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71. 发明申请

US20090123917A1 METHODS FOR QUANTIFYING MICRORNA PRECURSORS 失效
标题翻译：定量微生物前体的方法
公开(公告)号：US20090123917A1
公开(公告)日：2009-05-14
申请号：US11816767
申请日：2006-02-24
申请人： Thomas D. Schmittigen
发明人： Thomas D. Schmittigen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/68 , C12Q2525/207
摘要： Provided herein is a sensitive, high throughput, real-time PCR assay to monitor the expression of miRNA precursors. Gene specific primers and reverse transcriptase were used to convert the primary precursors and pre-miRNAs to cDNA. The expression of 23 miRNA precursors in six human cancer cell lines was assayed using the PCR assay. The miRNA precursors accumulated to different levels when compared to each other or when a single precursor is compared in the various cell lines. The precursor expression profile of three miRNAs determined by the PCR assay was identical to the mature miRNA expression profile determined by Northern blotting. We propose that the PCR assay may be scaled up to include all of the 150+ known human miRNA genes and can easily be adaptable to other organisms such as plants, C. elegans and Drosophila.
摘要翻译：本文提供了一种灵敏，高通量的实时PCR测定法，用于监测miRNA前体的表达。基因特异性引物和逆转录酶用于将初级前体和前体miRNAs转化为cDNA。使用PCR测定法检测6种人类癌细胞系中23种miRNA前体的表达。当彼此相比时，或者当在各种细胞系中比较单个前体时，miRNA前体累积到不同水平。通过PCR测定确定的三种miRNA的前体表达谱与通过Northern印迹测定的成熟miRNA表达谱相同。我们建议PCR测定可以扩大到包括所有150多种已知的人类miRNA基因，并且可以容易地适应于其它生物如植物，秀丽隐杆线虫和果蝇。

72. 发明申请

US20090117542A1 UNIQUE SHORT TANDEM REPEATS AND METHODS OF THEIR USE 审中-公开
标题翻译：独特的短裤系列及其使用方法
公开(公告)号：US20090117542A1
公开(公告)日：2009-05-07
申请号：US11569324
申请日：2005-05-16
申请人： Julie L. Maybruck , Paul A. Fuerst
发明人： Julie L. Maybruck , Paul A. Fuerst
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6876 , C12Q2600/156
摘要： Methods for DNA fingerprinting identification of human DNA samples, comprising: a) exposing a DNA sample of an individual to at least one primer specific for a Y chromosome polymorphism at a predetermined loci, said loci being chosen from OSU9, OSU14, OSU22, OSU35, OSU51, OSU57, OSU67, OSU70, OSU73, OSU77, with the proviso that if OSU70 is selected then at least one other OSU locus is also selected; b) amplifying DNA of the DNA sample using the at least one primer specific for a Y chromosome polymorphism; and c) identifying the size of an amplified product. Primers for the methods are also provided.
摘要翻译：用于DNA指纹识别人DNA样本的方法，包括：a）将个体的DNA样品暴露于至少一个在预定位点处的Y染色体多态性特异性引物，所述位点选自OSU9，OSU14，OSU22，OSU35， OSU51，OSU57，OSU67，OSU70，OSU73，OSU77，条件是如果选择了OSU70，则还选择至少一个其他OSU轨迹; b）使用对于Y染色体多态性特异的至少一种引物扩增DNA样品的DNA; 和c）鉴定扩增产物的大小。还提供了方法的底漆。

