专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

51. 发明申请

US20100184074A1 UNIVERSAL NUCLEOTIDES FOR NUCLEIC ACID ANALYSIS 审中-公开
标题翻译：核酸分析通用核
公开(公告)号：US20100184074A1
公开(公告)日：2010-07-22
申请号：US12702239
申请日：2010-02-08
申请人： Barnett B. ROSENBLUM , Geun-Sook Jeon , Shaheer H. Khan
发明人： Barnett B. ROSENBLUM , Geun-Sook Jeon , Shaheer H. Khan
IPC分类号： C12Q1/68 , G01N27/26
CPC分类号： C12Q1/6869 , Y10S435/975 , C12Q2535/125 , C12Q2525/117 , C12Q2525/101 , C12Q2535/101
摘要： The invention includes methods and kits for making and analyzing primer extension products incorporating one or more universal bases, including methods and kits for nucleic acid sequencing and microsatellite analysis.
摘要翻译：本发明包括用于制备和分析引入一个或多个通用碱基的引物延伸产物的方法和试剂盒，包括用于核酸测序和微卫星分析的方法和试剂盒。

52. 发明申请

US20100133117A1 METHODS FOR ELECTROCHEMICAL DETECTION/QUANTIFICATION OF A NUCLEIC ACID 审中-公开
标题翻译：一种核酸的电化学检测/定量方法
公开(公告)号：US20100133117A1
公开(公告)日：2010-06-03
申请号：US12376782
申请日：2006-08-08
申请人： Zhiqiang Gao
发明人： Zhiqiang Gao
IPC分类号： C12Q1/28
CPC分类号： G01N27/447 , C12Q1/6825 , C12Q2525/107 , C12Q2533/101 , C12Q2525/101
摘要： The invention provides methods and kits for the electrochemical detection and/or quantification of a target nucleic acid molecule by means of a detection electrode. In one method there is immobilized on the detection electrode a peptide nucleic acid (PNA) capture molecule, which has a nucleotide sequence that is at least partially complementary to at least a portion of the target nucleic acid molecule. The electrode is contacted with a solution expected to include the target nucleic acid molecule and the target nucleic acid molecule allowed to hybridize to the PNA, thereby allowing the formation of a complex between the PNA capture molecule and the target nucleic acid molecule. A polymerisable positively chargeable precursor is added, which associates to the complex formed between the PNA capture molecule and the target nucleic acid molecule. A suitable reactant molecule is added, initiating the polymerisation of the polymerisable positively chargeable precursor and the formation of an electroconductive polymer. An electrochemical measurement at the detection electrode is performed. In another method a nucleic acid capture molecule is immobilized on the electrode. In this method a polymerisable positively chargeable precursor, a suitable substrate molecule, and an enzyme attached to a detection probe nucleic acid molecule are used. The detection probe nucleic acid molecule is at least partially complementary to at least a portion of the target nucleic acid molecule. The detection probe hybridizes to a portion of the target nucleic acid that is different from the portion to which the capture nucleic acid molecule hybridises.
摘要翻译：本发明提供了用于通过检测电极电化学检测和/或定量靶核酸分子的方法和试剂盒。在一种方法中，固定在检测电极上的肽核酸（PNA）捕获分子，其具有与目标核酸分子的至少一部分至少部分互补的核苷酸序列。使电极与预期包含靶核酸分子和允许与PNA杂交的靶核酸分子的溶液接触，从而允许在PNA捕获分子和靶核酸分子之间形成复合物。加入可聚合的带正电荷的前体，其与PNA捕获分子和靶核酸分子之间形成的复合物相关联。加入合适的反应物分子，引发可聚合的可带正电荷的前体和形成导电聚合物。进行检测电极的电化学测定。在另一种方法中，核酸捕获分子固定在电极上。在该方法中，使用可聚合的可带正电荷的前体，合适的底物分子和附着于检测探针核酸分子的酶。检测探针核酸分子与靶核酸分子的至少一部分至少部分互补。检测探针与靶核酸的不同于捕获核酸分子杂交的部分杂交。

53. 发明申请

US20100028890A1 COMPOSITIONS, METHODS, AND KITS FOR ANALYZING DNA METHYLATION 审中-公开
标题翻译：用于分析DNA甲基化的组合物，方法和试剂盒
公开(公告)号：US20100028890A1
公开(公告)日：2010-02-04
申请号：US12498314
申请日：2009-07-06
申请人： Victoria L. BOYD
发明人： Victoria L. BOYD
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6883 , C12Q1/6851 , C12Q1/6853 , C12Q2600/154 , C12Q2525/161 , C12Q2525/101 , C12Q2523/125
摘要： Compositions, methods, and kits for reducing strand amplification bias using bisulfite treated gDNA are provided. Methods for detecting and for quantitating the amplified bisulfite treated gDNA and inferring the presence, absence, and/or degree of methylation of target cytosine(s) in the gDNA are also provided. Such methods typically employ tailed first primer pairs, which can, but need not comprise nucleotide analogs, and optionally second primer pairs.
摘要翻译：提供了使用亚硫酸氢盐处理的gDNA减少链扩增偏差的组合物，方法和试剂盒。还提供了用于检测和定量扩增的亚硫酸氢盐处理的gDNA并推断出gDNA中靶胞嘧啶甲基化的存在，不存在和/或程度的方法。这样的方法通常采用尾部的第一引物对，其可以但不必包含核苷酸类似物和任选的第二引物对。

