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71. 发明申请

US20100278782A1 DIFFERENTIATION OF RAT LIVER EPITHELIAL CELLS INTO HEPATOCYTE-LIKE CELLS 审中-公开
标题翻译：大鼠肝上皮细胞分化为类细胞样细胞的分化
公开(公告)号：US20100278782A1
公开(公告)日：2010-11-04
申请号：US12304117
申请日：2007-06-12
申请人： Vera Rogiers , Tamara Vanhaecke , Sarah Snykers , Peggy Papeleu , Mathieu Vinken , Tom Henkens , Evelien De Rop , Joanna Fraczek , Joery De Kock , Tatyana Doktorova
发明人： Vera Rogiers , Tamara Vanhaecke , Sarah Snykers , Peggy Papeleu , Mathieu Vinken , Tom Henkens , Evelien De Rop , Joanna Fraczek , Joery De Kock , Tatyana Doktorova
IPC分类号： A61K35/12 , C12N5/07 , C12Q1/02 , A61P1/16
CPC分类号： C12N5/067 , C12N2500/25 , C12N2501/119 , C12N2501/12 , C12N2501/39
摘要： Methods for differentiation of cells of a rat liver epithelial (RLEC) cell line to hepatocyte-like cells are disclosed. In particular, methods involve sequential exposure of the RLEC to two or more differentiation agents. Cells obtained by the method are described as well as their uses in various pharmacological, toxicological, therapeutic, diagnostic and research applications.
摘要翻译：公开了将大鼠肝上皮（RLEC）细胞系细胞分化为肝细胞样细胞的方法。特别地，方法涉及RLEC连续暴露于两种或更多种分化剂。描述通过该方法获得的细胞以及它们在各种药理，毒理学，治疗，诊断和研究应用中的用途。

72. 发明申请

US20100136688A1 METHOD OF DIFFERENTIATING MAMMALIAN PROGENITOR CELLS INTO INSULIN PRODUCING PANCREATIC ISLET CELLS 有权
标题翻译：将MAMMALIAN PROGENITOR细胞分化成产生胰腺细胞的胰岛素的方法
公开(公告)号：US20100136688A1
公开(公告)日：2010-06-03
申请号：US12470247
申请日：2009-05-21
申请人： Joseph Charles RUIZ , Steven Michael HOYNOWSKI
发明人： Joseph Charles RUIZ , Steven Michael HOYNOWSKI
IPC分类号： C12N5/071
CPC分类号： C12N5/0676 , C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2500/90 , C12N2501/065 , C12N2506/02 , C12N2506/14 , C12N2509/00 , G01N33/5014 , G01N33/5038 , G01N33/507
摘要： The invention relates to methods for differentiating progenitor cells into insulin producing pancreatic islet cells and compositions and methods for using such cells.
摘要翻译：本发明涉及将祖细胞分化成胰岛素产生胰岛细胞的方法以及使用这种细胞的组合物和方法。

