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51. 发明申请

US20100003724A1 CHEMICALLY MODIFIED NUCLEOSIDE 5'-TRIPHOSPHATES FOR THERMALLY INITIATED AMPLIFICATION OF NUCLEIC ACID 有权
标题翻译：化学改性核壳素5'-磷酸酯，用于热启动核酸扩增
公开(公告)号：US20100003724A1
公开(公告)日：2010-01-07
申请号：US12470449
申请日：2009-05-21
申请人： Alexandre Lebedev , Inna Koukhareva
发明人： Alexandre Lebedev , Inna Koukhareva
IPC分类号： C12P19/34
CPC分类号： C07H19/00 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q2525/101 , C12Q2525/186 , C12Q2545/101
摘要： Provided herein are methods and compositions for nucleic acid replication. These methods involve the use of 3′-substituted nucleoside 5′-triphosphates or 3′-substituted terminated primers in nucleic acid replication reactions. In certain aspects, the methods are accomplished by use of 3′-substituted NTPs and/or 3′-substituted terminated primers which provide utility in nucleic acid replication. In preferred embodiments, the NTPs and/or primers are substituted at the 3′-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.
摘要翻译：本文提供了用于核酸复制的方法和组合物。这些方法涉及在核酸复制反应中使用3'-取代核苷5'-三磷酸或3'-取代的终止引物。在某些方面，所述方法通过使用在核酸复制中提供效用的3'-取代的NTP和/或3'-取代的封端引物来实现。在优选的实施方案中，NTP和/或引物在3'-位置被特定的热不稳定化学基团例如醚，酯或碳酸酯代替。

52. 发明授权

US07622279B2 Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry 有权
标题翻译：用于通过位点特异性偶联化学构建的芯片上的DNA测序的可透光荧光核苷酸
公开(公告)号：US07622279B2
公开(公告)日：2009-11-24
申请号：US10591520
申请日：2005-03-03
申请人： Jingyue Ju
发明人： Jingyue Ju
IPC分类号： C12P19/34 , C12Q1/68 , C07G3/00 , C07H19/04 , C07H21/00 , C07H21/02
CPC分类号： C12Q1/6874 , C12Q2525/101 , C12Q2523/319
摘要： This invention provides a method for determining the sequence of a DNA or an RNA, wherein (i) about 1000 or fewer copies of the DNA or RNA are bound to a solid substrate via 1,3-dipolar azide-alkyne cycloaddition chemistry and (ii) each copy of the DNA or RNA comprises a self-priming moiety.
摘要翻译：本发明提供了一种用于确定DNA或RNA序列的方法，其中（i）约1000个或更少拷贝的DNA或RNA通过1,3-偶极叠氮化物 - 炔环化学化学结合到固体底物上，和（ii））DNA或RNA的每个拷贝包含自引发部分。

53. 发明授权

US07541147B2 Materials and methods for detection of nucleic acids 有权
公开(公告)号：US07541147B2
公开(公告)日：2009-06-02
申请号：US11546631
申请日：2006-10-12
申请人： David J. Marshall , James R. Prudent , Christopher B. Sherrill , Gideon Shapiro , Simona Jurczyk , Jerod L. Ptacin
发明人： David J. Marshall , James R. Prudent , Christopher B. Sherrill , Gideon Shapiro , Simona Jurczyk , Jerod L. Ptacin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6834 , C12Q1/6858 , C12Q2600/156 , C12Q2565/1015 , C12Q2525/101 , C12Q2525/161
摘要： Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.

