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51. 发明授权

US07897386B2 Cell-based assays for G-protein-coupled receptor-mediated activities 有权
标题翻译：用于G蛋白偶联受体介导的活性的基于细胞的测定
公开(公告)号：US07897386B2
公开(公告)日：2011-03-01
申请号：US12124717
申请日：2008-05-21
申请人： Yong Yao , Liang Cao
发明人： Yong Yao , Liang Cao
IPC分类号： C12N5/10 , C07K14/705
CPC分类号： G01N33/566 , G01N2333/726 , G01N2500/02 , G01N2500/04 , G01N2500/10
摘要： Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.
摘要翻译：公开了其用途的组合物和方法，例如鉴定G-蛋白偶联受体，调节G蛋白偶联受体活性的配体和化合物。组合物和方法使用环状核苷酸门控通道和荧光染料来检测响应于G蛋白偶联受体的刺激的细胞内cAMP水平的变化。可以在多种测定中检测G蛋白偶联受体的激活，包括使用荧光显微镜的基于细胞的成像测定和具有多孔板和荧光板读数器的高通量测定。

52. 发明授权

US07604959B2 Cell-based assays employing voltage and calcium dyes 有权
标题翻译：使用电压和钙染料的基于细胞的测定
公开(公告)号：US07604959B2
公开(公告)日：2009-10-20
申请号：US11783884
申请日：2007-04-12
申请人： Yong Yao , Liang Cao , Jianming Lu , Isabel Llorente
发明人： Yong Yao , Liang Cao , Jianming Lu , Isabel Llorente
IPC分类号： C12N15/12 , C07K14/705
CPC分类号： C07K14/705 , G01N33/566 , G01N2333/726 , G01N2500/02 , G01N2500/04 , G01N2500/10
摘要： Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.
摘要翻译：公开了使用环状核苷酸门控通道和荧光染料以及其他指示剂来检测细胞内cAMP水平的变化的新型组合物和方法，例如响应于G蛋白偶联受体的刺激。包括一种或多种增加对cAMP的敏感性和减少二价阳离子介导的阻断的孔突变的CNG突变体用于多种测定，包括G蛋白偶联受体活性的检测。还公开了特别适用于蛋白质相互作用的基于细胞的测定的新型染料猝灭剂制剂。

53. 发明申请

US20080166803A1 Novel cell-based assays employing voltage and calcium dyes 有权
标题翻译：使用电压和钙染料的新型基于细胞的测定
公开(公告)号：US20080166803A1
公开(公告)日：2008-07-10
申请号：US11783884
申请日：2007-04-12
申请人： Yong Yao , Liang Cao , Jianming Lu , Isabel Llorente
发明人： Yong Yao , Liang Cao , Jianming Lu , Isabel Llorente
IPC分类号： C12N5/10 , C07H21/00 , C12N5/06 , C12N5/08
CPC分类号： C07K14/705 , G01N33/566 , G01N2333/726 , G01N2500/02 , G01N2500/04 , G01N2500/10
摘要： Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.
摘要翻译：公开了使用环状核苷酸门控通道和荧光染料以及其他指示剂来检测细胞内cAMP水平的变化的新型组合物和方法，例如响应于G蛋白偶联受体的刺激。包括一种或多种增加对cAMP的敏感性和减少二价阳离子介导的阻断的孔突变的CNG突变体用于多种测定，包括G蛋白偶联受体活性的检测。还公开了特别适用于蛋白质相互作用的基于细胞的测定的新型染料猝灭剂制剂。

54. 发明申请

US20080101362A1 METHOD AND DEVICE FOR MAKING UPLINK STANDBY 有权
标题翻译：用于制作UPLINK STANDBY的方法和设备
公开(公告)号：US20080101362A1
公开(公告)日：2008-05-01
申请号：US11965830
申请日：2007-12-28
申请人： Jiehui Hu , Bin Ren , Yu Xiong , Liang Cao , Nan Huai
发明人： Jiehui Hu , Bin Ren , Yu Xiong , Liang Cao , Nan Huai
IPC分类号： H04L12/56
CPC分类号： H04L45/00 , H04L12/1886 , H04L41/0668 , H04L43/0811 , H04L45/16 , H04L45/22 , H04L45/28 , H04L69/40
摘要： The embodiments of present invention disclose a method and a device for making uplink standby, including: configuring more than one uplink for an Internet Group Management Protocol (IGMP) proxy device, and choosing one of the more than one uplink as a primary uplink; the IGMP proxy device replicating a multicast packet received over the primary uplink and sending the multicast packet replicated over a downlink to a multicast host. In the method provided by the embodiments of the present invention, only the primary uplink is used, which reduces the link bandwidth waste of the multicast host and improves the multicast data transmission efficiency. Flexible methods are adopted for checking the availability of the primary uplink so that the IGMP proxy device may detect in real time whether the primary uplink is available and choose a new primary uplink in time to guarantee the normal multicast communication.
摘要翻译：本发明的实施例公开了一种用于进行上行备用的方法和装置，包括：为因特网组管理协议（IGMP）代理设备配置多于一个上行链路，并选择多于一个上行链路中的一个作为主上行链路; IGMP代理设备复制通过主上行链路接收的组播分组，并将通过下行链路复制的组播分组发送给组播主机。在本发明实施例提供的方法中，仅使用主上行链路，减少了多播主机的链路带宽浪费，提高了组播数据的传输效率。采用灵活的方法来检查主上行链路的可用性，使得IGMP代理设备可以实时检测主上行链路是否可用，并及时选择新的主上行链路，以保证正常的组播通信。

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