73. 发明申请

US20090090162A1 Driver plate for electromagnetic forming of sheet metal 有权
标题翻译：钣金电磁成型用驱动板
公开(公告)号：US20090090162A1
公开(公告)日：2009-04-09
申请号：US11867734
申请日：2007-10-05
申请人： John R. Bradley , Glenn S. Daehn
发明人： John R. Bradley , Glenn S. Daehn
IPC分类号： B21D26/14 , B21D26/00
CPC分类号： B21D26/14 , Y10T29/49803
摘要： A multi-layer driver plate is disclosed for use in electromagnetic sheet metal forming operations. In one embodiment, the driver plate comprises a first layer characterized by low electrical resistivity and thickness for inducement and application of a suitable electromagnetic forming force, a second layer comprising an elastomeric material for compressing a sheet metal workpiece against a die surface and then regaining its original pre-forming structure, and a third layer interposed between the first layer and the second layer to protect the EMF force providing layer and to provide overall strength and durability to the EMF driver plate.
摘要翻译：公开了用于电磁钣金成型操作的多层驱动器板。在一个实施例中，驱动器板包括第一层，其特征在于用于诱导和施加合适的电磁成形力的低电阻率和厚度，第二层包括弹性体材料，用于将金属板工件压靠在模具表面上，然后重新获得原始预成形结构，以及插入在第一层和第二层之间的第三层，以保护EMF力提供层并为EMF驱动器板提供总体强度和耐久性。

74. 发明授权

US07504554B2 Animal model for identifying agents that inhibit or enhance CTLA4 signaling 有权
公开(公告)号：US07504554B2
公开(公告)日：2009-03-17
申请号：US11620935
申请日：2007-01-08
申请人： Yang Liu , Pan Zheng , Ping Lu , Bedrich Mosinger , Ken May
发明人： Yang Liu , Pan Zheng , Ping Lu , Bedrich Mosinger , Ken May
IPC分类号： G01N33/00
CPC分类号： A01K67/0278 , A01K67/0271 , A01K2207/15 , A01K2217/00 , A01K2217/072 , A01K2227/105 , A01K2267/025 , A01K2267/0325 , A01K2267/0337 , A01K2267/0356 , C07K14/70521 , C12N15/8509 , C12N2517/02 , C12N2800/30
摘要： The present invention relates to a non-human transgenic animal, particularly a knock in mouse, whose genome comprises a heterologous, chimeric CTLA4 gene. The chimeric CTLA4 gene comprises exon 2 of the human CTLA4 gene, exon 1 and exon 4 of the non-human animal, and exon 3 of the CTLA4 gene of the non-human animal, or preferably, exon 3 of the human CTLA4 gene. The invention also relates to methods by which the transgenic mice are used to screen for monoclonal antibodies or other molecules that enhance immunity to tumors and infectious agents by interacting with the human CTLA4 receptor. The transgenic mice of the present invention are also useful for screening for monoclonal antibodies or other molecules that inhibit autoimmunity and transplant rejection.

75. 发明申请

US20090048265A1 NOVEL ANTITUMOR AGENTS AND METHODS OF THEIR USE 有权
标题翻译：新型抗菌剂及其使用方法
公开(公告)号：US20090048265A1
公开(公告)日：2009-02-19
申请号：US11570966
申请日：2005-06-17
申请人： Ching-Shih Chen , Yeng-Jeng Shaw
发明人： Ching-Shih Chen , Yeng-Jeng Shaw
IPC分类号： A61K31/517 , C07D239/95 , C07D403/04 , A61P35/00
CPC分类号： A61K31/7072 , C07D239/95 , C07D405/12 , C07D409/12
摘要： Antitumor compounds based on the α1-adrenoceptor antagonist, doxazosin, as well as compositions and methods of use. The disclosed compounds induce apoptosis in cancer cells.
摘要翻译：基于α1-肾上腺素受体拮抗剂的抗肿瘤化合物，多沙唑嗪，以及组合物和使用方法。所公开的化合物诱导癌细胞凋亡。

