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72. 发明申请

US20010006416A1 Ribbon flow cytometry apparatus and methods 审中-公开
标题翻译：带状流式细胞术仪器及方法
公开(公告)号：US20010006416A1
公开(公告)日：2001-07-05
申请号：US09770883
申请日：2001-01-26
发明人： Paul E. Johnson
IPC分类号： G01N021/00
CPC分类号： G01N15/14 , G01N2015/1411 , G01N2015/145
摘要： Increased speed in the detection of target particles in flow cytometry is accomplished using two-dimensional detectors to detect target particles in a thin ribbon flow. The thickness of the ribbon flow of the sample stream is coordinated with the depth of field of the detection system optics, allowing a sharp image of the target particles. The width of the ribbon flow is coordinated with the field of view of the optics. The relatively large cross-sectional area of the flow in the ribbon flow chamber allows for a flow cytometer design not requiring a sheath flow. Signal-to-noise ratio (SNR) is greatly improved by using time delayed integration (TDI) in conjunction with the ribbon flow illumination to lower background noise from unwanted photons and CCD readout noise.
摘要翻译：在流式细胞术中检测靶颗粒的速度提高使用二维检测器来检测薄带流中的目标颗粒。样品流的色带流的厚度与检测系统光学元件的景深协调，允许目标颗粒的清晰图像。带状流的宽度与光学器件的视场相协调。带状流动室中流动的相对大的横截面面积允许不需要鞘流的流式细胞仪设计。通过使用时间延迟积分（TDI）结合色带流照明降低不需要的光子的背景噪声和CCD读出噪声，可以大大提高信噪比（SNR）。

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