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71. 发明授权

US06686152B2 Methods employing oligonucleotide-binding e-tag probes 失效
标题翻译：使用寡核苷酸结合电子标签探针的方法
公开(公告)号：US06686152B2
公开(公告)日：2004-02-03
申请号：US09825247
申请日：2001-04-02
申请人： Sharat Singh , Huan Tian
发明人： Sharat Singh , Huan Tian
IPC分类号： C12P168
CPC分类号： C07H19/06 , C07H19/10 , C07H21/00 , C40B70/00 , G01N33/561 , Y10S435/81
摘要： Methods for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The methods include the use of electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. In practicing the methods, the target-binding moiety of the e-tag probes hybridizes to complementary target sequences followed by nuclease cleavage of the e-tag probes and release of detectable e-tags or e-tag reporters. The mixture is exposed to a capture agent which binds uncleaved and/or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.
摘要翻译：提供了多种检测已知选择的核苷酸靶序列的方法。检测涉及到识别标签作为目标识别的结果。所述方法包括使用电泳标签探针或电子标签探针，其包含与靶结合部分连接的检测区域和称为迁移率调节剂的迁移率限定区域。在实施该方法时，e-标签探针的靶结合部分与互补靶序列杂交，随后是e-标签探针的核酸酶切割和可检测的电子标签或电子标签记录的释放。将混合物暴露于捕获剂，其结合未切割和/或部分切割的e-标签探针，然后进行电泳分离。在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。

72. 发明授权

US06613525B2 Microfluidic apparatus and method for purification and processing 有权
标题翻译：微流控装置及其净化处理方法
公开(公告)号：US06613525B2
公开(公告)日：2003-09-02
申请号：US10051847
申请日：2002-01-17
申请人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
发明人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
IPC分类号： C12Q168
CPC分类号： G01N27/44743 , B01F13/0059 , B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0631 , B01L2200/0668 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0877 , B01L2400/0409 , B01L2400/0415 , B01L2400/0421 , G01N27/44791 , G01N33/5002 , G01N33/569
摘要： Integrated microfluidic devices comprising at least an enrichment channel (10) and a main electrophoretic flowpath (12) are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet (6). The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally.
摘要翻译：提供包括至少富集通道（10）和主电泳流路（12）的集成微流体装置。在主题集成装置中，富集通道和主电泳流路被定位成使得废液通过排出口（6）离开所述主电泳流路。主题装置可用于各种电泳应用，包括临床测定，基因组学和药物应用的高通量筛选，点对点护理体外诊断，分子遗传分析和核酸诊断，细胞分离和生物研究。

73. 发明授权

US06344326B1 Microfluidic method for nucleic acid purification and processing 有权
标题翻译：用于核酸纯化和加工的微流控法
公开(公告)号：US06344326B1
公开(公告)日：2002-02-05
申请号：US09501706
申请日：2000-02-10
申请人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
发明人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
IPC分类号： C07H2104
CPC分类号： G01N27/44743 , B01F13/0059 , B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0631 , B01L2200/0668 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0877 , B01L2400/0409 , B01L2400/0415 , B01L2400/0421 , G01N27/44791 , G01N33/5002 , G01N33/569
摘要： Integrated microfluidic devices comprising at least an enrichment channel (10) and a main electrophoretic flowpath (12) are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet (6). The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally.
摘要翻译：提供包括至少富集通道（10）和主电泳流路（12）的集成微流体装置。在主题集成装置中，富集通道和主电泳流路被定位成使得废液通过排出口（6）离开所述主电泳流路。主题装置可用于各种电泳应用，包括临床测定，基因组学和药物应用的高通量筛选，点对点护理体外诊断，分子遗传分析和核酸诊断，细胞分离和生物研究。

74. 发明授权

US5811311A Metal chelate containing compositions for use in chemiluminescent assays 失效
公开(公告)号：US5811311A
公开(公告)日：1998-09-22
申请号：US488228
申请日：1995-06-07
申请人： Sharat Singh , Edwin F. Ullman
发明人： Sharat Singh , Edwin F. Ullman
IPC分类号： G01N33/532 , C07D265/30 , C07D279/12 , C07D327/06 , C07D413/04 , C07F5/00 , C07F15/00 , C12Q1/26 , G01N21/78 , G01N33/53 , G01N33/533 , G01N33/542 , G01N33/543 , G01N33/58 , G01N33/546
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , C07D413/04 , G01N33/533 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/968 , Y10S436/80 , Y10S436/805 , Y10S436/808 , Y10T436/13
摘要： Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.