76. 发明授权

US07437343B1 Multi-criterial decision making system and method 有权
标题翻译：多标准决策制度和方法
公开(公告)号：US07437343B1
公开(公告)日：2008-10-14
申请号：US11553126
申请日：2006-10-26
申请人： John R. Josephson , Balakrishnan Chandrasekaran , Mark Carroll , Naresh Sundaram Iyer
发明人： John R. Josephson , Balakrishnan Chandrasekaran , Mark Carroll , Naresh Sundaram Iyer
IPC分类号： G06F17/00 , G06N5/04
CPC分类号： G06Q10/06 , Y10S707/99933
摘要： An architecture is disclosed for assistance with exploration of design and other decision spaces and for making decisions. These decision spaces may be very large. The architecture consists of three main components: A Seeker acquires candidates by generating or retrieving them, along with their scores according to one or more criteria. A Filter locates a relatively small number of promising candidates that are retained for further analysis. Various filters may be used to locate the promising candidates. A Viewer allows a user to examine trade-off diagrams, and other linked displays, that present the filtered candidates for evaluation, analysis, further exploration, and narrowing the choice set. The computational load of the Seeker may be distributed among a large number of clients in a client-server computing environment.
摘要翻译：披露了一种架构，用于协助探索设计和其他决策空间以及作出决策。这些决策空间可能非常大。该架构由三个主要组成部分组成：寻求者根据一个或多个标准通过生成或检索它们以及其分数来获取候选者。过滤器定位了相对较少数量的有希望的候选人，用于进一步分析。可以使用各种滤波器来定位有希望的候选者。查看器允许用户检查权衡图和其他链接显示，呈现过滤的候选人进行评估，分析，进一步探索和缩小选择集。 Seeker的计算负载可以分布在客户端 - 服务器计算环境中的大量客户端之间。

77. 发明申请

US20080248575A1 Drug and Gene Delivery by Polymer Nanonozzle and Nanotip Cell Patch 审中-公开
标题翻译：药物和基因递送由聚合物Nanonozzle和Nanotip细胞贴片
公开(公告)号：US20080248575A1
公开(公告)日：2008-10-09
申请号：US12090958
申请日：2006-10-20
申请人： L. James Lee , Shengnian Wang , Yubing Xie , Changchun Zeng , Chee Guan Koh , Zhengzheng Fei
发明人： L. James Lee , Shengnian Wang , Yubing Xie , Changchun Zeng , Chee Guan Koh , Zhengzheng Fei
IPC分类号： C12N15/87 , B29C67/24 , C12N5/06
CPC分类号： C12N15/87
摘要： Delivery of drugs or genes to individual cells is achieved on a nanoscale using electroporation techniques. In one method, a flow-through bioreactor having an inlet and an outlet connected by a flow chamber and a nanoporous membrane positioned in the flow chamber is used. Cells to be electroporated are flowed from the inlet to the outlet, a quantum of molecules of the at least one drug or gene in a fluid medium in the flow chamber. An electrical field applied in the flow chamber provides momentum to the molecules in the nanopores, resulting in delivery of the molecules into the plurality of cells.
摘要翻译：使用电穿孔技术在纳米尺度上实现将药物或基因递送至个体细胞。在一种方法中，使用具有通过流动室连接的入口和出口以及位于流动室中的纳米孔膜的流通式生物反应器。要电穿孔的细胞从入口到出口流动，流动室中的流体介质中的至少一种药物或基因的分子的量子。施加在流动室中的电场为纳米孔中的分子提供动量，导致分子输送到多个细胞中。

78. 发明申请

US20080206205A1 Cd34(+) Cells And Their Methods Of Use 有权
标题翻译： Cd34（+）细胞及其使用方法
公开(公告)号：US20080206205A1
公开(公告)日：2008-08-28
申请号：US11908831
申请日：2006-03-20
申请人： Michael A. Caligiuri , Aharon G. Freud , Michael B. Becknell
发明人： Michael A. Caligiuri , Aharon G. Freud , Michael B. Becknell
IPC分类号： A61K35/12 , C12N5/06 , A61P43/00
CPC分类号： C12N5/0646 , C12N2501/23
摘要： A CD34(+) cell line, which can be found in human lymph nodes, called CD34dimCD45RA(+)integrinβ7bright. Methods of isolating the novel cell type from tissue and methods of its use are also provided.
摘要翻译：可以在人淋巴结中发现的CD34（+）细胞系称为CD34抗体CD45RA（+）/整联蛋白7 ^{明亮。还提供了从组织中分离新型细胞类型的方法及其使用方法。}

