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51. 发明授权

US09175330B2 Method for screening and quantifying isoprene biosynthesis enzyme activity 有权
标题翻译：筛选和定量异戊二烯生物合成酶活性的方法
公开(公告)号：US09175330B2
公开(公告)日：2015-11-03
申请号：US13771952
申请日：2013-02-20
申请人： Korea Research Institute of Bioscience and Biotechnology
发明人： Seung-Goo Lee , Seo Hyeon Kim , Jongsik Gam , Eugene Rha , Haseong Kim , Su-Lim Choi , EuiSung Choi
IPC分类号： C12Q1/02 , C12Q1/25 , C40B30/08 , C12Q1/68 , C12N15/10
CPC分类号： C12Q1/25 , C12N15/1086 , C12Q1/02 , C12Q1/68 , C12Q1/6897 , C40B30/08
摘要： A method of performing high-throughput screening of various enzymatic activities with high sensitivity using artificial genetic circuits is provided. Particularly, the invention is screening and quantifying the activity of isoprene biosynthesis enzymes using an artificial genetic circuit capable of sensing isoprene. The artificial genetic circuit comprises an isoprene-sensing transcriptional regulator which recognizes isoprene, at least one reporter gene, a isoprene-sensing transcriptional regulator binding region and promoters for genes encoding isoprene-sensing transcriptional regulators and reporter proteins.The artificial genetic circuit detects isoprene liberated from many enzymatic reactions and measures the activity of reporter genes. This system is widely applicable for high throughput and quantitative screening of isoprene biosynthesis enzymes and MEP/MVA pathway enzymes.Therefore, the invention can be advantageously used in the protein engineering technology for enzyme modification. Particularly, it can provide a quantitative investigation of enzymatic activity, and thus can be applied to molecular evolution technology.
摘要翻译：提供了使用人造遗传电路以高灵敏度进行各种酶活性的高通量筛选的方法。特别地，本发明使用能够感测异戊二烯的人造遗传电路来筛选和定量异戊二烯生物合成酶的活性。人造遗传电路包括识别异戊二烯的异戊二烯感测转录调节子，至少一个报告基因，异戊二烯感测转录调节子结合区和编码异戊二烯感测转录调节子和报道蛋白的基因的启动子。人工遗传回路检测许多酶反应释放的异戊二烯，并测量报告基因的活性。该系统广泛应用于异戊二烯生物合成酶和MEP / MVA途径酶的高通量和定量筛选。因此，本发明可有利地用于酶修饰的蛋白质工程技术中。特别是可以提供酶活性的定量研究，因此可以应用于分子进化技术。

52. 发明授权

US08932994B2 Method for retaining even coverage of short insert libraries 有权
标题翻译：保留短插入库的均匀方法
公开(公告)号：US08932994B2
公开(公告)日：2015-01-13
申请号：US11895294
申请日：2007-08-23
申请人： Niall Anthony Gormley , Melanie Anne Smith
发明人： Niall Anthony Gormley , Melanie Anne Smith
IPC分类号： C40B50/00 , C12Q1/68 , C12Q1/25
CPC分类号： C12Q1/6855 , C12Q2533/101
摘要： The invention relates to a method of preparing a library of template polynucleotides with uniform sequence representation and to use of a library of templates prepared using this method for solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends, which contains even representation of all the fragments present in a starting sample of nucleic acid before fragmentation. The invention is especially applicable to the preparation of short insert libraries, where the sample fragments are less than 150 base pairs in length.
摘要翻译：本发明涉及一种制备具有均匀序列表示的模板多核苷酸文库的方法，以及使用该方法制备的模板文库用于固相核酸扩增。特别地，本发明涉及一种制备模板多核苷酸文库的方法，该文库在其5'末端和3'末端具有共同序列，其包含在分裂前核酸起始样品中存在的所有片段的均匀表示。本发明特别适用于制备短插入文库，其中样品片段长度小于150个碱基对。

