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71. 发明申请

US20080295204A1 DGAT GENES FROM YARROWIA LIPOLYTICA FOR INCREASED SEED STORAGE LIPID PRODUCTION AND ALTERED FATTY ACID PROFILES IN SOYBEAN 有权
标题翻译：来自YARROWIA LIPOLYTICA的DGAT基因用于增加种子脂肪生产和改变的大豆脂肪酸分布
公开(公告)号：US20080295204A1
公开(公告)日：2008-11-27
申请号：US12126161
申请日：2008-05-23
申请人： Knut Meyer , William D. Hitz , Narendra S. Yadav , Howard Glenn Damude
发明人： Knut Meyer , William D. Hitz , Narendra S. Yadav , Howard Glenn Damude
IPC分类号： C12N15/54 , A01H5/00 , C12N5/10
CPC分类号： C12N15/8247 , C12N9/1029 , C12Y203/01158
摘要： Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from Yarrowia Lipolytica are used to achieve the increase in seed storage lipids.
摘要翻译：描述了与非转基因，无偏分离大豆种子的总脂肪酸含量相比，具有至少10％的总脂肪酸含量增加和改变的脂肪酸分布的转基因大豆种子。来自解脂耶氏酵母的DGAT基因用于实现种子储存脂质的增加。

72. 发明申请

US20080124786A1 Acyltrasferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts 失效
标题翻译：用于改变多不饱和脂肪酸的酰基转移酶和含油酵母中的油含量
公开(公告)号：US20080124786A1
公开(公告)日：2008-05-29
申请号：US11716162
申请日：2007-03-09
申请人： Narendra S. Yadav , Honggxiang Zhang
发明人： Narendra S. Yadav , Honggxiang Zhang
IPC分类号： C12N1/21 , C12N15/12 , C07K14/00 , C12N1/13 , C12N1/19 , C12N1/15
CPC分类号： C12P7/6481 , C12N9/1029 , C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Two acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, the genes encoding phophatidylcholine-diacylglycerol acyltransferase (PDAT) and diacylglycerol acyltransferase (DGAT2) have been isolated from Y. lipolytica. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
摘要翻译：提供两种酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω-脂肪酸的微生物油。具体地说，从解脂耶氏酵母中分离出编码磷脂酰胆碱 - 二酰基甘油酰基转移酶（PDAT）和二酰基甘油酰基转移酶（DGAT2）的基因。这些基因编码酵母中参与油生物合成的最终步骤的酶。预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。

73. 发明授权

US07267979B2 Method of controlling gene silencing using site specific recombination 失效
标题翻译：使用位点特异性重组控制基因沉默的方法
公开(公告)号：US07267979B2
公开(公告)日：2007-09-11
申请号：US10611748
申请日：2003-06-30
申请人： Narendra S. Yadav
发明人： Narendra S. Yadav
IPC分类号： C12N15/82 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8218 , C12N15/8213
摘要： This invention relates to methods of controlling gene silencing using site-specific recombination. A variety of constructs are provided which are useful for conditional or regulated gene silencing in plants, comprising a suite of constitutive, inducible, tissue-specific or developmental stage-specific promoters operably linked to target sequences (TS). Recombinase inversion or excision yields double-stranded TS RNA, which thereby functions to trigger endogenous gene silencing mechanisms. By matching promoters, responsive to various inducers, plant tissues or plant developmental states with the recombinase systems, transcriptional stop fragments or introns and target sequences, gene silencing of virtually any target sequence may be modulated at any plant development stage or in any plant generation. This is especially useful, when genes responsible for gene silencing are down-regulated to permit expression of particular transgenes at levels greater than permitted when gene silencing is activated.
摘要翻译：本发明涉及使用位点特异性重组来控制基因沉默的方法。提供了可用于植物中条件或调节基因沉默的各种构建体，其包含与靶序列（TS）可操作地连接的组合的一组组成型，诱导型组织特异性或发育阶段特异性启动子。重组酶反转或切除产生双链TS RNA，从而起到引发内源性基因沉默机制的作用。通过匹配启动子，响应于各种诱导剂，植物组织或植物发育状态与重组酶系统，转录终止片段或内含子和靶序列，几乎任何靶序列的基因沉默可以在任何植物发育阶段或任何植物生成中调节。当基因沉默的基因被下调以允许特异转基因在基因沉默被激活时大于许可的水平表达时，这是特别有用的。

74. 发明授权

US5952544A Fatty acid desaturase genes from plants 失效
标题翻译：来自植物的脂肪酸去饱和酶基因
公开(公告)号：US5952544A
公开(公告)日：1999-09-14
申请号：US244205
申请日：1994-08-26
申请人： John Browse , Luis Perez Grau , Anthony J. Kinney , John W. Pierce, Jr. , Anna M. Wierzbicki , Narendra S. Yadav
发明人： John Browse , Luis Perez Grau , Anthony J. Kinney , John W. Pierce, Jr. , Anna M. Wierzbicki , Narendra S. Yadav
IPC分类号： A01H5/00 , A01H5/10 , C11B1/00 , C11C3/00 , C12N5/10 , C12N9/00 , C12N9/02 , C12N15/09 , C12N15/53 , C12N15/82 , C12Q1/68
CPC分类号： C12N9/0083 , A01H5/10 , C12N15/8247 , C12Y114/19006
摘要： The preparation and use of nucleic acid fragments encoding fatty acid desaturase enzymes are described. The invention permits alteration of plant lipid composition. Chimeric genes incorporating such nucleic acid fragments with suitable regulatory sequences may be used to create transgenic plants with altered levels of unsaturated fatty acids.
摘要翻译： PCT No.PCT / US92 / 10284 Sec。 371日期1994年8月26日 102（e）日期1994年8月26日PCT提交1992年12月3日PCT公布。公开号WO93 / 11245 日期：1993年6月10日描述了编码脂肪酸去饱和酶的核酸片段的制备和使用。本发明允许改变植物脂质组成。使用合适的调控序列掺入此类核酸片段的嵌合基因可用于产生具有改变水平的不饱和脂肪酸的转基因植物。

