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81. 发明授权

US06613883B1 Screening assays for compounds that cause apoptosis and related compounds 失效
标题翻译：导致凋亡和相关化合物的化合物的筛选测定
公开(公告)号：US06613883B1
公开(公告)日：2003-09-02
申请号：US09248776
申请日：1999-02-12
申请人： Curtis C. Harris , Xin Wei Wang , Jan H. J. Hoeijmakers
发明人： Curtis C. Harris , Xin Wei Wang , Jan H. J. Hoeijmakers
IPC分类号： C07K700
CPC分类号： A61K38/1709 , C07K14/4746 , C07K2319/00
摘要： This invention relates to methods of screening for compounds capable of inducing apoptosis in certain tumor cells. The invention also relates to compounds identified by such methods. In addition, the invention relates to methods for the in vitro diagnosis of Xeroderma pigmentosum and compounds useful in these methods.
摘要翻译：本发明涉及筛选能够诱导某些肿瘤细胞凋亡的化合物的方法。本发明还涉及通过这些方法鉴定的化合物。此外，本发明涉及用于这些方法中有用的皮肤色素调色剂和化合物的体外诊断方法。

82. 发明授权

US5432060A Immortalized human bronchial epithelial cell line 失效
标题翻译：永生化人支气管上皮细胞系
公开(公告)号：US5432060A
公开(公告)日：1995-07-11
申请号：US840625
申请日：1992-02-21
申请人： James C. Willey , Curtis C. Harris
发明人： James C. Willey , Curtis C. Harris
IPC分类号： C12N5/071 , C12Q1/02 , C12N5/08
CPC分类号： C12N5/0688 , C12N2510/04
摘要： Human bronchial epithelial cell lines permanently transformed by human papilloma viruses have been obtained. These cell lines are useful for the study of growth and differentiation in bronchial carcinoma and the identification of chemical and biological agents that may be useful in the therapy of human lung cancer.
摘要翻译：已经获得了由人乳头状瘤病毒永久转化的人支气管上皮细胞系。这些细胞系可用于研究支气管癌的生长和分化以及可用于治疗人肺癌的化学和生物制剂的鉴定。

83. 发明授权

US4608339A Protoplast fusion method for high-frequency DNA transfection in human cells 失效
标题翻译：人体细胞高频DNA转染的原生质体融合方法
公开(公告)号：US4608339A
公开(公告)日：1986-08-26
申请号：US545257
申请日：1983-10-25
申请人： George H. Yoakum , Curtis C. Harris , Brent E. Korba , John F. Lechner
发明人： George H. Yoakum , Curtis C. Harris , Brent E. Korba , John F. Lechner
IPC分类号： C07K14/02 , C12N5/16 , C12N15/87 , C12N15/00 , C12N5/00 , C12P21/02
CPC分类号： C07K14/005 , C12N15/87 , C12N5/16 , C12N2510/00 , C12N2730/10122
摘要： A modified protoplast fusion method and cell line is disclosed that stably transfects human cells with pSV2-derived plasmids at frequencies greater than 10.sup.-3. This procedure makes it possible to test the biological effect of individual genes (i.e., oncogenes and other cellular genes, and viral genes). To demonstrate the utility of this invention, a pSV2gpt.sup.+ plasmid constructed to carry a subgenomic fragment of hepatitis B virus (HBV) that contained the core antigen gene (HBc gene) is transfected into human cells. Human cell lines are stably transfected with the HBC.sup.+ gene by selecting recipient cells for expression of guanine phosphoribosyl transferase expression; other selective markers, i.e., neomycin resistance, can also be used. Conditions for enhancing the expression of the transfected gene(s) have also been developed. For example, with this gpt.sup.+ /HBc.sup.+ cell line it is shown that growth in serum-free medium or treatment with 5'-azacytidine stimulates the production of the HBV core antigen.
摘要翻译：公开了修饰的原生质体融合方法和细胞系，其以大于10-3的频率以pSV2衍生的质粒稳定转染人细胞。该方法可以测试各基因（即癌基因和其他细胞基因以及病毒基因）的生物学效应。为了证明本发明的实用性，将构建用于携带含有核心抗原基因（HBc基因）的乙型肝炎病毒（HBV）亚基因组片段的pSV2gpt +质粒转染入人细胞。通过选择用于表达鸟嘌呤磷酸核糖转移酶表达的受体细胞，用HBC +基因稳定转染人细胞系; 也可以使用其他选择标记，即新霉素抗性。还开发了用于增强转染基因表达的条件。例如，使用这种gpt + / HBc +细胞系，显示无血清培养基的生长或用5'-氮杂胞苷碱处理刺激HBV核心抗原的产生。

84. 发明授权

US4289748A Ultrasensitive enzymatic radioimmunoassay method 失效
标题翻译：超敏酶放免法测定法
公开(公告)号：US4289748A
公开(公告)日：1981-09-15
申请号：US44260
申请日：1979-05-31
申请人： Curtis C. Harris , Robert Yolken , Ih-Chang Hsu
发明人： Curtis C. Harris , Robert Yolken , Ih-Chang Hsu
IPC分类号： G01N33/00 , G01N30/96 , G01N33/531 , G01N33/534 , G01N33/536 , G01N33/543 , G01N33/48 , G01N33/54 , G01T1/00
CPC分类号： G01N33/534 , G01N33/54306 , Y10S436/811
摘要： A method of quantitatively measuring antigen or antibody concentration by selectively coupling an antigen to a solid substrate, coupling an enzyme-conjugated antibody to the antigen and reacting a radioindicator with the enzyme-conjugate producing a radiolabelled product. The product is scintillation counted to yield the measurement. Direct or indirect coupling of the antigen to the substrate can be used. Direct or indirect coupling of the enzyme-conjugate can be used. The preferred radioindicator is adenosine-5'-monophosphate used with alkaline phosphatase. Others are presented.
摘要翻译：通过选择性地将抗原偶联到固体底物上，将酶偶联的抗体与抗原偶联并使放射性指示剂与产生放射性标记的产物的酶 - 缀合物反应来定量测量抗原或抗体浓度的方法。产物闪烁计数以产生测量。可以使用抗原与基质的直接或间接偶联。可以使用酶 - 缀合物的直接或间接偶联。优选的放射指示剂是与碱性磷酸酶一起使用的腺苷-5'-一磷酸。其他的被呈现。

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