专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

51. 发明授权

US07198937B2 Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权
标题翻译：高山被孢霉二酰基甘油酰基转移酶用于改变多不饱和脂肪酸和含油生物中的油含量
公开(公告)号：US07198937B2
公开(公告)日：2007-04-03
申请号：US11024545
申请日：2004-12-29
申请人： Zhixiong Xue , Narendra S. Yadav , Daniel Joseph Macool
发明人： Zhixiong Xue , Narendra S. Yadav , Daniel Joseph Macool
IPC分类号： C12N1/16 , C12N1/13 , C12N1/19 , C12N1/18 , C12N9/10 , C12P21/06 , C07H21/04
CPC分类号： C12P7/6472 , C12N9/1029 , C12P7/6463
摘要： An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi and yeast and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. Most desirably, the substrate specificity of the instant DGAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.
摘要翻译：提供了一种酰基转移酶，适用于制造含油生物体中富含ω-脂肪酸的微生物油。具体地，从高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT2）的基因。该基因编码参与真菌和酵母中油生物合成的最终步骤的酶，并且预期在改变油性生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。最理想的是，即时DGAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母中积累长链等于或大于C 20的长链PUFA。

52. 发明授权

US06632980B1 Binary viral expression system in plants 失效
标题翻译：植物二元病毒表达系统
公开(公告)号：US06632980B1
公开(公告)日：2003-10-14
申请号：US09442021
申请日：1999-11-17
申请人： Narendra S. Yadav , S. Carl Falco
发明人： Narendra S. Yadav , S. Carl Falco
IPC分类号： C12N1582
CPC分类号： C07K14/005 , A61K47/60 , A61K47/62 , C07K17/08 , C12N15/8203 , C12N15/8209 , C12N15/8213 , C12N15/8216 , C12N15/8217 , C12N15/8222 , C12N15/8237 , C12N15/8238 , C12N15/8289 , C12N2750/12022 , C12N2770/00022 , C12N2770/40022
摘要： The invention relates to two plant transgene expression systems. The first is comprised of two chromosomally-integrated components that are individually heritable. One component is an inactive replicon, which contains cis-acting viral sequences required for replication and is unable to replicate episomally. The other component is a chimeric transactivating gene comprising a regulated promoter operably-linked to the coding region for a protein that can transactivate replicon replication. Regulated expression of the transactivation protein in plant cells containing the inactive replicon triggers release of free replicon from the integrated inactive replicon and allows episomal replication.
摘要翻译：本发明涉及两种植物转基因表达系统。第一个由两个独立于遗传的染色体整合成分组成。一个组分是一个非活性的复制子，其中包含复制所需的顺式作用病毒序列，并且无法以阴离子型复制。另一组分是嵌合反式激活基因，其包含可操作地连接到可以反式复制子复制的蛋白质的编码区的调节启动子。含有非活性复制子的植物细胞中的反式激活蛋白的调节表达引发了来自整合的无活性复制子的游离复制子的释放并允许附加型复制。

53. 发明申请

US20090274816A1 DELTA-15 DESATURASE GENES SUITABLE FOR INCREASING LEVELS OF OMEGA-3 FATTY ACIDS 有权
标题翻译：适用于增加OMEGA-3脂肪酸水平的DELTA-15去饱和基因
公开(公告)号：US20090274816A1
公开(公告)日：2009-11-05
申请号：US12420345
申请日：2009-04-08
申请人： Howard Glenn Damude , Narendra S. Yadav
发明人： Howard Glenn Damude , Narendra S. Yadav
IPC分类号： A23K1/16 , C12N15/74 , A01H5/00 , C11B1/00 , A01H1/00
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.
摘要翻译：本发明涉及能够催化亚油酸（18:2，LA）转化为α-亚麻酸（18:3，ALA）的真菌Δ-15脂肪酸去饱和酶。描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主植物和微生物。本文还描述了通过过表达Δ-15脂肪酸去饱和酶增加特定ω-3和ω-6脂肪酸的产生的方法。