75. 发明授权

US5536834A Cyclic ether compounds 失效
标题翻译：环醚化合物
公开(公告)号：US5536834A
公开(公告)日：1996-07-16
申请号：US471131
申请日：1995-06-06
申请人： Sharat Singh , Edwin F. Ullman
发明人： Sharat Singh , Edwin F. Ullman
IPC分类号： G01N33/532 , C07D265/30 , C07D279/12 , C07D327/06 , C07D413/04 , C07F5/00 , C07F15/00 , C12Q1/26 , G01N21/78 , G01N33/53 , G01N33/533 , G01N33/542 , G01N33/543 , G01N33/58
CPC分类号： C07D265/30 , C07D279/12 , C07D327/06 , C07D413/04 , G01N33/533 , G01N33/54313 , G01N33/5436 , G01N33/58 , G01N33/582 , G01N33/585 , G01N33/586 , Y10S435/968 , Y10S436/80 , Y10S436/805 , Y10S436/808 , Y10T436/13
摘要： Compositions are disclosed comprising (a) a metal chelate wherein the metal is selected from the group consisting of europium, terbium, dysprosium, samarium osmium and ruthenium in at least a hexacoordinated state and (b) a compound having a double bond substituted with two aryl groups, an oxygen atom and an atom selected from the group consisting of oxygen, sulfur and nitrogen wherein one of the aryl groups is electron donating with respect to the other. Such composition is preferably incorporated in a latex particulate material. Methods and kits are also disclosed for determining an analyte in a medium suspected of containing the analyte. The methods and kits employ as one component a composition as described above.
摘要翻译：公开了包含（a）金属螯合物的组合物，其中金属选自铕，铽，镝，钐锇和钌至少六配位状态，和（b）具有被两个芳基取代的双键的化合物基团，氧原子和选自氧，硫和氮的原子，其中一个芳基相对于另一个赋予电子。这种组合物优选掺入胶乳颗粒材料中。还公开了用于测定怀疑含有分析物的培养基中的分析物的方法和试剂盒。所述方法和试剂盒采用如上所述的组合物作为一种组分。

76. 发明授权

US09250243B2 Drug selection for lung cancer therapy using antibody-based arrays 有权
标题翻译：使用基于抗体的阵列进行肺癌治疗的药物选择
公开(公告)号：US09250243B2
公开(公告)日：2016-02-02
申请号：US12172100
申请日：2008-07-11
申请人： Sharat Singh , Jeanne Harvey
发明人： Sharat Singh , Jeanne Harvey
IPC分类号： G01N33/574 , G01N33/50 , G01N33/53
CPC分类号： G01N33/57423 , G01N33/5041 , G01N33/5044 , G01N33/5082 , G01N2500/10 , G01N2800/52
摘要： The present invention provides compositions and methods for detecting the activation states of components of signal transduction pathways in tumor cells. Information on the activation states of components of signal transduction pathways derived from use of the invention can be used for cancer diagnosis, prognosis, and in the design of cancer treatments.
摘要翻译：本发明提供用于检测肿瘤细胞中信号转导途径的组分的激活状态的组合物和方法。关于使用本发明的信号转导途径的组分的激活状态的信息可用于癌症诊断，预后和癌症治疗的设计。

77. 发明授权

US08709733B2 Methods for diagnosing irritable bowel syndrome 有权
标题翻译：肠易激综合征诊断方法
公开(公告)号：US08709733B2
公开(公告)日：2014-04-29
申请号：US13352225
申请日：2012-01-17
申请人： Hua Gong , Shui Long Wang , Sharat Singh
发明人： Hua Gong , Shui Long Wang , Sharat Singh
IPC分类号： G01N33/53 , G01N31/00
CPC分类号： G01N33/573 , G01N33/6893 , G01N33/74 , G01N2800/065 , G01N2800/56 , Y10S436/809 , Y10T436/147777
摘要： The invention provides an ELISA assay for the determination of serum mast cell β-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of β-tryptase, histamine and/or prostaglandin E2.
摘要翻译：本发明提供了使用兔抗类胰蛋白酶作为捕获抗体和碱性磷酸酶缀合的G3作为检测抗体的ELISA测定法来测定血清肥大细胞和bgr-酪氨酸酶水平。使用发光底物CPSD增强测定灵敏度。还提供了通过检测血清中β-谷氨酸酶，组胺和/或前列腺素E2的水平来帮助诊断肠易激综合征的方法。