54. 发明授权

US07511125B2 Pyrimidine derivatives for labeled binding partners 失效
标题翻译：用于标记结合配偶体的嘧啶衍生物
公开(公告)号：US07511125B2
公开(公告)日：2009-03-31
申请号：US10921734
申请日：2004-08-19
申请人： Kuei-Ying Lin , Mark D. Matteucci
发明人： Kuei-Ying Lin , Mark D. Matteucci
IPC分类号： C07H19/00 , C07H21/00
CPC分类号： C07H19/06 , C07H21/00 , C12Q1/6813 , C12Q2525/101 , Y02P20/55 , C12Q2563/107
摘要： Compounds having structure (1) wherein R1 is —H a protecting group, a linker or a binding partner; and R2 and R34 are as defined in the specification. The invention also provides intermediates and methods make the structure (1) compounds, as well as methods to use the compounds as labels in diagnostic assays and to enhance binding to complementary bases.
摘要翻译：具有结构（1）的化合物，其中R 1为-H，保护基，连接基或结合配偶体; R2和R34如说明书中所定义。本发明还提供中间体和方法制备结构（1）化合物，以及在诊断测定中使用化合物作为标记并增强与互补碱基的结合的方法。

55. 发明申请

US20090075288A1 METHOD FOR DE NOVO DETECTION OF SEQUENCES IN NUCLEIC ACIDS: TARGET SEQUENCING BY FRAGMENTATION 有权
标题翻译：在核酸中检测序列的方法：通过分段进行的目标序列
公开(公告)号：US20090075288A1
公开(公告)日：2009-03-19
申请号：US12259376
申请日：2008-10-28
申请人： Charles R. Cantor , Fouad A. Siddiqi
发明人： Charles R. Cantor , Fouad A. Siddiqi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6872 , C12Q2525/101 , C12Q2523/107
摘要： The present invention provides a method for determining nucleic acid sequences of a template nucleic acid that requires no prior knowledge of the nucleic acid sequence present in the template nucleic acid. The method is based on combining information about the mass of a fragment, the mass of any one nucleotide and the combinations thereof, and the sequence specificity of a nucleotide cutter, either enzymatic or chemical cutter, to determine a sequence of a nucleic acid fragment. This method allows for de novo detection of sequences in a target nucleic acid without requiring any prior sequence information. This method is called Partial Sequencing by Fragmentation (PSBF) and it works by fragmenting a target into oligo- or polynucleotides whose masses or lengths are uniquely associated with known sequences. The identities of these sequences are determined solely by the specific fragmentation method used, and are always independent of the target. PSBF can be implemented using electrophoresis, mass spectrometry or any other method that can be used to distinguish the size of the cut nucleic acid sequence fragments.
摘要翻译：本发明提供了一种确定模板核酸的核酸序列的方法，其不需要模板核酸中存在的核酸序列的先验知识。该方法基于组合关于片段的质量，任何一个核苷酸的质量及其组合的信息以及核苷酸切割剂（酶或化学切割机）的序列特异性，以确定核酸片段的序列。该方法允许从头检测靶核酸中的序列，而不需要任何先前的序列信息。这种方法被称为通过片段化分段测序（PSBF），它的作用是通过将目标片段分成其质量或长度与已知序列唯一相关的寡核苷酸或多核苷酸。这些序列的身份完全由所使用的具体碎片方法确定，并且始终与目标无关。 PSBF可以使用电泳，质谱法或可用于区分切割的核酸序列片段的大小的任何其它方法来实现。

56. 发明申请

US20090018324A1 LABELED NUCLEOTIDE ANALOGS AND USES THEREFOR 有权
标题翻译：标记核苷类似物及其用途
公开(公告)号：US20090018324A1
公开(公告)日：2009-01-15
申请号：US12121641
申请日：2008-05-15
申请人： Yue Xu , Jeffrey Wegener , Arek Bibillo
发明人： Yue Xu , Jeffrey Wegener , Arek Bibillo
IPC分类号： C07H19/20
CPC分类号： C07H19/10 , C07H21/00 , C12Q1/6869 , C12Q2533/101 , C12Q2525/117 , C12Q2525/101
摘要： Labeled nucleotide analogs used in place of naturally occurring nucleoside triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions, and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays and others.
摘要翻译：在模板定向核酸合成反应和其他核酸反应中使用代替天然存在的核苷三磷酸或其他类似物的标记的核苷酸类似物，以及基于其的各种分析，包括DNA测序，单碱基鉴定，杂交测定等。