53. 发明授权

US08791258B2 Pro-fluorescent probes 有权
标题翻译：荧光探针
公开(公告)号：US08791258B2
公开(公告)日：2014-07-29
申请号：US12997234
申请日：2009-06-08
申请人： Christopher J. Chang
发明人： Christopher J. Chang
IPC分类号： C07F5/02 , G01N21/76 , C40B40/04 , C07K2/00 , C07K16/00 , C07H23/00 , G01N33/22 , C12Q1/25
CPC分类号： C07F5/025 , C12Q1/28
摘要： The present invention provides a novel class of pro-fluorescent probes for reactive oxygen species (ROS). One exemplary probe is mitochondria peroxy yellow 1 (MitoPY1), a new type of flurophore for imaging mitochondrial H2O2 in living cells with ROS and spatial specificity. The invention also provides methods of using pro-fluorescent probes to detect analytes. One exemplary method comprises using a pro-fluorescent probe of the invention to detect an explosive.
摘要翻译：本发明提供了一类新型的用于活性氧（ROS）的前荧光探针。一个典型的探针是线粒体过氧黄1（MitoPY1），一种用于在具有ROS和空间特异性的活细胞中线粒体H2O2成像的新型荧光。本发明还提供了使用前荧光探针来检测分析物的方法。一个示例性方法包括使用本发明的荧光探针来检测爆炸物。

54. 发明授权

US08728800B2 Assay method 有权
标题翻译：测定方法
公开(公告)号：US08728800B2
公开(公告)日：2014-05-20
申请号：US11664540
申请日：2006-02-23
申请人： Frank Frantzen , Arne Ludvig Faaren , Arne Kristian Nordhei
发明人： Frank Frantzen , Arne Ludvig Faaren , Arne Kristian Nordhei
IPC分类号： G01N33/53 , G01N33/533 , C12Q1/25
CPC分类号： G01N33/6815 , B01L3/021 , B01L3/502 , B01L2200/10 , B01L2300/0681 , G01N35/026 , G01N35/1016 , G01N35/1079 , G01N2035/1032 , G01N2035/1039
摘要： The invention provides a cassette-based automated assay for homocysteine.
摘要翻译：本发明提供了一种基于盒式的同型半胱氨酸自动测定法。

55. 发明授权

US08691152B2 System and method for analyte measurement 有权
标题翻译：用于分析物测量的系统和方法
公开(公告)号：US08691152B2
公开(公告)日：2014-04-08
申请号：US13364365
申请日：2012-02-02
申请人： David W. Burke , Lance S. Kuhn , Terry A. Beaty , Vladimir Svetnik
发明人： David W. Burke , Lance S. Kuhn , Terry A. Beaty , Vladimir Svetnik
IPC分类号： G01N27/04 , G01N27/327 , G01N33/50 , C12Q1/25
CPC分类号： G01N27/3274 , Y10T436/11 , Y10T436/112499 , Y10T436/115831 , Y10T436/12 , Y10T436/142222 , Y10T436/143333 , Y10T436/144444 , Y10T436/203332
摘要： A method of measuring an analyte in a biological fluid comprises applying an excitation signal having a DC component and an AC component. The AC and DC responses are measured; a corrected DC response is determined using the AC response; and a concentration of the analyte is determined based upon the corrected DC response. Other methods and devices are disclosed.
摘要翻译：测量生物流体中的分析物的方法包括施加具有DC分量和AC分量的激发信号。测量AC和DC响应; 使用AC响应确定校正的DC响应; 并且基于校正的DC响应确定分析物的浓度。公开了其它方法和装置。

56. 发明授权

US08652771B2 Measurement of succinate in urine samples as a biomarker of kidney damage in diabetic subjects 有权
标题翻译：测量尿液样品中的琥珀酸盐作为糖尿病受试者肾脏损伤的生物标志物
公开(公告)号：US08652771B2
公开(公告)日：2014-02-18
申请号：US12473135
申请日：2009-05-27
申请人： Janos Peti-Peterdi
发明人： Janos Peti-Peterdi
IPC分类号： C12Q1/25
CPC分类号： G01N33/70 , G01N2800/347
摘要： The present invention relates in general to the discovery of urinary succinate as a novel biomarker of kidney disease. More specifically, the invention provides for the measurement of succinate in urine samples that has great potential for the easy and early diagnosis of kidney damage and would allow early prediction of kidney disease and therapeutic intervention.
摘要翻译：本发明一般涉及尿琥珀酸盐作为肾脏疾病的新型生物标志物的发现。更具体地，本发明提供了尿样中琥珀酸盐的测量，其对于肾损伤的容易和早期诊断具有很大的潜力，并且将允许早期预测肾脏疾病和治疗干预。

