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71. 发明申请

US20100257778A1 PRODUCTION OF FATTY ACID DERIVATIVES 审中-公开
标题翻译：生产脂肪酸衍生物
公开(公告)号：US20100257778A1
公开(公告)日：2010-10-14
申请号：US12758001
申请日：2010-04-11
申请人： Alfred Gaertner , Andreas Schirmer , Fernando Valle , Stephen Del Cardayre
发明人： Alfred Gaertner , Andreas Schirmer , Fernando Valle , Stephen Del Cardayre
IPC分类号： C10L1/19 , C12P7/62 , C07C69/003 , C12P7/64 , C12N1/00 , C12N1/21 , C07C53/00
CPC分类号： C10L1/026 , C10L2200/0476 , C10L2270/026 , C10L2290/26 , C12P7/649 , Y02E50/13 , Y02P30/20
摘要： Methods and compositions for producing fatty acid derivatives, for example, fatty esters, are described.
摘要翻译：描述了用于生产脂肪酸衍生物的方法和组合物，例如脂肪酸酯。

72. 发明申请

US20090203102A1 COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE 有权
标题翻译：用于生产异丙苯的组合物和方法
公开(公告)号：US20090203102A1
公开(公告)日：2009-08-13
申请号：US12335071
申请日：2008-12-15
申请人： Marguerite A. Cervin , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph C. Mcauliffe , Frank J. Feher , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
发明人： Marguerite A. Cervin , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph C. Mcauliffe , Frank J. Feher , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
IPC分类号： C12P5/02 , C12N1/20 , C12N1/21
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
摘要翻译：本发明的特征在于从培养细胞产生异戊二烯的方法。本发明还提供包括这些培养细胞的组合物。

73. 发明申请

US20080293124A1 Multimeric Oxidoreductases 有权
标题翻译：多聚氧化还原酶
公开(公告)号：US20080293124A1
公开(公告)日：2008-11-27
申请号：US11872890
申请日：2007-10-16
申请人： Robert M. Caldwell , M. Harunur Rashid , Fernando Valle
发明人： Robert M. Caldwell , M. Harunur Rashid , Fernando Valle
IPC分类号： C12N1/20 , C12N15/11 , C12N15/00
CPC分类号： C12P7/58 , C12N9/0004 , C12N9/002 , C12N15/74 , C12N15/78 , C12P7/60
摘要： The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.
摘要翻译：本发明涉及在碳底物的酶促转化中起作用的多聚体氧化还原酶复合物，所述复合物具有脱氢酶亚基和细胞色素C亚基。本发明还涉及编码多聚体复合物的多核苷酸及其使用方法。

74. 发明申请

US20070184540A1 Metabolically engineered bacterial strains having enhanced 2-keto-D-gluconate accumulation 审中-公开
标题翻译：具有增强的2-酮-D-葡萄糖酸盐积累的代谢改造的细菌菌株
公开(公告)号：US20070184540A1
公开(公告)日：2007-08-09
申请号：US11731923
申请日：2007-04-02
申请人： Timothy Dodge , M. Rashid , Fernando Valle
发明人： Timothy Dodge , M. Rashid , Fernando Valle
IPC分类号： C12P7/60 , C12N9/02 , C12N1/21 , C12N15/74
CPC分类号： C12P7/60 , C12N9/0006 , C12P19/02
摘要： The present invention relates to a method of altering bacterial host cells to accumulate 2-keto-D-gluconic acid (2-KDG) by inactivating an endogenous membrane bound 2-keto-D-gluconate dehydrogenase (2-KDGDH), which prior to inactivation catalyzed the conversion of 2-KDG to 2,5-diketogluconate (2,5-DKG).
摘要翻译：本发明涉及通过灭活内源性膜结合的2-酮-D-葡萄糖酸脱氢酶（2-KDGDH）来改变细菌宿主细胞以积累2-酮-D-葡萄糖酸（2-KDG）的方法，其在失活催化了2-KDG转化为2,5-二酮葡萄糖酸盐（2,5-DKG）。

