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51. 发明授权

US06670166B1 Arthropod protein disulfide isomerases 失效
标题翻译：节肢动物蛋白二硫键异构酶
公开(公告)号：US06670166B1
公开(公告)日：2003-12-30
申请号：US09807258
申请日：2001-04-09
申请人： Rebecca E. Cahoon , Rafael Herrmann , Albert L. Lu
发明人： Rebecca E. Cahoon , Rafael Herrmann , Albert L. Lu
IPC分类号： C12N990
CPC分类号： C12N15/8286 , C12N9/90 , C12N15/8257 , Y02A40/162
摘要： This invention relates to an isolated nucleic acid fragment encoding protein disulfide isomerase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the protein disulfide isomerase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the protein disulfide isomerase in a transformed host cell.
摘要翻译：本发明涉及编码蛋白质二硫键异构酶的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码全部或部分蛋白质二硫键异构酶的嵌合基因，其中嵌合基因的表达导致转化的宿主细胞中蛋白质二硫键异构酶水平的改变。

52. 发明授权

US07910807B2 Plant activation of insect toxin 有权
标题翻译：植物激活昆虫毒素
公开(公告)号：US07910807B2
公开(公告)日：2011-03-22
申请号：US11940002
申请日：2007-11-14
申请人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
IPC分类号： C12N15/82 , C12N15/32 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

53. 发明申请

US20090265818A1 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA 有权
标题翻译：用于抑制来自LEPIDOPTERA的目标多核苷酸的组合物和方法
公开(公告)号：US20090265818A1
公开(公告)日：2009-10-22
申请号：US12351267
申请日：2009-01-09
申请人： Rafael Herrmann , Michael Lassner , Albert L. Lu , Mark Nelson , James K. Presnail , Janet A. Rice
发明人： Rafael Herrmann , Michael Lassner , Albert L. Lu , Mark Nelson , James K. Presnail , Janet A. Rice
IPC分类号： A01H5/00 , C12N5/04 , A01N43/04 , A01P7/04
CPC分类号： C12N15/8286 , A01N65/20 , A01N65/44 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/531 , C12N2320/32 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当沉积元件在被害虫如来自鳞翅目序列的害虫中摄取时，它们能够降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供编码来自特定蛋白质家族的多肽的多核苷酸和SEQ ID NO：1,2,3,4,5,6,7,8,9,10,11,12,13,14中所示的各种靶多核苷酸的靶多核苷酸，15，16，17，18，19，20，21，22，23，24，25，26，27，28，29，30，31，32，33，34，35，36，37，38，39 ，40,41,42,43,44,45,46,47,48,49或50个或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即，具有杀虫活性）。进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是草地夜蛾（Spodoptera frugiperda）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

54. 发明申请

US20090192117A1 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LYGUS 有权
标题翻译：用于抑制来自LYGUS的目标多核苷酸的组合物和方法
公开(公告)号：US20090192117A1
公开(公告)日：2009-07-30
申请号：US12351379
申请日：2009-01-09
申请人： Rafael Herrmann , Michael Lassner , Albert L. Lu , Mark E. Nelson , James K. Presnail , Janet A. Rice
发明人： Rafael Herrmann , Michael Lassner , Albert L. Lu , Mark E. Nelson , James K. Presnail , Janet A. Rice
IPC分类号： A61K31/7088 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8286 , A01N57/16 , C12N15/113 , C12N15/8218 , C12N2310/141 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, and further provides target polynucleotides set forth in SEQ ID NOS:1-21 or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Lygus Hesperus. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当沉积元件被害虫摄取时，例如来自莱古氏属属的害虫时，它们能够降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了本文公开的来自特定多肽家族的多种靶多核苷酸，并进一步提供了SEQ ID NO：1-21所示的靶多核苷酸或其活性变体，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，有害生物是黄瓜（Lygus Hesperus）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

55. 发明授权

US07556936B2 Exogenous proteases for plant activation of insect toxin 失效
标题翻译：植物激活昆虫毒素
公开(公告)号：US07556936B2
公开(公告)日：2009-07-07
申请号：US11950509
申请日：2007-12-05
申请人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
IPC分类号： C12N9/64 , C12N15/57 , C12N15/82 , C12Q1/37
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。本发明的具体实施方案进一步提供改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

