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51. 发明授权

US06566070B2 Detection of disease related genes 失效
标题翻译：检测疾病相关基因
公开(公告)号：US06566070B2
公开(公告)日：2003-05-20
申请号：US09943120
申请日：2001-08-30
申请人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
发明人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
IPC分类号： C12Q168
CPC分类号： A61K41/0042 , A61K38/00 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6883 , G01N33/58
摘要： The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other maninials, and kits which may be used for the detection of diseases and disorders.
摘要翻译：本发明涉及用于在细胞或无细胞翻译系统中翻译的新生蛋白的非放射性标记，检测，定量和分离的方法。 tRNA分子被非放射性标记物错氨基酰化，其可以是非天然氨基酸，氨基酸类似物或衍生物，或由蛋白质合成机理识别的物质。标记可以包含可切割部分，可检测标记，报告基因特性，其中掺入蛋白质的标记可以与非组合标记物或促进从翻译系统的其他组分检测和分离新生蛋白质的偶联剂区别。本发明还包括使用可以用于治疗人类和其他肌肉疾病和病症的药物组合物中的使用错氨基酰化tRNA制备的蛋白质，以及可用于检测疾病和病症的试剂盒。

52. 发明授权

US06210941B1 Methods for the detection and isolation of proteins 有权
标题翻译：检测和分离蛋白质的方法
公开(公告)号：US06210941B1
公开(公告)日：2001-04-03
申请号：US09290325
申请日：1999-04-12
申请人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
发明人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
IPC分类号： C12N900
CPC分类号： A61K41/0042 , A61K38/00 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6883 , G01N33/58
摘要： The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may be used for the detection of diseases and disorders.
摘要翻译：本发明涉及用于在细胞或无细胞翻译系统中翻译的新生蛋白的非放射性标记，检测，定量和分离的方法。 tRNA分子被非放射性标记物错氨基酰化，其可以是非天然氨基酸，氨基酸类似物或衍生物，或由蛋白质合成机理识别的物质。标记可以包含可切割部分，可检测标记，报告基因特性，其中掺入蛋白质的标记可以与非组合标记物或促进从翻译系统的其他组分检测和分离新生蛋白质的偶联剂区别。本发明还包括使用可用于治疗人类和其他哺乳动物疾病和病症的药物组合物中的使用错氨基酰化tRNA制备的蛋白质，以及可用于检测疾病和病症的试剂盒。

53. 发明授权

US5643722A Methods for the detection and isolation of proteins 失效
标题翻译：检测和分离蛋白质的方法
公开(公告)号：US5643722A
公开(公告)日：1997-07-01
申请号：US240511
申请日：1994-05-11
申请人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
发明人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
IPC分类号： A61K38/00 , A61K41/00 , C07C205/45 , C07C205/57 , C07D207/40 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/107 , C07K1/13 , C12P21/00 , G01N33/532 , C12Q1/68
CPC分类号： C12Q1/6876 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , G01N33/532 , A61K38/00
摘要： The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may be used for the detection of diseases and disorders.
摘要翻译：本发明涉及用于在细胞或无细胞翻译系统中翻译的新生蛋白的非放射性标记，检测，定量和分离的方法。 tRNA分子被非放射性标记物错氨基酰化，其可以是非天然氨基酸，氨基酸类似物或衍生物，或由蛋白质合成机理识别的物质。标记可以包含可切割部分，可检测标记，报告基因特性，其中掺入蛋白质的标记可以与非组合标记物或促进从翻译系统的其他组分检测和分离新生蛋白质的偶联剂区别。本发明还包括使用可用于治疗人类和其他哺乳动物疾病和病症的药物组合物中的使用错氨基酰化tRNA制备的蛋白质，以及可用于检测疾病和病症的试剂盒。