57. 发明授权

US07473767B2 Methods for detection and quantification of analytes in complex mixtures 有权
标题翻译：复杂混合物中分析物的检测和定量方法
公开(公告)号：US07473767B2
公开(公告)日：2009-01-06
申请号：US09898743
申请日：2001-07-03
申请人： Krassen Dimitrov
发明人： Krassen Dimitrov
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6888 , C12Q1/6816 , C12Q1/682 , C12Q1/6876 , C12Q2565/102 , C12Q2537/143 , C12Q2525/161 , C12Q2565/1025 , C12Q2563/179 , C12Q2525/151 , C12Q2525/101
摘要： The invention provides a diverse population of uniquely labeled probes, containing about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of producing a population of uniquely labeled nucleic acid probes. The method consists of (a) synthesizing a population of target specific nucleic acid probes each having a different specifier; (b) synthesizing a corresponding population of anti-genedigits each having a unique label, the population having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) hybridizing the populations of target nucleic acid probes to the anti-genedigits, to produce a population in which each of the target specific probes is uniquely labeled. Also provided is a method of detecting a nucleic acid analyte.
摘要翻译：本发明提供了多种独特标记的探针，其含有约30个或更多个目标特异性核酸探针，其各自附着于与核酸结合的唯一标记。还提供了生产独特标记的核酸探针群体的方法。该方法包括（a）合成具有不同说明符的目标特异性核酸探针群; （b）合成各自具有独特标记的相应抗原基因群体，所述群体具有足以独特地与指定物内的基因组杂交的多样性，和（c）将靶核酸探针的群体与抗Genieigits杂交，以产生其中每个靶特异性探针被唯一标记的群体。还提供了检测核酸分析物的方法。

58. 发明授权

US07459145B2 Multifunctional magnetic nanoparticle probes for intracellular molecular imaging and monitoring 失效
标题翻译：用于细胞内分子成像和监测的多功能磁性纳米颗粒探针
公开(公告)号：US07459145B2
公开(公告)日：2008-12-02
申请号：US10694243
申请日：2003-10-27
申请人： Gang Bao , Shuming Nie , Nitin Nitin , Leslie LaConte
发明人： Gang Bao , Shuming Nie , Nitin Nitin , Leslie LaConte
IPC分类号： A61B5/055
CPC分类号： C12Q1/6818 , A61K49/0002 , A61K49/0041 , A61K49/0093 , A61K49/1836 , A61K49/186 , B82Y5/00 , B82Y30/00 , C12Q1/6834 , C12Q1/6841 , G01N33/5005 , G01N33/587 , C12Q2563/155 , C12Q2563/143 , C12Q2525/301 , C12Q2525/101
摘要： The present invention provides multifunctional magnetic nanoparticle probe compositions for molecular imaging and monitoring, comprising a nucleic acid or polypeptide probe, a delivery ligand, and a magnetic nanoparticle having a biocompatible coating thereon. The probe compositions may further comprise a fluorescent or luminescent resonance energy transfer moiety. Also provided are compositions comprising two or more such multifunctional magnetic nanoparticle probes for molecular imaging or monitoring. In particular, the nucleic acid or polypeptide probes bind to a target and generate an interaction observable with magnetic resonance imaging (MRI) or optical imaging. The invention thereby provides detectable signals for rapid, specific, and sensitive detection of nucleic acids, polypeptides, and interactions thereof in vivo.
摘要翻译：本发明提供用于分子成像和监测的多功能磁性纳米颗粒探针组合物，其包含核酸或多肽探针，递送配体和其上具有生物相容性涂层的磁性纳米颗粒。探针组合物还可以包含荧光或发光共振能量转移部分。还提供了包含两种或更多种用于分子成像或监测的这种多功能磁性纳米颗粒探针的组合物。特别地，核酸或多肽探针结合靶并产生可用磁共振成像（MRI）或光学成像观察到的相互作用。因此，本发明提供可检测的信号，用于在体内快速，特异和灵敏地检测核酸，多肽及其相互作用。