56. 发明申请

US20090011999A1 ORALLY ACTIVE ANDROCTONUS AMOREUXI PESTICIDAL BIOPEPTIDES 审中-公开
公开(公告)号：US20090011999A1
公开(公告)日：2009-01-08
申请号：US12171822
申请日：2008-07-11
申请人： Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , James F.H. Wong
发明人： Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , James F.H. Wong
IPC分类号： A01N63/00 , C07K14/00 , A01P15/00
CPC分类号： C12N15/8286 , C07K14/43522 , Y02A40/162
摘要： The present invention provides compositions and methods for orally active Androctonus amoreuxi pesticidal polypeptides. Compositions include novel Androctonus amoreuxi pesticidal polypeptides and biologically active variants thereof. Further provided are methods for modulating the pesticide resistance of plants by expressing the sequences disclosed herein. One method comprises stably transforming into the genome of a plant cell a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence. An additional method comprises incorporating a nucleotide sequence of the present invention operably linked to a heterologous promoter into a microorganism and applying said microorganism to the environment of a plant.
摘要翻译：本发明提供了口服活性黄芪杀虫多肽的组合物和方法。组合物包括新颖的安德龙（Amroctonus amoreuxi）杀虫多肽及其生物活性变体。还提供了通过表达本文公开的序列调节植物的农药抗性的方法。一种方法包括稳定地转化到植物细胞的基因组中，本发明的核苷酸序列与异源启动子可操作地连接并再生表达核苷酸序列的稳定转化的植物。另外的方法包括将可操作地连接到异源启动子的本发明的核苷酸序列并入微生物并将所述微生物施用于植物的环境。

57. 发明授权

US07473821B2 Nucleic acids encoding Cry8Bb1 endotoxins engineered to have insect-specific protease recognition sequences 有权
标题翻译：编码Cry8Bb1内毒素的核酸被改造为具有昆虫特异性蛋白酶识别序列
公开(公告)号：US07473821B2
公开(公告)日：2009-01-06
申请号：US11448266
申请日：2006-06-07
申请人： Andre R. Abad , Gary C. Chun , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Janet A. Rice , Eric J. Schepers
发明人： Andre R. Abad , Gary C. Chun , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Janet A. Rice , Eric J. Schepers
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

58. 发明授权

US07232939B2 Nucleic acid molecules encoding cyclotide polypeptides and methods of use 有权
标题翻译：编码环肽多肽的核酸分子及其使用方法
公开(公告)号：US07232939B2
公开(公告)日：2007-06-19
申请号：US11129817
申请日：2005-05-16
申请人： Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Clement W. K. Waine
发明人： Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Clement W. K. Waine
IPC分类号： C12N15/09 , C12N15/29 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8285 , C07K14/415 , C12N15/8282 , Y02A40/164
摘要： The present invention relates to isolated nucleic acids encoding plant cyclotides. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
摘要翻译：本发明涉及编码植物环肽的分离的核酸。本发明还涉及在正义或反义方向上构建编码全部或部分植物环肽的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的植物环磷酰胺。

59. 发明申请

US20070157338A1 Insecticidal Plant Cyclotide with Activity Against Homopteran Insects 失效
公开(公告)号：US20070157338A1
公开(公告)日：2007-07-05
申请号：US11677644
申请日：2007-02-22
申请人： Rafael Herrmann , Albert Lu , Billy McCutchen , James Presnail , Janet Rice , Clement Waine
发明人： Rafael Herrmann , Albert Lu , Billy McCutchen , James Presnail , Janet Rice , Clement Waine
IPC分类号： A01H5/00 , C12N15/82 , C12N5/04
CPC分类号： C07K14/415 , C12N15/8286 , Y02A40/162
摘要： The present invention relates to isolated nucleic acids encoding plant cyclotides. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.

60. 发明申请

US20050188440A1 RHIZOC3 nucleic acid molecules 失效
标题翻译： RHIZOC3核酸分子
公开(公告)号：US20050188440A1
公开(公告)日：2005-08-25
申请号：US11093088
申请日：2005-03-29
申请人： Daniel Altier , Rafael Herrmann , Albert Lu , Billy McCutchen , James Presnail , Janine Weaver , James Wong
发明人： Daniel Altier , Rafael Herrmann , Albert Lu , Billy McCutchen , James Presnail , Janine Weaver , James Wong
IPC分类号： C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N7/01 , C12N15/12 , C12N15/82 , A01H1/00 , C07H21/04 , C12N5/04 , C12N7/00
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.
摘要翻译：本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。进一步提供了用本发明的核酸分子转化的植物细胞，植物和种子，以赋予植物抗病性。

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