54. 发明授权

US08852956B2 Method for diagnosing primary biliary cirrhosis (PBC) using novel autoantigens 有权
标题翻译：使用新型自身抗原诊断原发性胆汁性肝硬化（PBC）的方法
公开(公告)号：US08852956B2
公开(公告)日：2014-10-07
申请号：US13500411
申请日：2010-10-05
申请人： Mark J. Lim , Heather P. Ostendorf , Kenneth J. Rothschild , Donald B. Bloch
发明人： Mark J. Lim , Heather P. Ostendorf , Kenneth J. Rothschild , Donald B. Bloch
IPC分类号： G01N33/564 , G01N33/543
CPC分类号： G01N33/564 , G01N33/6893 , G01N2333/47 , G01N2800/085
摘要： Methods and compositions are described for the diagnosis of primary biliary cirrhosis. Novel autoantigens are described for use in assays which employ test samples from individuals.
摘要翻译：描述了用于诊断原发性胆汁性肝硬化的方法和组合物。描述了新的自身抗原用于使用来自个体的测试样品的测定中。

55. 发明申请

US20100075374A1 Methods for capturing nascent proteins 审中-公开
标题翻译：捕获新生蛋白的方法
公开(公告)号：US20100075374A1
公开(公告)日：2010-03-25
申请号：US12290783
申请日：2008-10-31
申请人： Mark J. Lim , Kenneth J. Rothschild
发明人： Mark J. Lim , Kenneth J. Rothschild
IPC分类号： C12P21/00
CPC分类号： C12P21/02
摘要： Methods of generating and capturing nascent proteins are described, including capturing nascent proteins on the same bead that comprises nucleic acid encoding the protein. The protein or nucleic acid can be sequenced before or after phototransfer to another surface.
摘要翻译：描述了产生和捕获新生蛋白质的方法，包括在包含编码蛋白质的核酸的相同珠粒上捕获新生蛋白质。蛋白质或核酸可以在光转移到另一个表面之前或之后进行测序。

56. 发明授权

US4802951A Method for parallel fabrication of nanometer scale multi-device structures 失效
标题翻译：纳米尺度多器件结构并联制作方法
公开(公告)号：US4802951A
公开(公告)日：1989-02-07
申请号：US136992
申请日：1987-12-23
申请人： Noel A. Clark , Kenneth Douglas , Kenneth J. Rothschild
发明人： Noel A. Clark , Kenneth Douglas , Kenneth J. Rothschild
IPC分类号： C40B40/10 , C40B60/14 , G03F7/004 , G03F7/038 , G03F7/039 , B44C1/22 , B05D3/06 , G03C5/00
CPC分类号： B82Y30/00 , G03F7/004 , G03F7/038 , G03F7/039 , B01J2219/00317 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： Articles exhibiting fabricated structures with nanometer size scale features (nanostructures), typically a device comprising nanostructures of a functional material on or in a substrate of dissimilar material, are produced by a method employing a substrate base or coating and a thin layer serving as a lithographic mask or template, consisting of a self-assembled ordered material array, typically a periodic array of molecules such as undenatured proteins, exhibiting holes, thickness or density variations. It is possible to produce complex structures containing large numbers of nanometers scale elements through a small number of simple steps.
摘要翻译：具有纳米尺度尺度特征（纳米结构）的制造结构的产品通常是通过使用基板基底或涂层的方法和用作光刻的薄层来制造的，其包括在不同材料的衬底上或衬底中的功能材料的纳米结构的器件掩模或模板，由自组装的有序材料阵列组成，通常是分子的周期性阵列，例如未变性的蛋白质，显示孔，厚度或密度变化。通过少量简单的步骤可以生产含有大量纳米尺度元件的复杂结构。

