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51. 发明授权

US4278653A Methods and kits for double antibody immunoassay providing a colored pellet for easy visualization 失效
标题翻译：用于双抗体免疫测定的方法和试剂盒，提供有色颗粒以便于观察
公开(公告)号：US4278653A
公开(公告)日：1981-07-14
申请号：US8267
申请日：1979-02-01
申请人： S. Richard Harris , Darlene K. Bonci
发明人： S. Richard Harris , Darlene K. Bonci
IPC分类号： G01N33/532 , G01N33/56 , G01N33/78
CPC分类号： G01N33/532 , Y10S436/805 , Y10S436/808 , Y10S436/815 , Y10S436/817
摘要： Methods and kits for performing a double antibody immunoassay are described. The immunoassay comprises:reacting an antigen and a labelled version of said antigen with a first antibody against said antigen and said first antibody with a second antibody against said first antibody to precipitate a complex of said antigen, said first antibody and said second antibody, andprecipitating a colored protein to form a precipitate comprised of said complex and colored protein.In a preferred embodiment, the first and second antibodies can be prereacted to simplify the assay procedure.
摘要翻译：描述了用于进行双重抗体免疫测定的方法和试剂盒。所述免疫测定包括：使抗原和所述抗原的标记形式与针对所述抗原和所述第一抗体的第一抗体与针对所述第一抗体的第二抗体反应以沉淀所述抗原，所述第一抗体和所述第二抗体的复合物，以及沉淀有色蛋白质以形成由所述复合物和有色蛋白质组成的沉淀物。在优选的实施方案中，可以预先反应第一和第二抗体以简化测定程序。

52. 发明授权

US4272505A Competitive binding analysis by fluorescence 失效
标题翻译：通过荧光进行竞争结合分析
公开(公告)号：US4272505A
公开(公告)日：1981-06-09
申请号：US830009
申请日：1977-09-01
申请人： David S. Smith
发明人： David S. Smith
IPC分类号： G01N33/53 , G01N33/542 , G01N33/78 , G01N21/64 , G01N33/54 , G01N33/58
CPC分类号： G01N33/78 , Y10S436/80 , Y10S436/805
摘要： Thyroid hormones such as thyroxine and triiodothyronine are assayed in biological fluid samples by a competitive binding analysis in which a fluorescent label is used. Upon formation of complexes, the fluorescence of the labelled compound is released, and the hormone can be assayed by measuring the fluorescence of the mixture and comparing the result with standard data. Fluorescein is the preferred label. The process can be operated in a continuous flow manner.
摘要翻译：通过竞争性结合分析在生物液体样品中测定甲状腺素和三碘甲状腺原氨酸的甲状腺激素，其中使用荧光标记。复合物形成后，释放标记化合物的荧光，通过测量混合物的荧光并将结果与标准数据进行比较，可以测定激素。荧光素是首选标签。该过程可以以连续流动方式操作。

53. 发明授权

US4251360A Method and apparatus for the detection of a specific binding protein 失效
标题翻译：用于检测特异性结合蛋白的方法和装置
公开(公告)号：US4251360A
公开(公告)日：1981-02-17
申请号：US53887
申请日：1979-07-02
申请人： David J. Goldie , Adel Abbas A. Ismail , Peter M. West
发明人： David J. Goldie , Adel Abbas A. Ismail , Peter M. West
IPC分类号： G01N27/40 , G01N33/543 , G01N33/78 , B01D13/00
CPC分类号： G01N33/78 , G01N33/54366 , Y10S436/80 , Y10S436/804 , Y10S436/808 , Y10S436/826
摘要： The invention provides a method for detecting and determining one component of the reaction between a specific binding protein, e.g., an antibody, and the corresponding bindable substance, e.g., an antigen, by utilizing the affinity of these components for binding one another, in an automated system utilizing a continuously flowing stream of liquid. The method is carried out by introducing successive mixtures of the sample specimen with a set amount of one component of the reaction in tagged form and another, carrier-bound, component, into the liquid stream so that the individual mixtures remain separate, incubating the mixtures and continuously introducing same into a separating system in which at least a portion of the liquid phase is separated from the solid phase, and measuring the amount of the tagged component in one of the separated phases as a measure of the unknown component to be detected. For instance, an unknown antigen in a serum sample is contacted with a solid carrier bound antibody, the mixture incubated, the antigen now bound to the solid phase antibody, removed from the other mixture components and reacted with an additional marked antibody acting against the antigen, to form a sandwich containing solid-bound antibody/antigen/marked antibody, and then separating the solid phase from the liquid phase containing the remaining free-tagged antibody and determining the tagged antibody in either fraction as a measure of the serum antigen initially present.
摘要翻译：本发明提供了一种用于通过利用这些成分彼此结合的亲和力来检测和确定特异性结合蛋白（例如抗体）和相应的可结合物质（例如抗原）之间的反应的一种成分的方法，自动化系统利用连续流动的液体流。该方法通过将样品样品的连续混合物与设定量的标记形式的一种反应组分和另一种载体结合的组分引入液体流中，使得各个混合物保持分离，将混合物并将其连续地引入分离系统中，其中液相的至少一部分与固相分离，并测量一个分离相中的标记组分的量作为待检测未知组分的量度。例如，将血清样品中的未知抗原与固体载体结合的抗体接触，将混合物孵育，现在与固相抗体结合的抗原从其它混合物组分中除去，并与另外标记的抗体作用于抗原，以形成含有固体结合抗体/抗原/标记抗体的夹心，然后从含有剩余自由标记抗体的液相中分离固相，并测定任一级分中的标记抗体作为最初存在的血清抗原的量度。