59. 发明申请

US20080293580A1 Methods for Detecting and Measuring Specific Nucleic Acid Sequences 审中-公开
标题翻译：检测和测量特定核酸序列的方法
公开(公告)号：US20080293580A1
公开(公告)日：2008-11-27
申请号：US10578248
申请日：2004-11-08
申请人： Suk-Wah Tam-Chang , Kenneth W. Hunter , Nelson G. Publicover
发明人： Suk-Wah Tam-Chang , Kenneth W. Hunter , Nelson G. Publicover
IPC分类号： C40B30/04 , C40B40/08 , C07H21/00
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q1/6818 , C12Q1/6837 , C12Q2525/185 , C12Q2525/301 , C12Q2565/107 , C12Q2565/101 , C12Q2525/101
摘要： The invention provides novel oligonucleotides and methods of using the same for detection or measurement of specific nucleic acid molecules. The invention also features nucleic acid arrays comprising the oligonucleotides of the invention. An oligonucleotide of the invention comprises (1) a reporter-binding sequence capable of hybridizing to a fluorrophore-labeled reporter sequence and (2) a hairpin-forming sequence capable of forming a stem-loop. Formation of the stem-loop modifies (e.g., quenching) the fluorescence signals of the reporter sequence when the reporter sequence is hybridized to the oligonucleotide. This can be achieved, for example, by bringing one or more guanine based in the oligonucleotide into close proximity to the fluorophore(s) of the reporter sequence by virtue of the formation of the stem-loop. Disruption of the stem-loop, such as by hybridization of a target sequence to at least part of the hairpin-forming sequence, produces a detectable change in the fluorescence signals.
摘要翻译：本发明提供新的寡核苷酸及其用于检测或测量特异性核酸分子的方法。本发明还具有包含本发明寡核苷酸的核酸阵列。本发明的寡核苷酸包含（1）能够与荧光标记的报道基因序列杂交的报告基因结合序列和（2）能够形成茎环的发夹形成序列。当报告物序列与寡核苷酸杂交时，茎环的形成修饰（例如，淬灭）报告基因序列的荧光信号。这可以通过例如通过使一个或多个基于寡核苷酸的鸟嘌呤通过茎 - 环的形成使报告物序列的荧光团靠近来实现。茎环的破坏，例如通过目标序列与形成发夹的序列的至少部分的杂交产生荧光信号的可检测的变化。

60. 发明申请

US20080286837A1 Nucleotide analogs 有权
标题翻译：核苷酸类似物
公开(公告)号：US20080286837A1
公开(公告)日：2008-11-20
申请号：US11804591
申请日：2007-05-18
申请人： Suhaib Siddiqi
发明人： Suhaib Siddiqi
IPC分类号： C12P19/30 , C07H19/06 , C07H19/16
CPC分类号： C07H19/06 , A61K49/0052 , C07H19/16 , C12Q1/6874 , C12Q2525/101 , C12Q2525/197 , C12Q2563/107
摘要： The disclosure provides nucleotide analogs and methods of their use. Analogs of the invention comprise a reporter molecule (label) attached via the N4, N6, O4, or O6 position of the nitrogenous base portion of the analog. In a preferred embodiment, nucleotide analogs of the invention comprise a label attached to the nitrogenous base portion of the analog via a cleavable linker at the N4, O4, N6 or O6 position.
摘要翻译：本公开提供其使用的核苷酸类似物和方法。本发明的类似物包含通过类似物的含氮碱部分的N4，N6，O4或O6位置连接的报道分子（标记）。在优选的实施方案中，本发明的核苷酸类似物包含在N4，O4，N6或O6位置经由可切割接头连接到类似物的含氮碱部分的标记。

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