1/6897 , C12Q2565/201
摘要： The invention provides vectors encoding hybrid fusion proteins and vector sets encoding different hybrid fusion proteins useful, for instance, in protein complementation assays.
摘要翻译：本发明提供了编码杂交融合蛋白的载体和编码不同杂合融合蛋白的载体集，其可用于例如蛋白质互补测定。

86. 发明授权

US07148030B2 Bioluminescent protease assay 有权
公开(公告)号：US07148030B2
公开(公告)日：2006-12-12
申请号：US10356665
申请日：2003-01-31
申请人： Martha O'Brien , Keith V. Wood , Dieter Klaubert , William Daily
发明人： Martha O'Brien , Keith V. Wood , Dieter Klaubert , William Daily
IPC分类号： C12Q1/66
CPC分类号： C07D277/66 , C12Q1/37 , C12Q1/66
摘要： A sensitive bioluminescent assay to detect proteases including caspases, trypsin and tryptase is provided.

87. 发明授权

US5670356A Modified luciferase 失效
标题翻译：改良荧光素酶
公开(公告)号：US5670356A
公开(公告)日：1997-09-23
申请号：US354240
申请日：1994-12-12
申请人： Bruce A. Sherf , Keith V. Wood
发明人： Bruce A. Sherf , Keith V. Wood
IPC分类号： C12N9/02 , C12N15/53 , C07H21/04 , C12N5/10
CPC分类号： C12N9/0069
摘要： A modified form of beetle luciferase, which has been engineered for improved genetic reporting, is disclosed. The modified form contains one or more new features. Chief among these is removal of the peroxisomal translocation sequence to yield a cytoplasmic form of the enzyme. Other changes include removal of potentially interfering restriction sites and genetic regulatory sites from the gene, improvement of the codon usage for mammalian cells. The modified luciferase reporter enzyme is also devoid of potential N-glycosylation targets to minimize post-translational modification and remains in the cytoplasm of host cells to optimize substrate availability.
摘要翻译：披露了改进的甲壳质萤光素酶的形式，其已被设计用于改进遗传报告。修改后的表单包含一个或多个新功能。其中主要是去除过氧化物酶体易位序列以产生酶的细胞质形式。其他变化包括从基因中去除潜在的干扰性限制性位点和遗传调控位点，改善哺乳动物细胞的密码子使用。修饰的萤光素酶报道酶也没有潜在的N-糖基化靶标以最小化翻译后修饰并保留在宿主细胞的细胞质中以优化底物可利用性。

88. 发明授权

US08822170B2 Thermostable luciferases and methods of production 有权
标题翻译：热稳定性荧光素酶和生产方法
公开(公告)号：US08822170B2
公开(公告)日：2014-09-02
申请号：US13213457
申请日：2011-08-19
申请人： Keith V. Wood , Monika G. Wood , Mary P. Hall
发明人： Keith V. Wood , Monika G. Wood , Mary P. Hall
IPC分类号： C12N9/02 , C12N11/00 , C12Q1/66
CPC分类号： C12N9/0069 , C07K2319/00 , C12N15/10 , Y10S977/898
摘要： Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
摘要翻译：公开了具有大大增加的热稳定性的荧光素酶，例如在50℃下2小时的至少半衰期，编码新的荧光素酶的cDNA和转化以表达荧光素酶的宿主。产生萤光素酶的方法包括递归诱变。荧光素酶用于常规方法，一些采用试剂盒。

89. 发明授权

US08779221B2 Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule 有权
标题翻译：通过与固定化的脱卤酶底物结合并直接或间接连接到蛋白质或分子的突变体脱卤酶固定蛋白质或分子的方法
公开(公告)号：US08779221B2
公开(公告)日：2014-07-15
申请号：US13477593
申请日：2012-05-22
申请人： Aldis Darzins , Lance Encell , Tonny Johnson , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
发明人： Aldis Darzins , Lance Encell , Tonny Johnson , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
IPC分类号： C07C19/00 , C07C21/02 , C40B40/10 , G01N33/58
CPC分类号： C12N9/14 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07K2299/00 , C12N9/0069 , C12Q1/26 , C12Q1/34 , C12Q1/66 , C12Y113/12005 , C12Y308/01005
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
摘要翻译：提供任选融合到感兴趣的蛋白质的突变体水解酶。突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代野生型水解酶。还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

90. 发明申请

US20130337539A1 COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR 有权
公开(公告)号：US20130337539A1
公开(公告)日：2013-12-19
申请号：US13895119
申请日：2013-05-15
申请人： Aldis Darzins , Lance Encell , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
发明人： Aldis Darzins , Lance Encell , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
IPC分类号： C12Q1/34 , C12N9/14
CPC分类号： G01N33/581 , C07K7/06 , C07K16/00 , C07K2317/622 , C07K2319/43 , C07K2319/61 , C09B1/00 , C09B11/24 , C09B23/04 , C09B23/06 , C09B23/105 , C12N9/14 , C12Q1/34 , C12Y308/01005 , G01N33/573 , G01N2333/914 , Y10S530/825
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

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