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41. 发明申请

US20100075380A1 Novel Lipase 有权
标题翻译：新型脂肪酶
公开(公告)号：US20100075380A1
公开(公告)日：2010-03-25
申请号：US12096574
申请日：2006-12-08
申请人： Masahiro Nakao , Masaki Kanamori , Harukazu Fukami , Hiroaki Kasai , Misa Ochiai
发明人： Masahiro Nakao , Masaki Kanamori , Harukazu Fukami , Hiroaki Kasai , Misa Ochiai
IPC分类号： C07H21/04 , C12N15/63 , C12N1/21 , C07K14/195 , C12N9/20 , C12P7/64 , C12P21/00 , C12N5/10 , C12N1/19
CPC分类号： C12N9/20 , C12P7/6409 , C12P7/6436 , C12R1/01
摘要： The present invention provides a novel lipase with a molecular weight of about 32 kDa, which is produced by a strain belonging to the genus Tetrasphaera, as well as a gene encoding the same. This lipase has the ability to recognize a medium-chain fatty acid as a substrate. The present invention also provides a novel lipase with a molecular weight of about 40 kDa, which is produced by a strain belonging to the genus Tetrasphaera and has the ability to recognize both a medium-chain fatty acid and a long-chain fatty acid as substrates, as well as a polynucleotide encoding the same. The present invention further provides Tetrasphaera sp. strain NITE P-154. The lipase of the present invention can be used as an immobilized enzyme and is useful in fields such as production of digestants and/or flavorings, production of clinical laboratory reagents, detergent enzymes and/or fats, as well as production of optically active intermediates for agricultural chemicals and pharmaceutical preparations.
摘要翻译：本发明提供了一种分子量约32kDa的新型脂肪酶，其由属于四联属属的菌株产生，以及编码该脂肪酶的基因。该脂肪酶具有识别中链脂肪酸作为底物的能力。本发明还提供一种分子量约40kDa的新型脂肪酶，其由属于四孢属属的菌株产生，并且具有将中链脂肪酸和长链脂肪酸同时识别为底物的能力，以及编码该多核苷酸的多核苷酸。本发明进一步提供了Tetrasphaera sp。菌株NITE P-154。本发明的脂肪酶可以用作固定化酶，可用于生产消化剂和/或调味剂，生产临床实验室试剂，洗涤剂和/或脂肪等领域，以及生产光学活性中间体农药和药物制剂。

42. 发明申请

US20070072275A1 Method for breeding lipid-producing fungus 审中-公开
标题翻译：滋生真菌的方法
公开(公告)号：US20070072275A1
公开(公告)日：2007-03-29
申请号：US10569103
申请日：2004-08-20
申请人： Misa Ochiai , Hiroshi Kawashima , Sakayu Shimizu
发明人： Misa Ochiai , Hiroshi Kawashima , Sakayu Shimizu
IPC分类号： C12P7/64 , C12N15/74 , C12N1/18
CPC分类号： C12P7/6463 , C12N15/80
摘要： It is intended to provide a breeding method whereby a lipid-producing fungus belonging to the genus Mortierella can be effectively and efficiently bred without resort to antibiotics. Transformation is carried out with the use of an auxotroph (for example, uracil auxotroph) of a fungus belonging to the Mortierella as a host. More specically speaking, a gene compensating for the auxotrophy, which is employed as a marker, is transferred into the host (the gene transfer step). Next, a transformant is selected by using the recovery from the auxotrophy as the marker (the selection step). The lipid-producing fungus may be M. aplina, for example.
摘要翻译：旨在提供一种育种方法，其中可以有效地和有效地繁殖属于被孢霉属的产生脂质的真菌而不诉诸抗生素。使用属于被孢霉属的真菌的营养缺陷型（例如尿嘧啶营养缺陷型）作为宿主进行转化。更具体地说，补充用作标记的营养缺陷型的基因被转移到宿主（基因转移步骤）中。接下来，通过使用来自营养缺陷型的恢复作为标记来选择转化体（选择步骤）。例如，产生脂质的真菌可以是甲基纤维素。

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