79. 发明申请

US20080146815A1 PDK-1/AKT SIGNALING INHIBITORS 有权
标题翻译： PDK-1 / AKT信号抑制剂
公开(公告)号：US20080146815A1
公开(公告)日：2008-06-19
申请号：US11864612
申请日：2007-09-28
申请人： Ching-Shih Chen
发明人： Ching-Shih Chen
IPC分类号： C07D257/04 , C07D231/10
CPC分类号： C07D231/12
摘要： A new class of phosphoinositide-dependent kinase-1 (PDK-1) inhibitors of formula I: wherein X is selected from the group consisting of alkyl and haloalkyl; Ar is an aryl radical selected from the group consisting of phenyl, biphenyl, naphthyl, anthryl, phenanthryl, and fluorenyl; and wherein Ar is optionally substituted with one or more radicals selected from the group consisting of halo, C1-C4 alkyl, C1-C4 haloalkyl, azido, C1-C4 azidoalkyl, aryl, alkylaryl, haloaryl, haloalkylaryl, and combinations thereof, and R is selected from the group consisting of nitrile, acetonitrile, ethylnitrile, propylnitrile, carboxamide, amidine, tetrazole, oxime, hydrazone, acetamidine, aminoacetamide, guanidine, and urea. Also provided are methods of using the compounds for the treatment and prevention of cancer in humans.
摘要翻译：新型的式I的磷酸肌醇依赖性激酶-1（PDK-1）抑制剂：其中X选自烷基和卤代烷基; Ar是选自苯基，联苯基，萘基，蒽基，菲基和芴基的芳基; 并且其中Ar任选被一个或多个选自以下的基团取代：卤素，C 1 -C 4烷基，C 1〜 C 4卤代烷基，叠氮基，C 1 -C 4 - 叠氮基烷基，芳基，烷基芳基，卤代芳基，卤代烷基芳基及其组合，并且选择R 腈，乙腈，丙腈，甲酰胺，脒，四唑，肟，腙，乙脒，氨基乙酰胺，胍和尿素组成的组。还提供了使用该化合物治疗和预防人类癌症的方法。

80. 发明授权

US07381862B2 Identification of soybeans having resistance to Phytophthora sojae 失效
标题翻译：鉴定具有抵抗疫霉疫苗的大豆
公开(公告)号：US07381862B2
公开(公告)日：2008-06-03
申请号：US10436376
申请日：2003-05-12
申请人： Steven St. Martin , Anne Dorrance , Kara Burnham , Ron Fioritto , David Francis
发明人： Steven St. Martin , Anne Dorrance , Kara Burnham , Ron Fioritto , David Francis
IPC分类号： A01H5/00 , A01H5/10 , A01H4/00 , A01H1/00 , C12N15/82
CPC分类号： C12Q1/6895 , A01H5/10 , C12Q2600/13 , C12Q2600/156
摘要： The invention provides soybean plants having a novel determinant, Rps8, for resistance to Phytophthora sojae. The invention also provides methods for identifying germplasms that are either heterozygous or homozygous for Rps8 using marker assisted selection. Genetic and enzymatic markers with known chromosomal loci that are associated with the Rps8 locus are used to confirm Rps8-derived Phytophthora sojae resistance in germplasms. Marker assisted selection also used when introgressing Rps8-derived soybean Phytophthora sojae resistance into non-resistant soybean germplasm or less resistant soybean germplasms.
摘要翻译：本发明提供了具有新型决定簇Rps8的大豆植物，用于抵抗疫霉属（Phytophthora sojae）。本发明还提供了使用标记辅助选择鉴定Rps8是杂合的或纯合的种质的方法。使用与Rps8基因座相关的已知染色体基因座的遗传和酶标记用于证实Rps8衍生的种质中的疫霉属抗性。标记辅助选择也用于将Rps8衍生的大豆疫霉属抗性引入非抗性大豆种质或耐药性较差的大豆种质中。

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