73. 发明申请

US20090317365A1 SYSTEMS AND METHODS FOR MAKING HEPATOCYTES FROM EXTRAHEPATIC SOMATIC STEM CELLS AND USE THEREOF 有权
标题翻译：用于从外来静脉干细胞制备肝细胞的系统和方法及其用途
公开(公告)号：US20090317365A1
公开(公告)日：2009-12-24
申请号：US12489760
申请日：2009-06-23
申请人： Oscar Kuang-Sheng LEE , Tom Kwang-Chun Kuo
发明人： Oscar Kuang-Sheng LEE , Tom Kwang-Chun Kuo
IPC分类号： A61K35/12 , C12N5/06 , C12Q1/02 , C12Q1/48 , C12Q1/42 , C12Q1/26
CPC分类号： G01N33/5067 , A61K35/407 , C12N5/067 , C12N2500/25 , C12N2500/38 , C12N2501/06 , C12N2501/113 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/155 , C12N2501/39 , C12N2503/02 , C12N2506/1353
摘要： A method for making hepatocytes from extrahepatic somatic stem cells comprises: a) culturing somatic stem cells in a medium comprising hepatic growth factor to cause the stem cells to differentiate toward hepatocytes; b) culturing cells from a) in a medium comprising HGF and oncostatin M to facilitate the cell differentiation toward hepatocytes; and c) culturing cells from b) in a medium comprising oncostatin M to cause the differentiated cells to mature into hepatocytes, thereby producing a cell population that has morphological features of hepatocytes and at least four of the following characteristics: i) antibody-detectable expression of albumin; ii) real-time reverse transcriptase-polymerase chain reaction-detectable expression of α-fetoprotein, HNF-1α, HNF-3β, HNF-4, HNF-6, α1-antitrypsin, alkaline phosphatase, tryptophan 2,3-dioxygenase, tyrosine aminotransferase, cytochrome P450 family 2 subfamily E polypeptide 1, glutamine synthetase, and/or low density lipoprotein receptor; iii) urea secretion; iv) cytochrome p450 enzyme activity; v) glycogen storage; and vi) low density lipoprotein uptake.
摘要翻译：从肝外体细胞干细胞制备肝细胞的方法包括：a）在包含肝细胞生长因子的培养基中培养体细胞干细胞，使干细胞分化成肝细胞; b）在包含HGF和制瘤素M的培养基中培养来自a）的细胞，以促进细胞向肝细胞分化; 和c）在包含制瘤素M的培养基中培养来自b）的细胞以使分化的细胞成熟为肝细胞，从而产生具有肝细胞形态特征和至少四种以下特征的细胞群：i）抗体可检测的表达白蛋白 ii）甲胎蛋白，HNF-1α，HNF-3β，HNF-4，HNF-6，α1-抗胰蛋白酶，碱性磷酸酶，色氨酸2,3-双加氧酶，酪氨酸的实时逆转录酶 - 聚合酶链反应可检测的表达氨基转移酶，细胞色素P450家族2亚科E多肽1，谷氨酰胺合成酶和/或低密度脂蛋白受体; iii）尿素分泌; iv）细胞色素p450酶活性; v）糖原储存; 和vi）低密度脂蛋白摄取。

74. 发明申请

US20090298170A1 HEPATOCYTE LINEAGE CELLS 有权
标题翻译： HEPATOCYTE线细胞
公开(公告)号：US20090298170A1
公开(公告)日：2009-12-03
申请号：US12298898
申请日：2007-04-27
申请人： Kevin D'Amour , Emmanuel E. Baetge
发明人： Kevin D'Amour , Emmanuel E. Baetge
IPC分类号： C12N5/08 , C12N5/00
CPC分类号： C12N5/0672 , C12N5/067 , C12N2501/115 , C12N2501/119 , C12N2501/155 , C12N2506/02
摘要： Disclosed herein are methods for producing liver precursor cells as well as hepatocyte cells form pluripotent and/or multipotent cells. Also disclosed herein are methods of enriching isolating and/or purifying liver precursor cells and/or hepatocyte cells. Further disclosed are compositions comprising cell cultures and cell populations that are enriched for liver precursor cells or hepatocyte cells.
摘要翻译：本文公开了用于产生肝前体细胞以及形成多潜能细胞和/或多能细胞的肝细胞细胞的方法。本文还公开了富集分离和/或纯化肝前体细胞和/或肝细胞细胞的方法。还公开了包含富含肝前体细胞或肝细胞细胞的细胞培养物和细胞群的组合物。

75. 发明申请

US20090221068A1 Cell Cultivation Method and Cell Culture 有权
标题翻译：细胞培养方法和细胞培养
公开(公告)号：US20090221068A1
公开(公告)日：2009-09-03
申请号：US11992746
申请日：2006-09-29
申请人： Naoya Kobayashi , Noriaki Tanaka
发明人： Naoya Kobayashi , Noriaki Tanaka
IPC分类号： C12N5/08 , C12N5/00 , C12M1/00
CPC分类号： C12N5/0671 , C12N5/067 , C12N5/0676 , C12N2533/50 , C12N2533/52 , C12N2533/54
摘要： Provided is a cell cultivation method in which the cell is cultured using a peptide hydrogel as a scaffold, for carrying out high-dimensional culture of a cell such as porcine hepatocyte, human hepatocyte, porcine pancreatic islet or human pancreatic islet for a long period under conditions where cell survival, cell morphology and cell functions are maintained. Also provided are a cell culture including a cell and a peptide hydrogel obtained by the above-described cultivation method, a bioreactor including the cell culture, and a cell preparation including the cell culture.
摘要翻译：提供一种细胞培养方法，其中使用肽水凝胶作为支架培养细胞，用于长时间进行细胞如猪肝细胞，人肝细胞，猪胰岛或人胰岛的高维培养保持细胞存活，细胞形态和细胞功能的条件。还提供了包括通过上述培养方法获得的细胞和肽水凝胶的细胞培养物，包含细胞培养物的生物反应器和包含细胞培养物的细胞制备物。