75. 发明授权

US07229811B2 2,5-diketo-D-gluconic acid (2,5-DKG) permeases 有权
标题翻译： 2,5-二酮-D-葡萄糖酸（2,5-DKG）渗透酶
公开(公告)号：US07229811B2
公开(公告)日：2007-06-12
申请号：US10343369
申请日：2001-08-03
申请人： Manoj Kumar , Fernando Valle , Veronique A. Dartois , James A. Hoch
发明人： Manoj Kumar , Fernando Valle , Veronique A. Dartois , James A. Hoch
IPC分类号： C07H21/04 , C12N15/00 , C12N1/20 , C12P7/40 , C12P21/06 , C12Q1/26 , C07K14/00 , C12P19/34 , C12N9/04
CPC分类号： C07K14/26 , C07K14/24
摘要： The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease acitivity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.
摘要翻译：本发明提供了编码具有2,5-DKG通透酶活性的多肽的分离的核酸分子及其寡核苷酸。分离的核酸分子可以在适当的细菌细胞中表达以增强2-KLG的产生，随后将其转化为抗坏血酸。还提供了具有2,5-DKG通透酶活性的分离的多肽，其免疫原性肽及其特异的抗体。本发明还提供鉴定新型2,5-DKG通透酶的方法。

76. 发明授权

US07074608B1 Method for the production of 1,3-propanediol by recombinant organisms comprising genes for coenzyme B12 synthesis 有权
标题翻译：用于辅酶B12合成基因的重组生物制备1,3-丙二醇的方法
公开(公告)号：US07074608B1
公开(公告)日：2006-07-11
申请号：US09310001
申请日：1999-05-11
申请人： Nigel S. Dunn-Coleman , Anthony A. Gatenby , Fernando Valle
发明人： Nigel S. Dunn-Coleman , Anthony A. Gatenby , Fernando Valle
IPC分类号： C12N1/20
CPC分类号： C12N9/88 , C12P7/18 , C12Y402/0103
摘要： Recombinant organisms are provided comprising genes encoding aquacobalamin reductase, cob(II)alamin reductase, cob(I)alamin adenosyltransferase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates. More specifically the following nucleotide sequences are provided: btuR, encoding the E. coli cob(I)alamin adenosyltransferase enzyme; cobA, encoding the Salmonella typhimurium cob(I)alamin adenosyltransferase enzyme; cobO, encoding the Pseudomonas denitrificans cob(I)alamin adenosyltransferase enzyme; dhaB1, encoding the α subunit of the Klebsiella glycerol dehydratase enzyme; dhaB2, encoding the β subunit of the Klebsiella glycerol dehydratase enzyme; dhaB3, encoding the γ subunit of the Klebsiella glycerol dehydratase enzyme; dhaT, encoding Klebsiella oxidoreductase enzyme; the yciK gene isolated from E. coli; the glucose isomerase promoter sequence from Streptomyces; and the N-terminal amino acid sequence for cob(II)alamin reductase from Pseudomonas denitrificans.
摘要翻译：提供重组生物，其包含编码水产精氨酸还原酶，cob（II）氨基还原酶，cob（I）阿拉曼腺苷转移酶，甘油脱水酶和1,3-丙二醇氧化还原酶活性的基因，所述活性可用于从各种碳底物生产1,3-丙二醇。更具体地提供以下核苷酸序列：btuR，编码大肠杆菌（I）氨基腺苷酰基转移酶; cobA，编码鼠伤寒沙门氏菌（I）阿拉明腺苷转移酶; 编码脱氧核糖核酸假单胞菌（I）氨基腺苷酰基转移酶的cobO; dhaB1，编码克雷伯杆菌甘油脱水酶的α亚基; dhaB2，编码克雷伯氏菌甘油脱水酶的β亚基; dhaB3，编码克雷伯菌甘油脱水酶的γ亚基; dhaT，编码克雷伯杆菌氧化还原酶; 从大肠杆菌分离的yciK基因; 来自链霉菌的葡萄糖异构酶启动子序列; 和来自脱氮假单胞菌的cob（II）氨基还原酶的N-末端氨基酸序列。

77. 发明授权

US07033804B2 Methods for the production of products in host cells 有权
公开(公告)号：US07033804B2
公开(公告)日：2006-04-25
申请号：US10116821
申请日：2002-04-04
申请人： Timothy C. Dodge , Fernando Valle
发明人： Timothy C. Dodge , Fernando Valle
IPC分类号： C12P7/60 , C12P7/58
CPC分类号： C12N15/52 , C12P7/46 , C12P7/60 , C12P19/02
摘要： The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.