54. 发明授权

US4250253A Composition adapted for the determination of tri-iodo thyronine and diagnosis method employing same 失效
标题翻译：用于测定三碘甲状腺素的组合物及其使用的诊断方法
公开(公告)号：US4250253A
公开(公告)日：1981-02-10
申请号：US884811
申请日：1978-03-09
申请人： Pierluigi Renzi , Vittorio Baroncelli
发明人： Pierluigi Renzi , Vittorio Baroncelli
IPC分类号： G01N33/53 , A61B10/00 , A61K39/44 , C12Q1/26 , G01N33/543 , G01N33/74 , G01N33/78 , C12Q1/66 , C12N9/96
CPC分类号： G01N33/78 , A61K39/44
摘要： A composition for diagnostic use is disclosed, more particularly for the dosage of tri-iodo thyronine in human blood serum: the subject composition comprises tri-iode thyronine as such and an enzyme which is covalently bonded to tri-iodo thyronine. A diagnostic method is also disclosed, which is intended for using the above specified composition in diagnostic runs. The method, briefly resumed, consists removing the tri-iodo thyronine of the serum sample being assayed by means of an insolubilized anti-T.sub.3 antibody and treating the residue with the sensibilized enzyme which is nothing else than the covalent formed by tri-iodo thyronine and an appropriate enzyme, such as carbonic anhydrase.
摘要翻译：公开了一种用于诊断用途的组合物，更具体地涉及人血清中三碘甲状腺素的剂量：主题组合物包含三碘代甲状腺原样和与三碘甲状腺原体共价结合的酶。还公开了一种诊断方法，其旨在在诊断运行中使用上述指定的组合物。该方法简单地恢复，通过不溶解的抗-T3抗体去除正在测定的血清样品的三碘甲状腺原理，用感应酶处理残留物，这不仅仅是由三碘甲状腺素形成的共价和合适的酶，如碳酸酐酶。

55. 发明授权

US4238471A Assay for thyroid hormone binding capacity 失效
标题翻译：测定甲状腺激素结合能力
公开(公告)号：US4238471A
公开(公告)日：1980-12-09
申请号：US923712
申请日：1978-07-12
申请人： Max G. Reese , LaVell R. Johnson
发明人： Max G. Reese , LaVell R. Johnson
IPC分类号： G01N33/53 , G01N33/543 , G01N33/74 , G01N33/78 , G01N33/16 , B01J1/22 , G21H5/02
CPC分类号： G01N33/78 , Y10S436/808
摘要： Automated assay for qualitative determination of thyroid hormone binding capacity of a serum sample in which serum and T.sub.3 tracer are caused to flow through a chamber containing a T.sub.3 binder supported on a solid support. The amount of T.sub.3 tracer which passes through the chamber, and the amount of T.sub.3 tracer bound to the binder in the chamber are determined to thereby determine the percent of bound T.sub.3 tracer. Such percent is then compared with the percent of T.sub.3 tracer bound in a referenced sample to qualitatively determine the T.sub.3 binding capacity of the serum sample.
摘要翻译：用于定性测定血清样品的甲状腺激素结合能力的自动测定法，其中使血清和T3示踪剂流过包含支持在固体支持物上的T3粘合剂的腔室。确定穿过室的T3示踪剂的量和与室中的粘合剂结合的T3示踪剂的量，从而确定结合的T3示踪剂的百分比。然后将这样的百分比与在参考样品中结合的T3示踪剂的百分比进行比较，以定性确定血清样品的T3结合能力。

56. 发明授权

US4225784A Covalently bound biological substances to plastic materials and use in radioassay 失效
标题翻译：将生物物质共价结合到塑料材料中并用于放射性测定
公开(公告)号：US4225784A
公开(公告)日：1980-09-30
申请号：US696959
申请日：1976-06-17
申请人： M. James Barrett
发明人： M. James Barrett
IPC分类号： G01N33/53 , G01N33/543 , G01N33/545 , G01N33/78 , G01N33/82 , G01T1/161
CPC分类号： G01N33/78 , G01N33/545 , G01N33/82 , Y10S436/808 , Y10S436/81
摘要： Covalently bound biological substances to plastic materials whose inside surfaces have been coated with glutaraldehyde, with or without prior treatment with an aliphatic amine or diamine, useful in radioimmunoassay procedures.
摘要翻译：将共价结合的生物物质与其内表面已经用戊二醛包被的塑料材料共混，可用或不用用脂肪胺或二胺预处理，可用于放射免疫测定程序。

