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    • 51. 发明授权
    • Purified plant expansin proteins and DNA encoding same
    • 纯化植物扩增蛋白和编码相同的DNA
    • US07226756B2
    • 2007-06-05
    • US10670009
    • 2003-09-24
    • Daniel J. CosgroveSimon McQueen-MasonMark J. GuiltinanTatyana ShcherbanJun Shi
    • Daniel J. CosgroveSimon McQueen-MasonMark J. GuiltinanTatyana ShcherbanJun Shi
    • C07K14/415C12N15/82C12N9/42C12N9/00
    • C07K14/415C12N9/00C12N15/8242C12N15/8261D21C5/027D21H17/22Y02A40/146Y02W30/648
    • This invention relates to a new class of proteins called expansins, and methods related thereto are presented. This class of proteins can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose. Two proteins have been isolated from washed wall fragments of cucumber hypocotyls, referred to as “cucumber expansin-29” and “cucumber expansin-30”. Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified in Arabidopsis and an additional two in rice.
    • 本发明涉及一类称为膨胀素的新型蛋白质,并提出了与之相关的方法。 这类蛋白质可以表征为植物细胞壁延伸的催化剂和纯纤维素中氢键的弱化。 已经从黄瓜下胚轴的洗涤的壁碎片中分离出两种蛋白质,称为“黄瓜扩展蛋白-29”和“黄瓜扩展蛋白30”。 此外,对来自纯化的cEx-29蛋白的三个肽片段进行测序,然后设计寡核苷酸引物以使用来自黄瓜幼苗的cDNA模板进行聚合酶链反应扩增一部分扩展蛋白cDNA,然后使用PCR片段筛选 用于鉴定全长克隆的cDNA文库。 另外一种扩增蛋白已经从燕麦胚芽鞘(oat expansin oEx-29)中分离出来,而在拟南芥中已经鉴定了三种扩增蛋白序列,另外两种在水稻中被鉴定出来。
    • 52. 发明申请
    • Method and apparatus for recording a signal
    • 用于记录信号的方法和装置
    • US20060165374A1
    • 2006-07-27
    • US10564296
    • 2004-07-01
    • Philip NewtonDeclan KellyJingwei TanLiang GanJun Shi
    • Philip NewtonDeclan KellyJingwei TanLiang GanJun Shi
    • H04N5/91
    • H04N5/9201G11B27/034G11B27/036G11B27/105G11B27/3027G11B2220/216G11B2220/218G11B2220/2545G11B2220/2562H04N5/781H04N5/85H04N5/907
    • The invention relates to a system for recording a signal. A recorder (200) comprises a receiver (201) for receiving a source signal (101) having associated first play time information such as a Normal Play Time timeline. The receiver (200) is coupled to a recording controller (203) which generates a recorded signal (301) from the source signal (101). The recorded signal (301) comprises a recording discontinuity with respect to the source signal (101) caused by for example the recording being paused in a time interval. The recording controller (203) is coupled to a storage medium (205). The recorder (200) further comprises a time processor (209) for generating second time information for the recorded signal (301) in response to the first play time information and the recording discontinuity. The second time information may for example be a compensated NPT timeline or event descriptors or stream events having time indications modified to correspond to the recorded signal (301).
