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    • 45. 发明授权
    • Synthetic polymer particles for use as standards and calibrators in flow
cytometry
    • 合成聚合物颗粒,用作流式细胞仪中的标准品和校准品
    • US6074879A
    • 2000-06-13
    • US099233
    • 1998-06-17
    • David ZelmanovicWilliam MoskalskiRonny DeClercqFrank LouwetEberhard KuckertWolfgang Podszun
    • David ZelmanovicWilliam MoskalskiRonny DeClercqFrank LouwetEberhard KuckertWolfgang Podszun
    • G01N33/49G01N15/00G01N15/10G01N15/14G01N31/00
    • G01N15/1012G01N2015/1062G01N2015/1486G01N2015/149Y10T436/10Y10T436/101666
    • The invention describes novel methods for calibrating or standardizing flow cytometry instruments using synthetic polymer particles or beads having physical properties which provide advantages for their use in such instruments. The polymer particles or beads employed in the calibration methods are spherical, have an average particle diameter of from about 1 to about 8 microns, a relatively narrow particle distribution, and a low refractive index, i.e., from about 1.35 to about 1.45. The particles are safe, stable for long time periods and are particularly suitable for employment as hematology analyzer calibration substances. The invention provides an advantageous improvement over the types of standards currently used to calibrate a variety of instruments that rely on light scatter combined with other parameters to identify and measure biological particles or cells, as well as nonbiological particles. In particular, using the calibration/standardization method and polymer particles according to the present invention allows hematology instruments to be calibrated and standardized for subsequent accurate and reliable determinations of red blood cells, reticulocytes, white blood cells and platelets in whole blood samples.
    • 本发明描述了使用具有物理性质的合成聚合物颗粒或珠粒来校准或标准化流式细胞仪器的新方法,其提供了在这些仪器中使用的优点。 在校准方法中使用的聚合物颗粒或珠粒是球形的,平均粒径为约1至约8微米,相对较窄的颗粒分布和低折射率,即约1.35至约1.45。 颗粒安全,长时间稳定,特别适合用作血液分析仪校准物质。 本发明提供了相对于目前用于校准依赖于光散射的各种仪器与其他参数相结合以识别和测量生物颗粒或细胞以及非生物颗粒的标准类型的有利改进。 特别地,使用根据本发明的校准/标准化方法和聚合物颗粒允许血液仪器被校准和标准化,用于随后准确可靠地测定全血样品中的红细胞,网织红细胞,白细胞和血小板。
    • 50. 发明授权
    • Heat-sensitive recording material
    • 热敏记录材料
    • US5753587A
    • 1998-05-19
    • US715196
    • 1996-09-18
    • Wolfgang PodszunLuc Leenders
    • Wolfgang PodszunLuc Leenders
    • B41M5/28B41M5/30B41M5/323B41M5/40G03C1/498B41M5/34
    • G03C1/4989
    • A heat-sensitive image recording material contains on a support material 1. at least one binder layer with a heat-sensitive imaging combination of an organic silver salt and an organic reducing agent for the organic silver salt, and 2. at least one binder layer or a sequence of binder layers with a heat-sensitive imaging combination of an acid-sensitive leuco dye and an acidically reacting compound which acts as a developer for the leuco dye, wherein the leuco dye and developer are in thermal interaction with each other either in different layers or separately from each other in the same layer, characterised in that a layer with a water- or alcohol-soluble polymer is arranged between the combination of organic silver salt and organic reducing agent for the organic silver salt, on the one hand, and the combination of leuco dye and acidically reacting compound, on the other. The image recording material is suitable for recording thermal images with elevated optical density, good grey scale reproduction and good stability.
    • 热敏图像记录材料包含在支撑材料1上的至少一个粘合剂层,其具有有机银盐和有机银盐的有机还原剂的热敏成像组合,以及2.至少一种粘合剂层 或具有酸敏感无色染料和用作无色染料的显色剂的酸反应化合物的热敏成像组合的粘合剂层序列,其中所述无色染料和显影剂彼此热相互作用 不同层或在相同层中彼此分开,其特征在于在有机银盐和有机银盐的有机还原剂的组合之间布置具有水或醇可溶性聚合物的层,一方面, 和无色染料与酸反应的化合物的组合。 图像记录材料适用于记录光学密度高,灰度繁殖好,稳定性好的热像。