57. 发明授权

US4155711A Method and apparatus for determining thyroid function of multiple samples 失效
标题翻译：确定多个样本甲状腺功能的方法和装置
公开(公告)号：US4155711A
公开(公告)日：1979-05-22
申请号：US589688
申请日：1975-06-24
申请人： Michael Zelagin , Raymond A. Smutko
发明人： Michael Zelagin , Raymond A. Smutko
IPC分类号： G01N1/40 , G01N33/483 , G01N33/78 , G01N33/16
CPC分类号： G01N33/78 , G01N1/405 , Y10S436/804 , Y10S436/808 , Y10S436/81 , Y10S436/817
摘要： A method and apparatus for measuring thyroid function by means of measuring the up-take of a radioactive tracer on a resin involves causing the mixing of resin with tracer for predetermined periods and preferably by a means which causes agitation of the resin in the tracer-containing sample to be tested. Also disclosed is an apparatus useful for handling multiple samples to be tested.
摘要翻译：用于通过测量放射性示踪剂在树脂上的测量来测量甲状腺功能的方法和装置包括使树脂与示踪剂混合预定的时间，优选地通过引起含有示踪剂的树脂中的树脂搅拌的方式待测样品。还公开了一种可用于处理要测试的多个样品的装置。

58. 发明授权

US4069105A Lidocaine antigens and antibodies 失效
标题翻译：利多卡因抗原和抗体
公开(公告)号：US4069105A
公开(公告)日：1978-01-17
申请号：US775658
申请日：1977-03-03
申请人： Prithipal Singh
发明人： Prithipal Singh
IPC分类号： C08G69/00 , C07K14/00 , C07K14/76 , C07K16/00 , C07K16/44 , C08G69/10 , C08G69/48 , C08H1/00 , C12Q1/00 , G01N33/15 , G01N33/53 , G01N33/535 , G01N33/78 , C07G7/00
CPC分类号： G01N33/535 , C07K16/44 , Y10S435/961 , Y10S435/964 , Y10S436/816 , Y10S530/806
摘要： Derivatives of anesthetics involving anilides, lidocaine being illustrative, are provided, having an annular amino group. A difunctional linking group is provided, which provides a link to the annular amino group and an antigen, with the resulting conjugate being employed for the preparation of antibodies. The antibodies find particular use in competitive protein binding assays. Conjugates to enzymes are prepared which find particular use in homogeneous enzyme immunoassays.
摘要翻译：提供涉及苯胺的麻醉剂的衍生物，作为说明性的利多卡因，具有环形氨基。提供了双官能连接基团，其提供了与环状氨基和抗原的连接，所得到的缀合物用于制备抗体。抗体在竞争性蛋白质结合测定中特别有用。制备与酶的结合物，其在均相酶免疫测定中特别有用。

59. 发明授权

US4052504A Assay for thyroxine binding globulin 失效
标题翻译：甲硫氨酸结合GLOBULIN的测定
公开(公告)号：US4052504A
公开(公告)日：1977-10-04
申请号：US701191
申请日：1976-06-30
申请人： William Hertl , Gerald Odstrchel
发明人： William Hertl , Gerald Odstrchel
IPC分类号： G01N33/78 , G01N33/00 , G01N33/16
CPC分类号： G01N33/78 , Y10S436/804
摘要： Method of determining the concentration of thyroxine binding globulin (TBG) in a fluid sample. The method comprises the steps of adding to the sample an excess amount of thyroxine (T.sub.4) and then analyzing the sample for T.sub.4 via immunoassay technique in the presence and absence of a blocking agent to establish a binding differential. The differential is then correlated with a standard curve which relates known TBG concentrations to binding differentials obtained in a similar manner.

60. 发明授权

US4046870A Assay for free thyroid hormones 失效
标题翻译：测定游离甲状腺激素
公开(公告)号：US4046870A
公开(公告)日：1977-09-06
申请号：US701192
申请日：1976-06-30
申请人： William Hertl , Gerald Odstrchel
发明人： William Hertl , Gerald Odstrchel
IPC分类号： G01N33/53 , G01N33/74 , G01N33/78 , G01N33/00 , G01N33/16
CPC分类号： G01N33/78
摘要： Method for determining the concentration of free (unbound) thyroid hormones (T.sub.3 or T.sub.4) in a fluid sample. The method comprises the steps of performing an immunoassay for total T.sub.3 or total T.sub.4 in the presence and absence of a blocking agent to establish a binding differential and then correlating that differential with a standard curve which relates known free thyroid hormone concentrations with binding differentials.
摘要翻译：确定流体样品中游离（未结合）甲状腺激素（T3或T4）浓度的方法。该方法包括以下步骤：在存在和不存在阻断剂的情况下对总T3或总T4进行免疫测定以建立结合差异，然后将该差异与将已知的游离甲状腺激素浓度与结合差异相关联的标准曲线相关联。

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