57. 发明申请

US20140033370A1 VISUAL SCREENING FOR ENZYMES ON THE PHENYLPROPANOID PATHWAY 审中-公开
标题翻译：在苯丙氨酸途径上的酶的视觉筛选
公开(公告)号：US20140033370A1
公开(公告)日：2014-01-30
申请号：US13992742
申请日：2011-12-08
申请人： Hagai Abeliovich , Moti Alberstein
发明人： Hagai Abeliovich , Moti Alberstein
IPC分类号： C12Q1/68 , C12N9/88 , C12Q1/25 , C12N9/00 , C12Q1/527 , C12Q1/26
CPC分类号： C12Q1/6897 , C12N9/0073 , C12N9/1037 , C12N9/88 , C12N9/93 , C12N15/8243 , C12N15/825 , C12Q1/25 , C12Q1/26 , C12Q1/527 , C12Y114/13011 , C12Y203/01074 , C12Y403/01005 , C12Y602/01012
摘要： The invention relates to a method to screen for a nucleic acid molecule encoding a polypeptide involved in the synthesis of naringenin chalcone which demonstrates altered expression, activity or substrate specificity. The invention further relates to nucleic acid molecules identified by said screen and to transgenic plants modified to express said nucleic acid molecule.
摘要翻译：本发明涉及一种筛选编码参与柚皮素查耳酮合成的多肽的核酸分子的方法，其表现出改变的表达，活性或底物特异性。本发明还涉及由所述筛选鉴定的核酸分子和修饰以表达所述核酸分子的转基因植物。

58. 发明授权

US08603308B2 Electrical patterns for biosensor and method of making 有权
标题翻译：生物传感器的电气图案和制作方法
公开(公告)号：US08603308B2
公开(公告)日：2013-12-10
申请号：US12770507
申请日：2010-04-29
申请人： Raghbir Singh Bhullar , Mike Celenatano , Said K. El-Rahaiby
发明人： Raghbir Singh Bhullar , Mike Celenatano , Said K. El-Rahaiby
IPC分类号： G01N27/327 , C12Q1/25 , H05K3/00
CPC分类号： G01N27/3277 , G01N27/3272 , G01N33/5438
摘要： The present invention provides an inventive biosensor that includes multiple regions in which the electrical pattern is formed from different electrically conductive materials. The present invention also provides an inventive method for mass producing biosensors as just described. In one embodiment of this method, first and second different electrically conductive materials are deposited side by side on a portion of an electrically insulating base material, and a plurality of electrical patterns is formed on the portion of the base material. Each electrical pattern includes a first region formed from the first electrically conductive material electrically connected to a second region formed from the second electrically conductive material. The electrically conductive materials can be deposited as layers on the base material and portions of the layers can be removed to form the electrical patterns, or, the electrical patterns can be formed by transferring the conductive material in the shape of the electrical pattern directly to the base material, such as by a laser direct transfer technique.
摘要翻译：本发明提供了本发明的生物传感器，其包括多个区域，其中电图案由不同的导电材料形成。本发明还提供了如上所述的用于批量生产生物传感器的发明方法。在该方法的一个实施例中，第一和第二不同的导电材料并排地沉积在电绝缘基材的一部分上，并且在基材的部分上形成多个电图案。每个电图案包括由第一导电材料形成的第一区域，电连接到由第二导电材料形成的第二区域。导电材料可以作为层沉积在基底材料上，并且可以去除这些层的部分以形成电图案，或者可以通过将电图案形状的导电材料直接转移到基材，例如通过激光直接转印技术。