78. 发明授权

US08445215B1 Assays and methods for the detection of Crohn's disease 有权
标题翻译：检测克罗恩病的检测方法
公开(公告)号：US08445215B1
公开(公告)日：2013-05-21
申请号：US13190369
申请日：2011-07-25
申请人： Shui-Long Wang , Nicholas Chi-Kwan Ling , Sharat Singh
发明人： Shui-Long Wang , Nicholas Chi-Kwan Ling , Sharat Singh
IPC分类号： G01N33/53
CPC分类号： G01N33/564 , G01N2333/245 , G01N2800/065
摘要： The present invention provides devices and methods for aiding in the diagnosis of Crohn's disease. The devices and methods accurately identify whether a sample from an individual is associated with inflammatory bowel disease (IBD) or a clinical subtype thereof such as Crohn's disease (CD). The present invention is useful for determining whether a sample from an individual is an IBD sample, or differentiating between CD and ulcerative colitis (UC). Thus, the present invention provides an accurate diagnostic prediction of IBD or a clinical subtype thereof and prognostic information useful for guiding treatment decisions.
摘要翻译：本发明提供了用于帮助诊断克罗恩病的装置和方法。装置和方法准确地识别来自个体的样品是否与炎症性肠病（IBD）或其临床亚型如克罗恩病（CD）相关。本发明可用于确定来自个体的样品是IBD样品还是区分CD和溃疡性结肠炎（UC）。因此，本发明提供IBD或其临床亚型的准确诊断预测和用于指导治疗决定的预后信息。

79. 发明申请

US20120277109A1 PROXIMITY-MEDIATED ASSAYS FOR DETECTING ONCOGENIC FUSION PROTEINS 审中-公开
标题翻译：用于检测ONCGENIC融合蛋白的近似介导的测定
公开(公告)号：US20120277109A1
公开(公告)日：2012-11-01
申请号：US13361881
申请日：2012-01-30
申请人： Sharat Singh , Xinjun Liu
发明人： Sharat Singh , Xinjun Liu
IPC分类号： G01N33/574 , C40B30/04
CPC分类号： G01N33/57426 , G01N33/5011 , G01N33/574 , G01N33/5748 , G01N2800/52 , G01N2800/56
摘要： The present invention provides antibody-based arrays for detecting the activation state and/or total amount of one or a plurality of oncogenic fusion proteins in a biological sample such as whole blood or tumor tissue and methods of use thereof. In certain instances, the activation state and/or total amount of oncogenic fusion protein(s) present in a sample can be measured in combination with one or a plurality of signal transduction molecules. The compositions and methods of the present invention have the advantages of specificity associated with enzyme-linked immunosorbent assays, sensitivity associated with signal amplification, and high-throughput multiplexing associated with microarrays.
摘要翻译：本发明提供了用于检测生物样品如全血或肿瘤组织中的一种或多种致癌融合蛋白的活化状态和/或总量的基于抗体的阵列及其使用方法。在某些情况下，存在于样品中的致癌融合蛋白的活化状态和/或总量可以与一种或多种信号转导分子组合测量。本发明的组合物和方法具有与酶联免疫吸附测定相关的特异性，与信号放大相关的灵敏度以及与微阵列相关的高通量复合的优点。

80. 发明申请

US20120244558A1 METHODS FOR DIAGNOSING IRRITABLE BOWEL SYNDROME 有权
标题翻译：用于诊断可刺激性牙刷综合征的方法
公开(公告)号：US20120244558A1
公开(公告)日：2012-09-27
申请号：US13352225
申请日：2012-01-17
申请人： Hua Gong , Shui Long Wang , Sharat Singh
发明人： Hua Gong , Shui Long Wang , Sharat Singh
IPC分类号： G01N33/53 , G06F19/10
CPC分类号： G01N33/573 , G01N33/6893 , G01N33/74 , G01N2800/065 , G01N2800/56 , Y10S436/809 , Y10T436/147777
摘要： The invention provides an ELISA assay for the determination of serum mast cell β-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of β-tryptase, histamine and/or prostaglandin E2.
摘要翻译：本发明提供了使用兔抗类胰蛋白酶作为捕获抗体和碱性磷酸酶缀合的G3作为检测抗体的ELISA测定法来测定血清肥大细胞和bgr-酪氨酸酶水平。使用发光底物CPSD增强测定灵敏度。还提供了通过检测血清中β-谷氨酸酶，组胺和/或前列腺素E2的水平来帮助诊断肠易激综合征的方法。

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