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    • 75. 发明申请
    • IMMUNOASSAY FOR PLASMODIUM FALCIPARUM AND ASSAY DEVICE USED THEREFOR
    • 用于其中的PLASMODIUM FALCIPARUM和测定装置的免疫测定
    • US20090197347A1
    • 2009-08-06
    • US11571335
    • 2005-06-28
    • Mi Jin SohnSeung Bum YooYeon Chul KimJae Hoon OhEunkyung KimSeung Ho Choo
    • Mi Jin SohnSeung Bum YooYeon Chul KimJae Hoon OhEunkyung KimSeung Ho Choo
    • G01N33/569G01N33/566
    • G01N33/56905G01N33/543G01N2333/445Y02A50/58
    • Disclosed are an immunoassay of Plasmodium falciparum for determining the presence/absence of a specific and/or antibody thereof via label in conjugates bound to the specific antigen and/or antibody present in a sample, comprising immobilizing the specific antigen and antibody of Plasmodium falciparum on a solid phase, adding a sample obtained from a subject of interest to the solid phase so as to induce specific antibody-antigen reaction, adding a conjugate of the antigen and a label and a conjugate of the antibody and a label, separately prepared, so as to induce binding of at least one of the conjugates; and an assay device comprising the above-mentioned solid phase and conjugates.The present invention can effect specific detection of antigens and/or antibodies in patients with manifested malaria-symptoms as well as malaria carriers and can also be efficiently employed in samples at the early stage of malaria infection that is difficult to detect via conventional arts. Further, due to the capacity to utilize sera and blood plasma rather than whole blood, the present invention is well suited to large-scale examination such as blood screening.
    • 公开了恶性疟原虫的免疫测定法,用于通过与样品中存在的特异性抗原和/或抗体结合的缀合物中的标记物来确定其特异性和/或抗体的存在/不存在,包括将恶性疟原虫的特异性抗原和抗体固定在 固相,将从感兴趣的受试者获得的样品加入到固相中,以诱导特异性抗体 - 抗原反应,加入分别制备的抗原和抗体的标记物和缀合物和标记的缀合物, 以诱导至少一种缀合物的结合; 以及包含上述固相和缀合物的测定装置。 本发明可以在具有表现的疟疾症状的患者以及疟疾携带者中实现抗原和/或抗体的特异性检测,并且还可以有效地用于通过常规技术难以检测的疟疾感染早期的样品中。 此外,由于能够利用血清和血浆而不是全血,本发明非常适合于大规模检查,如血液筛查。