54. 发明授权

US6077992A Binary viral expression system in plants 有权
标题翻译：植物二元病毒表达系统
公开(公告)号：US6077992A
公开(公告)日：2000-06-20
申请号：US178089
申请日：1998-10-23
申请人： Narendra S. Yadav
发明人： Narendra S. Yadav
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10 , C12N15/90
CPC分类号： C12N15/8217 , C12N15/8203
摘要： This invention relates to a regulated binary plant viral expression system. It is comprised of two chromosomally-integrated components. One component is a proreplicon, which contains cis-acting viral sequences required for replication and a contains a target gene. The other component is a chimeric trans-acting replication gene comprising a regulated promoter operably-linked to the coding region for a viral replication protein. The proreplicon lacks the replication gene essential for replicon replication, and thus cannot undergo autonomous episomal replication. However, regulated expression of the trans-acting replication protein in plant cells also containing the proreplicon will trigger the release of free replicon from the integrated proreplicon, result in its episomal replication in trans, and result in the expression of the target gene, if present, through gene amplification. The expression system is useful for both production of foreign proteins as well as silencing endogenous genes and transgenes in plant tissue. Tissue-specific expression is controlled by the choice of promoter controlling the transcription of the trans-acting replication gene.
摘要翻译：本发明涉及调节二元植物病毒表达系统。它由两个染色体整合的组件组成。一个组分是一个proreplicon，其包含复制所需的顺式作用病毒序列和含有靶基因。另一组分是嵌合反式作用复制基因，其包含可操作地连接于病毒复制蛋白的编码区的调节启动子。复制子缺乏复制子复制必需的复制基因，因此不能进行自发性增殖复制。然而，也含有副反应蛋白的植物细胞中的反式作用复制蛋白的调控表达将触发从整合的复制子释放游离的复制子，导致其反式的附加型复制，并导致靶基因的表达（如果存在），通过基因扩增。表达系统对于外源蛋白的生产以及沉默植物组织中的内源基因和转基因是有用的。组织特异性表达是通过控制反式作用复制基因转录的启动子的选择来控制的。

55. 发明授权

US5760206A Nucleotide sequence of soybean stearoyl-ACP desaturase gene 失效
公开(公告)号：US5760206A
公开(公告)日：1998-06-02
申请号：US474587
申请日：1995-06-07
申请人： William D. Hitz , Narendra S. Yadav , Luis Perez-Grau
发明人： William D. Hitz , Narendra S. Yadav , Luis Perez-Grau
IPC分类号： C12N9/02 , C12N15/82 , C12N15/11 , C12N5/14 , C12N15/00
CPC分类号： C12N9/0083 , C12N15/8247
摘要： The preparation and use of nucleic acid fragments encoding soybean seed stearoyl-ACP desaturase enzyme or its precursor to modify plant oil composition are described. Chimeric genes incorporating such nucleic acid fragments and suitable regulatory sequences may be utilized to transform plants to control the levels of saturated and unsaturated fatty acids.

56. 发明授权

US5378824A Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase 失效
标题翻译：编码抗除草剂植物乙酰乳酸合成酶的核酸片段
公开(公告)号：US5378824A
公开(公告)日：1995-01-03
申请号：US892305
申请日：1992-06-02
申请人： John R. Bedbrook , Roy S. Chaleff , Saverio C. Falco , Barbara J. Mazur , Christopher R. Somerville , Narendra S. Yadav
发明人： John R. Bedbrook , Roy S. Chaleff , Saverio C. Falco , Barbara J. Mazur , Christopher R. Somerville , Narendra S. Yadav
IPC分类号： C12N9/88 , C12N15/82 , C07H21/04 , C12N15/00 , C12P21/00 , C12P21/04
CPC分类号： C12N15/8274 , C12N15/8278 , C12N9/88
摘要： A nucleic acid fragment encoding a herbicide-resistant plant acetolactate synthase protein is disclosed. This nucleic acid fragment contains at least one nucleotide mutation resulting in one amino acid change in one of seven substantially conserved regions of acetolactate synthase amino acid homology. This mutation results in the production of an acetolactate synthase protein which is resistant to sulfonylurea herbicide compounds compared to the wild-type protein. Transformation of herbicide sensitive plants or plant cells with the fragment results in resistance to the herbicide.
摘要翻译：公开了编码除草剂抗性植物乙酰乳酸合酶蛋白的核酸片段。该核酸片段含有至少一个核苷酸突变，导致乙酰乳酸合酶氨基酸同源性的七个基本上保守的区域之一发生一个氨基酸变化。与野生型蛋白质相比，该突变导致对磺酰脲类除草剂化合物具有抗性的乙酰乳酸合酶蛋白质的产生。用片段转化除草剂敏感植物或植物细胞导致对除草剂的抗性。

57. 发明授权

US08273957B2 Δ-15 desaturase genes suitable for increasing levels of omega-3 fatty acids 有权
标题翻译：适用于提高ω-3脂肪酸水平的去饱和酶基因
公开(公告)号：US08273957B2
公开(公告)日：2012-09-25
申请号：US12420345
申请日：2009-04-08
申请人： Howard Glenn Damude , Narendra S. Yadav
发明人： Howard Glenn Damude , Narendra S. Yadav
IPC分类号： A01H5/00
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.
摘要翻译：本发明涉及能够催化亚油酸（18:2，LA）转化为α-亚麻酸（18:3，ALA）的真菌和Dgr-15脂肪酸去饱和酶。描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主植物和微生物。本文还描述了通过过量表达＆Dgr-15脂肪酸去饱和酶来增加特定ω-3和ω-6脂肪酸的产生的方法。

