会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 76. 发明授权
    • Cold-active protease CP70
    • 冷活性蛋白酶CP70
    • US06200793B1
    • 2001-03-13
    • US09117245
    • 1998-07-27
    • A. K. M. Quamrul HasanEiichi Tamiya
    • A. K. M. Quamrul HasanEiichi Tamiya
    • C12N952
    • A23J3/341C12N9/52Y10S435/85
    • A psychrophilic protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, hemoglobin and albumin to specifically decompose them, and the substrate specificity decreases in the order of casein, gelatin, hemoglobin and albumin; (b) optimal pH: 8.0; (c) pH stability: the protease is stable in the range of pH 6.5 to pH 10.0 at 30° C. for 1 hour; (d) optimal temperature: about 40° C.; (e) temperature stability: at pH 7 for 1 hour, the protease is active at a temperature up to 30° C., but it is inactivated at 40° C. as much as about 40% and completely inactivated at 50° C. in about 10 minutes; (f) enzyme activity: the protease has 50% or more of its maximum activity at about 20° C.; (g) the active center of the enzyme is serine; and (h) the molecular weight of the protease is about 70 kDa as measured by SDS-PAGE.
    • 这里公开了一种嗜热蛋白酶,其具有以下物理化学性质:(a)比活性和底物特异性:蛋白酶作用于酪蛋白,明胶,血红蛋白和白蛋白以特异性分解它们,底物特异性按酪蛋白,明胶的顺序降低 ,血红蛋白和白蛋白; (b)最佳pH:8.0; (c)pH稳定性:蛋白酶在30℃下在pH 6.5至pH 10.0范围内稳定1小时; (d)最佳温度:约40℃。 (e)温度稳定性:在pH7下1小时,蛋白酶在高达30℃的温度下是活性的,但在40℃下灭活达40%左右,并在50℃完全失活。 约10分钟; (f)酶活性:蛋白酶在约20℃下具有50%以上的最大活性; (g)酶的活性中心是丝氨酸; 和(h)通过SDS-PAGE测量,蛋白酶的分子量为约70kDa。
    • 78. 发明授权
    • Hydrocarbon emulsifier or solubilizer composition produced by
Flavobacterium FERM BP-4010
    • 由Flavobacterium FERM BP-4010生产的烃类乳化剂或增溶剂组合物
    • US5518726A
    • 1996-05-21
    • US242853
    • 1994-05-16
    • Kazuhito MoriyaKoki Horikoshi
    • Kazuhito MoriyaKoki Horikoshi
    • C02F1/40C02F3/00C02F3/34C12N1/20C12R1/20E02B15/10A01N63/00C12N1/00C12N1/26
    • C12R1/20C02F3/34Y10S435/85
    • A microorganism belonging to the genus Flavobacterium posessing the capacity to decompose hydrocarbons, tolerance to sulfurous acids, tolerance to salinity, tolerance to organic solvents, and tolerance to pressure. The microorganism is of a strain of the genus Flavobacterium DS-711 (FERM BP-4010). A hydrocarbon emulsifier or solubilizer having as an active component thereof a water soluble and acetone insoluble fraction obtained by culturing DS-711 strain. In addition the emulsifier or solubilizer obtained by culturing the strain DS-711 contains 18.4% protein, 18.8% carbohydrates and 28.6% lipids. A separation method for an organic-solvent tolerant microorganism, wherein a sample is mixed with water and an organic solvent, shaking culturing is conducted, a cultured mixture is allowed to stand, separation into an aqueous phase and an organic solvent phase is conducted, an appropriate amount of said organic solvent phase is added to a culture medium and cultured, and microorganisms which grow therein are isolated.
    • 属于属于分解碳氢化合物的能力的微生物的微生物,对亚硫酸的耐受性,对盐度的耐受性,对有机溶剂的耐受性和耐受性。 该微生物是属于属于黄杆菌属DS-711(FERM BP-4010)的菌株。 具有作为其活性成分的烃乳化剂或增溶剂是通过培养DS-711菌株获得的水溶性和丙酮不溶性级分。 此外,通过培养菌株DS-711获得的乳化剂或增溶剂含有18.4%的蛋白质,18.8%的碳水化合物和28.6%的脂质。 一种有机溶剂耐受微生物的分离方法,其中样品与水和有机溶剂混合,进行振荡培养,使培养的混合物静置,分离成水相,并进行有机溶剂相, 将适量的所述有机溶剂相加入到培养基中并进行培养,分离生长在其中的微生物。