78. 发明授权

US06962794B2 Application of glucose transport mutants for production of aromatic pathway compounds 失效
标题翻译：葡萄糖转运突变体用于生产芳香途径化合物的应用
公开(公告)号：US06962794B2
公开(公告)日：2005-11-08
申请号：US08940692
申请日：1997-09-30
申请人： Fernando Valle , Noemi Mejia , Alan Berry
发明人： Fernando Valle , Noemi Mejia , Alan Berry
IPC分类号： C12N1/20 , C12N1/21 , C12N15/52 , C12P1/00 , C12P13/22 , C12P19/04 , C12N1/00 , C12N15/01 , C12N15/09
CPC分类号： C12P13/22 , C12N1/20 , C12N15/52 , C12P1/00 , Y10S435/813
摘要： This invention describes methods for enhancing carbon flow into a pathway of a host cell to enhance the biosynthetic production of compounds therefrom, the host cells being selected based on being phenotypically Pts−/glucose+. Such host cells are capable of transporting glucose without consuming PEP, resulting in conservation of PEP which can be re-directed into the pathway in order to enhance the production of desired compounds along the pathway. Pts−/glucose+ mutants have been shown to be advantageous for the enhanced production of the aromatic amino acids.
摘要翻译：本发明描述了用于增强进入宿主细胞途径的碳流动以增强其化合物的生物合成生产的方法，基于表型为基因表达来选择宿主细胞/ SUP>。这样的宿主细胞能够在不消耗PEP的情况下运输葡萄糖，导致PEP的保护，其可被重新引导到途径中，以便增强沿着途径产生所需化合物。已显示Pts /葡萄糖+突变体对于增强芳族氨基酸的产生是有利的。

79. 发明申请

US20050112734A1 Metabolically engineered bacterial strains having non-functional endogenous gluconate transporters 失效
标题翻译：具有非功能性内源葡萄糖酸盐转运蛋白的代谢改造的细菌菌株
公开(公告)号：US20050112734A1
公开(公告)日：2005-05-26
申请号：US10842929
申请日：2004-05-10
申请人： Timothy Dodge , Manoj Kumar , M. Rashid , Fernando Valle
发明人： Timothy Dodge , Manoj Kumar , M. Rashid , Fernando Valle
IPC分类号： C07K14/24 , C12N20060101 , C12N1/21 , C12N9/10 , C12P7/58 , C12P7/60 , C12P17/04
CPC分类号： C12P7/58 , C07K14/24 , C12P7/60
摘要： The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.
摘要翻译：本发明涉及在细菌宿主细胞中工程代谢途径，其导致用于生产抗坏血酸（ASA）中间体的增强的碳流。特别地，本发明涉及通过增加由内源性葡萄糖酸转运蛋白基因失活产生的葡萄糖酸盐的可利用性来增加细菌细胞中ASA中间体的产生。

80. 发明申请

US20050106693A1 Metabolically engineered bacterial strains having enhanced 2-keto-D-gluconate accumulation 审中-公开
标题翻译：具有增强的2-酮-D-葡萄糖酸盐积累的代谢改造的细菌菌株
公开(公告)号：US20050106693A1
公开(公告)日：2005-05-19
申请号：US10899602
申请日：2004-07-27
申请人： Timothy Dodge , M. Rashid , Fernando Valle
发明人： Timothy Dodge , M. Rashid , Fernando Valle
IPC分类号： C12N20060101 , C12N1/21 , C12N9/04 , C12N15/74 , C12P7/58 , C12P7/60 , C12P19/02
CPC分类号： C12P7/60 , C12N9/0006 , C12P19/02
摘要： The present invention relates to a method of altering bacterial host cells to accumulate 2-keto-D-gluconic acid (2-KDG) by inactivating an endogenous membrane bound 2-keto-D-gluconate dehydrogenase (2-KDGDH), which prior to inactivation catalyzed the conversion of 2-KDG to 2,5-diketogluconate (2,5-DKG).
摘要翻译：本发明涉及通过灭活内源性膜结合的2-酮-D-葡萄糖酸脱氢酶（2-KDGDH）来改变细菌宿主细胞以积累2-酮-D-葡萄糖酸（2-KDG）的方法，其在失活催化了2-KDG转化为2,5-二酮葡萄糖酸盐（2,5-DKG）。

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