76. 发明授权

US07553663B2 Dedifferentiated, programmable stem cells of monocytic origin, and their production and use 有权
标题翻译：单分散来源的分化型可编程干细胞及其生产和使用
公开(公告)号：US07553663B2
公开(公告)日：2009-06-30
申请号：US11137444
申请日：2005-05-26
申请人： Bernd Karl Friedrich Kremer , Fred Fändrich , Maren née Schulze Ruhnke
发明人： Bernd Karl Friedrich Kremer , Fred Fändrich , Maren née Schulze Ruhnke
IPC分类号： C12N5/00
CPC分类号： A61L27/3886 , A61K35/12 , A61K2035/124 , A61L27/3804 , A61L27/3834 , A61L27/3895 , C12N5/0607 , C12N5/0618 , C12N5/0619 , C12N5/0622 , C12N5/0629 , C12N5/0647 , C12N5/0653 , C12N5/067 , C12N5/0676 , C12N5/069 , C12N2500/30 , C12N2500/44 , C12N2501/10 , C12N2501/11 , C12N2501/117 , C12N2501/119 , C12N2501/12 , C12N2501/20 , C12N2501/22 , C12N2501/23 , C12N2501/235 , C12N2501/33 , C12N2501/385 , C12N2501/39 , C12N2502/14 , C12N2506/03 , C12N2506/11 , C12N2510/00
摘要： The invention relates to the production of adult dedifferentiated, programmable stem cells from human monocytes by cultivation of monocytes in a culture medium which contains M-CSF and IL-3. The invention further relates to pharmaceutical preparations, which contain the dedifferentiated, programmable stem cells and the use of these stem cells for the production of target cells and target tissue.
摘要翻译：本发明涉及通过在含有M-CSF和IL-3的培养基中培养单核细胞从人单核细胞生产成人去分化的可编程干细胞。本发明还涉及含有去分化的可编程干细胞的药物制剂，以及这些干细胞用于产生靶细胞和靶组织的用途。

77. 发明申请

US20090011493A1 DEVICE CONTAINING CYTOPHILIC ISLANDS THAT ADHERE CELLS SEPARATED BY CYTOPHOBIC REGIONS 失效
标题翻译：含有CYPOPHOBIC区域分离细胞的胞质岛的装置
公开(公告)号：US20090011493A1
公开(公告)日：2009-01-08
申请号：US12166287
申请日：2008-07-01
申请人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory N. Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory N. Stephanopoulos
IPC分类号： C12M1/00
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
摘要翻译：本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。岛或区域或两者可以由自组装单层（SAM）形成。

78. 发明申请

US20080241926A1 CELL ADHESION ON SURFACES OF VARYING TOPOGRAPHIES 审中-公开
标题翻译：细胞粘附对变化的地形的表面
公开(公告)号：US20080241926A1
公开(公告)日：2008-10-02
申请号：US12041244
申请日：2008-03-03
申请人： Ilsoon Lee , Christina Chan , Srivatsan Kidambi
发明人： Ilsoon Lee , Christina Chan , Srivatsan Kidambi
IPC分类号： C12N5/06 , B05D3/00 , B05D1/36 , B32B5/00
CPC分类号： C12N5/0068 , C12N5/0656 , C12N5/067 , C12N2533/30 , C12N2535/10 , Y10T428/31
摘要： Micro-topography of a surface influences cell adhesion and proliferation. To improve adhesion, polyelectrolyte multilayers (PEMs) are built on patterned support layers to increase surface wettability, thereby improving attachment and spreading of the cells. Physical parameters, such as pattern size and pitch, in part, regulate cell adhesion and proliferation. Varying the surface topography provides a method to influence cell attachment and proliferation for tissue engineering applications.
摘要翻译：表面的微观形貌影响细胞粘附和增殖。为了改善粘附性，将聚电解质多层（PEM）构建在图案化支撑层上以增加表面润湿性，从而改善细胞的附着和扩散。物理参数，如图案大小和间距，部分调节细胞粘附和增殖。改变表面形貌提供了影响组织工程应用的细胞附着和增殖的方法。