54. 发明申请

US20100003724A1 CHEMICALLY MODIFIED NUCLEOSIDE 5'-TRIPHOSPHATES FOR THERMALLY INITIATED AMPLIFICATION OF NUCLEIC ACID 有权
标题翻译：化学改性核壳素5'-磷酸酯，用于热启动核酸扩增
公开(公告)号：US20100003724A1
公开(公告)日：2010-01-07
申请号：US12470449
申请日：2009-05-21
申请人： Alexandre Lebedev , Inna Koukhareva
发明人： Alexandre Lebedev , Inna Koukhareva
IPC分类号： C12P19/34
CPC分类号： C07H19/00 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q2525/101 , C12Q2525/186 , C12Q2545/101
摘要： Provided herein are methods and compositions for nucleic acid replication. These methods involve the use of 3′-substituted nucleoside 5′-triphosphates or 3′-substituted terminated primers in nucleic acid replication reactions. In certain aspects, the methods are accomplished by use of 3′-substituted NTPs and/or 3′-substituted terminated primers which provide utility in nucleic acid replication. In preferred embodiments, the NTPs and/or primers are substituted at the 3′-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.
摘要翻译：本文提供了用于核酸复制的方法和组合物。这些方法涉及在核酸复制反应中使用3'-取代核苷5'-三磷酸或3'-取代的终止引物。在某些方面，所述方法通过使用在核酸复制中提供效用的3'-取代的NTP和/或3'-取代的封端引物来实现。在优选的实施方案中，NTP和/或引物在3'-位置被特定的热不稳定化学基团例如醚，酯或碳酸酯代替。

55. 发明授权

US07622279B2 Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry 有权
标题翻译：用于通过位点特异性偶联化学构建的芯片上的DNA测序的可透光荧光核苷酸
公开(公告)号：US07622279B2
公开(公告)日：2009-11-24
申请号：US10591520
申请日：2005-03-03
申请人： Jingyue Ju
发明人： Jingyue Ju
IPC分类号： C12P19/34 , C12Q1/68 , C07G3/00 , C07H19/04 , C07H21/00 , C07H21/02
CPC分类号： C12Q1/6874 , C12Q2525/101 , C12Q2523/319
摘要： This invention provides a method for determining the sequence of a DNA or an RNA, wherein (i) about 1000 or fewer copies of the DNA or RNA are bound to a solid substrate via 1,3-dipolar azide-alkyne cycloaddition chemistry and (ii) each copy of the DNA or RNA comprises a self-priming moiety.
摘要翻译：本发明提供了一种用于确定DNA或RNA序列的方法，其中（i）约1000个或更少拷贝的DNA或RNA通过1,3-偶极叠氮化物 - 炔环化学化学结合到固体底物上，和（ii））DNA或RNA的每个拷贝包含自引发部分。

56. 发明授权

US07541147B2 Materials and methods for detection of nucleic acids 有权
公开(公告)号：US07541147B2
公开(公告)日：2009-06-02
申请号：US11546631
申请日：2006-10-12
申请人： David J. Marshall , James R. Prudent , Christopher B. Sherrill , Gideon Shapiro , Simona Jurczyk , Jerod L. Ptacin
发明人： David J. Marshall , James R. Prudent , Christopher B. Sherrill , Gideon Shapiro , Simona Jurczyk , Jerod L. Ptacin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6823 , C12Q1/6827 , C12Q1/6832 , C12Q1/6834 , C12Q1/6858 , C12Q2600/156 , C12Q2565/1015 , C12Q2525/101 , C12Q2525/161
摘要： Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.

57. 发明授权

US07511125B2 Pyrimidine derivatives for labeled binding partners 失效
标题翻译：用于标记结合配偶体的嘧啶衍生物
公开(公告)号：US07511125B2
公开(公告)日：2009-03-31
申请号：US10921734
申请日：2004-08-19
申请人： Kuei-Ying Lin , Mark D. Matteucci
发明人： Kuei-Ying Lin , Mark D. Matteucci
IPC分类号： C07H19/00 , C07H21/00
CPC分类号： C07H19/06 , C07H21/00 , C12Q1/6813 , C12Q2525/101 , Y02P20/55 , C12Q2563/107
摘要： Compounds having structure (1) wherein R1 is —H a protecting group, a linker or a binding partner; and R2 and R34 are as defined in the specification. The invention also provides intermediates and methods make the structure (1) compounds, as well as methods to use the compounds as labels in diagnostic assays and to enhance binding to complementary bases.
摘要翻译：具有结构（1）的化合物，其中R 1为-H，保护基，连接基或结合配偶体; R2和R34如说明书中所定义。本发明还提供中间体和方法制备结构（1）化合物，以及在诊断测定中使用化合物作为标记并增强与互补碱基的结合的方法。

