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51. 发明授权

US12060570B2 Vector-free intracellular delivery by reversible permeabilisation 有权
公开(公告)号：US12060570B2
公开(公告)日：2024-08-13
申请号：US17337165
申请日：2021-06-02
申请人： Avectas Limited
发明人： Michael Maguire , Shirley O'Dea
IPC分类号： C12N15/87 , B01J19/00 , B01L3/00 , C07K14/725 , C12M1/00 , C12M1/32 , C12M1/42 , C12M3/00 , C12M3/02 , C12N5/0783 , C12N13/00 , C12N15/113
CPC分类号： C12N15/87 , B01L3/5027 , C07K14/7051 , C12M3/006 , C12M3/02 , C12M35/00 , C12M35/04 , C12N5/0636 , C12N13/00 , C12N15/113 , B01J19/0026 , C07K2319/03 , C12M23/12 , C12M29/06 , C12M41/14
摘要： The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

52. 发明授权

US11981915B2 Vector-free intracellular delivery by reversible permeabilization 有权
公开(公告)号：US11981915B2
公开(公告)日：2024-05-14
申请号：US16471466
申请日：2017-12-21
申请人： Avectas Limited
发明人： Michael Maguire , Shirley O'Dea
IPC分类号： C12N15/87 , B01J19/00 , B01L3/00 , C07K14/725 , C12M1/00 , C12M1/32 , C12M1/42 , C12M3/00 , C12M3/02 , C12N5/0783 , C12N13/00 , C12N15/113
CPC分类号： C12N15/87 , B01L3/5027 , C07K14/7051 , C12M3/006 , C12M3/02 , C12M35/00 , C12M35/04 , C12N5/0636 , C12N13/00 , C12N15/113 , B01J19/0026 , C07K2319/03 , C12M23/12 , C12M29/06 , C12M41/14
摘要： The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

53. 发明公开

US20230303961A1 SAMPLE MEASUREMENT APPARATUS, SAMPLE MEASUREMENT SYSTEM, AND ARTIFICIAL INSEMINATION APPARATUS 审中-公开
公开(公告)号：US20230303961A1
公开(公告)日：2023-09-28
申请号：US18179557
申请日：2023-03-07
申请人： Hitachi, Ltd. , National Institutes for Quantum Science and Technology
发明人： Masanari KOGUCHI , Takeshi NAKAYAMA , Ryuji IGARASHI , Kiichi KAMINAGA , Satoshi TSUKAMOTO
IPC分类号： C12M1/34 , C12M1/12 , C12M1/42 , G01N21/64
CPC分类号： C12M41/46 , C12M25/10 , C12M35/00 , G01N21/6428
摘要： An object of the invention is to make it possible to provide a mechanism that applies an external stimulus and to measure structural and electromagnetic changes of a cell due to the external stimulus with high sensitivity by an NV center. There is provided an environment control mechanism configured to change a state of a sample by applying an external stimulus to the sample.

54. 发明申请

US20190169605A1 AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS 审中-公开
公开(公告)号：US20190169605A1
公开(公告)日：2019-06-06
申请号：US16269655
申请日：2019-02-07
申请人： Inscripta, Inc.
发明人： Don Masquelier , Phillip Belgrader , Jorge Bernate , Ryan Gill , Kevin Ness
IPC分类号： C12N15/10 , C40B40/02 , C12M1/36 , C12N15/11 , C12M3/00 , C12M1/12 , C12M1/00 , C12N9/22
CPC分类号： C12N15/1082 , C12M23/44 , C12M33/14 , C12M35/00 , C12M37/04 , C12M41/36 , C12M41/48 , C12M43/00 , C12N9/22 , C12N15/11 , C12N2310/20 , C12N2800/80 , C40B40/02
摘要： In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

55. 发明申请

US20180003696A1 GENE EDITING THROUGH MICROFLUIDIC DELIVERY 审中-公开
公开(公告)号：US20180003696A1
公开(公告)日：2018-01-04
申请号：US15542892
申请日：2016-01-12
申请人： Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： Armon R. SHAREI , Marc LAJOIE , Klavs F. JENSEN , Robert S. LANGER
IPC分类号： G01N33/50 , C12N15/87 , G01N33/487 , C12Q1/00
CPC分类号： G01N33/5002 , C12M35/00 , C12N15/87 , C12Q1/00 , G01N33/48721
摘要： Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described. The methods and systems of the invention solve the problem of intracellular delivery of gene editing components and gene editing complexes to target cells. The results described herein indicate that delivery of gene editing components, e.g., protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), by mechanical disruption of cell membranes leads to successful gene editing. Because intracellular delivery of gene editing materials is a current challenge, the methods provide a robust mechanism to engineer target cells without the use of potentially harmful viral vectors or electric fields.

