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61. 发明授权

US5916763A Promoter for VEGF receptor 失效
标题翻译： VEGF受体启动子
公开(公告)号：US5916763A
公开(公告)日：1999-06-29
申请号：US556424
申请日：1995-11-09
申请人： Lewis T. Williams , Kaoru Morishita
发明人： Lewis T. Williams , Kaoru Morishita
IPC分类号： A61K48/00 , C07K14/71 , C12N15/85 , C12Q1/68 , C07H21/04 , C12N5/00 , C12N15/00 , C12P21/06
CPC分类号： A61K48/00 , C07K14/71 , C12N15/85 , C12Q1/6897 , C12N2830/008 , C12N2830/42 , C12N2830/85
摘要： This invention provides nucleic acid sequences for a VEGF receptor promoter, particularly for the Flt-1 promoter, expression vectors and recombinant host cells containing this promoter. It also provides methods for screening for drugs that regulate the transcriptional activity of the VEGF receptor promoter. Methods for endothelial-specific gene expression and treatment of disease, particularly by inhibiting angioigenesis, using novel gene constructs containing the VEGF receptor promoter are also provided. Transgenic animals having heterologous genes linked to the VEGF receptor promoter are also provided.
摘要翻译：本发明提供VEGF受体启动子的核酸序列，特别是Flt-1启动子，表达载体和含有该启动子的重组宿主细胞。它还提供筛选调节VEGF受体启动子的转录活性的药物的方法。还提供了使用含有VEGF受体启动子的新基因构建体的内皮特异性基因表达和疾病治疗方法，特别是通过抑制血管发生的方法。还提供了具有与VEGF受体启动子连接的异源基因的转基因动物。

62. 发明授权

US5817911A Transgenic mice expressing alveolar MCP-1 失效
标题翻译：表达肺泡MCP-1的转基因小鼠
公开(公告)号：US5817911A
公开(公告)日：1998-10-06
申请号：US420001
申请日：1995-04-07
申请人： Lewis T. Williams , Michael Dee Gunn
发明人： Lewis T. Williams , Michael Dee Gunn
IPC分类号： C07K14/52 , C12N15/85 , C12N15/63 , A61K48/00 , C12N15/00
CPC分类号： C12N15/8509 , A01K67/0278 , C07K14/523 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0368
摘要： A transgenic, non-human animal is provided which overexpresses a gene responsible for the accumulation of monocytes and leukocytes. The animal is preferably a mammal and more preferably a mouse, rat or guinea pig. The transgenic animal is created by artificially inserting a transgene into a fertilized egg of the animal which egg is then inserted within a pseudo pregnant female where it is allowed to grow. The transgene preferably expresses human Monocyte Chemoattractant Protein-1 (MCP-1) and more preferably overexpresses MCP-1 in type II pulmonary epithelial cells. The invention includes DNA constructs and vectors containing the constructs with a particularly preferred vector being SPC-MCP-1. The plasmid SPC-MCP-1 includes a promoter operatively linked to a human MCP-1 coding sequence. The transgenic animal provides a useful animal model for testing drugs for their efficacy with respect to the treatment of diseases and conditions which result in an acceptable high accumulation of monocytes and/or lymphocytes.
摘要翻译：提供了转基因非人动物，其过表达负责单核细胞和白细胞积累的基因。动物优选为哺乳动物，更优选为小鼠，大鼠或豚鼠。转基因动物是通过人工将转基因插入动物的受精卵而产生的，然后将卵插入允许其生长的假怀孕雌性中。转基因优选表达人单核细胞趋化蛋白-1（MCP-1），更优选在II型肺上皮细胞中过表达MCP-1。本发明包括含有构建体的DNA构建体和载体，特别优选的载体是SPC-MCP-1。质粒SPC-MCP-1包括可操作地连接人MCP-1编码序列的启动子。转基因动物提供了一种有用的动物模型，用于测试药物在治疗导致单核细胞和/或淋巴细胞可接受的高度积累的疾病和病症方面的功效。

63. 发明授权

US5744313A Assay employing novel protein domain which binds tyrosine phosphorylated proteins 失效
标题翻译：测定使用结合酪氨酸磷酸化蛋白质的新蛋白质结构域
公开(公告)号：US5744313A
公开(公告)日：1998-04-28
申请号：US353550
申请日：1994-12-09
申请人： Lewis T. Williams , William Michael Kavanaugh
发明人： Lewis T. Williams , William Michael Kavanaugh
IPC分类号： G01N33/566 , A61K38/00 , A61K48/00 , A61P9/10 , A61P17/00 , A61P29/00 , A61P35/00 , A61P43/00 , C07K14/47 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/12 , C12P21/02 , C12Q1/68 , G01N33/53
CPC分类号： C07K14/47 , A61K38/00
摘要： The present invention generally relates to novel protein domains which bind to tyrosine-phosphorylated proteins. More particularly, the present invention provides non-SH2 protein domains that bind phosphotyrosine. The present invention also provides various composite polypeptides comprising these domains, such that these composite polypeptides retain the ability to bind tyrosine phosphorylated proteins. It also provides nucleic acids encoding these polypeptides. Typically, these nucleic acids may also comprise a promoter for expression, and a segment encoding fusion peptides on the amino or carboxy terminus of the expressed composite protein. Also included within the present invention are methods of preparing these polypeptides and cells capable of expressing them. Also provided are methods of using these polypeptides in research, diagnostic and therapeutic applications.
摘要翻译：本发明一般涉及结合酪氨酸磷酸化蛋白质的新型蛋白质结构域。更具体地，本发明提供了结合磷酸酪氨酸的非SH2蛋白结构域。本发明还提供了包含这些结构域的各种复合多肽，使得这些复合多肽保留结合酪氨酸磷酸化蛋白的能力。它还提供编码这些多肽的核酸。通常，这些核酸还可以包含用于表达的启动子，以及在表达的复合蛋白的氨基或羧基末端编码融合肽的区段。本发明还包括制备这些多肽和能够表达它们的细胞的方法。还提供了在研究，诊断和治疗应用中使用这些多肽的方法。

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