57. 发明授权

US4728591A Self-assembled nanometer lithographic masks and templates and method for parallel fabrication of nanometer scale multi-device structures 失效
标题翻译：自组装纳米光刻掩模和模板以及纳米尺度多器件结构的并联制作方法
公开(公告)号：US4728591A
公开(公告)日：1988-03-01
申请号：US837376
申请日：1986-03-07
申请人： Noel A. Clark , Kenneth Douglas , Kenneth J. Rothschild
发明人： Noel A. Clark , Kenneth Douglas , Kenneth J. Rothschild
IPC分类号： C40B40/10 , C40B60/14 , G03F7/004 , G03F7/038 , G03F7/039 , G03F9/00
CPC分类号： G03F7/004 , B82Y30/00 , G03F7/038 , G03F7/039 , B01J2219/00317 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00725 , C40B40/10 , C40B60/14 , Y10T428/261 , Y10T428/31681 , Y10T428/31855 , Y10T428/31942
摘要： Articles exhibiting fabricated structures with nanometer size scale features (nanostructures), typically a device comprising nanostructures of a functional material on or in a substrate of dissimilar material, are produced by a method employing a substrate base or coating and a thin layer serving as a lithographic mask or template, consisting of a self-assembled ordered material array, typically a periodic array of molecules such as undenatured proteins, exhibiting holes, thickness or density variations. It is possible to produce complex structures containing large numbers of nanometers scale elements through a small number of simple steps.
摘要翻译：具有纳米尺度尺度特征（纳米结构）的制造结构的产品通常是通过使用基板基底或涂层的方法和用作光刻的薄层来制造的，其包括在不同材料的衬底上或衬底中的功能材料的纳米结构的器件掩模或模板，由自组装的有序材料阵列组成，通常是分子的周期性阵列，例如未变性的蛋白质，显示孔，厚度或密度变化。通过少量简单的步骤可以生产含有大量纳米尺度元件的复杂结构。

58. 发明授权

US08906700B2 Methods and compositions for phototransfer 有权
标题翻译：光转染的方法和组合物
公开(公告)号：US08906700B2
公开(公告)日：2014-12-09
申请号：US13553472
申请日：2012-07-19
申请人： Mark J. Lim , Kenneth J. Rothschild
发明人： Mark J. Lim , Kenneth J. Rothschild
IPC分类号： G01N33/543 , G01N33/53 , C40B30/04
CPC分类号： G01N33/54306 , C12Q1/6806 , G01N33/54353 , G01N33/54393
摘要： Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.
摘要翻译：描述了将化合物从第一表面转移到第二表面的方法。用可光洁化的接头描述化合物。通过光可透洗接头连接到第一表面的化合物与第二表面接近（或接触），然后在暴露于电磁辐射时转移到第二表面。用辐射照射化合物使化合物从第一表面光化，并将化合物转移到第二表面。

59. 发明申请

US20140121363A1 Bioreactive Agents 失效
标题翻译：生物反应剂
公开(公告)号：US20140121363A1
公开(公告)日：2014-05-01
申请号：US13616499
申请日：2012-09-14
申请人： Kenneth J. Rothschild , Sanjay M. Sonar , Jarzy Olejelk
发明人： Kenneth J. Rothschild , Sanjay M. Sonar , Jarzy Olejelk
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , A61K38/00 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6823 , G01N33/5306 , G01N33/532 , G01N33/58 , C12Q2523/319
摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. The invention also relates to targets isolated with conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. The invention further relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and individual substances in a complex background of substances.
摘要翻译：本发明涉及可用于从复杂混合物检测和分离靶组分的试剂和缀合物，例如来自生物样品的核酸，来自体液的细胞和来自翻译反应的新生蛋白。本发明还涉及用缀合物分离的靶，其可用作可以施用于人和其它哺乳动物的药剂或组合物。本发明还涉及包含可用于检测物质复杂背景中的疾病，病症和个体物质的试剂和缀合物的试剂盒。

60. 发明申请

US20100311042A1 Amplifying bisulfite-treated template 有权
标题翻译：扩增亚硫酸氢盐处理的模板
公开(公告)号：US20100311042A1
公开(公告)日：2010-12-09
申请号：US12291178
申请日：2008-11-06
申请人： Mark J. Lim , Kenneth J. Rothschild
发明人： Mark J. Lim , Kenneth J. Rothschild
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844
摘要： Methods of amplifying nucleic acid are described. Primers on a solid support, e.g. a population of beads, are employed. A population of nucleic acid template molecules, wherein said nucleic acid template molecules have been treated with bisulfite, is amplified so as to create loaded beads comprising immobilized amplified nucleic acid.
摘要翻译：描述了扩增核酸的方法。固体支持物上的引物，例如一群珠子被雇用。扩增核酸模板分子群体，其中所述核酸模板分子已经用亚硫酸氢盐处理，以产生包含固定化扩增核酸的负载珠。

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