56. 发明申请

US20170130238A1 DEVICE AND METHODS FOR BIOLISTIC TRANSFORMATION 审中-公开
公开(公告)号：US20170130238A1
公开(公告)日：2017-05-11
申请号：US15343064
申请日：2016-11-03
申请人： SYNTHETIC GENOMICS, INC.
发明人： Amanda R. Edwards , John H. Verruto
IPC分类号： C12N15/82
CPC分类号： C12N15/8207 , C12M35/00 , C12M35/04 , C12N15/895
摘要： The present invention provides an innovate method and device for depositing coated particles to prepare sample cartridges for use in a gene gun. It allows for scalability of sample cartridge preparation, as well as the ability to prepare multiple different samples simultaneously thereby reducing the time required to prepare different samples and consequently the time required to perform a biological assay, such as a transformation as

57. 发明申请

US20150191686A1 Purification Methods and Systems Related to Renewable Materials and Biofuels Production 审中-公开
标题翻译：与可再生材料和生物燃料生产相关的净化方法和系统
公开(公告)号：US20150191686A1
公开(公告)日：2015-07-09
申请号：US14597511
申请日：2015-01-15
申请人： BP Corporation North America Inc.
发明人： Glen Austin , Binita Bhattacharjee , Leslie Bolton , Jacob Borden , Martin Carrera , Amit Gokhale , Chris Horler , Aidan Hurley , Eric Mack
IPC分类号： C12M1/00 , C12M1/42 , C12P7/16
CPC分类号： C12M21/12 , C12M35/00 , C12M43/02 , C12M47/10 , C12P5/00 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/649 , Y02E50/10 , Y02E50/13 , Y02E50/17 , Y02T50/678
摘要： Methods of producing renewable materials may include consuming a fermentation feedstock with a fermentation organism to produce a renewable material in fermentation broth; water may then be separated from the feedstock or broth using one or more phase separations, or the renewable material may be concentrated from the feedstock or broth using one or more phase separations. Methods of producing biofuel components may include consuming a lignocellulosic or sugar fermentation feedstock with a fermentation organism to produce either ethanol or butanol in fermentation broth; cooling the feedstock or broth to solidify at least some water therein; and separating the solidified water from the feedstock or broth using a solid-liquid phase separation.
摘要翻译：生产可再生材料的方法可以包括用发酵生物体消耗发酵原料以在发酵液中产生可再生材料; 然后可以使用一个或多个相分离将水与原料或肉汤分离，或者使用一个或多个相分离可以从原料或肉汤浓缩可再生材料。生产生物燃料组分的方法可以包括用发酵生物体消耗木质纤维素或糖发酵原料，以在发酵液中产生乙醇或丁醇; 冷却原料或肉汤以固化至少一些水; 并使用固 - 液相分离从原料或肉汤中分离凝固的水。

58. 发明申请

US20150150203A1 BIOREACTOR VESSEL FOR LARGE SCALE GROWING OF PLANTS UNDER ASEPTIC CONDITION 有权
标题翻译：生物反应器船只在大气压条件下大规模生长植物
公开(公告)号：US20150150203A1
公开(公告)日：2015-06-04
申请号：US14415711
申请日：2013-08-01
申请人： Council of Scientific & Industrial Research
发明人： Rajesh Thakur , Anil Sood , Paramvir Singh Ahuja
IPC分类号： A01G31/02
CPC分类号： A01G31/02 , A01G9/16 , A01H4/001 , C12M21/08 , C12M35/00 , C12M35/04 , Y02A40/254
摘要： An apparatus is used for growing plants or tissues under aseptic conditions. The apparatus includes a lid and a base compartment. A linking mechanism is provided for connecting the lid and the base compartment. The linking mechanism is provided with a locking mechanism, which is movable from a locked position to an un-locked position and vice-versa. In the locked position, the locking mechanism holds the lid in an abutting closed relation with respect to the base compartment. In the un-locked position, the linking mechanism is provided with means for automatically lifting the lid to an elevated-suspended position with respect to the base compartment thereby providing an access route to reach the base compartment and perform desired operations.
摘要翻译：一种装置用于在无菌条件下种植植物或组织。该装置包括一个盖子和一个基座隔间。提供连接机构用于连接盖子和基座隔间。连接机构设置有锁定机构，其可以从锁定位置移动到非锁定位置，反之亦然。在锁定位置，锁定机构将盖子相对于基部隔间保持在邻接的关系。在未锁定位置，连接机构设置有用于将盖子相对于基部隔间自动提升到升高的悬挂位置的装置，从而提供进入基座隔间并进行所需操作的通道。

