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71. 发明申请

US20140296109A1 USING POPULATIONS OF BEADS FOR THE FABRICATION OF ARRAYS ON SURFACES 有权
标题翻译：使用人造丝来制作表面阵列
公开(公告)号：US20140296109A1
公开(公告)日：2014-10-02
申请号：US14204329
申请日：2014-03-11
申请人： Illumina Cambridge Limited
发明人： Roberto Rigatti , Geoffrey Paul Smith , Jonathan Mark Boutell
IPC分类号： C12Q1/68
CPC分类号： C12N15/1068 , B01J19/0046 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/537 , C12Q2563/149 , C12Q2563/161
摘要： The present invention provides methods for creating an array of features on a surface based on content transferred from a plurality of beads to the surface. Nucleic acid content can be transferred using a method including the steps of (a) providing a surface having one or more primer oligonucleotides attached to the surface; (b) providing a pool of beads, wherein beads in the pool have a plurality of templates attached thereto, the plurality comprising multiple copies of a single nucleic acid template sequence; (c) arraying the beads onto the surface by hybridizing the templates to the primer oligonucleotides; and (d) extending the primers to produce copies of the templates attached to the surface.
摘要翻译：本发明提供了基于从多个珠粒转移到表面的内容来在表面上创建特征阵列的方法。可以使用包括以下步骤的方法转移核酸含量：（a）提供具有附着于所述表面的一种或多种引物寡核苷酸的表面; （b）提供珠池，其中所述池中的珠子具有连接到其上的多个模板，所述多个模板包含单个核酸模板序列的多个拷贝; （c）通过将模板与引物寡核苷酸杂交将珠子排列在表面上; 和（d）扩展引物以产生附着在表面上的模板的拷贝。

72. 发明申请

US20140212871A1 Nucleic Acid Extraction from Heterogeneous Biological Materials 审中-公开
标题翻译：来自异质生物材料的核酸提取
公开(公告)号：US20140212871A1
公开(公告)日：2014-07-31
申请号：US14116372
申请日：2012-05-11
申请人： Wayne Comper , Leileata M. Russo , Johan Karl Olov Skog
发明人： Wayne Comper , Leileata M. Russo , Johan Karl Olov Skog
IPC分类号： C12N15/10
CPC分类号： C12N15/1003 , C07H1/08 , C07H21/02 , C12N15/1006 , C12N15/101 , C12Q1/6806 , C12Q2527/127 , C12Q2563/161
摘要： Methods for extracting high quality nucleic acids from a heterogenous collection of nucleic acid-containing materials from a biological sample are disclosed. The heterogenous collection of nucleic-acid containing materials may contain cells or microvesicles, or both. The extractions obtained by the methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis, or therapy evaluation for a medical condition.
摘要翻译：公开了从生物样品的含有核酸的材料的异质收集中提取高质量核酸的方法。含有核酸的材料的异质收集可能含有细胞或微泡，或两者。通过本文描述的方法获得的提取物的特征在于高产率和高完整性，使得提取的核酸可用于优先选择高质量核酸提取的各种应用，例如用于医疗状况的诊断，预后或治疗评估。

73. 发明申请

US20140045915A1 CANCER-RELATED BIOLOGICAL MATERIALS IN MICROVESICLES 审中-公开
标题翻译：与微生物相关的癌症相关生物材料
公开(公告)号：US20140045915A1
公开(公告)日：2014-02-13
申请号：US13819539
申请日：2011-08-31
申请人： Johan Karl Olov Skog , Leonora Balaj , Mikkel Noerholm , Xandra O. Breakefield
发明人： Johan Karl Olov Skog , Leonora Balaj , Mikkel Noerholm , Xandra O. Breakefield
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/118 , C12Q2600/154 , C12Q2600/156 , C12Q2600/158 , C12Q2600/178 , C12Q2563/161
摘要： Disclosed herein are methods for assaying a biological sample from a subject by analyzing components of microvesicle fractions in aid of risk, diagnosis, prognosis or monitoring of or directing treatment of the subject for, a disease or other medical condition in the subject. Also disclosed are methods of treatment and identifying biomarkers using a microvesicle fraction of a subject. Kits, pharmaceutical compositions, and profiles related to the methods are also disclosed.
摘要翻译：本文公开了通过分析微泡级分的组分来协助受试者的疾病或其他医学状况的风险，诊断，预后或监测或指导治疗的受试者的生物样品的方法。还公开了使用受试者的微泡级分的治疗和鉴定生物标志物的方法。还公开了与方法相关的试剂盒，药物组合物和特征。