59. 发明申请

US20130280731A1 RATIONAL ENZYME MINING 有权
标题翻译：合理的采食
公开(公告)号：US20130280731A1
公开(公告)日：2013-10-24
申请号：US13989513
申请日：2011-11-29
申请人： Martin Karlsson , Bengt-Harald Jonsson , Uno Carlsson
发明人： Martin Karlsson , Bengt-Harald Jonsson , Uno Carlsson
IPC分类号： C12Q1/25
CPC分类号： C12Q1/25 , C12N1/36 , C12Q1/02
摘要： A method for rational mining for induced enzymes in microbial communities is described. The method is characterized in that a community of microorganisms is provided and that the microbial populations of the community are cultivated in a container under conditions of choice, where the microorganisms are given a defined culturing medium, to eliminate matter deriving from the natural habitat and to allow the microbial community to reach a metabolic steady-state. The method is further characterized in that at least one fraction of microorganisms is taken from the container and transferred into at least two separate containers, where at least one of the fractions of microorganisms is provided with a defined medium which includes an inducing substance and/or a substance against which enzyme/enzymes is/are desired, to induce regulation of expression of the desired enzyme/enzymes, and at least one fraction is provided with a defined medium without said inducing substance and without said substance against which enzyme/enzymes is/are desired, for the purpose of comparison, and the fractions are maintained and/or cultivated. Samples of the two fractions are then withdrawn and analyzed for identification of the induced enzyme/enzymes.
摘要翻译：描述了微生物群落中诱导酶合理开采的方法。该方法的特征在于提供微生物群落，并且在选择的条件下将容器中的微生物种群培养在容器中，其中微生物被赋予限定的培养基，以消除源于自然栖息地的物质，并且使微生物群落达到代谢稳态。该方法的特征还在于至少一部分微生物从容器中取出并转移到至少两个分开的容器中，其中至少一个微生物部分具有限定的培养基，其包括诱导物质和/或提供酶/酶所需的物质以诱导所需酶/酶的表达的调节，并且至少一个级分具有没有所述诱导物质的限定的培养基，并且没有所述酶/酶是/ 为了比较的目的，并且维持和/或培养级分。然后取出两个级分的样品并分析以鉴定诱导的酶/酶。

60. 发明申请

US20130196849A1 Substituted Cellulose Acetates and Uses Thereof 有权
标题翻译：取代的醋酸纤维素及其用途
公开(公告)号：US20130196849A1
公开(公告)日：2013-08-01
申请号：US13359614
申请日：2012-01-27
申请人： Michael T. Combs , Thomas S. Garrett
发明人： Michael T. Combs , Thomas S. Garrett
IPC分类号： A61K9/00 , A61K31/137 , A61K31/485 , A61K31/4355 , A61K31/045 , A61K33/02 , G01N33/53 , C12Q1/25 , A61P29/00 , A61P9/06 , A61P11/06 , A61P31/04 , A61P31/12 , A61P7/02 , A61P25/28 , A61P25/24 , A61P3/10 , A61P9/12 , A61P33/06 , A61P25/06 , A61P35/00 , A61P37/06 , A61P33/02 , A61P5/16 , A61P11/14 , A61P25/22 , A61P25/20 , A61P9/04 , A61K31/56 , A61P7/10 , A61P1/00 , A61P25/16 , A61P17/12 , A61P3/00 , A61P21/02 , A61P31/10 , A61P15/10 , A61P23/00 , A61P11/10 , A61P11/02 , A61F2/82 , A01C1/06
CPC分类号： A61L31/10 , A61K9/5042 , A61K31/137 , A61K31/216 , A61K31/4741 , A61K31/485 , A61K31/5415 , A61K33/02 , A61K47/38 , A61L2400/16 , C08B3/06 , C08B7/00 , C08L1/20 , C09D101/20 , C09J101/20 , C09K8/035 , G01N5/02 , G01N19/04 , G01N2203/0025 , Y02A50/411 , C08L1/12
摘要： The present invention relates to substituted cellulose acetates and methods of use thereof. One embodiment of the present invention provides a coating having a substituted cellulose acetate having a polar substituent that has an oxygen atom covalently bonded to a nonmetal selected from the group of sulfur, phosphorus, and boron; wherein the nonmetal is present in at least about 0.01% by weight of the substituted cellulose acetate.
摘要翻译：本发明涉及取代的乙酸纤维素及其使用方法。本发明的一个实施方案提供一种具有取代的乙酸纤维素的涂层，其具有极性取代基，其具有与选自硫，磷和硼的非金属共价键合的氧原子; 其中所述非金属以至少约0.01重量％的所述取代的乙酸纤维素存在。

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