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    • 52. 发明申请
    • Modified Cry3A toxins
    • 改良的Cry3A毒素
    • US20060085870A1
    • 2006-04-20
    • US11294220
    • 2005-12-05
    • Eric ChenCheryl Stacy
    • Eric ChenCheryl Stacy
    • A01H1/00C12N15/82C07K14/325
    • C12N15/8286C07K14/325Y02A40/162
    • Compositions and methods for controlling plant pests are disclosed. In particular, novel nucleic acid sequences encoding modified Cry3A toxins having increased toxicity to corn rootworm are provided. By inserting a protease recognition site, such as cathepsin G, that is recognized by a gut protease of a target insect in at least one position of a Cry3A toxin a modified Cry3A toxin having significantly greater toxicity, particularly to western and northern corn rootworm is designed. Further, a method of making the modified Cry3A toxins and methods of using the modified cry3A nucleic acid sequences, for example in microorganisms to control insects or in transgenic plants to confer protection from insect damage, and a method of using the modified Cry3A toxins, and compositions and formulations comprising the modified Cry3A toxins, for example applying the modified Cry3A toxins or compositions or formulations to insect-infested areas, or to prophylactically treat insect-susceptible areas or plants to confer protection against the insect pests are disclosed.
    • 公开了用于控制植物害虫的组合物和方法。 特别地,提供了对玉米根虫具有增加的毒性的编码修饰的Cry3A毒素的新型核酸序列。 通过在蛋白酶识别位点(例如组织蛋白酶G)中插入由至少一种Cry3A毒素的至少一种位点的目标昆虫的肠蛋白酶识别出具有显着更大毒性的改良Cry3A毒素(特别是西部和北部玉米根虫)的蛋白酶识别位点 。 此外,制备修饰的Cry3A毒素的方法和使用经修饰的cry3A核酸序列的方法,例如在微生物中控制昆虫或在转基因植物中赋予防御昆虫损伤的方法,以及使用经修饰的Cry3A毒素的方法,以及 公开了包含修饰的Cry3A毒素的组合物和制剂,例如将经修饰的Cry3A毒素或组合物或制剂施用于昆虫侵袭区域,或用于预防性治疗昆虫敏感区域或植物以赋予对昆虫害虫的保护。