74. 发明申请

US20130178397A1 USING POPULATIONS OF BEADS FOR THE FABRICATION OF ARRAYS ON SURFACES 有权
标题翻译：使用人造丝来制作表面阵列
公开(公告)号：US20130178397A1
公开(公告)日：2013-07-11
申请号：US13801144
申请日：2013-03-13
申请人： Roberto Rigatti , Geoffrey Paul Smith , Jonathan Mark Boutell
发明人： Roberto Rigatti , Geoffrey Paul Smith , Jonathan Mark Boutell
IPC分类号： B01J19/00
CPC分类号： C12N15/1068 , B01J19/0046 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/537 , C12Q2563/149 , C12Q2563/161
摘要： The present invention provides a method of creating an array of features. The method can include steps of (a) providing a plurality of beads, wherein each bead in the plurality of beads includes probe content; (b) contacting the plurality of beads with a surface to produce a layer of beads on the surface; and (c) transferring the probe content from the beads to the surface to create an array of spatially discrete features on the surface, wherein each spatially discrete feature includes probe content from a bead in the plurality of beads.
摘要翻译：本发明提供了一种创建特征阵列的方法。该方法可以包括以下步骤：（a）提供多个珠，其中多个珠中的每个珠包括探针含量; （b）使多个珠粒与表面接触以在表面上产生一层珠粒; 和（c）将探针含量从珠粒转移到表面以在表面上产生空间上离散的特征的阵列，其中每个空间上离散的特征包括来自多个珠粒中的珠粒的探针含量。

75. 发明申请

US20130052647A1 METHODS FOR FRACTIONATING AND PROCESSING MICROPARTICLES FROM BIOLOGICAL SAMPLES AND USING THEM FOR BIOMARKER DISCOVERY 审中-公开
标题翻译：从生物样品中分离和处理微生物并将其用于生物识别发现的方法
公开(公告)号：US20130052647A1
公开(公告)日：2013-02-28
申请号：US13578464
申请日：2011-02-10
申请人： Marianna Goldrick , Lance Ford
发明人： Marianna Goldrick , Lance Ford
IPC分类号： C12Q1/68 , C07K1/14 , C12S3/18 , C07H1/08 , C12Q1/02 , C12N5/00
CPC分类号： C07K1/34 , C12Q1/6806 , C12Q2563/161
摘要： Described herein are methods, devices, and compositions for fractionation and processing of microparticles from biological samples, and to methods for obtaining and using the microparticles for biomarker discovery. Biological samples include cell-free fluids, for example blood plasma, blood serum, cerebrospinal fluid, urine, and saliva, as well as conditioned media. Conditioned media is the liquid growth media used to propagate cells in vitro. Purification of microparticles from cell-free fluids is challenging, typically accomplished by prolonged ultracentrifugation. Described herein is an alternative method for efficiently harvesting and processing microparticles from cell-free fluids and from conditioned media. Embodiments described herein relate to use of the microparticles and their contents recovered from conditioned media derived from propagation of human and animal cells, as a source of biomarkers for diagnosis and prognosis of diseases and pathological conditions.
摘要翻译：本文描述了用于从生物样品中分离和加工微粒的方法，装置和组合物，以及获得和使用微粒用于生物标志物发现的方法。生物样品包括无细胞流体，例如血浆，血清，脑脊髓液，尿液和唾液以及条件培养基。条件培养基是用于在体外繁殖细胞的液体生长培养基。从无细胞流体中纯化微粒是有挑战性的，通常通过长时间超速离心来实现。本文描述的是用于从无细胞流体和条件培养基有效收获和加工微粒的替代方法。本文所述的实施方案涉及从源自人和动物细胞繁殖的条件培养基中回收的微粒及其内容物作为疾病和病理状态的诊断和预后的生物标志物的来源。

76. 发明申请

US20120264622A1 COMBINATORIAL DECODING OF RANDOM NUCLEIC ACID ARRAYS 有权
标题翻译：随机核酸阵列的组合解码
公开(公告)号：US20120264622A1
公开(公告)日：2012-10-18
申请号：US13532606
申请日：2012-06-25
申请人： Mark Chee , David R. Walt
发明人： Mark Chee , David R. Walt
IPC分类号： C40B20/04
CPC分类号： C12Q1/6874 , B01J2219/005 , B01J2219/00572 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , C07B2200/11 , C07H21/00 , C12Q1/6837 , C40B20/04 , C40B40/06 , C40B70/00 , C12Q2563/161 , C12Q2563/185 , C12Q2525/179 , C12Q2563/155 , C12Q2565/514
摘要： Methods disclosed herein relate to identification of nucleotides in a nucleotide sequence.
摘要翻译：本文公开的方法涉及鉴定核苷酸序列中的核苷酸。