58. 发明申请

US20120151621A1 GENES FOR MICROSOMAL DELTA-12 FATTY ACID DESATURASES AND HYDROXYLASES FROM PLANTS 审中-公开
标题翻译：微生物DELTA-12脂肪酸脱氢酶和植物羟基的基因
公开(公告)号：US20120151621A1
公开(公告)日：2012-06-14
申请号：US12894935
申请日：2010-09-30
申请人： Jonathan Edward Lightner , John Joseph Okuley , William Dean Hitz , Anthony John Kinney , Luis Perez-Grau , Narendra S. Yadav
发明人： Jonathan Edward Lightner , John Joseph Okuley , William Dean Hitz , Anthony John Kinney , Luis Perez-Grau , Narendra S. Yadav
IPC分类号： A01H1/02 , A01H5/00 , A01H1/00 , C12Q1/68 , C07H21/04
CPC分类号： C12N9/0083 , A01H1/04 , A01H5/10 , A23D7/00 , A23D9/00 , C12N15/1137 , C12N15/8247 , C12N2310/11 , C12Y114/19006 , G01N31/22
摘要： The preparation and use of nucleic acid fragments encoding fatty acid desaturase enzymes are described. The invention permits alteration of plant lipid composition. Chimeric genes incorporating such nucleic acid fragments with suitable regulatory sequences may be used to create transgenic plants with altered levels of unsaturated fatty acids.
摘要翻译：描述了编码脂肪酸去饱和酶的核酸片段的制备和使用。本发明允许改变植物脂质组成。使用合适的调控序列掺入此类核酸片段的嵌合基因可用于产生具有改变水平的不饱和脂肪酸的转基因植物。

59. 发明申请

US20090205066A1 FATTY ACID DESATURASE GENES FROM PLANTS 审中-公开
标题翻译：来自植物的脂肪酸去饱和基因
公开(公告)号：US20090205066A1
公开(公告)日：2009-08-13
申请号：US12420395
申请日：2009-04-08
申请人： John Browse , Luis Perez Grau , Anthony J. Kinney , John W. Pierce, JR. , Anna M. Wierzbicki , Narendra S. Yadav
发明人： John Browse , Luis Perez Grau , Anthony J. Kinney , John W. Pierce, JR. , Anna M. Wierzbicki , Narendra S. Yadav
IPC分类号： A01H1/00 , C07H21/04 , A01H5/00 , C11B1/00 , C12Q1/68
CPC分类号： C12N9/0083 , A01H5/10 , C12N15/8247 , C12Y114/19006
摘要： The preparation and use of nucleic acid fragments encoding fatty acid desaturase enzymes are described. The invention permits alteration of plant lipid composition. Chimeric genes incorporating such nucleic acid fragments with suitable regulatory sequences may be used to create transgenic plants with altered levels of unsaturated fatty acids.
摘要翻译：描述了编码脂肪酸去饱和酶的核酸片段的制备和使用。本发明允许改变植物脂质组成。使用合适的调控序列掺入此类核酸片段的嵌合基因可用于产生具有改变水平的不饱和脂肪酸的转基因植物。

60. 发明申请

US20090203142A1 NUCLEOTIDE SEQUENCES OF A NEW CLASS OF DIVERGED DELTA-9 STEAROYL-ACP DESATURASE GENES 审中-公开
标题翻译：一类新型三角形DELTA-9型硬脂酸脱氢酶基因的核苷酸序列
公开(公告)号：US20090203142A1
公开(公告)日：2009-08-13
申请号：US12357474
申请日：2009-01-22
申请人： John R. Booth , Rebecca E. Cahoon , William D. Hitz , Anthony J. Kinney , Narendra S. Yadav
发明人： John R. Booth , Rebecca E. Cahoon , William D. Hitz , Anthony J. Kinney , Narendra S. Yadav
IPC分类号： C12N15/82 , C07H21/04 , C12N15/63 , C40B30/04
CPC分类号： C12N9/0083
摘要： An isolated nucleic acid fragment encoding a diverged delta-9 fatty acid desaturase is disclosed. Also the construction of a chimeric gene encoding all or a portion of the diverged delta-9 fatty acid desaturase is disclosed, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the diverged delta-9 fatty acid desaturase in a transformed host cell.
摘要翻译：公开了一种编码发散的δ-9脂肪酸去饱和酶的分离的核酸片段。在正义或反义方向上也公开了编码全部或部分分歧的δ-9脂肪酸去饱和酶的嵌合基因的构建，其中嵌合基因的表达导致产生改变的发散的δ-9脂肪酸水平去饱和酶在转化的宿主细胞中。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式