59. 发明授权

US08990023B2 High-throughput automated cellular injection system and method 有权
标题翻译：高通量自动细胞注射系统和方法
公开(公告)号：US08990023B2
公开(公告)日：2015-03-24
申请号：US11678608
申请日：2007-02-25
申请人： Yu Sun , Wenhui Wang , Xinyu Liu
发明人： Yu Sun , Wenhui Wang , Xinyu Liu
IPC分类号： C12M1/00 , G01N35/10 , C12M1/42 , C12M1/36
CPC分类号： C12M23/50 , C12M35/00 , C12M41/48
摘要： An automated cell injection system and method are described, which can perform automatic, reliable, and high-throughput cell injection of foreign genetic materials, proteins, and other compounds. The system and method overcome the problems inherent in traditional manual injection that is characterized by poor reproducibility, human fatigue, and low throughput. The present invention is particularly suited for zebrafish embryo injection but can be readily extended to other biological injection applications such as mouse embryo, drosophila embryo, and C. elegans injections, capable of facilitating high-throughput genetic research at both academic and industry levels. A novel vacuum based cell-holding device is also provided.
摘要翻译：描述了自动细胞注射系统和方法，其可以执行外来遗传物质，蛋白质和其他化合物的自动，可靠和高通量的细胞注射。该系统和方法克服了传统手动注射固有的问题，其特点是重现性差，人体疲劳和生产能力低。本发明特别适用于斑马鱼胚胎注射，但可以容易地扩展到其他生物注射应用例如小鼠胚胎，果蝇胚和秀丽隐杆线虫注射液，其能够促进学术和工业水平的高通量遗传研究。还提供了一种新颖的基于真空的电池保持装置。

60. 发明申请

US20140353172A1 DEVICES AND METHODS FOR LONG-TERM INTRACELLULAR ACCESS 有权
标题翻译：用于长期细胞内接入的装置和方法
公开(公告)号：US20140353172A1
公开(公告)日：2014-12-04
申请号：US14461303
申请日：2014-08-15
申请人： Nicholas Alexander MELOSH , Piyush VERMA , Benjamin David ALMQUIST
发明人： Nicholas Alexander MELOSH , Piyush VERMA , Benjamin David ALMQUIST
IPC分类号： G01N27/327
CPC分类号： C12M35/00 , G01N33/48728
摘要： Nanoscale probes for forming stable, non-destructive seals with cell membranes. The probes, systems including these probes, and methods of fabricating and using the probes described herein may be used to sense from, stimulate, modify, or otherwise effect individual cells or groups of cells. In particular, described herein are nanoscale cellular probes that may be used to fuse with, and in some variations span, the lipid membrane of a cell to provide stable and long lasting contact to the cell. Thus, the probes described herein may be used as part of a system, method or device that would benefit from stable, non-destructive access to and across a cell membrane. In some variations the nanoscale probe devices or systems described herein may be used as part of a drug screening procedure.
摘要翻译：纳米级探针，用于与细胞膜形成稳定的无损密封。包括这些探针的探针，系统以及制造和使用本文所述的探针的方法可以用于感测，刺激，修饰或以其他方式影响单个细胞或细胞群。特别地，本文所述是纳米级细胞探针，其可以用于与细胞的脂质膜融合，并且在一些变化中与细胞的脂质膜融合，以向细胞提供稳定和持久的接触。因此，本文描述的探针可以用作将受益于稳定的，非破坏性地进入和穿过细胞膜的系统，方法或装置的一部分。在一些变型中，本文所述的纳米级探针装置或系统可以用作药物筛选程序的一部分。

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