77. 发明申请

US20110251101A1 COMBINATORIAL DECODING OF RANDOM NUCLEIC ACID ARRAYS 有权
标题翻译：随机核酸阵列的组合解码
公开(公告)号：US20110251101A1
公开(公告)日：2011-10-13
申请号：US13159302
申请日：2011-06-13
申请人： Mark Chee , David R. Walt
发明人： Mark Chee , David R. Walt
IPC分类号： C40B30/04
CPC分类号： C12Q1/6874 , B01J2219/005 , B01J2219/00572 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , C07B2200/11 , C07H21/00 , C12Q1/6837 , C40B20/04 , C40B40/06 , C40B70/00 , C12Q2563/161 , C12Q2563/185 , C12Q2525/179 , C12Q2563/155 , C12Q2565/514
摘要： Methods disclosed herein relate to identification of nucleotides in a nucleotide sequence.
摘要翻译：本文公开的方法涉及鉴定核苷酸序列中的核苷酸。

78. 发明申请

US20090286688A1 COMBINATORIAL DECODING OF RANDOM NUCLEIC ACID ARRAYS 有权
标题翻译：随机核酸阵列的组合解码
公开(公告)号：US20090286688A1
公开(公告)日：2009-11-19
申请号：US12506116
申请日：2009-07-20
申请人： Mark Chee , David R. Walt
发明人： Mark Chee , David R. Walt
IPC分类号： C40B20/04
CPC分类号： C12Q1/6874 , B01J2219/005 , B01J2219/00572 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , C07B2200/11 , C07H21/00 , C12Q1/6837 , C40B20/04 , C40B40/06 , C40B70/00 , C12Q2563/161 , C12Q2563/185 , C12Q2525/179 , C12Q2563/155 , C12Q2565/514
摘要： Methods disclosed herein relate to identification of nucleotides in a nucleotide sequence.
摘要翻译：本文公开的方法涉及鉴定核苷酸序列中的核苷酸。

79. 发明申请

US20090182137A1 Vesicle for separating substances from liquid media 审中-公开
标题翻译：用于从液体介质中分离物质的囊泡
公开(公告)号：US20090182137A1
公开(公告)日：2009-07-16
申请号：US10588205
申请日：2005-02-02
申请人： Ulrich Schwaneberg , Mathias Winterhalter , Wolfgang Meier
发明人： Ulrich Schwaneberg , Mathias Winterhalter , Wolfgang Meier
IPC分类号： C07H1/00 , C12N1/00
CPC分类号： C12N15/1006 , C12Q2563/119 , C12Q2563/161
摘要： The invention relates to methods of separating substances from liquid media and to separating agents suitable therefor.
摘要翻译：本发明涉及从液体介质和分离剂分离物质的方法。

80. 发明申请

US20050214805A1 Methods of detection employing immuno-Q-Amp technology 审中-公开
标题翻译：使用免疫Q-Amp技术的检测方法
公开(公告)号：US20050214805A1
公开(公告)日：2005-09-29
申请号：US10985183
申请日：2004-11-09
申请人： Abraham Grossman
发明人： Abraham Grossman
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/567 , G01N33/58 , G01N33/68
CPC分类号： G01N33/58 , C12Q1/6867 , G01N33/6896 , G01N2800/2828 , C12Q2563/161
摘要： Disclosed herein are methods of detecting target molecules from a complex sample using a modified affinity ligand. In particular, the modified affinity ligand is an antibody specific for the target and has conjugated to it a nucleic acid molecule. This conjugated nucleic acid molecule serves as a template for a replicase that can replicate the template a billion-fold in an matter of minutes. The replication products then serve to facilitate a detectable signal indicating the presence of the target in the sample.
摘要翻译：本文公开了使用修饰的亲和配体从复合物样品检测靶分子的方法。特别地，修饰的亲和配体是对靶标具有特异性的抗体，并与其缀合核酸分子。该缀合的核酸分子用作复制酶的模板，其可以在几分钟内复制模板十亿倍。然后复制产物用于促进指示样品中目标物的存在的可检测信号。

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