79. 发明申请

US20080233642A9 MAMMALIAN IMMORTALIZED LIVER CELL 审中-公开
标题翻译： MAMMALIAN IMMURALIZED LIVER CELL
公开(公告)号：US20080233642A9
公开(公告)日：2008-09-25
申请号：US10169084
申请日：2002-03-15
申请人： Naoya Kobayashi , Philippe Leboulch , Noriaki Tanaka , Toshiyoshi Fujiwara , Toshinori Totsugawa
发明人： Naoya Kobayashi , Philippe Leboulch , Noriaki Tanaka , Toshiyoshi Fujiwara , Toshinori Totsugawa
IPC分类号： C12N5/08
CPC分类号： C12N5/067 , A61K35/12 , C12N2503/00 , C12N2503/02 , C12N2510/04
摘要： The present invention provides a mammalian immortalized liver cell obtained by transferring a cell proliferation factor gene located between a pair of site-specific recombination sequences into a mammalian liver cell.
摘要翻译：本发明提供通过将位于一对位点特异性重组序列之间的细胞增殖因子基因转移到哺乳动物肝细胞中而获得的哺乳动物永生化肝细胞。

80. 发明申请

US20080206343A1 Hepatocytes and Chondrocytes from Adherent Placental StemCells; And CD34+ ,CD45- Placental Stem Cell-Enriched Cell Populations 审中-公开
标题翻译：来自粘附胎盘干细胞的肝细胞和软骨细胞; 和CD34 +，CD45-胎盘干细胞富集细胞群体
公开(公告)号：US20080206343A1
公开(公告)日：2008-08-28
申请号：US12030170
申请日：2008-02-12
申请人： James W. Edinger , Robert J. Hariri , Jia-Lun Wang , Qian Ye , Marian Pereira , Sascha Dawn Abramson , Kristen S. Labazzo
发明人： James W. Edinger , Robert J. Hariri , Jia-Lun Wang , Qian Ye , Marian Pereira , Sascha Dawn Abramson , Kristen S. Labazzo
IPC分类号： A61K9/14 , C12N5/06 , A61K35/12 , A01K67/00 , C12Q1/70 , C12Q1/68 , C12N5/00
CPC分类号： C12N5/067 , A01K67/0271 , A01K2267/0337 , A61K2035/128 , C12N5/0605 , C12N5/0607 , C12N5/0655 , C12N2500/30 , C12N2500/36 , C12N2500/62 , C12N2501/15 , C12N2501/999 , C12N2506/02 , C12N2506/03 , C12N2533/32 , C12N2533/40 , C12N2533/74
摘要： Provided herein are methods and compositions for the production of hepatocytes from placenta stem cells. Further provided herein is the use of such hepatocytes in the treatment of, and intervention in, for example, trauma, inflammation, and degenerative disorders of the liver. Also provided herein are compositions and methods relating to combinations of nanofibrous scaffolds and adherent placental stem cells and methods of using the same in cartilage repair. Finally, provided herein are compositions and methods relating to nonadherent, CD34+CD45− stem cells from placenta.
摘要翻译：本文提供了用于从胎盘干细胞产生肝细胞的方法和组合物。本文进一步提供的是这种肝细胞在治疗和干预例如肝脏的创伤，炎症和退行性疾病中的用途。本文还提供了与纳米纤维支架和粘附性胎盘干细胞的组合相关的组合物和方法及其在软骨修复中的使用方法。最后，本文提供的是与来自胎盘的非粘附的CD34 + CD45 +干细胞相关的组合物和方法。

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