75. 发明授权

US5945585A Specific for palmitoyl, stearoyl and oleoyl-alp thioesters nucleic acid fragments encoding acyl-acp thiosesterase enzymes and the use of these fragments in altering plant oil composition 失效
标题翻译：特异性用于编码酰基-β-硫代酯酶的棕榈酰，硬脂酰和油酰 - 阿尔卑斯硫酯核酸片段，以及这些片段在改变植物油组成中的用途
公开(公告)号：US5945585A
公开(公告)日：1999-08-31
申请号：US948176
申请日：1997-10-09
申请人： William Dean Hitz , Narendra S. Yadav
发明人： William Dean Hitz , Narendra S. Yadav
IPC分类号： C11B1/00 , C12N9/16 , C12N15/55 , C12N15/82 , C12P7/64 , A01H5/00 , C12N5/14 , C12N15/29 , C12N15/52
CPC分类号： C12P7/6463 , C11B1/00 , C12N15/8247 , C12N9/16
摘要： Isolated nucleic acid fragments encoding an acyl-ACP thioesterase enzyme which catalyzes the hydrolysis of palmitoyl, stearoyl and oleoyl-ACP thioesters are described. Use of such fragments in altering plant oil composition is also described.
摘要翻译：描述了编码催化棕榈酰，硬脂酰和油酰-ACP硫酯水解的酰基-ACP硫酯酶的分离的核酸片段。还描述了在改变植物油组合物中使用这种片段。

76. 发明授权

US5443974A Nucleotide sequence of soybean stearoyl-ACP desaturase gene 失效
标题翻译：大豆硬脂酰-ACP去饱和酶基因的核苷酸序列
公开(公告)号：US5443974A
公开(公告)日：1995-08-22
申请号：US995657
申请日：1992-12-11
申请人： William D. Hitz , Narendra S. Yadav , Luis Perez-Grau
发明人： William D. Hitz , Narendra S. Yadav , Luis Perez-Grau
IPC分类号： A01H1/04 , A01H5/10 , C11B1/04 , C12N9/02 , C12N15/53 , C12N15/82 , C12Q1/68 , C12N15/00 , C12N15/05 , C12N15/11
CPC分类号： C12N9/0083 , A01H5/10 , C11B1/04 , C12N15/8247 , C12Q1/6895 , C12Y114/19002 , C12Q2600/156
摘要： The preparation and use of nucleic acid fragments encoding soybean seed stearoyl-ACP desaturase enzyme or its precursor to modify plant oil composition are described. Chimeric genes incorporating such nucleic acid fragments and suitable regulatory sequences may be utilized to transform plants to control the levels of saturated and unsaturated fatty acids.
摘要翻译：描述了编码大豆种子硬脂酰-ACP去饱和酶或其前体以修饰植物油组成的核酸片段的制备和用途。掺入此类核酸片段的嵌合基因和合适的调节序列可用于转化植物以控制饱和和不饱和脂肪酸的含量。

77. 发明授权

US5013659A Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase 失效
标题翻译：编码抗除草剂植物乙酰乳酸合成酶的核酸片段
公开(公告)号：US5013659A
公开(公告)日：1991-05-07
申请号：US164360
申请日：1988-03-04
申请人： John R. Bedbrook , Roy S. Chaleff , Saverio C. Falco , Barbara J. Mazur , Christopher R. Somerville , Narendra S. Yadav
发明人： John R. Bedbrook , Roy S. Chaleff , Saverio C. Falco , Barbara J. Mazur , Christopher R. Somerville , Narendra S. Yadav
IPC分类号： C12N9/88 , C12N15/82
CPC分类号： C12N9/88 , C12N15/8278
摘要： A nucleic acid fragment encoding a herbicide-resistant plant acetolactate synthase protein is disclosed. This nucleic acid fragment contains at least one nucleotide mutation resulting in one amino acid change in one of seven substantially conserved regions of acetolactate synthase amino acid homology. This mutation results in the production of an acetolactate synthase protein which is resistant to sulfonylurea herbicide compounds compared to the wild-type protein. Transformation of herbicide sensitive plants or plant cells with the fragment results in resistance to the herbicide.
摘要翻译：公开了编码除草剂抗性植物乙酰乳酸合酶蛋白的核酸片段。该核酸片段含有至少一个核苷酸突变，导致乙酰乳酸合酶氨基酸同源性的七个基本上保守的区域之一发生一个氨基酸变化。与野生型蛋白质相比，该突变导致对磺酰脲类除草剂化合物具有抗性的乙酰乳酸合酶蛋白质的产生。用片段转化除草剂敏感植物或植物细胞导致对除草剂的抗性。

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