    • 本发明涉及用于记录信号的系统。 记录器(200)包括用于接收具有相关联的第一播放时间信息(例如正常播放时间时间线)的源信号(101)的接收器(201)。 接收器(200)耦合到从源信号(101)产生记录信号(301)的记录控制器(203)。 所记录的信号(301)包括关于源信号(101)的记录不连续性,例如由时间间隔暂停的记录引起的。 记录控制器(203)耦合到存储介质(205)。 记录器(200)还包括响应于第一播放时间信息和记录不连续性而产生记录信号(301)的第二时间信息的时间处理器(209)。 第二时间信息可以例如是补偿的NPT时间线或事件描述符或具有修改为对应于所记录的信号的时间指示的事件描述符或流事件(301)。
    • 54. 发明授权
    • Proteins catalyzing the extension of plant cell walls
    • 催化植物细胞壁延伸的蛋白质
    • US5959082A
    • 1999-09-28
    • US440517
    • 1995-05-12
    • Daniel J. CosgroveSimon McQueen-MasonMark GuiltinanTatyana ShcherbanJun Shi
    • Daniel J. CosgroveSimon McQueen-MasonMark GuiltinanTatyana ShcherbanJun Shi
    • C07K14/415C12N9/00C12N9/42C12N15/82D21H17/22A61K35/78A23J1/14C07K17/00
    • C12N9/00C07K14/415C12N15/8241C12N15/8242C12N15/8261C12N9/2437D21H17/22
    • A new class of proteins and methods related thereto are presented. The proteins, which can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose, are referred to as expansins. Two proteins have been isolated by fractionation techniques from washed wall fragments of cucumber hypocotyls, referred to as "cucumber expansin-29" and "cucumber expansin-30" (abbreviated cEx-29 and cEx-30, with respect to their apparent relative masses as determined by SDS-PAGE). Moreover, three peptide fragments from the purified cEx-29 protein were sequenced, then oligonucleotide primers were designed to amplify a portion of the expansin cDNA using polymerase chain reaction with a cDNA template derived from cucumber seedlings, and then the PCR fragment was used to screen a cDNA library to identify full length clones. Another expansin protein has been isolated from oat coleoptiles (oat expansin oEx-29), while three additional expansin sequences have been identified in Arabidopsis and an additional two in rice. Expansins appear to be broadly distributed throughout the plant kingdom and can be identified in stem and leaf vegetables (i.e., broccoli, cabbage), fruit and seed vegetables (i.e., tomato), fiber crops and cereals (i.e., corn), and forest and ornamental crops (i.e., cotton). An expansin, generally, is a protein which has at least about 60% sequence similarity with the amino acid sequence shown in SEQ. ID. NO:1, and preferably has at least about 70% sequence similarity with SEQ. ID. NO:1.
    • 提出了一类新的相关蛋白质和方法。 可以表征为植物细胞壁延伸的催化剂和纯纤维素中氢键的弱化的蛋白质被称为膨胀物。 通过分离技术从黄瓜下胚轴的洗涤壁片段(称为“黄瓜扩展蛋白-29”和“黄瓜扩展子30”)(缩写为cEx-29和cEx-30),通过分离技术分离了两种蛋白质,其相对于其表观相对质量为 通过SDS-PAGE测定)。 此外,对来自纯化的cEx-29蛋白的三个肽片段进行测序,然后设计寡核苷酸引物以使用来自黄瓜幼苗的cDNA模板进行聚合酶链反应扩增一部分扩展蛋白cDNA,然后使用PCR片段筛选 一个cDNA文库,用于鉴定全长克隆。 另外一种扩增蛋白已经从燕麦胚芽鞘(oat expansin oEx-29)中分离出来,而在拟南芥中已经鉴定了三种扩增蛋白序列,另外两种在水稻中被鉴定出来。 扩展体似乎广泛分布在整个植物界,可以在茎叶蔬菜(即西兰花,卷心菜),水果和种子蔬菜(即番茄),纤维作物和谷类(即玉米),以及森林和 观赏作物(即棉花)。 通常,扩展蛋白是与SEQ ID NO:1所示氨基酸序列具有至少约60%序列相似性的蛋白质。 ID。 NO:1,优选与SEQ ID NO:1至少约70%的序列相似性。 ID。 NO:1。
    • 59. 发明授权
    • Segmented ceramic matrix composite turbine airfoil component
    • 分段陶瓷基体复合涡轮机翼型件
    • US08511980B2
    • 2013-08-20
    • US13555877
    • 2012-07-23
    • Tracy A. Propheter-HinckleyJun Shi
    • Tracy A. Propheter-HinckleyJun Shi
    • F01D9/02
    • F01D5/284F01D5/3015F05D2240/80Y02T50/672Y02T50/673
    • A segmented component for use with a gas turbine engine comprises a radially extending gas path portion. The gas path portion is for interacting with gas flow from the gas turbine engine. The gas path portion comprises a forward portion forming a leading edge of a stationary vane, an aft portion forming a trailing edge of the stationary vane, and a plurality of middle portions forming a pressure side and a suction side of the stationary vane. The component is divided into axially aligned segments comprising a forward segment, an aft segment, and a plurality of middle segments disposed between the forward segment and the aft segment. The middle segments comprise radially elongate ceramic matrix composite material plates.
    • 用于燃气涡轮发动机的分段部件包括径向延伸的气体路径部分。 气体路径部分用于与来自燃气涡轮发动机的气体流相互作用。 气体路径部包括形成静止叶片的前缘的前部,形成静止叶片的后缘的后部,以及形成静止叶片的压力侧和吸力侧的多个中间部。 该部件被分成轴向对准的段,其包括前段,后段和设置在前段和后段之间的多个中段。 中间段包括径向细长的陶瓷基复合材料板。