58. 发明申请

US20090075288A1 METHOD FOR DE NOVO DETECTION OF SEQUENCES IN NUCLEIC ACIDS: TARGET SEQUENCING BY FRAGMENTATION 有权
标题翻译：在核酸中检测序列的方法：通过分段进行的目标序列
公开(公告)号：US20090075288A1
公开(公告)日：2009-03-19
申请号：US12259376
申请日：2008-10-28
申请人： Charles R. Cantor , Fouad A. Siddiqi
发明人： Charles R. Cantor , Fouad A. Siddiqi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6872 , C12Q2525/101 , C12Q2523/107
摘要： The present invention provides a method for determining nucleic acid sequences of a template nucleic acid that requires no prior knowledge of the nucleic acid sequence present in the template nucleic acid. The method is based on combining information about the mass of a fragment, the mass of any one nucleotide and the combinations thereof, and the sequence specificity of a nucleotide cutter, either enzymatic or chemical cutter, to determine a sequence of a nucleic acid fragment. This method allows for de novo detection of sequences in a target nucleic acid without requiring any prior sequence information. This method is called Partial Sequencing by Fragmentation (PSBF) and it works by fragmenting a target into oligo- or polynucleotides whose masses or lengths are uniquely associated with known sequences. The identities of these sequences are determined solely by the specific fragmentation method used, and are always independent of the target. PSBF can be implemented using electrophoresis, mass spectrometry or any other method that can be used to distinguish the size of the cut nucleic acid sequence fragments.
摘要翻译：本发明提供了一种确定模板核酸的核酸序列的方法，其不需要模板核酸中存在的核酸序列的先验知识。该方法基于组合关于片段的质量，任何一个核苷酸的质量及其组合的信息以及核苷酸切割剂（酶或化学切割机）的序列特异性，以确定核酸片段的序列。该方法允许从头检测靶核酸中的序列，而不需要任何先前的序列信息。这种方法被称为通过片段化分段测序（PSBF），它的作用是通过将目标片段分成其质量或长度与已知序列唯一相关的寡核苷酸或多核苷酸。这些序列的身份完全由所使用的具体碎片方法确定，并且始终与目标无关。 PSBF可以使用电泳，质谱法或可用于区分切割的核酸序列片段的大小的任何其它方法来实现。

59. 发明申请

US20090018324A1 LABELED NUCLEOTIDE ANALOGS AND USES THEREFOR 有权
标题翻译：标记核苷类似物及其用途
公开(公告)号：US20090018324A1
公开(公告)日：2009-01-15
申请号：US12121641
申请日：2008-05-15
申请人： Yue Xu , Jeffrey Wegener , Arek Bibillo
发明人： Yue Xu , Jeffrey Wegener , Arek Bibillo
IPC分类号： C07H19/20
CPC分类号： C07H19/10 , C07H21/00 , C12Q1/6869 , C12Q2533/101 , C12Q2525/117 , C12Q2525/101
摘要： Labeled nucleotide analogs used in place of naturally occurring nucleoside triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions, and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays and others.
摘要翻译：在模板定向核酸合成反应和其他核酸反应中使用代替天然存在的核苷三磷酸或其他类似物的标记的核苷酸类似物，以及基于其的各种分析，包括DNA测序，单碱基鉴定，杂交测定等。

60. 发明授权

US07473767B2 Methods for detection and quantification of analytes in complex mixtures 有权
标题翻译：复杂混合物中分析物的检测和定量方法
公开(公告)号：US07473767B2
公开(公告)日：2009-01-06
申请号：US09898743
申请日：2001-07-03
申请人： Krassen Dimitrov
发明人： Krassen Dimitrov
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6888 , C12Q1/6816 , C12Q1/682 , C12Q1/6876 , C12Q2565/102 , C12Q2537/143 , C12Q2525/161 , C12Q2565/1025 , C12Q2563/179 , C12Q2525/151 , C12Q2525/101
摘要： The invention provides a diverse population of uniquely labeled probes, containing about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of producing a population of uniquely labeled nucleic acid probes. The method consists of (a) synthesizing a population of target specific nucleic acid probes each having a different specifier; (b) synthesizing a corresponding population of anti-genedigits each having a unique label, the population having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) hybridizing the populations of target nucleic acid probes to the anti-genedigits, to produce a population in which each of the target specific probes is uniquely labeled. Also provided is a method of detecting a nucleic acid analyte.
摘要翻译：本发明提供了多种独特标记的探针，其含有约30个或更多个目标特异性核酸探针，其各自附着于与核酸结合的唯一标记。还提供了生产独特标记的核酸探针群体的方法。该方法包括（a）合成具有不同说明符的目标特异性核酸探针群; （b）合成各自具有独特标记的相应抗原基因群体，所述群体具有足以独特地与指定物内的基因组杂交的多样性，和（c）将靶核酸探针的群体与抗Genieigits杂交，以产生其中每个靶特异性探针被唯一标记的群体。还提供了检测核酸分析物的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式